5,074 research outputs found
So near and yet so far: harmonic radar reveals reduced homing ability of Nosema infected honeybees.
© 2014 Wolf et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted
use, distribution, and reproduction in any medium, provided the original author and source are credited.Citation: Wolf S, McMahon DP, Lim KS, Pull CD, Clark SJ, et al. (2014) So Near and Yet So Far: Harmonic Radar Reveals Reduced Homing Ability of Nosema Infected
Honeybees. PLoS ONE 9(8): e103989. doi:10.1371/journal.pone.0103989Pathogens may gain a fitness advantage through manipulation of the behaviour of their hosts. Likewise, host behavioural changes can be a defence mechanism, counteracting the impact of pathogens on host fitness. We apply harmonic radar technology to characterize the impact of an emerging pathogen--Nosema ceranae (Microsporidia)--on honeybee (Apis mellifera) flight and orientation performance in the field. Honeybees are the most important commercial pollinators. Emerging diseases have been proposed to play a prominent role in colony decline, partly through sub-lethal behavioural manipulation of their hosts. We found that homing success was significantly reduced in diseased (65.8%) versus healthy foragers (92.5%). Although lost bees had significantly reduced continuous flight times and prolonged resting times, other flight characteristics and navigational abilities showed no significant difference between infected and non-infected bees. Our results suggest that infected bees express normal flight characteristics but are constrained in their homing ability, potentially compromising the colony by reducing its resource inputs, but also counteracting the intra-colony spread of infection. We provide the first high-resolution analysis of sub-lethal effects of an emerging disease on insect flight behaviour. The potential causes and the implications for both host and parasite are discussed.Biotechnology and Biological Sciences Research Council (BBSRC)Department for Environment Food & Rural Affairs (DEFRA)Natural Environment Research Council (NERC)The Scottish GovernmentWellcome Trus
de Sitter symmetry of Neveu-Schwarz spinors
We study the relations between Dirac fields living on the 2-dimensional
Lorentzian cylinder and the ones living on the double-covering of the
2-dimensional de Sitter manifold, here identified as a certain coset space of
the group . We show that there is an extended notion of de Sitter
covariance only for Dirac fields having the Neveu-Schwarz anti-periodicity and
construct the relevant cocycle. Finally, we show that the de Sitter symmetry is
naturally inherited by the Neveu-Schwarz massless Dirac field on the cylinder.Comment: 24 page
Flight performance of actively foraging honey bees is reduced by a common pathogen
ArticleSudden and severe declines in honey bee (Apis mellifera) colony health in the US and Europe have been attributed, in part, to emergent microbial pathogens, however, the mechanisms behind the impact are unclear. Using roundabout flight mills, we measured the flight distance and duration of actively foraging, healthy-looking honey bees sampled from standard colonies, before quantifying the level of infection by Nosema ceranae and Deformed Wing Virus complex (DWV) for each bee. Neither the presence nor quantity of N. ceranae at low, natural levels of infection had any effect on flight distance or duration, but presence of DWV reduced flight distance by two thirds and duration by one half. Quantity of DWV was shown to have a significant, but weakly positive relation with flight distance and duration, however, the low amount of variation that was accounted for suggests further investigation by dose-response assays is required. We conclude that widespread, naturally occurring levels of infection by DWV weaken the flight ability of honey bees and high levels of within-colony prevalence are likely to reduce efficiency and increase the cost of resource acquisition. Predictions of implications of pathogens on colony health and function should take account of sub-lethal effects on flight performance.This work was funded by the Insect Pollinators Initiative (IPI) grants BB/I000100/1,
BB/I000097/1 and BB/I000097/2, C.B. Dennis British Beekeepers' Research Trust and the
High Wycombe Beekeepers’ Association. The IPI is funded jointly by the BBSRC, Defra,
NERC, The Scottish Government and The Wellcome Trust, under the LWEC Partnership.
Rothamsted Research is a national institute of bioscience strategically funded by the BBSRC
The RING-CH ligase K5 antagonizes restriction of KSHV and HIV-1 particle release by mediating ubiquitin-dependent endosomal degradation of tetherin
Tetherin (CD317/BST2) is an interferon-induced membrane protein that inhibits the release of diverse enveloped viral particles. Several mammalian viruses have evolved countermeasures that inactivate tetherin, with the prototype being the HIV-1 Vpu protein. Here we show that the human herpesvirus Kaposi's sarcoma-associated herpesvirus (KSHV) is sensitive to tetherin restriction and its activity is counteracted by the KSHV encoded RING-CH E3 ubiquitin ligase K5. Tetherin expression in KSHV-infected cells inhibits viral particle release, as does depletion of K5 protein using RNA interference. K5 induces a species-specific downregulation of human tetherin from the cell surface followed by its endosomal degradation. We show that K5 targets a single lysine (K18) in the cytoplasmic tail of tetherin for ubiquitination, leading to relocalization of tetherin to CD63-positive endosomal compartments. Tetherin degradation is dependent on ESCRT-mediated endosomal sorting, but does not require a tyrosine-based sorting signal in the tetherin cytoplasmic tail. Importantly, we also show that the ability of K5 to substitute for Vpu in HIV-1 release is entirely dependent on K18 and the RING-CH domain of K5. By contrast, while Vpu induces ubiquitination of tetherin cytoplasmic tail lysine residues, mutation of these positions has no effect on its antagonism of tetherin function, and residual tetherin is associated with the trans-Golgi network (TGN) in Vpu-expressing cells. Taken together our results demonstrate that K5 is a mechanistically distinct viral countermeasure to tetherin-mediated restriction, and that herpesvirus particle release is sensitive to this mode of antiviral inhibition
Aryl hydrocarbon receptor is required for optimal B-cell proliferation
The aryl hydrocarbon receptor (AhR), a transcription factor known for mediating xenobiotic toxicity, is expressed in B cells, which are known targets for environmental pollutants. However, it is unclear what the physiological functions of AhR in B cells are. We show here that expression of Ahr in B cells is up‐regulated upon B‐cell receptor (BCR) engagement and IL‐4 treatment. Addition of a natural ligand of AhR, FICZ, induces AhR translocation to the nucleus and transcription of the AhR target gene Cyp1a1, showing that the AhR pathway is functional in B cells. AhR‐deficient (Ahr (−/−)) B cells proliferate less than AhR‐sufficient (Ahr (+/+)) cells following in vitro BCR stimulation and in vivo adoptive transfer models confirmed that Ahr (−/−) B cells are outcompeted by Ahr (+/+) cells. Transcriptome comparison of AhR‐deficient and AhR‐sufficient B cells identified cyclin O (Ccno), a direct target of AhR, as a top candidate affected by AhR deficiency
Electric Field Control of Spin Transport
Spintronics is an approach to electronics in which the spin of the electrons
is exploited to control the electric resistance R of devices. One basic
building block is the spin-valve, which is formed if two ferromagnetic
electrodes are separated by a thin tunneling barrier. In such devices, R
depends on the orientation of the magnetisation of the electrodes. It is
usually larger in the antiparallel than in the parallel configuration. The
relative difference of R, the so-called magneto-resistance (MR), is then
positive. Common devices, such as the giant magneto-resistance sensor used in
reading heads of hard disks, are based on this phenomenon. The MR may become
anomalous (negative), if the transmission probability of electrons through the
device is spin or energy dependent. This offers a route to the realisation of
gate-tunable MR devices, because transmission probabilities can readily be
tuned in many devices with an electrical gate signal. Such devices have,
however, been elusive so far. We report here on a pronounced gate-field
controlled MR in devices made from carbon nanotubes with ferromagnetic
contacts. Both the amplitude and the sign of the MR are tunable with the gate
voltage in a predictable manner. We emphasise that this spin-field effect is
not restricted to carbon nanotubes but constitutes a generic effect which can
in principle be exploited in all resonant tunneling devices.Comment: 22 pages, 5 figure
Orientation in high-flying migrant insects in relation to flows: mechanisms and strategies
High-flying insect migrants have been shown to display sophisticated flight orientations that can, for example, maximize distance travelled by exploiting tailwinds, and reduce drift from seasonally optimal directions. Here, we provide a comprehensive overview of the theoretical and empirical evidence for the mechanisms underlying the selection and maintenance of the observed flight headings, and the detection of wind direction and speed, for insects flying hundreds of metres above the ground. Different mechanisms may be used—visual perception of the apparent ground movement or mechanosensory cues maintained by intrinsic features of the wind—depending on circumstances (e.g. day or night migrations). In addition to putative turbulence-induced velocity, acceleration and temperature cues, we present a new mathematical analysis which shows that 'jerks' (the time-derivative of accelerations) can provide indicators of wind direction at altitude. The adaptive benefits of the different orientation strategies are briefly discussed, and we place these new findings for insects within a wider context by comparisons with the latest research on other flying and swimming organisms
Probing the close environment of young stellar objects with interferometry
The study of Young Stellar Objects (YSOs) is one of the most exciting topics
that can be undertaken by long baseline optical interferometry. The magnitudes
of these objects are at the edge of capabilities of current optical
interferometers, limiting the studies to a few dozen, but are well within the
capability of coming large aperture interferometers like the VLT
Interferometer, the Keck Interferometer, the Large Binocular Telescope or
'OHANA. The milli-arcsecond spatial resolution reached by interferometry probes
the very close environment of young stars, down to a tenth of an astronomical
unit. In this paper, I review the different aspects of star formation that can
be tackled by interferometry: circumstellar disks, multiplicity, jets. I
present recent observations performed with operational infrared
interferometers, IOTA, PTI and ISI, and I show why in the next future one will
extend these studies with large aperture interferometers.Comment: Review to be published in JENAM'2002 proceedings "The Very Large
Telescope Interferometer Challenges for the future
Feasibility of TEE-guided stroke risk assessment in atrial fibrillation—background, aims, design and baseline data of the TIARA pilot study
Contains fulltext :
97916.pdf (publisher's version ) (Open Access)BACKGROUND: Antithrombotic management in atrial fibrillation (AF) is currently based on clinical characteristics, despite evidence of potential fine-tuning with transoesophageal echocardiography (TEE). This open, randomised, multicentre study addresses the hypothesis that a comprehensive strategy of TEE-based aspirin treatment in AF patients is feasible and safe. METHODS: Between 2005 and 2009, ten large hospitals in the Netherlands enrolled AF patients with a moderate risk of stroke. Patients without thrombogenic TEE characteristics were randomised to aspirin or vitamin K antagonists (VKA). The primary objective is to show that TEE-based aspirin treatment is safe compared with VKA therapy. The secondary objective tests feasibility of TEE as a tool to detect echocardiographic features of high stroke risk. This report compares randomised to non-randomised patients and describes the feasibility of a TEE-based approach. RESULTS: In total, 310 patients were included. Sixty-nine patients were not randomised because of non-visualisation (n = 6) or TEE risk factors (n = 63). Compared with non-randomised patients, randomised patients (n = 241) were younger (65 +/- 11 vs. 69 +/- 9 years, p = 0.004), had less coronary artery disease (9 vs. 20%, p = 0.018), previous TIA (1.7 vs. 7.2%, p = 0.029), AF during TEE (25 vs. 54%, p < 0.001), mitral incompetence (55 vs. 70%, p = 0.038), VKA use (69 vs. 82%, p = 0.032), had a lower mean CHADS(2) score (1.2 +/- 0.6 vs. 1.6 +/- 1.0, p = 0.004), and left ventricular ejection fraction (59 +/- 8 vs. 56 +/- 8%, p = 0.016). CONCLUSIONS: This study shows that a TEE-based approach for fine-tuning stroke risk in AF patients with a moderate risk for stroke is feasible. Follow-up data will address the safety of this TEE-based approach
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