System Analysis Applied to Autonomy: Application to High-Altitude Long-Endurance Remotely Operated Aircraft

Maturation of intelligent systems technologies and their incorporation into aerial platforms are dictating the development of new analysis tools and incorporation of such tools into existing system analysis methodologies in order to fully capture the trade-offs of autonomy on vehicle and mission success. A first-order "system analysis of autonomy" methodology is outlined in this paper. Further, this analysis methodology is subsequently applied to notional high-altitude long-endurance (HALE) aerial vehicle missions

Approaching Mechanization of Plant Micropropagation

Investigations of materials and methods for growing plant tissue in a continual-flow, liquid nutrient medium as an alternative to semisolid agar medium have been made. Enhanced growth of plant tissue on microporouspolypropylene membranes floating on a liquid nutrient has been demonstrated. Moreover, in vitro plantlets on the microporous membrane are free from entanglement with the support matrix and readily available to mechanizedhandling. Trained growth of plantlets through polypropylene netting shows potential for mechanization by mass handling (separation, singulation, and transfer) of plant tissue cultures

On the Classification of UGC1382 as a Giant Low Surface Brightness Galaxy

We provide evidence that UGC1382, long believed to be a passive elliptical galaxy, is actually a giant low surface brightness (GLSB) galaxy which rivals the archetypical GLSB Malin 1 in size. Like other GLSB galaxies, it has two components: a high surface brightness disk galaxy surrounded by an extended low surface brightness (LSB) disk. For UGC1382, the central component is a lenticular system with an effective radius of 6 kpc. Beyond this, the LSB disk has an effective radius of ~38 kpc and an extrapolated central surface brightness of ~26 mag/arcsec^2. Both components have a combined stellar mass of ~8x10^10 M_sun, and are embedded in a massive (10^10 M_sun) low-density (<3 M_sun/pc^2) HI disk with a radius of 110 kpc, making this one of the largest isolated disk galaxies known. The system resides in a massive dark matter halo of at least 2x10^12 M_sun. Although possibly part of a small group, its low density environment likely plays a role in the formation and retention of the giant LSB and HI disks. We model the spectral energy distributions and find that the LSB disk is likely older than the lenticular component. UGC1382 has UV-optical colors typical of galaxies transitioning through the green valley. Within the LSB disk are spiral arms forming stars at extremely low efficiencies. The gas depletion time scale of ~10^11 yr suggests that UGC1382 may be a very long term resident of the green valley. We find that the formation and evolution of the LSB disk is best explained by the accretion of gas-rich LSB dwarf galaxies.Comment: 17 pages, 16 figures, 4 tables; accepted to the Astrophysical Journa

Feasibility of using Grindr™ to distribute HIV self-test kits to men who have sex with men in Los Angeles, California

Background: Our study aimed to determine if Grindr™ is an effective means of reaching high-risk men who have sex with men (MSM) for HIV testing. In Los Angeles (LA), Black and Latino MSM have the highest rate of HIV infection, and Black MSM in LA are four-fold more likely than white MSM to not know they are infected with HIV. Those MSM are also major users of social networking apps. Grindr™ was used to provide access to free HIV self-testing. Methods: Free HIV self-test kits were advertised on Grindr™ from 13 October to 11 November 2014, consisting of 300 000 banner ads and three broadcast messages targeting a high-risk HIV population in LA. Eligible participants, Black or Latino, MSM and who were aged ≥18 years of age, were invited to take a survey 2 weeks after test delivery. Results: The website received 4389 unique visitors and 333 test requests, of which 247 (74%) were requests for mailed tests, 58 (17%) were for vouchers and 28 (8%) were for vending machines. Of the 125 participants, 74% reported at least one episode of condomless anal intercourse in the past 3 months, 29% last tested for HIV over 1 year ago and 9% had never been tested. Conclusions: It was feasible to use Grindr™ to distribute HIV self-test kits. Users are willing to provide personal information in exchange for a free self-test and found self-tests acceptable and easy to use. HIV self-testing promotion through apps has a high potential to reach untested high-risk populations

Multiple major increases and decreases in mitochondrial substitution rates in the plant family Geraniaceae

Background: Rates of synonymous nucleotide substitutions are, in general, exceptionally low in plant mitochondrial genomes, several times lower than in chloroplast genomes, 10-20 times lower than in plant nuclear genomes, and 50-100 times lower than in many animal mitochondrial genomes. Several cases of moderate variation in mitochondrial substitution rates have been reported in plants, but these mostly involve correlated changes in chloroplast and/or nuclear substitution rates and are therefore thought to reflect whole-organism forces rather than ones impinging directly on the mitochondrial mutation rate. Only a single case of extensive, mitochondrial-specific rate changes has been described, in the angiosperm genus Plantago. Results: We explored a second potential case of highly accelerated mitochondrial sequence evolution in plants. This case was first suggested by relatively poor hybridization of mitochondrial gene probes to DNA of Pelargonium hortorum (the common geranium). We found that all eight mitochondrial genes sequenced from P. hortorum are exceptionally divergent, whereas chloroplast and nuclear divergence is unexceptional in P. hortorum. Two mitochondrial genes were sequenced from a broad range of taxa of variable relatedness to P. hortorum, and absolute rates of mitochondrial synonymous substitutions were calculated on each branch of a phylogenetic tree of these taxa. We infer one major, similar to 10-fold increase in the mitochondrial synonymous substitution rate at the base of the Pelargonium family Geraniaceae, and a subsequent similar to 10-fold rate increase early in the evolution of Pelargonium. We also infer several moderate to major rate decreases following these initial rate increases, such that the mitochondrial substitution rate has returned to normally low levels in many members of the Geraniaceae. Finally, we find unusually little RNA editing of Geraniaceae mitochondrial genes, suggesting high levels of retroprocessing in their history. Conclusion: The existence of major, mitochondrial-specific changes in rates of synonymous substitutions in the Geraniaceae implies major and reversible underlying changes in the mitochondrial mutation rate in this family. Together with the recent report of a similar pattern of rate heterogeneity in Plantago, these findings indicate that the mitochondrial mutation rate is a more plastic character in plants than previously realized. Many molecular factors could be responsible for these dramatic changes in the mitochondrial mutation rate, including nuclear gene mutations affecting the fidelity and efficacy of mitochondrial DNA replication and/or repair and consistent with the lack of RNA editing - exceptionally high levels of mutagenic retroprocessing. That the mitochondrial mutation rate has returned to normally low levels in many Geraniaceae raises the possibility that, akin to the ephemerality of mutator strains in bacteria, selection favors a low mutation rate in plant mitochondria

Increased risk for other cancers in individuals with Ewing sarcoma and their relatives.

BackgroundThere are few reports of the association of other cancers with Ewing sarcoma in patients and their relatives. We use a resource combining statewide genealogy and cancer reporting to provide unbiased risks.MethodsUsing a combined genealogy of 2.3 million Utah individuals and the Utah Cancer Registry (UCR), relative risks (RRs) for cancers of other sites were estimated in 143 Ewing sarcoma patients using a Cox proportional hazards model with matched controls; however, risks in relatives were estimated using internal cohort-specific cancer rates in first-, second-, and third-degree relatives.ResultsCancers of three sites (breast, brain, complex genotype/karyotype sarcoma) were observed in excess in Ewing sarcoma patients. No Ewing sarcoma patients were identified among first-, second-, or third-degree relatives of Ewing sarcoma patients. Significantly increased risk for brain, lung/bronchus, female genital, and prostate cancer was observed in first-degree relatives. Significantly increased risks were observed in second-degree relatives for breast cancer, nonmelanoma eye cancer, malignant peripheral nerve sheath cancer, non-Hodgkin lymphoma, and translocation sarcomas. Significantly increased risks for stomach cancer, prostate cancer, and acute lymphocytic leukemia were observed in third-degree relatives.ConclusionsThis analysis of risk for cancer among Ewing sarcoma patients and their relatives indicates evidence for some increased cancer predisposition in this population which can be used to individualize consideration of potential treatment of patients and screening of patients and relatives

Is Body Composition or Body Mass Index Associated with the Step Count Accuracy of a Wearable Technology Device?

Topics in Exercise Science and Kinesiology Volume 3: Issue 1, Article 5, 2022. A simple way to gauge daily physical activity levels is to use a wearable technology device to count the number of steps taken during the day. However, it is unknown whether these devices return accurate step counts for persons with different body fat percentages or body mass index scores. The purpose was to determine if there is a correlation between either body fat percentages and/or body mass index values and the percent error calculated between a manual step count and values recorded by a wearable technology device. Forty volunteers participated. The Samsung Gear 2, FitBit Surge, Polar A360, Garmin Vivosmart HR+, and the Leaf Health Tracker were evaluated when walking and jogging in free motion and treadmill conditions. All devices were worn simultaneously in randomized configurations. The mean of two manual steps counters was used as the criterion measure. Walking and jogging free motion and treadmill protocols of 5-minute intervals were completed. Correlation was determined by Spearman’s rank correlation coefficient. Significance was set at \u3c0.05. There were no significant correlations for body mass index vs percent error. For body fat, significant positive correlations were observed for the Samsung Gear 2 free motion walk: (r=0.321, p=0.043), Garmin Vivosmart HR+ free motion walk: (r=0.488, p=\u3c0.001), and the Leaf Health Tracker treadmill walk: (r=0.368, p=0.020) and treadmill jog: (r=0.350, p=0.027). Body fat may have a limited association with a device’s step count percent error. Lower body mechanics along with device placement may be more of a factor in step counting accuracy

The “fossilized” mitochondrial genome of Liriodendron tulipifera: ancestral gene content and order, ancestral editing sites, and extraordinarily low mutation rate

BACKGROUND: The mitochondrial genomes of flowering plants vary greatly in size, gene content, gene order, mutation rate and level of RNA editing. However, the narrow phylogenetic breadth of available genomic data has limited our ability to reconstruct these traits in the ancestral flowering plant and, therefore, to infer subsequent patterns of evolution across angiosperms. RESULTS: We sequenced the mitochondrial genome of Liriodendron tulipifera, the first from outside the monocots or eudicots. This 553,721 bp mitochondrial genome has evolved remarkably slowly in virtually all respects, with an extraordinarily low genome-wide silent substitution rate, retention of genes frequently lost in other angiosperm lineages, and conservation of ancestral gene clusters. The mitochondrial protein genes in Liriodendron are the most heavily edited of any angiosperm characterized to date. Most of these sites are also edited in various other lineages, which allowed us to polarize losses of editing sites in other parts of the angiosperm phylogeny. Finally, we added comprehensive gene sequence data for two other magnoliids, Magnolia stellata and the more distantly related Calycanthus floridus, to measure rates of sequence evolution in Liriodendron with greater accuracy. The Magnolia genome has evolved at an even lower rate, revealing a roughly 5,000-fold range of synonymous-site divergence among angiosperms whose mitochondrial gene space has been comprehensively sequenced. CONCLUSIONS: Using Liriodendron as a guide, we estimate that the ancestral flowering plant mitochondrial genome contained 41 protein genes, 14 tRNA genes of mitochondrial origin, as many as 7 tRNA genes of chloroplast origin, >700 sites of RNA editing, and some 14 colinear gene clusters. Many of these gene clusters, genes and RNA editing sites have been variously lost in different lineages over the course of the ensuing ∽200 million years of angiosperm evolution

Theoretical Evaluation of Anisotropic Reflectance Correction Approaches for Addressing Multi-Scale Topographic Effects on the Radiation-Transfer Cascade in Mountain Environments

Research involving anisotropic-reflectance correction (ARC) of multispectral imagery to account for topographic effects has been ongoing for approximately 40 years. A large body of research has focused on evaluating empirical ARC methods, resulting in inconsistent results. Consequently, our research objective was to evaluate commonly used ARC methods using first-order radiation-transfer modeling to simulate ASTER multispectral imagery over Nanga Parbat, Himalaya. Specifically, we accounted for orbital dynamics, atmospheric absorption and scattering, direct- and diffuse-skylight irradiance, land cover structure, and surface biophysical variations to evaluate their effectiveness in reducing multi-scale topographic effects. Our results clearly reveal that the empirical methods we evaluated could not reasonably account for multi-scale topographic effects at Nanga Parbat. The magnitude of reflectance and the correlation structure of biophysical properties were not preserved in the topographically-corrected multispectral imagery. The CCOR and SCS+C methods were able to remove topographic effects, given the Lambertian assumption, although atmospheric correction was required, and we did not account for other primary and secondary topographic effects that are thought to significantly influence spectral variation in imagery acquired over mountains. Evaluation of structural-similarity index images revealed spatially variable results that are wavelength dependent. Collectively, our simulation and evaluation procedures strongly suggest that empirical ARC methods have significant limitations for addressing anisotropic reflectance caused by multi-scale topographic effects. Results indicate that atmospheric correction is essential, and most methods failed to adequately produce the appropriate magnitude and spatial variation of surface reflectance in corrected imagery. Results were also wavelength dependent, as topographic effects influence radiation-transfer components differently in different regions of the electromagnetic spectrum. Our results explain inconsistencies described in the literature, and indicate that numerical modeling efforts are required to better account for multi-scale topographic effects in various radiation-transfer components.Open access journalThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]