Software and Analysis for Dynamic Voronoi Diagrams in the Hilbert Metric

The Hilbert metric is a projective metric defined on a convex body which generalizes the Cayley-Klein model of hyperbolic geometry to any convex set. In this paper we analyze Hilbert Voronoi diagrams in the Dynamic setting. In addition we introduce dynamic visualization software for Voronoi diagrams in the Hilbert metric on user specified convex polygons

A rapid and reliable method for the determination of Lactiplantibacillus plantarum during wine fermentation based on PMA-CELL-qPCR

Real-time monitoring of microbial dynamics during fermentation is essential for wine quality control. This study developed a method that combines the fluorescent dye propidium monoazide (PMA) with CELL-qPCR, which can distinguish between dead and live microbes for Lactiplantibacillus plantarum. This method could detect the quantity of microbes efficiently and rapidly without DNA extraction during wine fermentation. The results showed that (1) the PMA-CELL-qPCR enumeration method developed for L. plantarum was optimized for PMA treatment concentration, PMA detection sensitivity and multiple conditions of sample pretreatment in wine environment, and the optimized method can accurately quantify 104–108 CFU/mL of the target strain (L. plantarum) in multiple matrices; (2) when the concentration of dead bacteria in the system is 104 times higher than the concentration of live bacteria, there is an error of 0.5–1 lg CFU/mL in the detection results. The optimized sample pretreatment method in wine can effectively reduce the inhibitory components in the qPCR reaction system; (3) the optimized PMA-CELL-qPCR method was used to monitor the dynamic changes of L. plantarum during the fermentation of Cabernet Sauvignon wine, and the results were consistent with the plate counting method. In conclusion, the live bacteria quantification method developed in this study for PMA-CELL-qPCR in L. plantarum wines is accurate in quantification and simple in operation, and can be used as a means to accurately monitor microbial dynamics in wine and other fruit wines

Social media sentiment of hydrogen fuel cell vehicles in China: Evidence from artificial intelligence algorithms

Hydrogen energy is significant in the energy consumption, especially in Hydrogen Fuel Cell Vehicles(HFCVs) market. Social media data is critical for exploring public perceptions of HFCVs. To find hot topics and understand the public sentiment of HFCVs, we employ a computational model, which combines Kmeans algorithm, Latent Dirichlet Allocation (LDA), and SnowNLP. The training data consists of 42,063 comments sourced from Bilibili-a popular Chinese social media platform. The analysis has identified 12 clusters, each with distinct topics and sentiments. The results reveal that the Chinese public generally holds a neutral stance on the hydrogen energy market, while some stakeholders maintain a positive on the technology and development of HFCVs, but some concerns about the transportation and safety of hydrogen fuel. Furthermore, this study offers suggestions for the technological, operational, and strategic advancement of HFCVs

DataSheet2_Cyclovirobuxine D, a cardiovascular drug from traditional Chinese medicine, alleviates inflammatory and neuropathic pain mainly via inhibition of voltage-gated Cav3.2 channels.docx

Cyclovirobuxine D (CVB-D), the main active constituent of traditional Chinese medicine Buxus microphylla, was developed as a safe and effective cardiovascular drug in China. B. microphylla has also been used to relieve various pain symptoms for centuries. In this study, we examined and uncovered strong and persistent analgesic effects of cyclovirobuxine D against several mouse models of pain, including carrageenan- and CFA-induced inflammatory pain and paclitaxel-mediated neuropathic hypersensitivity. Cyclovirobuxine D shows comparable analgesic effects by intraplantar or intraperitoneal administration. Cyclovirobuxine D potently inhibits voltage-gated Cav2.2 and Cav3.2 channels but has negligible effects on a diverse group of nociceptive ion channels distributed in primary afferent neurons, including Nav1.7, Nav1.8, TRPV1, TPRA1, TRPM8, ASIC3, P2X2 and P2X4. Moreover, inhibition of Cav3.2, rather than Cav2.2, plays a dominant role in attenuating the excitability of isolated dorsal root ganglion neurons and pain relieving effects of cyclovirobuxine D. Our work reveals that a currently in-use cardiovascular drug has strong analgesic effects mainly via blockade of Cav3.2 and provides a compelling rationale and foundation for conducting clinical studies to repurpose cyclovirobuxine D in pain management.</p

Free-Standing NiO-MgO-Al₂O₃ Nanosheets Derived from Layered Double Hydroxides Grown onto FeCrAl-Fiber as Structured Catalysts for Dry Reforming of Methane

An FeCrAl-fiber-structured NiO-MgO-Al₂O₃ nanocomposite catalyst engineered from nano- to macro-scale in one-step is developed via thermally decomposing NiMgAl layered double hydroxides (LDHs) that can be grown onto the FeCrAl-fiber only through a γ-Al₂O₃/water interface-assisted method. By taking advantages of homogeneous component-distribution in the LDHs-derived NiO-MgO-Al₂O₃ nanocomposites and enhanced heat transfer, this promising catalyst delivers satisfying performance with enhanced coking/sintering resistance in the title reaction. At 800 °C and a gas hourly space velocity of 5000 mL g^–1 h^–1, CH₄/CO₂ conversion maintains almost constant at 91%/89% within the initial 90 h and then slides in a smooth downturn (to 80/85%) within another 180 h of reaction

Data_Sheet_1_A rapid and reliable method for the determination of Lactiplantibacillus plantarum during wine fermentation based on PMA-CELL-qPCR.docx

Real-time monitoring of microbial dynamics during fermentation is essential for wine quality control. This study developed a method that combines the fluorescent dye propidium monoazide (PMA) with CELL-qPCR, which can distinguish between dead and live microbes for Lactiplantibacillus plantarum. This method could detect the quantity of microbes efficiently and rapidly without DNA extraction during wine fermentation. The results showed that (1) the PMA-CELL-qPCR enumeration method developed for L. plantarum was optimized for PMA treatment concentration, PMA detection sensitivity and multiple conditions of sample pretreatment in wine environment, and the optimized method can accurately quantify 104–108 CFU/mL of the target strain (L. plantarum) in multiple matrices; (2) when the concentration of dead bacteria in the system is 104 times higher than the concentration of live bacteria, there is an error of 0.5–1 lg CFU/mL in the detection results. The optimized sample pretreatment method in wine can effectively reduce the inhibitory components in the qPCR reaction system; (3) the optimized PMA-CELL-qPCR method was used to monitor the dynamic changes of L. plantarum during the fermentation of Cabernet Sauvignon wine, and the results were consistent with the plate counting method. In conclusion, the live bacteria quantification method developed in this study for PMA-CELL-qPCR in L. plantarum wines is accurate in quantification and simple in operation, and can be used as a means to accurately monitor microbial dynamics in wine and other fruit wines.</p

Microfibrous-Structured SS-fiber@meso-HZSM‑5 Catalyst for Methanol-to-Propylene: Steam-Assisted Crystallization Synthesis and Insight into the Stability Enhancement

Free-standing stainless-steel (SS)-fiber@meso-HZSM-5 core–shell catalysts engineered from micro- to macro-scale were highly efficiently synthesized via cost-effective steam-assisted crystallization (SAC) method. Impact of synthesis conditions on their morphology and textural/acidic properties was investigated by means of XRD, SEM, TEM, solid-state NMR, NH₃-TPD, Py-IR and N₂ physisorption. Single-run lifetime of such structured catalysts for MTP process was strongly dependent on their preparation conditions but slightly the product distribution. A volcano-like relationship for lifetime was observed against the SAC time, which was correlated well with the crystallization-time-dependent crystallinity, mesoporosity and crystal size. The promising SS-fiber@meso-HZSM-5 was the one obtained after the SAC for 12 h, which delivered a prolonged single-run lifetime of 620 h with a high propylene selectivity of ∼42% at 450 °C using a methanol weight hourly space velocity (WHSV) of 1 h^–1, as the result of high crystallinity, well-developed mesoporosity and small crystal size. Note that well-developed mesoporosity was paramount for the catalyst stability improvement, because of enhanced accommodation capacity of zeolite shell for receiving formed coke

Clinical Significance of CD147 in Children with Inflammatory Bowel Disease

Background. CD147/basigin (Bsg), a transmembrane glycoprotein, activates matrix metalloproteinases and promotes inflammation. Objective. The aim of this study is to explore the clinical significance of CD147 in the pathogenesis of inflammatory bowel disease (IBD). Results. In addition to monocytes, the clinical analysis showed that there is no significance obtained in leucocyte, neutrophil, eosinophil, basophil, and erythrocyte between IBD and controls. Immunohistochemistry analysis showed that CD147 was increased in intestinal tissue of patients with active IBD compared to that in the control group. What is more, CD147 is involved in intestinal barrier function and intestinal inflammation, which was attributed to the fact that it has an influence on MCT4 expression, a regulator of intestinal barrier function and intestinal inflammation, in HT-29 and CaCO2 cells. Most importantly, serum level of CD147 content is higher in active IBD than that in inactive IBD or healthy control, which could be a biomarker of IBD. Conclusion. The data suggested that increased CD147 level could be a biomarker of IBD in children