Thermoelectric Textile Materials

Textile, as an intimate partner of human body, shows promising application in wearable thermoelectrics for body heat conversion. Compared with other widely studied flexible film thermoelectric materials, textiles having better air-permeability, wearability, and flexibility are more suitable for wearable electronics. In the past few years, many researches have focused on the design and fabrication of textile-based thermoelectric materials and generators. By integrating with high performance inorganic semiconductors and conductive polymers, fabrics or fibers will be given thermoelectric properties. Technologies of coating, printing, and even thermal drawing can be adopted in the fabrication of textile thermoelectric materials. With great design flexibility, various flexible textile generator structures can be obtained by using yarns or fabrics as thermoelectric legs, which will bring new inspirations for the future development of flexible thermoelectrics

Special Behaviors in One Pedestrian Flow Experiment

We organize one pedestrian flow experiment with 278 participants, and the maximum density reaches 9 ped/(m^2). The experiment is filmed by one UAV, and in the experimental video, we find some interesting behaviors. Five types of these behaviors are classified and introduced: 1) oppression near the boundaries; 2) impact on the boundaries; 3) special moves; 4) absentmindedness; 5) other events. The numbers of Type 1 and 2 behaviors can be counted, while the frequencies of Type 3 and 4 behaviors can be roughly estimated. At one critical density, the results of Type 1, 2, 3, 4 behaviors qualitatively change. This value is about 7~8 ped/(m^2), which indicates the possible existence of critical phenomena in pedestrian flow

The Impact of General Self-Efficacy on Psychological Resilience During the COVID-19 Pandemic: The Mediating Role of Posttraumatic Growth and the Moderating Role of Deliberate Rumination

Purpose: This study used a moderated mediation model to explore the relationship between general self-efficacy (GSE) and psychological resilience (PR) and the associated mechanisms, the mediating role of posttraumatic growth (PTG), and the moderating role of deliberate rumination (DR) during the coronavirus disease 2019 (COVID-19) pandemic. Knowledge of the relationship between these four variables examined further understanding of the PR improvement mechanism of college students and even the general public.Methods: The college students who participated in this study came from an independent college in Guangdong Province, China. A total of 918 college students completed the survey, and the final data sample size was 881. SPSS 23.0 and PROCESS (version 3.3) were used to conduct Pearson's correlation analysis and hierarchical regression linear analysis on the data.Results: (1) The correlation analysis showed that GSE and PR were positively correlated and that PTG was positively correlated with GSE and PR. DR was positively correlated with GSE, PTG, and PR. (2) The results of mediation analysis showed that GSE had a direct predictive effect on DR, and PTG partially mediated the relationship between the two. (3) The results of moderating effect analysis showed that DR hindered the effect of GSE on PTG but enhanced the positive impact of PTG on PR.Conclusions: General self-efficacy can improve PR under the mediating influence of PTG. DR played a positive moderating role in the relationship between GSE and PTG, and played a negative moderating role in the relationship between PTG and PR. These results advance the understanding of the mechanism between GSE and PR

Improvement of resistance to rice blast and bacterial leaf streak by CRISPR/Cas9-mediated mutagenesis of Pi21 and OsSULTR3;6 in rice (Oryza sativa L.)

Rice (Oryza sativa L.) is a staple food in many countries around the world, particularly in China. The production of rice is seriously affected by the bacterial leaf streak and rice blast, which can reduce rice yield or even cause it to fail to be harvested. In this study, susceptible material 58B was edited by CRISPR/Cas9, targeting a target of the Pi21 gene and a target of the effector-binding element (EBE) of the OsSULTR3;6 gene, and the mutants 58b were obtained by Agrobacterium-mediated method. The editing efficiency of the two targets in the T0 generation was higher than 90.09%, the homozygous mutants were successfully selected in the T0 generation, and the homozygous mutation rate of each target was higher than 26.67%. The expression of the edited pi21 and EBE of Ossultr3;6 was significantly reduced, and the expression of defense responsive genes was significantly upregulated after infected with rice blast. The lesion areas of rice blast and bacterial leaf streak were significantly reduced in 58b, and the resistance of both was effectively improved. Furthermore, the gene editing events did not affect the agronomic traits of rice. In this study, the resistance of 58b to rice blast and bacterial leaf streak was improved simultaneously. This study provides a reference for using Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 (CRISPR/Cas9) to accelerate the improvement of rice varieties and the development of new materials for rice breeding

Dynamics of human replication factors in the elongation phase of DNA replication

In eukaryotic cells, DNA replication is carried out by coordinated actions of many proteins, including DNA polymerase δ (pol δ), replication factor C (RFC), proliferating cell nuclear antigen (PCNA) and replication protein A. Here we describe dynamic properties of these proteins in the elongation step on a single-stranded M13 template, providing evidence that pol δ has a distributive nature over the 7 kb of the M13 template, repeating a frequent dissociation–association cycle at growing 3′-hydroxyl ends. Some PCNA could remain at the primer terminus during this cycle, while the remainder slides out of the primer terminus or is unloaded once pol δ has dissociated. RFC remains around the primer terminus through the elongation phase, and could probably hold PCNA from which pol δ has detached, or reload PCNA from solution to restart DNA synthesis. Furthermore, we suggest that a subunit of pol δ, POLD3, plays a crucial role in the efficient recycling of PCNA during dissociation–association cycles of pol δ. Based on these observations, we propose a model for dynamic processes in elongation complexes

Fan Fault Diagnosis Based on Wavelet Packet and Sample Entropy

To accurately diagnose the mechanical failure of the fan, two diagnostic methods based on the wavelet packet energy feature and sample entropy feature are proposed. Vibration signals acquisition of 13 kinds of running states are achieved on the 4-73 No.8D centrifugal fan test bench. The wavelet packet energy feature vector of each vibration signal is rapidly extracted through the wavelet packet denoising, decomposition and reconstruction. The vibration signal wavelet packet energy feature vector of the five measuring points in the same instantaneous running state are fused into the fan fault feature vector. Finally, the fault diagnosis of the fan is achieved by using improved SVM (Support Vector Machine) classifier, and the accuracy rate is 94.6%. A new fan fault feature vector is put forward, which is the integration of the vibration signal sample entropy of the five measuring points in the same instantaneous running state, and then the fault diagnosis of the fan is achieved by using improved BP (Back Propagation) neural network, and the accuracy rate is 99.23%. The diagnostic results show that these two methods are able to effectively diagnose the category, severity and site of the fan mechanical failures, and suitable for online diagnosis. DOI: http://dx.doi.org/10.11591/telkomnika.v11i6.272

Effect of EVA and DCP addition on injection moldability and tensile properties of recycled PE from disposable drip tapes

The effects of ethylene-vinyl acetate copolymer (EVA) and dicumyl peroxide (DCP) addition on injection moldability and tensile properties of recycled polyethylene (PE) from disposable drip tapes were studied. DCP showed an adverse impact on the injection moldability of recycled PE materials, while EVA had positive effects on it. Tensile properties of the reproduced PE increased with the increasing amount of EVA up to 10 wt.% and decreased beyond it. The DCP addition reduced the elastic properties of the reproduced PE, while it enhanced the tensile strength. Overall, the co-addition of 6 wt.% EVA + 0.4 wt.% DCP could improve the tensile strength of the reproduced PE materials

Effects of Cd(2+) and Pb(2+) on the substrate biofilms in the integrated vertical-flow constructed wetland

The effects of single Cd(2+) and Pb(2+), and combined Cd(2+) and Pb(2+) on dehydrogenase activity and polysaccharide content of the substrate biofilms in the integrated vertical-flow constructed wetland (IVCW) were studied. Dehydrogenase activities decreased linearly with the increasing concentrations of Cd(2+) and Pb(2+) at different times (6, 24, 72, and 120 h). The activities at both 6 and 24 h were significantly higher than that at 72 and 120 h in the case of single and combined treatments. The single Cd(2+) and Pb(2+) treatments significantly inhibited dehydrogenase activities at concentrations in excess of 20 mu mol/L Cd(2+) and 80 mu mol/L Pb(2+), respectively. The inhibitory effect of Cd(2+) was much greater than that of Pb(2+). At the same time, the combined treatment of Cd(2+) and Pb(2+) Significantly inhibited dehydrogenase activities at all five concentrations studied and the lowest combined concentration was 1.25 mu mol/L Cd(2+) and 5 mu mol/L Pb(2+). A synergistic effect of Cd(2+) and Pb(2+) was observed. On the other hand, polysaccharide contents varied unpredictably with the increasing concentrations of Cd(2+) and Pb(2+) and extended experimental time. There were no significant statistical differences within the range of concentration and time studied, whether singly or in combination. These results implied that the effects of heavy metals on biofilms should be a concern for the operation and maintenance of constructed wetlands

Multipotent male germline stem cells (mGSCs) from neonate porcine testis

ABSTRACT Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis, during which unlimited spermatozoa is produced daily derived from SSCs in the testis throughout life of the male. Germline stem (GS) cells can be isolated from spermatogonia, which shared the characteristics of SSCs and embryonic stem cells (ESCs), and can be passaged stably in vitro. The study of GS cells contributes to understanding spermatogenesis process. However, little is known about the GS cells in domestic animals. Here, we report the successful establishment of a serum- and feeder-free system for multipotent male GS cells (mGSCs) from postnatal porcine testis. These cells expressed pluripotent markers, such as Oct4, Nanog, C-myc, and germline-specific markers including Vasa, CD90, CD49f, Gfrα1, Plzf and Dazl. Then we assayed the developmental potential of these cells in vitro. The porcine multipotent male germline stem cells (pmGSCs) can form embryoid bodies (EBs) by suspension culture. Immunofluorescence analysis showed that the EBs differentiated into neuron-specific enolase (NSE, ectoderm), α-actin (mesoderm), and Pdx1 (endoderm) positive cells. These cells induced by 10-6 M retinoic acid (RA) could be differentiated into spermatid-like cells which were positive for Acrosin. The pmGSCs has been cultured over 14 passages. Thus, we have established a long-term culture system for pmGSCs. This culture system provides a platform for the study of porcine mGSCs