Mechanism of Water Dynamics in Hyaluronic Dermal Fillers Revealed by Nuclear Magnetic Resonance Relaxometry

This project has received funding from the European Union’s Horizon 2020 research and innovation programme under project “IDentIFY”, grant agreement No 668119.Peer reviewedPublisher PD

Slow dynamics of solid proteins : Nuclear Magnetic Resonance relaxometry versus Dielectric Spectroscopy

Acknowledgement This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 668119 (project “IDentIFY”).Peer reviewedPublisher PD

Dynamics of Ionic Liquids in Confinement by Means of NMR Relaxometry—EMIM-FSI in a Silica Matrix as an Example

1H and 19F spin–lattice relaxation studies for 1-ethyl-3-methylimidazolium bis(fluorosulfonyl)imide in bulk and mesoporous MCM-41 silica matrix confinement were performed under varying temperatures in a broad range of magnetic fields, corresponding to 1H resonance frequency from 5Hz to 30MHz.A thorough analysis of the relaxation data revealed a three-dimensional translation diffusion of the ions in the bulk liquid and two-dimensional diffusion in the vicinity of the confining walls in the confinement. Parameters describing the translation dynamics were determined and compared. The rotational motion of both kinds of ions in the confinement was described by two correlation times that might be attributed to anisotropic reorientation of these species

Water Dynamics in Whey-Protein-Based Composite Hydrogels by Means of NMR Relaxometry

Whey-protein-isolate-based composite hydrogels with encapsulated black carrot (Daucus carota) extract were prepared by heat-induced gelation. The hydrogels were blended with gum tragacanth, pectin and xanthan gum polysaccharides for modulating their properties. H-1 spin-lattice relaxation experiments were performed in a broad frequency range, from 4 kHz to 30 MHz, to obtain insight into the influence of the different polysaccharides and of the presence of black carrot on dynamical properties of water molecules in the hydrogel network. The H-1 spin-lattice relaxation data were decomposed into relaxation contributions associated with confined and free water fractions. The population of the confined water fraction and the value of the translation diffusion coefficient of water molecules in the vicinity of the macromolecular network were quantitatively determined on the basis of the relaxation data. Moreover, it was demonstrated that the translation diffusion is highly anisotropic (two-dimensional, 2D)

Data from: Prognostic and clinical significance of miRNA-205 in endometrioid endometrial cancer

Endometrial cancer is one of the most common malignancies of the reproductive female tract, with endometrioid endometrial cancer being the most frequent type. Despite the relatively favourable prognosis in cases of endometrial cancer, there is a necessity to evaluate clinical and prognostic utility of new molecular markers. MiRNAs are small, non-coding RNA molecules that take part in RNA silencing and post-transcriptional regulation of gene expression. Altered expression of miRNAs may be associated with cancer initiation, progression and metastatic capabilities. MiRNA-205 seems to be one of the key regulators of gene expression in endometrial cancer. In this study, we investigated clinical and prognostic role of miRNA-205 in endometrioid endometrial cancer. After total RNA extraction from 100 archival formalin-fixed paraffin-embedded tissues, real-time quantitative RT-PCR was used to define miRNA-205 expression levels. The aim of the study was to evaluate miRNA-205 expression levels in regard to patients’ clinical and histopathological features, such as: survival rate, recurrence rate, staging, myometrial invasion, grading and lymph nodes involvement. Higher levels of miRNA-205 expression were observed in tumours with less than half of myometrial invasion and non-advanced cancers. Kaplan-Maier analysis revealed that higher levels of miRNA-205 were associated with better overall survival (p = 0,034). These results indicate potential clinical utility of miRNA-205 as a prognostic marker

RQ miR205 dryad

Files include RAW data on qRT-PCR (Ct values and RQ values) and, additionally, basic clinical data are provided (the summary of clinical data is presented in the article)

Symmetry induced structuring of ultrathin FeO and Fe3O4 films on Pt 111 and Ru 0001

Iron oxide films epitaxially grown on close-packed metal single crystal substrates exhibit nearly-perfect structural order, high catalytic activity (FeO) and room-temperature magnetism (Fe3O4). However, the morphology of the films, especially in the ultrathin regime, can be significantly influenced by the crystalline structure of the used support. This work reports an ultra-high vacuum (UHV) low energy electron/synchrotron light-based X-ray photoemission electron microscopy (LEEM/XPEEM) and electron diffraction (µLEED) study of the growth of FeO and Fe3O4 on two closed-packed metal single crystal surfaces: Pt(111) and Ru(0001). The results reveal the influence of the mutual orientation of adjacent substrate terraces on the morphology of iron oxide films epitaxially grown on top of them. On fcc Pt(111), which has the same mutual orientation of adjacent monoatomic terraces, FeO(111) grows with the same in-plane orientation on all substrate terraces. For Fe3O4(111), one or two orientations are observed depending on the growth conditions. On hcp Ru(0001), the adjacent terraces of which are ‘rotated’ by 180° with respect to each other, the in-plane orientation of initial FeO(111) and Fe3O4(111) crystallites is determined by the orientation of the substrate terrace on which they nucleated. The adaptation of three-fold symmetric iron oxides to three-fold symmetric substrate terraces leads to natural structuring of iron oxide films, i.e., the formation of patch-like magnetite layers on Pt(111) and stripe-like FeO and Fe3O4 structures on Ru(0001)

Pathological characteristics.

<p>Pathological characteristics.</p

MiRNA-205 expression in terms of grading: G1 vs. G2 vs. G3 (Kruskal–Wallis test, G2 vs. G3, p = 0.02).

<p>MiRNA-205 expression in terms of grading: G1 vs. G2 vs. G3 (Kruskal–Wallis test, G2 vs. G3, p = 0.02).</p

MiRNA-205 expression: I, II vs. III, IV FIGO stage (p = 0,045).

<p>MiRNA-205 expression: I, II vs. III, IV FIGO stage (p = 0,045).</p