Democratically engaged assessment: Reimagining the purposes and practices of assessment in community engagement

This document is a project of reclamation and transformation, one that is both ongoing and rooted in years of dialogue within Imagining America and the work of its Assessing Practices of Public Scholarship research group (APPS). It emerges from our own experiences with assessment related to community engagement and from those of many other colleagues on campuses and in diverse communities. It is intended to bring together those who wish to reimagine assessment in light of its civic potential — to develop what we refer to as Democratically Engaged Assessment (DEA).Imagining Americ

Geostatistical Characterization of the Spatial Distribution of Adult Corn Rootworm (Coleoptera: Chrysomelidae) Emergence

Geostatistical methods were used to characterize spatial variability in western ( Diabrotica virgifera virgifera LeConte) and northern ( Diabrotica barberi Smith & Lawrence) corn rootworm adult emergence patterns. Semivariograms were calculated for adult emergence density of corn rootworm populations in fields of continuous corn and rotated (corn/soybean) corn. Adult emergence densities were generally greater for northern corn rootworms than for western corn rootworms. The spatial structures of the adult rootworm emergence were aggregated as described by spherical spatial models for western corn rootworm and exponential models for northern corn rootworm. Range of spatial dependence varied from 180 to 550 m for western corn rootworm and 172 to 281 m for northern corn rootworm. Semivariograrn models were used to produce contour density maps of adult populations in the fields, based on grid sampling of actual emerging adult populations

Field Scale Variability of Nitrogen and δ15N in Soil and Plants

Understanding the factors that influence soil and plant nitrogen (N) spatial variability may improve our ability to develop management systems that maximize productivity and minimize environmental hazards. The objective of this study was to determine the field (65 ha) scale spatial variability of N and δ15N in soil and corn (Zea mays). Soil, grain, and stover samples were collected from grids that ranged in size from 30 by 30 m to 60 by 60 m. Plant samples, collected following physiological maturity in 1995, were analyzed for total N and δ15N. Soil samples, collected prior to planting in the spring of 1995 and 1996, were analyzed for inorganic‐N, total N, and δ15N. All parameters showed strong spatial relationships. In an undrained portion of the field containing somewhat poorly and poorly drained soils there was a net loss of 95 kg N ha‐1, while in an adjacent area that was tile drained there was a net gain of 98 kg N ha‐1. Denitrification and N mineralization most likely were responsible for losses and gains, respectively. Differences between the N balances of these areas (193 kg N ha‐1) provide a relative measure of the impact of tile drainage on plant N availability and greenhouse gas production in a wet year

Integrability of a conducting elastic rod in a magnetic field

We consider the equilibrium equations for a conducting elastic rod placed in a uniform magnetic field, motivated by the problem of electrodynamic space tethers. When expressed in body coordinates the equations are found to sit in a hierarchy of non-canonical Hamiltonian systems involving an increasing number of vector fields. These systems, which include the classical Euler and Kirchhoff rods, are shown to be completely integrable in the case of a transversely isotropic rod; they are in fact generated by a Lax pair. For the magnetic rod this gives a physical interpretation to a previously proposed abstract nine-dimensional integrable system. We use the conserved quantities to reduce the equations to a four-dimensional canonical Hamiltonian system, allowing the geometry of the phase space to be investigated through Poincar\'e sections. In the special case where the force in the rod is aligned with the magnetic field the system turns out to be superintegrable, meaning that the phase space breaks down completely into periodic orbits, corresponding to straight twisted rods.Comment: 19 pages, 1 figur

Diurnal heat balance for the northern Monterey Bay inner shelf

In the summer of 2007, physical measurements including velocity from acoustic Doppler current profilers, surface gravity wave heights measured acoustically, and temperature from thermistor chain arrays were collected along- and across- the mid to inner shelf (water depths from 10–60 m) in northern Monterey Bay. The oceanic response to a strong (8–15 m s⁻¹ daily maximum) along-shelf sea breeze is examined by evaluating the diurnal heat budget over a cross-shelf section of the inner shelf. The diurnal heat budget closes to within the 95% confidence level with daily warming and cooling periods explained by two separate, but related processes. During evening/early morning warming period, 77% of the observed temperature increase is due to along-shelf advection of a temperature gradient within the upwelling shadow zone, a process which is arrested during the period of wind-forcing. In contrast, 75% of the afternoon cooling period is explained by the cross-shelf heat flux driven by diurnal along-shelf winds. In this study, diurnal tides are found to contribute less than 10% of the observed temperature variability and surface gravity waves do not show any significant diurnal variability. Richardson number estimates show that, on average, wind-induced shear is not strong enough to erode the strength of water column stratification within the upwelling shadow.This is the publisher's version of record. The original submission is copyrighted by American Geophysical Union and can be found here: http://www.agu.org

Probing complex RNA structures by mechanical force

RNA secondary structures of increasing complexity are probed combining single molecule stretching experiments and stochastic unfolding/refolding simulations. We find that force-induced unfolding pathways cannot usually be interpretated by solely invoking successive openings of native helices. Indeed, typical force-extension responses of complex RNA molecules are largely shaped by stretching-induced, long-lived intermediates including non-native helices. This is first shown for a set of generic structural motifs found in larger RNA structures, and then for Escherichia coli's 1540-base long 16S ribosomal RNA, which exhibits a surprisingly well-structured and reproducible unfolding pathway under mechanical stretching. Using out-of-equilibrium stochastic simulations, we demonstrate that these experimental results reflect the slow relaxation of RNA structural rearrangements. Hence, micromanipulations of single RNA molecules probe both their native structures and long-lived intermediates, so-called "kinetic traps", thereby capturing -at the single molecular level- the hallmark of RNA folding/unfolding dynamics.Comment: 9 pages, 9 figure

Analysis of all available zodiacal light observations Final report

Interplanetary dust model ensemble derived for analysis of zodiacal light observation

Plasmas and Controlled Nuclear Fusion

Contains reports on thirteen research projects split into two sections.National Science Foundation (Grant GK-57)National Science Foundation (Grant GK-614

Genetic and transcriptomic analysis of transcription factor genes in the model halophilic Archaeon: coordinate action of TbpD and TfbA

<p>Abstract</p> <p>Background</p> <p>Archaea are prokaryotic organisms with simplified versions of eukaryotic transcription systems. Genes coding for the general transcription factors TBP and TFB are present in multiple copies in several Archaea, including <it>Halobacterium </it>sp. NRC-1. Multiple TBP and TFBs have been proposed to participate in transcription of genes via recognition and recruitment of RNA polymerase to different classes of promoters.</p> <p>Results</p> <p>We attempted to knock out all six TBP and seven TFB genes in <it>Halobacterium </it>sp. NRC-1 using the <it>ura</it>3-based gene deletion system. Knockouts were obtained for six out of thirteen genes, <it>tbp</it>CDF and <it>tfb</it>ACG, indicating that they are not essential for cell viability under standard conditions. Screening of a population of 1,000 candidate mutants showed that genes which did not yield mutants contained less that 0.1% knockouts, strongly suggesting that they are essential. The transcriptomes of two mutants, Δ<it>tbp</it>D and Δ<it>tfb</it>A, were compared to the parental strain and showed coordinate down regulation of many genes. Over 500 out of 2,677 total genes were regulated in the Δ<it>tbp</it>D and Δ<it>tfb</it>A mutants with 363 regulated in both, indicating that over 10% of genes in both strains require the action of both TbpD and TfbA for normal transcription. Culturing studies on the Δ<it>tbp</it>D and Δ<it>tfb</it>A mutant strains showed them to grow more slowly than the wild-type at an elevated temperature, 49°C, and they showed reduced viability at 56°C, suggesting TbpD and TfbA are involved in the heat shock response. Alignment of TBP and TFB protein sequences suggested the expansion of the TBP gene family, especially in <it>Halobacterium </it>sp. NRC-1, and TFB gene family in representatives of five different genera of haloarchaea in which genome sequences are available.</p> <p>Conclusion</p> <p>Six of thirteen TBP and TFB genes of <it>Halobacterium </it>sp. NRC-1 are non-essential under standard growth conditions. TbpD and TfbA coordinate the expression of over 10% of the genes in the NRC-1 genome. The Δ<it>tbp</it>D and Δ<it>tfb</it>A mutant strains are temperature sensitive, possibly as a result of down regulation of heat shock genes. Sequence alignments suggest the existence of several families of TBP and TFB transcription factors in <it>Halobacterium </it>which may function in transcription of different classes of genes.</p