810 research outputs found

    A direct and inverse boundary layer method for subsonic flow over delta wings

    Get PDF
    A new inverse boundary layer method is developed and applied to incompressible flows with laminar separation and reattachment. Test cases for two dimensional flows are computed and the results are compared with those of other inverse methods. One advantage of the present method is that the calculation of the inviscid velocities may be determined at each marching step without having to iterate. The inverse method was incorporated with the direct method to calculate the incompressible, conical flow over a slender delta wing at incidence. The location of the secondary separation line on the leeward surface of the wing is determined and compared with experiment for a unit aspect ratio wing at 20.5 deg incidence. The viscous flow in the separated region was calculated using prescribed skin friction coefficients

    Integrability of a conducting elastic rod in a magnetic field

    Full text link
    We consider the equilibrium equations for a conducting elastic rod placed in a uniform magnetic field, motivated by the problem of electrodynamic space tethers. When expressed in body coordinates the equations are found to sit in a hierarchy of non-canonical Hamiltonian systems involving an increasing number of vector fields. These systems, which include the classical Euler and Kirchhoff rods, are shown to be completely integrable in the case of a transversely isotropic rod; they are in fact generated by a Lax pair. For the magnetic rod this gives a physical interpretation to a previously proposed abstract nine-dimensional integrable system. We use the conserved quantities to reduce the equations to a four-dimensional canonical Hamiltonian system, allowing the geometry of the phase space to be investigated through Poincar\'e sections. In the special case where the force in the rod is aligned with the magnetic field the system turns out to be superintegrable, meaning that the phase space breaks down completely into periodic orbits, corresponding to straight twisted rods.Comment: 19 pages, 1 figur

    Democratically engaged assessment: Reimagining the purposes and practices of assessment in community engagement

    Get PDF
    This document is a project of reclamation and transformation, one that is both ongoing and rooted in years of dialogue within Imagining America and the work of its Assessing Practices of Public Scholarship research group (APPS). It emerges from our own experiences with assessment related to community engagement and from those of many other colleagues on campuses and in diverse communities. It is intended to bring together those who wish to reimagine assessment in light of its civic potential — to develop what we refer to as Democratically Engaged Assessment (DEA).Imagining Americ

    Probing complex RNA structures by mechanical force

    Full text link
    RNA secondary structures of increasing complexity are probed combining single molecule stretching experiments and stochastic unfolding/refolding simulations. We find that force-induced unfolding pathways cannot usually be interpretated by solely invoking successive openings of native helices. Indeed, typical force-extension responses of complex RNA molecules are largely shaped by stretching-induced, long-lived intermediates including non-native helices. This is first shown for a set of generic structural motifs found in larger RNA structures, and then for Escherichia coli's 1540-base long 16S ribosomal RNA, which exhibits a surprisingly well-structured and reproducible unfolding pathway under mechanical stretching. Using out-of-equilibrium stochastic simulations, we demonstrate that these experimental results reflect the slow relaxation of RNA structural rearrangements. Hence, micromanipulations of single RNA molecules probe both their native structures and long-lived intermediates, so-called "kinetic traps", thereby capturing -at the single molecular level- the hallmark of RNA folding/unfolding dynamics.Comment: 9 pages, 9 figure

    Plasma Dynamics

    Get PDF
    Contains reports on three research projects.Wright Air Development Division (Contract AF33(616)-3984)United States Atomic Energy Commission (Contract AT(30-1)-1842)National Science Foundation (Grant G-9330)United States Air Force, Air Force Cambridge Research Center (Contract AF19(604)-4551)United States Air Force, Air Force Cambridge Research Center, Air Research and Development Command (Contract AF19(604)-5992

    Genetic and transcriptomic analysis of transcription factor genes in the model halophilic Archaeon: coordinate action of TbpD and TfbA

    Get PDF
    <p>Abstract</p> <p>Background</p> <p>Archaea are prokaryotic organisms with simplified versions of eukaryotic transcription systems. Genes coding for the general transcription factors TBP and TFB are present in multiple copies in several Archaea, including <it>Halobacterium </it>sp. NRC-1. Multiple TBP and TFBs have been proposed to participate in transcription of genes via recognition and recruitment of RNA polymerase to different classes of promoters.</p> <p>Results</p> <p>We attempted to knock out all six TBP and seven TFB genes in <it>Halobacterium </it>sp. NRC-1 using the <it>ura</it>3-based gene deletion system. Knockouts were obtained for six out of thirteen genes, <it>tbp</it>CDF and <it>tfb</it>ACG, indicating that they are not essential for cell viability under standard conditions. Screening of a population of 1,000 candidate mutants showed that genes which did not yield mutants contained less that 0.1% knockouts, strongly suggesting that they are essential. The transcriptomes of two mutants, Δ<it>tbp</it>D and Δ<it>tfb</it>A, were compared to the parental strain and showed coordinate down regulation of many genes. Over 500 out of 2,677 total genes were regulated in the Δ<it>tbp</it>D and Δ<it>tfb</it>A mutants with 363 regulated in both, indicating that over 10% of genes in both strains require the action of both TbpD and TfbA for normal transcription. Culturing studies on the Δ<it>tbp</it>D and Δ<it>tfb</it>A mutant strains showed them to grow more slowly than the wild-type at an elevated temperature, 49°C, and they showed reduced viability at 56°C, suggesting TbpD and TfbA are involved in the heat shock response. Alignment of TBP and TFB protein sequences suggested the expansion of the TBP gene family, especially in <it>Halobacterium </it>sp. NRC-1, and TFB gene family in representatives of five different genera of haloarchaea in which genome sequences are available.</p> <p>Conclusion</p> <p>Six of thirteen TBP and TFB genes of <it>Halobacterium </it>sp. NRC-1 are non-essential under standard growth conditions. TbpD and TfbA coordinate the expression of over 10% of the genes in the NRC-1 genome. The Δ<it>tbp</it>D and Δ<it>tfb</it>A mutant strains are temperature sensitive, possibly as a result of down regulation of heat shock genes. Sequence alignments suggest the existence of several families of TBP and TFB transcription factors in <it>Halobacterium </it>which may function in transcription of different classes of genes.</p

    Plasma Dynamics

    Get PDF
    Contains research objectives and reports on three research projects.National Science Foundation under Grant G-9930Air Force Cambridge Research Center under Contract AF-19(604)-5992WADC Contract AF33(616)-3984, with the Electronic Systems LaboratoryContract AF19(604)-4551 with Air Force Cambridge Research CenterAtomic Energy Commission under Contract AT(30-1)184

    Plasma Magnetohydrodynamics and Energy Conversion

    Get PDF
    Contains reports on eight research projects.National Science Foundation (Grant G-24073)United States Air Force, Aeronautical Systems Division, Aeronautical Accessories Laboratory, Wright-Patterson Air Force Base (Contract AF33(616)-7624)United States Air Force, Office of Scientific Research of the Office of Aerospace Research (Research Grant No. 62-308
    corecore