A coupled calibration and modelling approach to the understanding of dry-land lake oxygen isotope records

Comparisons between climate proxies and instrumental records through the last two centuries are often used to understand better the controls on palaeoarchives and to find relationships that can be used to quantify changes in pre-instrumental climate. Here we compare an 80-year-long annually resolved oxygen isotope record from Nar Gölü, Turkey, a varved lake sequence, with instrumental records of temperature, precipitation, wind speed, relative humidity and calculated values of evaporation, all of which are known to be possible controls on lake oxygen isotope systems. Significant relationships are found between the isotope record and summer temperatures and evaporation suggesting these are dominant controls on the isotope hydrology of this non-outlet lake. Modelling the stable isotope hydrology of the lake system allows these relationships to be tested independently. We show that the isotope record follows the same trends in the temperature and evaporation records but that, even when combined, these two climatic factors cannot fully explain the magnitude of change observed in the isotope record. The models show the lake system is much less sensitive to changes in evaporation and temperature than the climate calibration suggests. Additional factors, including changes in the amount of precipitation, are required to amplify the isotope change. It is concluded that proxy-climate calibrations may incorrectly estimate the amplitude of past changes in individual climate parameters, unless validated independently

Bacterial nanocellulose production from naphthalene

Polycyclic aromatic compounds (PAHs) are toxic compounds that are released in the environment as a consequence of industrial activities. The restoration of PAH-polluted sites considers the use of bacteria capable of degrading aromatic compounds to carbon dioxide and water. Here we characterize a new Xanthobacteraceae strain, Starkeya sp. strain N1B, previously isolated during enrichment under microaerophilic conditions, which is capable of using naphthalene crystals as the sole carbon source. The strain produced a structured biofilm when grown on naphthalene crystals, which had the shape of a half-sphere organized over the crystal. Scanning electron microscopy (SEM) and GC-MS analysis indicated that the biofilm was essentially made of cellulose, composed of several micron-long nanofibrils of 60 nm diameter. A cellulosic biofilm was also formed when the cells grew with glucose as the carbon source. Fourier transformed infrared spectroscopy (FTIR) confirmed that the polymer was type I cellulose in both cases, although the crystallinity of the material greatly depended on the carbon source used for growth. Using genome mining and mutant analysis, we identified the genetic complements required for the transformation of naphthalene into cellulose, which seemed to have been successively acquired through horizontal gene transfer. The capacity to develop the biofilm around the crystal was found to be dispensable for growth when naphthalene was used as the carbon source, suggesting that the function of this structure is more intricate than initially thought. This is the first example of the use of toxic aromatic hydrocarbons as the carbon source for bacterial cellulose production. Application of this capacity would allow the remediation of a PAH into such a value-added polymer with multiple biotechnological usages.This work was supported by the European Regional Development Fund FEDER and grants from the Spanish Ministry of Economy and Competitiveness (BIO2017-82242-R)