Bioavailability of metal(loid)s from micro- and nanometric particles in relation with their phytotoxicity

Ultrafine particles including nanosized enriched with metal(loid)s (PM) are emitted into the atmosphere of industrial or urban areas, these PM can transfer into soil and water ecosystems and have consequences on plant quality and human health. In a global socio-scientific context that regulation on (eco)toxicity of chemicals and public space pressures are recently increased, studies of environmental and health impacts throughout the life cycle of PM are of crucial sanitary concern. The PhD aims first to study metal(loid)s present in the PM: their transfer kinetic and mechanism of phytoavailability, phytotoxicity, and human health risks-ingestion bioaccessibility. Then, through the case of vegetable gardens near an incinerator and a highway in China, a socio-scientific study was performed in order to give suggestions for sustainable environmental and health risk management for these sites. Vegetables can significantly accumulate metal(loid)s by foliar uptake when PM directly enter into leaves through stomata apertures. Ultrafine PbO and nano-CuO particles caused serious phytotoxicity (reduced biomass and gaseous exchange, and necrosis) after interaction with leaf surface. Phytotoxicity of metal(loid)s is not simply governed by their total concentration, but also depended on the potential bio-transformation. Electron paramagnetic resonance (EPR) analysis clearly evidenced copper speciation change in leaf tissues. Moreover, a significant influence of the nature of metal, plant species and the exposure pathways (foliar/root) on gastro-bioaccessibility of metal(loid)s had been demonstrated. For a social-scientific study near waste incinerator and roadside, we found that atmosphere PM fallouts can induce significant metal foliar uptake in addition to soil-plant transfer. The relatively high human bioaccessibility of metal (60-79%) was measured, suggesting a potential health risk in the case of regular consumption of polluted vegetables. Vegetable gardens present a low (waste incinerator) or moderate (highway) health risk with respect to human consumption quantity of the investigated vegetables, but exposure to different organic pollutants in addition to metals is often possible. Our studies highlight the importance of taking atmosphere and soil quality into account for estimating the quality of consumed plants grown in anthropic areas (farms and kitchen gardens), and for sustainable management of urban agricultures

Agriculture urbaine durable : opportunité pour la transition écologique

Selon la Food and Agriculture Organization, 80 % des humains seront urbains en 2050. Le développement de l’agriculture urbaine (AU) est crucial pour la survie alimentaire de l’Humanité. Des zones de maraîchage urbain, fermes verticales ou aquaponiques, jardins potagers collectifs (éventuellement sur les toits) ou privés avec poulaillers, arbres fruitiers, etc., se développent à l'échelle globale. Les villes sont caractérisées par des densités de population élevées, de fortes compétitions pour l’usage de l’espace, et des pollutions fréquemment observées. Ces caractéristiques ont des conséquences sur les formes d’AU qui peuvent durablement se développer: des projets participatifs multi-acteurs, efficients, multifonctions, à faibles intrants et porteurs d’améliorations écologiques et de valeurs humanistes telles l’autonomie et la solidarité. Cet ouvrage s’attache à éclairer les verrous à lever et les opportunités à saisir afin que l’agriculture urbaine se développe durablement grâce à notre intelligence collective et puisse pleinement jouer son rôle de levier pour promouvoir la transition écologique

Influence of fine process particles enriched with metals and metalloids on Lactuca sativa L. leaf fatty acid composition following air and/or soil-plant field exposure

We investigate the effect of both foliar and root uptake of a mixture of metal(loid)s on the fatty acid composition of plant leaves. Our objectives are to determine whether both contamination pathways have a similar effect and whether they interact. Lactuca sativa L. were exposed to fine process particles enriched with metal(loid)s in an industrial area. Data from a first experiment were used to conduct an exploratory statistical analysis which findings were successfully cross-validated by using the data from a second one. Both foliar and root pathways impact plant leaf fatty acid composition and do not interact. Z index (dimensionless quantity), weighted product of fatty acid concentration ratios was built up from the statistical analyses. It provides new insights on the mechanisms involved in metal uptake and phytotoxicity. Plant leaf fatty acid composition is a robust and fruitful approach to detect and understand the effects of metal(loid) contamination on plants

Scale Estimation with Dual Quadrics for Monocular Object SLAM

The scale ambiguity problem is inherently unsolvable to monocular SLAM without the metric baseline between moving cameras. In this paper, we present a novel scale estimation approach based on an object-level SLAM system. To obtain the absolute scale of the reconstructed map, we derive a nonlinear optimization method to make the scaled dimensions of objects conforming to the distribution of their sizes in the physical world, without relying on any prior information of gravity direction. We adopt the dual quadric to represent objects for its ability to fit objects compactly and accurately. In the proposed monocular object-level SLAM system, dual quadrics are fastly initialized based on constraints of 2-D detections and fitted oriented bounding box and are further optimized to provide reliable dimensions for scale estimation.Comment: 8 pages, 6 figures, accepted by IROS202

Green manure plants for remediation of soils polluted by metals and metalloids: Ecotoxicity and human bioavailability assessment

Borage, white mustard and phacelia, green manure plants currently used in agriculture to improve soil properties were cultivated for 10 wk on various polluted soils with metal(loid) concentrations representative of urban brownfields or polluted kitchen gardens. Metal(loid) bioavailability and ecotoxicity were measured in relation to soil characteristics before and after treatment. All the plants efficiently grow on the various polluted soils. But borage and mustard only are able to modify the soil characteristics and metal(loid) impact: soil respiration increased while ecotoxicity, bioaccessible lead and total metal(loid) quantities in soils can be decreased respectively by phytostabilization and phytoextraction mechanisms. These two plants could therefore be used for urban polluted soil refunctionalization. However, plant efficiency to improve soil quality strongly depends on soil characteristics

TiEV: The Tongji Intelligent Electric Vehicle in the Intelligent Vehicle Future Challenge of China

TiEV is an autonomous driving platform implemented by Tongji University of China. The vehicle is drive-by-wire and is fully powered by electricity. We devised the software system of TiEV from scratch, which is capable of driving the vehicle autonomously in urban paths as well as on fast express roads. We describe our whole system, especially novel modules of probabilistic perception fusion, incremental mapping, the 1st and the 2nd planning and the overall safety concern. TiEV finished 2016 and 2017 Intelligent Vehicle Future Challenge of China held at Changshu. We show our experiences on the development of autonomous vehicles and future trends

Overexpression of the aspartic protease ASPG1 gene confers drought avoidance in Arabidopsis

Drought is one of the most severe environmental stresses affecting plant growth and limiting crop production. Although many genes involved in adaptation to drought stress have been disclosed, the relevant molecular mechanisms are far from understood. This study describes an Arabidopsis gene, ASPG1 (ASPARTIC PROTEASE IN GUARD CELL 1), that may function in drought avoidance through abscisic acid (ABA) signalling in guard cells. Overexpression of the ASPG1 gene enhanced ABA sensitivity in guard cells and reduced water loss in ectopically overexpressing ASPG1 (ASPG1-OE) transgenic plants. In ASPG1-OE plants, some downstream targets in ABA and/or drought-signalling pathways were altered at various levels, suggesting the involvement of ASPG1 in ABA-dependent drought avoidance in Arabidopsis. By analysing the activities of several antioxidases including superoxide dismutase and catalase in ASPG1-OE plants, the existence was demonstrated of an effective detoxification system for drought avoidance in these plants. Analysis of ProASPG1-GUS lines showed a predominant guard cell expression pattern in various aerial tissues. Moreover, the protease activity of ASPG1 was characterized in vitro, and two aspartic acid sites, D180 and D379, were found to be key residues for ASPG1 aspartic protease activity in response to ABA. In summary, these findings suggest that functional ASPG1 may be involved in ABA-dependent responsiveness and that overexpression of the ASPG1 gene can confer drought avoidance in Arabidopsis

Natural ventilation potential for residential buildings in a densely built-up and highly polluted environment. A case study

The application of Natural Ventilation (NV) as a measure to improve comfort conditions in transition and summer periods has been a topic of research on the spotlight for years. However, there is a lack of knowledge about how the combined effect of a dense urban layout with high pollutant concentrations may affect its potential. This paper addresses this gap by running detailed thermal simulations for a typical apartment flat located in the Yuzhong district of Chongqing city (China) using a holistic approach that makes use of: i) wind pressure coefficients on building facades from urban-scale CFD simulations, ii) hourly measured values of PM2.5 concentrations and weather variables and iii) indoor environment measurements for validation purposes. Scenario analysis revealed the average amount of air change rates achievable in a year varies from 8 to 15 ACH according to the windows orientation. These figures drop down to around 2 ACH when taking into account reduced windows opening time when outdoor PM2.5 concentrations are too high. The resulting natural ventilation potential of the case study decreases from 4234 h when outdoor pollution is neglected to 2707 and 529 h when considering the exposure thresholds set by the Chinese government and the WHO respectively