A rat model for the immune response to the intrauterine administration of BCG

This study was designed to investigate the changes in the numbers of lymphocytes, macrophages and plasma cells in the uterus and ileocecal lymph nodes of rats exposed to the intrauterine administration of Baeillus-Calmette Guerin (BCG). Thirty female Wistar Albino rats, age 6 months and weighing between 200-250 g, were assigned to the two experimental groups BCG treated and controls (n = 15). The intrauterine BCG injections were made using laparatomy in the diestrous cycle under Rompun and Ketalar anesthesia. 0.1 ml BCG were injected for each into cornu uteri while the control group received 0.1 ml sterile saline in the same place. Two weeks later, the rats in both groups were anesthetized with ether and decapitated. Uterus and ileocecal lymph nodes were processed to determine alpha naphthyl acid esterase (ANAE) - positive T lymphocytes and macrophages. The plasma cells were stained with the methyl green-pyronin method; It was found that the numbers of lymphocytes macrophages and plasma cells on the uterus increased (P<0.01) in BCG treated rats. In addition, the number of these cells also increased in the ileoeeeal lymph nodes indicating the presence of an immune response to the intrauterine BCG administration, It is concluded that although the rat was chosen as a model and ECG was given by the process of laparatomy in this study, intracervical administration of BCG tn the uterus should be studied clinically in cases of immune deficiency disorders related to the uterus. such as endometritis, myometritis, pyometra, endometriosis. infertility and implantation problems of domestic animals, to see if there is an increase in the immune response

Antitumor effect of sFlt-1 gene therapy system mediated by Bifidobacterium Infantis on Lewis lung cancer in mice

Soluble fms-like tyrosine kinase receptor (sFlt-1) is a soluble form of extramembrane part of vascular endothelial growth factor receptor-1 (VEGFR-1) that has antitumor effects. Bifidobacterium Infantis is a kind of non-pathogenic and anaerobic bacteria that may have specific targeting property of hypoxic environment inside of solid tumors. The aim of this study was to construct Bifidobacterium Infantis-mediated sFlt-1 gene transferring system and investigate its antitumor effect on Lewis lung cancer (LLC) in mice. Our results demonstrated that the Bifidobacterium Infantis-mediated sFlt-1 gene transferring system was constructed successfully and the system could express sFlt-1 at the levels of gene and protein. This system could not only significantly inhibit growth of human umbilical vein endothelial cells induced by VEGF in vitro, but also inhibit the tumor growth and prolong survival time of LLC C57BL/6 mice safely. These data suggest that Bifidobacterium Infantis-mediated sFlt-1 gene transferring system presents a promising therapeutic approach for the treatment of cancer

A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

Meeting abstrac

Laparoscopic versus open cholecystectomy: effect on pulmonary function tests

To compare the changes in pulmonary function tests (PFT) after open and laparoscopic cholecystectomy, two groups of 25 patients were set up. Each patient had 3 PFTs performed and 2 PA grid chest roentgenograms taken. After taken into consideration the difference between PFT values were not significant (p less than or equal to 0.05). All PFT values decreased significantly on the first postoperative day (p less than or equal to 0.05). When PA chess roentgenograms were compared no clinically evident atelectasis except 1 lineary was seen in laparoscopic cholecystectomy group whereas 3 lineary, 4 focal, and 1 segmentary atelectasia were encountered in open cholecystectomy group (p less than or equal to 0.05). We believe that laparoscopic cholecystectomy has more advantages when speaking of postoperative PFT and atelectasia

Nasopharyngeal carcinoma in childhood: long-term results of 32 patients

Purpose: To evaluate treatment results and prognostic factors, pediatric patients with nasopharyngeal carcinoma were reviewed

Laparoscopic versus open cholecystectomy: Effect on pulmonary function tests

Background/Aims: Operations often cause impairment in respiration due to pain. This study was designed to compare the changes in pulmonary function tests after open and laparoscopic cholecystectomy