Changes in murine anorectum signaling across the life course

Background: Increasing age is associated with an increase in the incidence of chronic constipation and fecal impaction. The contribution of the natural aging process to these conditions is not fully understood. This study examined the effects of increasing age on the function of the murine anorectum.Methods: The effects of increasing age on cholinergic, nitrergic, and purinergic signaling pathways in the murine anorectum were examined using classical organ bath assays to examine tissue function and electrochemical sensing to determine age‐related changes in nitric oxide and acetylcholine release.Key Results: Nitrergic relaxation increased between 3 and 6 months, peaked at 12 months and declined in the 18 and 24 months groups. These changes were in part explained by an age‐related decrease in nitric oxide (NO) release. Cholinergic signaling was maintained with age by an increase in acetylcholine (ACh) release and a compensatory decrease in cholinesterase activity. Age‐related changes in purinergic relaxation were qualitatively similar to nitrergic relaxation although the relaxations were much smaller. Increasing age did not alter the response of the anorectum smooth muscle to exogenously applied ACh, ATP, sodium nitroprusside or KCl. Similarly, there was no change in basal tension developed by the anorectum.Conclusions and Inferences: The decrease in nitrergic signaling with increasing age may contribute to the age‐related fecal impaction and constipation previously described in this model by partially obstructing defecation

Interstitial cell network volume is reduced in the terminal bowel of ageing mice

Ageing is associated with impaired neuromuscular function of the terminal gastrointestinal (GI) tract, which can result in chronic constipation, faecal impaction and incontinence. Interstitial cells of cajal (ICC) play an important role in regulation of intestinal smooth muscle contraction. However, changes in ICC volume with age in the terminal GI tract (the anal canal including the anal sphincter region and rectum)have not been studied. Here, the distribution, morphology and network volume of ICC in the terminal GI tract of 3‐to 4‐month‐old and 26‐to 28‐month‐old C57BL/6mice were investigated. ICC were identified by immunofluorescence labelling of wholemount preparations with an antibody against c‐Kit. ICC network volume was measured by software‐based 3D volume rendering of confocal Z stacks. A significant reduction in ICC network volume per unit volume of muscle was measured in aged animals. No age‐associated change in ICC morphology was detected. The thickness of the circular muscle layer of the anal sphincter region and rectum increased with age, while that in the distal colon decreased. These results suggest that ageing is associated with a reduction in the network volume of ICC in the terminal GI tract, which may influence the normal function of these regions

Cluster spacecraft observations of a ULF wave enhanced by Space Plasma Exploration by Active Radar (SPEAR)

Space Plasma Exploration by Active Radar (SPEAR) is a high-latitude ionospheric heating facility capable of exciting ULF waves on local magnetic field lines. We examine an interval from 1 February 2006 when SPEAR was transmitting a 1 Hz modulation signal with a 10 min on-off cycle. Ground magnetometer data indicated that SPEAR modulated currents in the local ionosphere at 1 Hz, and enhanced a natural field line resonance with a 10 min period. During this interval the Cluster spacecraft passed over the heater site. Signatures of the SPEAR-enhanced field line resonance were present in the magnetic field data measured by the magnetometer on-board Cluster-2. These are the first joint ground- and space-based detections of field line tagging by SPEAR

A comparison of velocity measurements from the CUTLASS Finland radar and the EISCAT UHF system

International audienceThe CUTLASS Finland radar, which comprises an integral part of the SuperDARN system of HF coherent radars, provides near continuous observations of high-latitude plasma irregularities within a field-of-view which extends over some four million square kilometres. Within the Finland radar field-of-view lie both the EISCAT mainland and EISCAT Svalbard incoherent scatter radar facilities. Since the CUTLASS Finland radar commenced operation, in February 1995, the mainland EISCAT UHF radar has been run in common programme 1 and 2 modes for a total duration exceeding 1000 h. Simultaneous and spatially coincident returns from these two radars over this period provide the basis for a comparison of irregularity drift velocity and F-region ion velocity. Initial comparison is limited to velocities from four intervals of simultaneous radar returns; intervals are selected such that they exhibit a variety of velocity signatures including that characteristic of the convection reversal and a rapidly fluctuating velocity feature. Subsequent comparison is on a statistical basis. The velocities measured by the two systems demonstrate reasonable correspondence over the velocity regime encountered during the simultaneous occurrence of coherent and incoherent scatter; differences between the EISCAT UHF measurements of F-region ion drift and the irregularity drift velocities from the Finland radar are explained in terms of a number of contributing factors including contamination of the latter by E-region echoes, a factor which is investigated further, and the potentially deleterious effect of discrepant volume and time sampling intervals

Testing nowcasts of the ionospheric convection from the expanding and contracting polar cap model

The expanding/contracting polar cap (ECPC) model, or the time-dependent Dungey cycle, provides a theoretical framework for understanding solar wind-magnetosphere-ionosphere coupling. The ECPC describes the relationship between magnetopause reconnection and substorm growth phase, magnetotail reconnection and substorm expansion phase, associated changes in auroral morphology, and ionospheric convective motions. Despite the many successes of the model, there has yet to be a rigorous test of the predictions or nowcasts made regarding ionospheric convection, which remains a final hurdle for the validation of the ECPC. In this study we undertake a comparison of ionospheric convection, as measured in situ by ion drift meters on board DMSP (Defense Meteorological Satellite Program) satellites and from the ground by SuperDARN (Super Dual Auroral Radar Network), with motions nowcasted by a theoretical model. The model is coupled to measurements of changes in the size of the polar cap made using global auroral imagery from the IMAGE FUV (Imager for Magnetopause to Aurora Global Exploration Far Ultraviolet) instrument, as well as the dayside reconnection rate, estimated using the OMNI data set. The results show that we can largely nowcast the magnitudes of ionospheric convection flows using the context of our understanding of magnetic reconnection at the magnetopause and in the magnetotail

First simultaneous observations of flux transfer events at the high-latitude magnetopause by the cluster spacecraft and pulsed radar signatures in the conjugate ionosphere by the CUTLASS and EISCAT radars

Cluster magnetic field data are studied during an outbound pass through the post-noon high-latitude magnetopause region on 14 February 2001. The onset of several minute perturbations in the magnetospheric field was observed in conjunction with a southward turn of the interplanetary magnetic field observed upstream by the ACE spacecraft and lagged to the subsolar magnetopause. These perturbations culminated in the observation of four clear magnetospheric flux transfer events (FTEs) adjacent to the magnetopause, together with a highly-structured magnetopause boundary layer containing related field features. Furthermore, clear FTEs were observed later in the magnetosheath. The magnetospheric FTEs were of essentially the same form as the original “flux erosion events” observed in HEOS-2 data at a similar location and under similar interplanetary conditions by Haerendel et al. (1978). We show that the nature of the magnetic perturbations in these events is consistent with the formation of open flux tubes connected to the northern polar ionosphere via pulsed reconnection in the dusk sector magnetopause. The magnetic footprint of the Cluster spacecraft during the boundary passage is shown to map centrally within the fields-of-view of the CUTLASS SuperDARN radars, and to pass across the field-aligned beam of the EISCAT Svalbard radar (ESR) system. It is shown that both the ionospheric flow and the backscatter power in the CUTLASS data pulse are in synchrony with the magnetospheric FTEs and boundary layer structures at the latitude of the Cluster footprint. These flow and power features are subsequently found to propagate poleward, forming classic “pulsed ionospheric flow” and “poleward-moving radar auroral form” structures at higher latitudes. The combined Cluster-CUTLASS observations thus represent a direct demonstration of the coupling of momentum and energy into the magnetosphere-ionosphere system via pulsed magnetopause reconnection. The ESR observations also reveal the nature of the structured and variable polar ionosphere produced by the structured and time-varying precipitation and flow

Finite element investigation of the effect of spina bifida on loading of the vertebral isthmus

Background: Spondylolysis (SL) of the lower lumbar spine is frequently associated with spina bifida occulta (SBO). There has not been any study that has demonstrated biomechanical or genetic predispositions to explain the coexistence of these two pathologies. In axial rotation, the intact vertebral arch allows torsional load to be shared between the facet joints. In SBO, the load cannot be shared across the arch, theoretically increasing the mechanical demand of the vertebral isthmus during combined axial loading and rotation when compared to the normal state. Purpose: To test the hypothesis that fatigue failure limits will be exceeded in the case of a bifid arch, but not in the intact case, when the segment is subjected to complex loading corresponding to normal sporting activities. Study Design: Descriptive Laboratory Study. Methods: Finite element models of natural and SBO (L4-S1) including ligaments were loaded axially to 1kN and were combined with axial rotation of 3°. Bilateral stresses, alternating stresses and shear fatigue failure on intact and SBO L5 isthmus were assessed and compared. Results: Under 1kN axial load, the von Mises stresses observed in SBO and in the intact cases were very similar (differences <5MPa) having a maximum at the ventral end of the isthmus that decreases monotonically to the dorsal end. However, under 1kN axial load and rotation, the maximum von Mises stresses observed in the ipsilateral L5 isthmus in the SBO case (31MPa) was much higher than the intact case (24.2MPa) indicating a lack of load sharing across the vertebral arch in SBO. When assessing the equivalent alternating shear stress amplitude, this was found to be 22.6 MPa for the SBO case and 13.6 MPa for the intact case. From this it is estimated that shear fatigue failure will occur in less than 70,000 cycles, under repetitive axial load & rotation conditions in the SBO case, while for the intact case, fatigue failure will occur only over 10 million cycles. Conclusion: SBO predisposes spondylolysis by generating increased stresses across the inferior isthmus of the inferior articular process, specifically in combined axial rotation and anteroposterior shear. Clinical Relevance: Athletes with SBO who participate in sports that require repetitive lumbar rotation, hyperextension and/or axial loading are at a higher risk of developing spondylolysis compared to athletes with an intact spine