A Comparison of MERRA and NARR Reanalysis Datasets with the DOE ARM SGP Continuous Forcing data

In this study, the atmospheric state, precipitation, cloud fraction, and radiative fluxes from Modern Era Retrospective-analysis for Research and Applications (MERRA) and North American Regional Reanalysis (NARR) are collected and compared with the Atmospheric Radiation Measurement (ARM) Southern Great Plains (SGP) continuous forcing during the period 1999-2001. For the atmospheric state, the three datasets have excellent agreement for the horizontal wind components and air temperature. NARR and ARM have generally good agreement for humidity, except for several biases in the PBL and in the upper troposphere. MERRA, on the other hand, suffers from a year-round negative bias in humidity except for the month of June. For the vertical pressure velocity, significant differences exist with the largest biases occurring during the spring upwelling and summer downwelling periods. Although NARR and MERRA share many resemblances to each other, ARM outperforms these reanalyses in terms of correlation with cloud fraction. Because the ARM forcing is constrained by observed precipitation that gives the adequate mass, heat, and moisture budgets, much of the precipitation (specifically during the late spring/early summer) is caused by smaller-scale forcing that is not captured by the reanalyses. Both NARR and MERRA capture the seasonal variation of CF observed by ARM radar-lidar and GOES with high correlations (0.92-0.78), but having negative biases of 14% and 3%, respectively. Compared to the ARM observations, MERRA shows a better agreement for both SW and LW fluxes except for LW-down (due to a negative bias in water vapor), NARR has significant positive bias for SW-down and negative bias for LW-down under clear- and all-sky conditions . The NARR biases result from a combination of too few clouds and a lack of sufficient extinction by aerosols and water vapor in the atmospheric column. The results presented here represent only one location for a limited time period, and more comparisons at different locations and longer time period are needed

Life Cycle of Midlatitude Deep Convective Systems in a Lagrangian Framework

Deep Convective Systems (DCSs) consist of intense convective cores (CC), large stratiform rain (SR) regions, and extensive non-precipitating anvil clouds (AC). This study focuses on the evolution of these three components and the factors that affect convective AC production. An automated satellite tracking method is used in conjunction with a recently developed multi-sensor hybrid classification to analyze the evolution of DCS structure in a Lagrangian framework over the central United States. Composite analysis from 4221 tracked DCSs during two warm seasons (May-August, 2010-2011) shows that maximum system size correlates with lifetime, and longer-lived DCSs have more extensive SR and AC. Maximum SR and AC area lag behind peak convective intensity and the lag increases linearly from approximately 1-hour for short-lived systems to more than 3-hours for long-lived ones. The increased lag, which depends on the convective environment, suggests that changes in the overall diabatic heating structure associated with the transition from CC to SR and AC could prolong the system lifetime by sustaining stratiform cloud development. Longer-lasting systems are associated with up to 60% higher mid-tropospheric relative humidity and up to 40% stronger middle to upper tropospheric wind shear. Regression analysis shows that the areal coverage of thick AC is strongly correlated with the size of CC, updraft strength, and SR area. Ambient upper tropospheric wind speed and wind shear also play an important role for convective AC production where for systems with large AC (radius greater than 120-km) they are 24% and 20% higher, respectively, than those with small AC (radius=20 km)

Proteomic analysis of differential anther development from sterile/fertile lines in Capsicum annuum L.

Background Pepper (Capsicum annuum L.) is a major cash crop throughout the world. Male sterility is an important characteristic in crop species that leads to a failure to produce functional pollen, and it has crucial roles in agricultural breeding and the utilization of heterosis. Objectives In this study, we identified many crucial factors and important components in metabolic pathways in anther and pollen development, and elucidated the molecular mechanism related to pollen abortion in pepper. Methods Pepper pollen was observed at different stages to detect the characteristics associated with male sterility and fertility. The phytohormone and oxidoreductase activities were detected in spectrophotometric and redox reaction assays, respectively. Proteins were extracted from male sterile and fertile pepper lines, and identified by TMT/iTRAQ (tandem mass tags/isobaric tags for relative and absolute quantitation) and LC-MS/MS (liquid chromatograph-mass spectrometer) analysis. Differentially abundant proteins (DAPs) were analyzed based on Gene Ontology annotations and the Kyoto Encyclopedia of Genes and Genomes database according to |fold change)| > 1.3 and P value < 0.05. DAPs were quantified in the meiosis, tetrad, and binucleate stages by parallel reaction monitoring (PRM). Results In this study, we screened and identified one male sterile pepper line with abnormal cytological characteristics in terms of pollen development. The peroxidase and catalase enzyme activities were significantly reduced and increased, respectively, in the male sterile line compared with the male fertile line. Phytohormone analysis demonstrated that the gibberellin, jasmonic acid, and auxin contents changed by different extents in the male sterile pepper line. Proteome analysis screened 1,645 DAPs in six clusters, which were mainly associated with the chloroplast and cytoplasm based on their similar expression levels. According to proteome analysis, 45 DAPs were quantitatively identified in the meiosis, tetrad, and binucleate stages by PRM, which were related to monoterpenoid biosynthesis, and starch and sucrose metabolism pathways. Conclusions We screened 1,645 DAPs by proteomic analysis and 45 DAPs were related to anther and pollen development in a male sterile pepper line. In addition, the activities of peroxidase and catalase as well as the abundances of phytohormones such as gibberellin, jasmonic acid, and auxin were related to male sterility. The results obtained in this study provide insights into the molecular mechanism responsible for male sterility and fertility in pepper