Infrared synchrotron radiation from bending magnet and edge radiation sources for the study of orientation and conformation in anisotropic materials

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A synchrotron-based infrared microspectroscopy study on the cellular response induced by gold nanoparticles combined with X-ray irradiations on F98 and U87-MG glioma cell lines

The inclusion of nanoparticles (NP) in radiotherapy has been shown to increase the damaging effect on tumor cells. However, the mechanisms of action of NP combined with radiotherapy, and the influence of NP parameters and cell type on their radiosensitization capability at molecular and cellular levels still remain unclear. Gold NP (AuNP) have become particularly popular due to their multiple advantages. Within this context, our research work aimed to study the biochemical radiosensitization capacity of F98 and U87-MG glioma cell lines to 1.9 nm AuNP combined with X-ray irradiation. For this purpose, synchrotron-based infrared microspectroscopy (SR-FTIRM) was used as a powerful tool for biochemical composition and treatment response assessment of cells at a single-cell level. SR-FTIRM data, supported by multivariate analysis, revealed clear AuNP-induced changes in the DNA, protein and lipid spectral regions. The AuNP-related biochemical alterations appear prior to the irradiation, which gave us a first indication on the AuNP radiosensitization action. Biochemical modifications induced by the AuNP in the presence of radiotherapy irradiations include enhanced conformational changes in the protein secondary structures, variations in the intensity and position in the phosphodiester bands, and changes in the CH2 and CH3 stretching modes. These changes are better manifested at 24 hours post-irradiation time. SR-FTIRM results showed a clear heterogeneity in the biochemical cell response, probably due to the distinct cell-NP interactions and thus, to different DNA damage and cell death processes

Boosted PTS Method with Mu-Law Companding Techniques for PAPR Reduction in OFDM Systems

This paper proposes an enhanced PAPR reduction technique which combines an enhanced PTS method with Mu-Law companding. The enhanced PTS method improves performances in both the partitioning and phase rotation steps. Enhancement in partitioning is achieved through a judicious incorporation of AP-PTS scheme into the IP-PTS. As for phase rotation, an optimal set of rotation vectors is derived based on the correlation properties of candidate signals. The PAPR reduction of this enhanced PTS method is further improved by annexing Mu-Law companding at the end of the enhanced PTS. This application of Mu-Law characteristic in the time domain of OFDM signal significantly improves the PAPR reduction capability of the approach. Simulation results show that the PAPR performance of the enhanced PTS method with Mu-Law companding technique on various scenarios with different modulation schemes is better than that of the PRP-PTS. This approach can be considered as a very attractive candidate for achieving a significant reduction of PAPR, while maintaining a low computational complexity

Non-isothermal crystallization kinetics of poly(4-hydroxybutyrate) biopolymer

The non-isothermal crystallization of the biodegradable poly(4-hydroxybutyrate) (P4HB) has been studied by means of differential scanning calorimetry (DSC) and polarizing optical microscopy (POM). In the first case, Avrami, Ozawa, Mo, Cazé, and Friedman methodologies were applied. The isoconversional approach developed by Vyazovkin allowed also the determination of a secondary nucleation parameter of 2.10 × 105 K2 and estimating a temperature close to 10 °C for the maximum crystal growth rate. Similar values (i.e., 2.22 × 105 K2 and 9 °C) were evaluated from non-isothermal Avrami parameters. All experimental data corresponded to a limited region where the polymer crystallized according to a single regime. Negative and ringed spherulites were always obtained from the non-isothermal crystallization of P4HB from the melt. The texture of spherulites was dependent on the crystallization temperature, and specifically, the interring spacing decreased with the decrease of the crystallization temperature (Tc). Synchrotron data indicated that the thickness of the constitutive lamellae varied with the cooling rate, being deduced as a lamellar insertion mechanism that became more relevant when the cooling rate increased. POM non-isothermal measurements were also consistent with a single crystallization regime and provided direct measurements of the crystallization growth rate (G). Analysis of the POM data gave a secondary nucleation constant and a bell-shaped G-Tc dependence that was in relative agreement with DSC analysis. All non-isothermal data were finally compared with information derived from previous isothermal analysesPeer ReviewedPostprint (published version

Synchrotron Radiation-Fourier transformed infrared microspectroscopy (μSR-FTIR) reveals multiple metabolism alterations in microalgae induced by cadmium and mercury

Toxic metals such as cadmium (Cd) and mercury (Hg) represent a threat to photosynthetic organisms of polluted aquatic ecosystems, and knowledge about mechanisms of toxicity is essential for appropriate assessment of environmental risks. We used Synchrotron Radiation-Fourier Transformed Infrared microspectroscopy (μSR-FTIR) to characterise major changes of biomolecules caused by Cd and Hg in the model green microalga Chlamydomonas reinhardtii. μSR-FTIR showed several metabolic alterations in different biochemical groups such as carbohydrates, proteins, and lipids in a time-dose dependent manner, with the strongest changes occurring at concentrations above 10 μM Cd and 15 μM Hg after short-term (24 h) treatments. This occurred in a context where metals triggered intracellular oxidative stress and chloroplast damage, along with autophagy induction by overexpressing AUTOPHAGY-RELATED PROTEIN 8 (ATG8). Thin layer chromatography analysis confirmed that toxic metals promoted remarkable changes in lipid profile, with higher degree of esterified fatty acid unsaturation as detected by gas chromatography coupled with mass spectrometry. Under Cd stress, there was specifically higher unsaturation of free fatty acids, while Hg led to stronger unsaturation in monogalactosyldiacylglycerol. μSR-FTIR spectroscopy proved as a valuable tool to identify biochemical alterations in microalgae, information that could be exploited to optimise approaches for metal decontaminationWork supported by the Spanish State Research Agency (AEI) (Spain) (projects AGL2014–53771-R and AGL2017–87591-R). The FTIR experiments were performed at MIRAS beamline at ALBA Synchrotron with the collaboration of ALBA staff and supported by travel grants for experiments No 2016091860 and 201702211

Biobased Terpene Derivatives: Stiff and Biocompatible Compounds to Tune Biodegradability and Properties of Poly(butylene succinate)

Different copolymers incorporating terpene oxide units (e.g., limonene oxide) have been evaluated considering thermal properties, degradability, and biocompatibility. Thus, polycarbonates and polyesters derived from aromatic, monocyclic and bicyclic anhydrides have been considered. Furthermore, ring substitution with myrcene terpene has been evaluated. All polymers were amorphous when evaluated directly from synthesis. However, spherulites could be observed after the slow evaporation of diluted chloroform solutions of polylimonene carbonate, with all isopropene units possessing an R configuration. This feature was surprising considering the reported information that suggested only the racemic polymer was able to crystallize. All polymers were thermally stable and showed a dependence of the maximum degradation rate temperature (from 242 °C to 342 °C) with the type of terpene oxide. The graduation of glass transition temperatures (from 44 °C to 172 °C) was also observed, being higher than those corresponding to the unsubstituted polymers. The chain stiffness of the studied polymers hindered both hydrolytic and enzymatic degradation while a higher rate was detected when an oxidative medium was assayed (e.g., weight losses around 12% after 21 days of exposure). All samples were biocompatible according to the adhesion and proliferation tests performed with fibroblast cells. Hydrophobic and mechanically consistent films (i.e., contact angles between 90° and 110°) were obtained after the evaporation of chloroform from the solutions, having different ratios of the studied biobased polyterpenes and poly(butylene succinate) (PBS). The blend films were comparable in tensile modulus and tensile strength with the pure PBS (e.g., values of 330 MPa and 7 MPa were determined for samples incorporating 30 wt.% of poly(PA-LO), the copolyester derived from limonene oxide and phthalic anhydride. Blends were degradable, biocompatible and appropriate to produce oriented-pore and random-pore scaffolds via a thermally-induced phase separation (TIPS) method and using 1,4-dioxane as solvent. The best results were attained with the blend composed of 70 wt.% PBS and 30 wt.% poly(PA-LO). In summary, the studied biobased terpene derivatives showed promising properties to be used in a blended form for biomedical applications such as scaffolds for tissue engineering

Medicated Scaffolds Prepared with Hydroxyapatite/Streptomycin Nanoparticles Encapsulated into Polylactide Microfibers

The preparation, characterization, and controlled release of hydroxyapatite (HAp) nanopar-ticles loaded with streptomycin (STR) was studied. These nanoparticles are highly appropriate for the treatment of bacterial infections and are also promising for the treatment of cancer cells. The analyses involved scanning electron microscopy, dynamic light scattering (DLS) and Z-potential measurements, as well as infrared spectroscopy and X-ray diffraction. Both amorphous (ACP) and crystalline (cHAp) hydroxyapatite nanoparticles were considered since they differ in their release behavior (faster and slower for amorphous and crystalline particles, respectively). The encapsulated nanoparticles were finally incorporated into biodegradable and biocompatible polylactide (PLA) scaf-folds. The STR load was carried out following different pathways during the synthesis/precipitation of the nanoparticles (i.e., nucleation steps) and also by simple adsorption once the nanoparticles were formed. The loaded nanoparticles were biocompatible according to the study of the cytotoxicity of extracts using different cell lines. FTIR microspectroscopy was also employed to evaluate the cytotoxic effect on cancer cell lines of nanoparticles internalized by endocytosis. The results were promising when amorphous nanoparticles were employed. The nanoparticles loaded with STR increased their size and changed their superficial negative charge to positive. The nanoparticles’ crystallinity decreased, with the consequence that their crystal sizes reduced, when STR was incorporated into their structure. STR maintained its antibacterial activity, although it was reduced during the adsorption into the nanoparticles formed. The STR release was faster from the amorphous ACP nanoparticles and slower from the crystalline cHAp nanoparticles. However, in both cases, the STR release was slower when incorporated in calcium and phosphate during the synthesis. The biocompatibility of these nanoparticles was assayed by two approximations. When extracts from the nanoparticles were evaluated in cultures of cell lines, no cytotoxic damage was observed at concen-trations of less than 10 mg/mL. This demonstrated their biocompatibility. Another experiment using FTIR microspectroscopy evaluated the cytotoxic effect of nanoparticles internalized by endocytosis in cancer cells. The results demonstrated slight damage to the biomacromolecules when the cells were treated with ACP nanoparticles. Both ACP and cHAp nanoparticles were efficiently encapsulated in PLA electrospun matrices, providing functionality and bioactive properties. © 2022 by the authors. Licensee MDPI, Basel, Switzerland

Key challenges, drivers and solutions for mobility management in 5G networks: a survey

Ensuring a seamless connection during the mobility of various User Equipments (UEs) will be one of the major challenges facing the practical implementation of the Fifth Generation (5G) networks and beyond. Several key determinants will significantly contribute to numerous mobility challenges. One of the most important determinants is the use of millimeter waves (mm-waves) as it is characterized by high path loss. The inclusion of various types of small coverage Base Stations (BSs), such as Picocell, Femtocell and drone-based BSs is another challenge. Other issues include the use of Dual Connectivity (DC), Carrier Aggregation (CA), the massive growth of mobiles connections, network diversity, the emergence of connected drones (as BS or UE), ultra-dense network, inefficient optimization processes, central optimization operations, partial optimization, complex relation in optimization operations, and the use of inefficient handover decision algorithms. The relationship between these processes and diverse wireless technologies can cause growing concerns in relation to handover associated with mobility. The risk becomes critical with high mobility speed scenarios. Therefore, mobility issues and their determinants must be efficiently addressed. This paper aims to provide an overview of mobility management in 5G networks. The work examines key factors that will significantly contribute to the increase of mobility issues. Furthermore, the innovative, advanced, efficient, and smart handover techniques that have been introduced in 5G networks are discussed. The study also highlights the main challenges facing UEs' mobility as well as future research directions on mobility management in 5G networks and beyond

Handover parameters optimisation techniques in 5G networks

The massive growth of mobile users will spread to significant numbers of small cells for the Fifth Generation (5G) mobile network, which will overlap the fourth generation (4G) network. A tremendous increase in handover (HO) scenarios and HO rates will occur. Ensuring stable and reliable connection through the mobility of user equipment (UE) will become a major problem in future mobile networks. This problem will be magnified with the use of suboptimal handover control parameter (HCP) settings, which can be configured manually or automatically. Therefore, the aim of this study is to investigate the impact of different HCP settings on the performance of 5G network. Several system scenarios are proposed and investigated based on different HCP settings and mobile speed scenarios. The different mobile speeds are expected to demonstrate the influence of many proposed system scenarios on 5G network execution. We conducted simulations utilizing MATLAB software and its related tools. Evaluation comparisons were performed in terms of handover probability (HOP), ping-pong handover probability (PPHP) and outage probability (OP). The 5G network framework has been employed to evaluate the proposed system scenarios used. The simulation results reveal that there is a trade-off in the results obtained from various systems. The use of lower HCP settings provides noticeable enhancements compared to higher HCP settings in terms of OP. Simultaneously, the use of lower HCP settings provides noticeable drawbacks compared to higher HCP settings in terms of high PPHP for all scenarios of mobile speed. The simulation results show that medium HCP settings may be the acceptable solution if one of these systems is applied. This study emphasises the application of automatic self-optimisation (ASO) functions as the best solution that considers user experience

Remote work arrangement: An investigation on the influence of team’s innovative performance in multinational NGOs in Jordan

As the workforce worldwide goes through a transformative shift towards remote work, this paper discusses the positive effects of this quite flexible work arrangement on team’s innovation performance (TIP) in multinational, non-governmental organizations (NGOs). Adopting cross-sectional, quantitative research design, empirical data were collected through a survey of 268 employees of multinational NGOs operating in Jordan. The collected data were, then, analyzed using structural equation modeling. The results of the analysis showed that remote work has significant, positive effects on TIP in NGOs. Of the various remote work features investigated, spatial flexibility has the highest effect. The study results contribute to the ongoing discourse on the future of the work styles and have implications for leaders, policymakers, and practitioners who seek promoting innovation in multinational NGOs