Theoretical Study on Relaxed Surrounding Rock Pressure on Shallow Bias Neighborhood Tunnels under Seismic Load

To study the distribution of relaxed surrounding rock pressure on the shallow bias neighborhood tunnels under the combined action of horizontal and vertical earthquake force, finite element software was used for failure mode analysis. Moreover, with the pseudo-static method, the calculation formula for the relaxed pressure on the shallow bias neighborhood tunnels was derived and used to analyze the variation of the rupture angle of these tunnels under the action of the seismic force. The study shows that: shallow bias neighborhood tunnels basically follow a “W” failure pattern under the combined action of horizontal and vertical seismic force, and the failure scope of the surrounding rock is controlled by four rupture angles. Rupture angles β2 and β3 between the deep and shallow tunnels of the shallow bias neighborhood tunnels are not affected by the surface slope. For tunnels with the same grade of the surrounding rock, the greater the seismic intensity, the smaller the value of β2, and the greater the value of β3. While at the same seismic intensity, the higher the grade of the surrounding rock, the smaller the β2 and β3. Ruptures angles β1 and β4 are influenced by the surface slope, seismic intensity and surrounding rock grades. A steeper surface slope leads to a smaller β1 and a greater β4; β1 increase and β4 decrease with increasing seismic intensity; while, β1 and β4 both show a decreasing trend with an increasing rock grade

Basalt-polypropylene fiber reinforced concrete for durable and sustainable pipe production. Part 1: experimental program

This is the peer reviewed version of the following article: [ Deng, Z, Liu, X, Chen, P, et al. Basalt-polypropylene fiber reinforced concrete for durable and sustainable pipe production. Part 1: Experimental program. Structural Concrete. 2022; 23: 311– 327. https://doi.org/10.1002/suco.202000759], which has been published in final form at https://onlinelibrary.wiley.com/doi/abs/10.1002/suco.202000759. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.An experimental program consisting in producing and testing reinforced concrete pipes (RCPs) under the three-edge bearing tests considering different types of reinforcement was carried out. Four types of RCPs were produced, these reinforced with: (1) polypropylene macrofibers; (2) basalt microfibers; (3) combination of both (hybrid reinforcement); and (4) plain concrete. The analysis of the crack patterns and both service and ultimate mechanical responses allowed concluding that the use of fibers do not lead to an effective increase of the first cracking load; however, both types of fibers allowed a better crack width control respect to the standard RCP. In this regard, basalt microfiber reinforced concrete led to a better response caused by concentrated loads (jacketing) whilst polypropylene macrofibers increased the concrete pipe performance in terms of bearing capacity and flexural crack control. The hybrid fiber reinforced concrete was found to be the most suitable alternative for increasing the load bearing capacity and the crack width control for service loads. These incipient experimental results permit to conclude that this type of hybrid basalt-polypropylene fiber reinforced concretes are an interesting alternative to traditional steel-cage RCPs.This work is supported by the National Key Research and Development Program of China (2018YFC1504802), Natural Science Foundation Project of Chongqing, Chongqing Science and Technology Commission (cstc2018jscxmszdX0071), Postgraduate Research Innovation Project of Chongqing (CYS19005, CYS18026). In addition, Prof. Albert de la Fuente also wants to express his gratitude to the Spanish Ministry of Science and Innovation for the financial support received under the scope of the project CREEF (PID2019-108978RB-C32).Peer ReviewedPostprint (author's final draft

Global assessment of spatiotemporal changes of frequency of terrestrial wind speed

Wind energy, an important component of clean energy, is highly dictated by the disposable wind speed within the working regime of wind turbines (typically between 3 and 25 m s−1 at the hub height). Following a continuous reduction ('stilling') of global annual mean surface wind speed (SWS) since the 1960s, recently, researchers have reported a 'reversal' since 2011. However, little attention has been paid to the evolution of the effective wind speed for wind turbines. Since wind speed at hub height increases with SWS through power law, we focus on the wind speed frequency variations at various ranges of SWS through hourly in-situ observations and quantify their contributions to the average SWS changes over 1981–2021. We found that during the stilling period (here 1981–2010), the strong SWS (⩾ 5.0 m s−1, the 80th of global SWS) with decreasing frequency contributed 220.37% to the continuous weakening of mean SWS. During the reversal period of SWS (here 2011–2021), slight wind (0 m s−1 < SWS < 2.9 m s−1) contributed 64.07% to a strengthening of SWS. The strengthened strong wind (⩾ 5.0 m s−1) contributed 73.38% to the trend change of SWS from decrease to increase in 2010. Based on the synthetic capacity factor series calculated by considering commercial wind turbines (General Electric GE 2.5-120 model with rated power 2.5 MW) at the locations of the meteorological stations, the frequency changes resulted in a reduction of wind power energy (−10.02 TWh yr−1, p < 0.001) from 1981 to 2010 and relatively weak recovery (2.67 TWh yr−1, p < 0.05) during 2011–2021.This study was supported by the National Natural Science Foundation of China (Grant No. 42071022), Guangdong Basic and Applied Basic Research Fund (2022A1515240070) and the start-up fund provided by Southern University of Science and Technology (no. 29/Y01296122). C A-M was supported by the IBER-STILLING (RTI2018-095749-A-I00, MCIU/AEI/FEDER,UE); VENTS (GVA-AICO/2021/023); the CSIC Interdisciplinary Thematic Platform (PTI) Clima (PTI-CLIMA); and the 2021 Leonardo Grant for Researchers and Cultural Creators, BBVA Foundation. RJHD was supported by the Met Office Hadley Centre Climate Programme funded by BEIS and by the UK-China Research & Innovation Partnership Fund through the Met Office Climate Science for Service Partnership (CSSP) China as part of the Newton Fund. SJ was supported by the Ramon y Cajal program and the OPEN project (RYC2020-029993-I and TED2021-131074B-I00, MCIU/AEI/FEDER,UE)

Profile and clinical implication of circular RNAs in human papillary thyroid carcinoma

Background Differently expressed circular RNAs (circRNAs) have been reported to play a considerable role in tumor behavior; however, the expression profile and biological function of circRNAs in papillary thyroid carcinoma (PTC) remains unknown. Thus, the study was aimed to characterize the circRNA expression profile to comprehensively understand the biological behavior of PTC. Methods We investigated the expression profile of circRNAs using circRNA microarray in three pairs of PTC and adjacent normal tissues. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) was used to validate eight candidate circRNAs in 40 paired PTC tumors and adjacent normal samples. Next, we employed a bioinformatics tool to identify putative miRNA and circRNA-associated downstream genes, followed by constructing a network map of circRNA-miRNA-mRNA interactions and exploring the potential role of the candidate circRNAs. Results In total, 206 up- and 177 downregulated circRNAs were identified in PTC tissues (fold change >1.5; P < 0.05). The expression levels of eight candidate circRNAs confirmed by qRT-PCR were significantly different between the PTC and normal samples. The downstream genes of candidate circRNAs participated in various biological processes and signaling pathways. The most up and downregulated circRNAs were hsa_circRNA_007148 and hsa_circRNA_047771. The lower expression level of hsa_circRNA_047771 was associated BRAFV600 mutation, lymph node metastasis (LNM), as well as with advanced TNM stage (all P < 0.05). The higher expression level of hsa_circRNA_007148 was significantly correlated with LNM (P < 0.05). The areas under receiver operating curve were 0.876 (95% CI [0.78–0.94]) for hsa_circRNA_047771 and 0.846 (95% CI [0.75–0.96]) for hsa_circRNA_007148. Discussion The study suggests that dysregulated circRNAs play a critical role in PTC pathogenesis. PTC-related hsa_circRNA_047771 and hsa_circRNA_007148 may serve as potential diagnostic biomarkers and prognostic predictors for PTC patients

Pseudomonas aeruginosa Quorum-Sensing and Type VI Secretion System Can Direct Interspecific Coexistence During Evolution

It is reported that a wide range of bacterial infections are polymicrobial, and the members in a local microcommunity can influence the growth of neighbors through physical and chemical interactions. Pseudomonas aeruginosa is an important opportunistic pathogen that normally causes a variety of acute and chronic infections, and clinical evidences suggest that P. aeruginosa can be frequently coisolated with other pathogens from the patients with chronic infections. However, the interspecific interaction and the coexisting mechanism of P. aeruginosa with coinfecting bacterial species during evolution still remain largely unclear. In this study, the relationships of P. aeruginosa with other Gram-positive (Staphylococcus aureus) and Gram-negative (Klebsiella pneumoniae) are investigated by using a series of on-plate proximity assay, in vitro coevolution assay, and RNA-sequencing. We find that although the development of a quorum-sensing system contributes P. aeruginosa a significant growth advantage to compete with S. aureus and K. pneumoniae, the quorum-sensing regulation of P. aeruginosa will be decreased during evolution and thus provides a basis for the formation of interspecific coexistence. The results of comparative transcriptomic analyses suggest that the persistent survival of S. aureus in the microcommunity has no significant effect on the intracellular transcriptional pattern of P. aeruginosa, while a more detailed competition happens between P. aeruginosa and K. pneumoniae. Specifically, the population of P. aeruginosa with decreased quorum-sensing regulation can still restrict the proportion increase of K. pneumoniae by enhancing the type VI secretion system-elicited cell aggressivity during further coevolution. These findings provide a general explanation for the formation of a dynamic stable microcommunity consisting of more than two bacterial species, and may contribute to the development of population biology and clinical therapy

Molecular Modeling Study of Chiral Separation and Recognition Mechanism of β-Adrenergic Antagonists by Capillary Electrophoresis

Chiral separations of five β-adrenergic antagonists (propranolol, esmolol, atenolol, metoprolol, and bisoprolol) were studied by capillary electrophoresis using six cyclodextrins (CDs) as the chiral selectors. Carboxymethylated-β-cyclodextrin (CM-β-CD) exhibited a higher enantioselectivity power compared to the other tested CDs. The influences of the concentration of CM-β-CD, buffer pH, buffer concentration, temperature, and applied voltage were investigated. The good chiral separation of five β-adrenergic antagonists was achieved using 50 mM Tris buffer at pH 4.0 containing 8 mM CM-β-CD with an applied voltage of 24 kV at 20 °C. In order to understand possible chiral recognition mechanisms of these racemates with CM-β-CD, host-guest binding procedures of CM-β-CD and these racemates were studied using the molecular docking software Autodock. The binding free energy was calculated using the Autodock semi-empirical binding free energy function. The results showed that the phenyl or naphthyl ring inserted in the hydrophobic cavity of CM-β-CD and the side chain was found to point out of the cyclodextrin rim. Hydrogen bonding between CM-β-CD and these racemates played an important role in the process of enantionseparation and a model of the hydrogen bonding interaction positions was constructed. The difference in hydrogen bonding formed with the –OH next to the chiral center of the analytes may help to increase chiral discrimination and gave rise to a bigger separation factor. In addition, the longer side chain in the hydrophobic phenyl ring of the enantiomer was not beneficial for enantioseparation and the chiral selectivity factor was found to correspond to the difference in binding free energy

Functional genomic screening identifies dual leucine zipper kinase as a key mediator of retinal ganglion cell death

Glaucoma, a major cause of blindness worldwide, is a neurodegenerative optic neuropathy in which vision loss is caused by loss of retinal ganglion cells (RGCs). To better define the pathways mediating RGC death and identify targets for the development of neuroprotective drugs, we developed a high-throughput RNA interference screen with primary RGCs and used it to screen the full mouse kinome. The screen identified dual leucine zipper kinase (DLK) as a key neuroprotective target in RGCs. In cultured RGCs, DLK signaling is both necessary and sufficient for cell death. DLK undergoes robust posttranscriptional up-regulation in response to axonal injury in vitro and in vivo. Using a conditional knockout approach, we confirmed that DLK is required for RGC JNK activation and cell death in a rodent model of optic neuropathy. In addition, tozasertib, a small molecule protein kinase inhibitor with activity against DLK, protects RGCs from cell death in rodent glaucoma and traumatic optic neuropathy models. Together, our results establish a previously undescribed drug/drug target combination in glaucoma, identify an early marker of RGC injury, and provide a starting point for the development of more specific neuroprotective DLK inhibitors for the treatment of glaucoma, nonglaucomatous forms of optic neuropathy, and perhaps other CNS neurodegenerations