Pharmacogenetics of OATP Transporters Reveals That SLCO1B1 c.388A>G Variant Is Determinant of Increased Atorvastatin Response

Aims: The relationship between variants in SLCO1B1 and SLCO2B1 genes and lipid-lowering response to atorvastatin was investigated. Material and Methods: One-hundred-thirty-six unrelated individuals with hypercholesterolemia were selected and treated with atorvastatin (10 mg/day/4 weeks). They were genotyped with a panel of ancestry informative markers for individual African component of ancestry (ACA) estimation by SNaPshot® and SLCO1B1 (c.388A>G, c.463C>A and c.521T>C) and SLCO2B1 (−71T>C) gene polymorphisms were identified by TaqMan® Real-time PCR. Results: Subjects carrying SLCO1B1 c.388GG genotype exhibited significantly high low-density lipoprotein (LDL) cholesterol reduction relative to c.388AA+c.388AG carriers (41 vs. 37%, p = 0.034). Haplotype analysis revealed that homozygous of SLCO1B1*15 (c.521C and c.388G) variant had similar response to statin relative to heterozygous and non-carriers. A multivariate logistic regression analysis confirmed that c.388GG genotype was associated with higher LDL cholesterol reduction in the study population (OR: 3.2, CI95%:1.3–8.0, p < 0.05). Conclusion: SLCO1B1 c.388A>G polymorphism causes significant increase in atorvastatin response and may be an important marker for predicting efficacy of lipid-lowering therapy

AVALIAÇÃO DO REPLANTIO DA VEGETAÇÃO DO MANGUEZAL NO SACO DA FAZENDA, ITAJAÍ(SC)

In this work the survival and performance of transplanted mangrove trees in Saco da Fazenda (Itajaí, SC), were monitored from March to December 2001. Three hundred and fifty transplanted trees were tagged and the trunk basal diameter and tree height were measured. The health of trunk, leaves and roots were also observed. Maximum tree heights were about 3.5m and larger trunk diameters were about 4.0-4.5cm. Mortality was higher to trees higher than 1.0m. Trees higher than 3.0m did not survive to replenishment. Survival of high trees occurred only in areas protected from hydrodynamics, were stress by sand mobility were lower.O presente estudo compreende as atividades de marcação e monitoramento do processo de replantio da vegetação do manguezal que coloniza a orla do Saco da Fazenda, Itajaí (SC), correspondendo às etapas desenvolvidas no período de março a dezembro de 2001. A metodologia consistiu na marcação das árvores e na observação do diâmetro basal e altura de cada indivíduo, bem como no estado geral do caule, folhas e raízes. Nesse período de estudo foram etiquetados 350 indivíduos. As alturas máximas observadas raramente ultrapassam 3,5 m e os maiores diâmetros encontram-se na faixa de 4,0 a 4,5 cm. O maior índice de mortos ocorreu no intervalo de altura superior a 1,0 m e com diâmetros entre 1,0 a 2,0 cm. Indivíduos com altura superior a 3,0 m apresentaram 100% de mortalidade. Indivíduos com maior altura que permaneceram vivos, encontrava- se nas porções mais internas do bosque transplantado, pois nessa área é menor o estresse mecânico provocado pela inundação de maré