Inhibition and comprehensive analysis of hiv-1 vpu, examination of retroviral trafficking, and characterization of sars-cov-2 genetic material in wastewater

Both HIV-1 and SARS-CoV-2 have been responsible for millions of infectionrelated deaths. Despite advances in treatment for both viruses, neither has a cure, and those living with the disease can unknowingly spread the virus. Because of this, research efforts to further the understanding, and control of both HIV-1 and SARS-CoV-2 are of upmost importance. A potential target for additional treatments for HIV -1 is Vpu, an accessory protein. Vpu counteracts host proteins that are detrimental to the virus, such as CD4 and Tetherin, enhancing viral dissemination and evasion of the host immune system. We have generated a library of Vpu clones in which each codon was individually randomized, resulting in a possible 1,620 amino acid mutants. With this library, we are able to look Vpu : target interactions in a comprehensive manner increasing our understanding of important residues in the functional protein. In a similar manner, we used a known target of Vpu, GaLV Env, to create a high throughput screening method for Vpu inhibitors and successfully identified two inhibitors with Vpu specificity that rescued CD4 and Tetherin downmodulation while also rescuing ADCC killing of infectious cells. In another retroviral study, examining pseudotype compatibility between various retroviruses and glycoproteins, we identified a mis-trafficking event within assembly of MLV gag protein inside HeLa but not HEK293FT cells. This suggests that the viral particles are mis-trafficked, and are either surrounded by or directly adjacent to lysosomal particles. In 2020, SARS-CoV-2 became a global pandemic, and many research efforts transitioned to finding treatments, vaccines, cures, and methods of understanding and tracking viral spread. It was soon noted that SARS-CoV-2 genetic material was detectable in wastewater. Here, we characterize genetic material collected from wastewater samples and find that wastewater likely contains fully intact enveloped particles. Taken together, these 4 studies contribute to a better understanding of HIV-1 and SARS-CoV-2 and provide tools that can be used long-term to further the understanding of these viruses

Effects of perceived racial discrimination on health status and health behavior: A differential mediation hypothesis

Objective: Prospective data tested a “differential mediation” hypothesis: The relations (found in previous research) between perceived racial discrimination and physical health status versus health-impairing behavior (problematic substance use) are mediated by two different types of affective reactions, internalizing and externalizing. Method: The sample included 680 African American women from the Family and Community Health Study (M age = 37 years at Time 1; 45 years at Time 4). Four waves of data were analyzed. Perceived discrimination was assessed, along with anxiety and depression (internalizing) and hostility/anger (externalizing) as mediators, and physical health status and problematic substance use (drinking) as outcomes. Results: Structural equation modeling indicated that discrimination predicted increases in both externalizing and internalizing reactions. These affective responses, in turn, predicted subsequent problematic substance use and physical health status, respectively, also controlling for earlier reports. In each case, the indirect effects from discrimination through the affective mediator to the specific health outcome were significant and consistent with the differential mediation hypothesis. Conclusions: Perceived racial discrimination is associated with increases in internalizing and externalizing reactions among Black women, but these reactions are related to different health outcomes. Changes in internalizing are associated with self-reported changes in physical health status, whereas changes in externalizing are associated with changes in substance use problems. Discussion focuses on the processes whereby discrimination affects health behavior and physical health status

Blueberry Advisory Committee Research Report

The 1985 edition of the Blueberry Progress Reports was prepared for the Maine Blueberry Commission and the University of Maine Blueberry Advisory Committee by researchers with the Maine Agricultural Experiment Station and Maine Cooperative Extension Service at the University of Maine, Orono. Projects in this report include: 1. Control of secondary blueberry pest insects 2. Effect of pruning practices on blueberry insect abundance 3. Effect of hexazinone on species distribution in lowbush blueberry fields 4. Dichlobenil for control of bunchberry 5. Evaluation of postemergence herbicides for grass control 6. Hand-wiper applications of herbicides on woody weeds 7. Evaluation of steam as a pruning practice for lowbush blueberry fields 8. Evaluation of glyphosate and 2,4-D applied with a commercial weed roller to control woody weeds 9. Hand-wiping and cutting treatments for dogbane 9. Evaluation of airblast-sprayer application of asulam for bracken fern control 10. Spot treatment of woody weeds with 2,4-D in oil 11. Chemical control of Botrytis blossom blight 12. Evaluation of preliminary steam treatments (1984) at Blueberry Hill Farm 13. Long term effects of N and NPK fertilizer on plant growth and yield 14. Nutritional survey of selected lowbush blueberry fields 15. Interaction of fertility and pruning practices on soil characteristics and lowbush blueberry growth and yield 16. Frequency of fertility application for establishment of lowbush blueberry seedlings 17. Slow release vs. liquid fertilizer for establishment of lowbush blueberry seedlings 18. The effect of several mulches on frost heaving, soil moisture, soil temperature and rhizome development 19. Blueberry product development: raisin-type blueberries 20. Blueberry product development: blueberry puree 21. Addendum: 1985 Report from Moody Trevet

Operation 'Cerberus Action' and the 'Four Corners' Prosecution.

There is a generally accepted belief that a well publicised prosecution, which results in the conviction of the offenders will deter crime by sending out a ‘clear message’ to those intending to offend. Those who seek to enforce the legal protection of antiquities and archaeological sites will often decry the number of prosecutions brought, and urge a more aggressive prosecution policy against looters and traffickers in antiquities. However a prosecution may not always produce the anticipated outcome of deterrence. In this article a lawyer examines a recent high profile operation undertaken by the Federal Bureau of Investigation and the Bureau of Land Management against looters and traffickers in the south west of the United States for breaches of the Archaeological Resources Protection Act of 1979 and its outcome. It will begin with a short consideration of the context in which the prosecutions were brought: the scale of looting in the area; the difficulties facing those who have to enforce the law; the legal and historical background, and the belief of many in the area that they have a right to dig for artefacts and to collect or sell them. It will then consider ‘Operation Cerberus Action’ and its consequences in some detail, drawing on contemporaneous newspaper accounts and blog comments to illustrate that a prosecution, even where it results in conviction of all the defendants, may be counterproductive, serving only to entrench existing attitudes rather than encouraging behavioural change in intending looters and traffickers

Film remakes, the black sheep of translation

Film remakes have often been neglected by translation studies in favour of other forms of audiovisual translation such as subtitling and dubbing. Yet, as this article will argue, remakes are also a form of cinematic translation. Beginning with a survey of previous, ambivalent approaches to the status of remakes, it proposes that remakes are multimodal, adaptive translations: they translate the many modes of the film being remade and offer a reworking of that source text. The multimodal nature of remakes is explored through a reading of Breathless, Jim McBride's 1983 remake of Jean-Luc Godard's À bout de souffle (1959), which shows how remade films may repeat the narrative of, but differ on multiple levels from, their source films. Due to the collaborative nature of film production, remakes involve multiple agents of translation. As such, remakes offer an expanded understanding of audiovisual translation

Multiple novel prostate cancer susceptibility signals identified by fine-mapping of known risk loci among Europeans

Genome-wide association studies (GWAS) have identified numerous common prostate cancer (PrCa) susceptibility loci. We have fine-mapped 64 GWAS regions known at the conclusion of the iCOGS study using large-scale genotyping and imputation in 25 723 PrCa cases and 26 274 controls of European ancestry. We detected evidence for multiple independent signals at 16 regions, 12 of which contained additional newly identified significant associations. A single signal comprising a spectrum of correlated variation was observed at 39 regions; 35 of which are now described by a novel more significantly associated lead SNP, while the originally reported variant remained as the lead SNP only in 4 regions. We also confirmed two association signals in Europeans that had been previously reported only in East-Asian GWAS. Based on statistical evidence and linkage disequilibrium (LD) structure, we have curated and narrowed down the list of the most likely candidate causal variants for each region. Functional annotation using data from ENCODE filtered for PrCa cell lines and eQTL analysis demonstrated significant enrichment for overlap with bio-features within this set. By incorporating the novel risk variants identified here alongside the refined data for existing association signals, we estimate that these loci now explain ∼38.9% of the familial relative risk of PrCa, an 8.9% improvement over the previously reported GWAS tag SNPs. This suggests that a significant fraction of the heritability of PrCa may have been hidden during the discovery phase of GWAS, in particular due to the presence of multiple independent signals within the same regio

Effects of perceived racial discrimination on health status and health behavior: A differential mediation hypothesis

Objective: Prospective data tested a “differential mediation” hypothesis: The relations (found in previous research) between perceived racial discrimination and physical health status versus health-impairing behavior (problematic substance use) are mediated by two different types of affective reactions, internalizing and externalizing. Method: The sample included 680 African American women from the Family and Community Health Study (M age = 37 years at Time 1; 45 years at Time 4). Four waves of data were analyzed. Perceived discrimination was assessed, along with anxiety and depression (internalizing) and hostility/anger (externalizing) as mediators, and physical health status and problematic substance use (drinking) as outcomes. Results: Structural equation modeling indicated that discrimination predicted increases in both externalizing and internalizing reactions. These affective responses, in turn, predicted subsequent problematic substance use and physical health status, respectively, also controlling for earlier reports. In each case, the indirect effects from discrimination through the affective mediator to the specific health outcome were significant and consistent with the differential mediation hypothesis. Conclusions: Perceived racial discrimination is associated with increases in internalizing and externalizing reactions among Black women, but these reactions are related to different health outcomes. Changes in internalizing are associated with self-reported changes in physical health status, whereas changes in externalizing are associated with changes in substance use problems. Discussion focuses on the processes whereby discrimination affects health behavior and physical health status.This is an author's manuscript of an article from Health Psychology 33 (2014): 11–19, doi:10.1037/a0033857.This article may not exactly replicate the final version published in the APA journal. It is not the copy of record.</p