Clique-Based Lower Bounds for Parsing Tree-Adjoining Grammars

up to lower order factors

λ4430 Emission by Comet Hyakutake

Comets provide a very different but well understood environment in which to search for DIBs. Observations of occultations by cometary comae have not detected DIBs, but none were very near the nucleus, where the column density of dust is highest. We report here unidentified emission bands, centered at λ4430, very near the nucleus of comet Hyakutake. These may be vaporized forms of grain carriers or fragments of large-molecule carriers. At least two different species appear to be present based on two different spatial distribution

Immune response of bovine milk somatic cells to endotoxin in healthy quarters with normal and very low cell counts

Low somatic cell count (SCC) is a reliable indicator of high-quality milk free of pathogenic microorganisms. Thus, an important goal in dairy practice is to produce milk with low SCC. Selection for cows with low SCC can sometimes lead to extremely low SCC in single quarters. The cells in milk are, however, predominantly immune cells with important immune functions. To investigate the mammary immune competence of quarters with very low SCC, healthy udder quarters of cows with normal SCC of (40-100)×103 cells/ml and very low SCC of <20×103 cells/ml were challenged with lipopolysaccharide (LPS) from Escherichia coli. In the first experiment, SCC and cell viability after a challenge with 50 ng of LPS/quarter was investigated. In the second experiment, tumour necrosis factor α (TNF-α) concentration and lactate dehydrogenase (LDH) activity in milk, and mRNA expression of various innate immune factors in milk cells were measured after a challenge with 100 μg LPS/quarter. LPS challenge induced an increase of SCC. SCC levels reached were higher in quarters with normal SCC and maximum SCC was reached 1 h earlier than in very low SCC quarters. The increase of TNF-α concentrations in milk in response to LPS challenge was lower in quarters with very low SCC than in quarters with normal SCC. The viability of cells and the LDH activity in milk increased in response to LPS challenge, however, without a difference between the groups. The mRNA expression of IL-1β and IL-8 was increased in milk cells at 12 h after LPS challenge, whereas that of TNF-α and lactoferrin was not increased at the measured time points (12, 24 and 36 h after LPS challenge). No differences of mRNA expression of measured immune factors between normal and very low SCC samples were detected. The study showed that udder quarters with very low SCC responded with a less marked increase of SCC compared with quarters with normal SCC. This difference corresponded with simultaneously lower TNF-α concentrations in milk. However, the immune competence of the cells themselves based on mRNA expression of TNF-α, IL-8, IL-1β, and lactoferrin, did not differ. The results may indicate that very low SCC can impair the immune competence of udder quarters, because the immune response in udder quarters with lower SCC is less efficient as fewer cells contribute to the production of immunoregulator

Prediction of total quarter milk somatic cell counts based on foremilk sampling

Determination of somatic cell count (SCC) is used worldwide in dairy practice to describe the hygienic status of the milk and the udder health of cows. When SCC is tested on a quarter level to detect single quarters with high SCC levels of cows for practical reasons, mostly foremilk samples after prestimulation (i.e. cleaning of the udder) are used. However, SCC is usually different in different milk fractions. Therefore, the goal of this study was the investigation of the use of foremilk samples for the estimation of total quarter SCC. A total of 378 milkings in 19 dairy cows were performed with a special milking device to drain quarter milk separately. Foremilk samples were taken after udder stimulation and before cluster attachment. SCC was measured in foremilk samples and in total quarter milk. Total quarter milk SCC could not be predicted precisely from foremilk SCC measurements. At relatively high foremilk SCC levels (>300×103 cells/ml) foremilk SCC were higher than total quarter milk. At around (50-300)×103 cells/ml foremilk and total quarter SCC did not differ considerably. Most interestingly, if foremilk SCC was lower than 50×103 cells/ml the total quarter SCC was higher than foremilk SCC. In addition, individual cows showed dramatic variations in foremilk SCC that were not very well related to total quarter milk SCC. In conclusion, foremilk samples are useful to detect high quarter milk SCC to recognize possibly infected quarters, only if precise cell counts are not required. However, foremilk samples can be deceptive if very low cell numbers are to be detecte

Cell population, viability, and some key immunomodulatory molecules in different milk somatic cell samples in dairy cows

Immune cells in the milk are most important in combating pathogens that invade the mammary gland. This study investigated the immune competence and viability of somatic milk cells that are already resident in milk and udders free of infection. Cells were studied in freshly removed milk to simulate conditions in the udder. Effects of incubation, cell preparation, and immunological stimulation with 0·5 μg/ml lipopolysaccharide (LPS) from Escherichia coli were analysed. Viability and differential counts of milk cells between high and low somatic cell count (SCC) quarters, and cisternal and alveolar milk with and without LPS stimulation were compared. Incubation and preparation of cells caused a cell loss which further increased with time independently of SCC and milk fraction. The viability of these cells was stable until 3 h post incubation and decreased until 6 h. Cell populations differed between both investigations, but did not change during the course of the experiment. mRNA expression of immune and apoptosis factors of the cells, measured by qPCR, did not change substantially: mRNA expression of caspase 3, Toll like receptor 4, and GM-CSF did not change, whereas the expression of the death receptor Fas/APO-1 (CD95), lactoferrin and lysozyme was decreased at 6 h. Cyclooxygenase-2 and TNF-α mRNA expression were decreased after 6 h of LPS treatment. In comparison with other studies in vivo or in vitro (in cell culture), in this study where cells are studied ex vivo (removed from the udder but kept in their natural environment, the milk) resident milk cells seem to be more vulnerable, less viable, less able to respond to stimulation, and thus less immune competent compared with cells that have freshly migrated from blood into milk after pathogen stimulation. The cell viability and differential cell count differed between high- and low-SCC milk and between cisternal and alveolar milk depending on the individual cow. In conclusion, the results support the view that for a most effective defence against invading pathogens the mammary gland is reliant on the recruitment of fresh immune cells from the bloo

Effects of an oral Echinacea purpurea alcoholic spissum extract in calves. A placebo-controlled, randomized, double blinded study

Background Antimicrobial resistance resulted in international accordance to strengthen the research to investigate novel treatment options including medicinal plants. To investigate the effect of two dosages of an orally hydro-ethanolic Echinacea purpurea (L.) MOENCH spissum extract (EP) on performance, clinical health, antibody production, red and white blood cell count and expression of immunological parameters in calves, a double-blinded placebo-controlled clinical trial has been performed

The inflammatory response of primary bovine mammary epithelial cells to Staphylococcus aureus strains is linked to the bacterial phenotype

Staphylococcus aureus is a major mastitis-causing pathogen in dairy cows. The latex agglutination-based Staphaurex test allows bovine S. aureus strains to be grouped into Staphaurex latex agglutination test (SLAT)-negative [SLAT(2)] and SLATpositive [SLAT(+)] isolates. Virulence and resistance gene profiles within SLAT(2) isolates are highly similar, but differ largely from those of SLAT(+) isolates. Notably, specific genetic changes in important virulence factors were detected in SLAT(2) isolates. Based on the molecular data, it is assumed that SLAT(+) strains are more virulent than SLAT(2) strains. The objective of this study was to investigate if SLAT(2) and SLAT(+) strains can differentially induce an immune response with regard to their adhesive capacity to epithelial cells in the mammary gland and in turn, could play a role in the course of mastitis. Primary bovine mammary epithelial cells (bMEC) were challenged with suspensions of heat inactivated SLAT(+) (n = 3) and SLAT(2) (n = 3) strains isolated from clinical bovine mastitis cases. After 1, 6, and 24 h, cells were harvested and mRNA expression of inflammatory mediators (TNF-a, IL-1b, IL-8, RANTES, SAA, lactoferrin, GM-CSF, COX-2, and TLR-2) was evaluated by reverse transcription and quantitative PCR. Transcription (DDCT) of most measured factors was induced in challenged bMEC for 6 and 24 h. Interestingly, relative mRNA levels were higher (P,0.05) in response to SLAT(+) compared to SLAT(2) strains. In addition, adhesion assays on bMEC also showed significant differences between SLAT(+) and SLAT(2) strains. The present study clearly shows that these two S. aureus strain types cause a differential immune response of bMEC and exhibit differences in their adhesion capacity in vitro. This could reflect differences in the severity of mastitis that the different strain types may induce

Counting patterns in strings and graphs

We study problems related to finding and counting patterns in strings and graphs. In the string-regime, we are interested in counting how many substring of a text are at Hamming (or edit) distance at most to a pattern . Among others, we are interested in the fully-compressed setting, where both and are given in a compressed representation. For both distance measures, we give the first algorithm that runs in (almost) linear time in the size of the compressed representations. We obtain the algorithms by new and tight structural insights into the solution structure of the problems. In the graph-regime, we study problems related to counting homomorphisms between graphs. In particular, we study the parameterized complexity of the problem #IndSub(), where we are to count all -vertex induced subgraphs of a graph that satisfy the property . Based on a theory of Lovász, Curticapean et al., we express #IndSub() as a linear combination of graph homomorphism numbers to obtain #W[1]-hardness and almost tight conditional lower bounds for properties that are monotone or that depend only on the number of edges of a graph. Thereby, we prove a conjecture by Jerrum and Meeks. In addition, we investigate the parameterized complexity of the problem #Hom(ℋ → ) for graph classes ℋ and . In particular, we show that for any problem in the class #W[1], there are classes ℋ_ and _ such that is equivalent to #Hom(ℋ_ → _ ).Wir untersuchen Probleme im Zusammenhang mit dem Finden und Zählen von Mustern in Strings und Graphen. Im Stringbereich ist die Aufgabe, alle Teilstrings eines Strings zu bestimmen, die eine Hamming- (oder Editier-)Distanz von höchstens zu einem Pattern haben. Unter anderem sind wir am voll-komprimierten Setting interessiert, in dem sowohl , als auch in komprimierter Form gegeben sind. Für beide Abstandsbegriffe entwickeln wir die ersten Algorithmen mit einer (fast) linearen Laufzeit in der Größe der komprimierten Darstellungen. Die Algorithmen nutzen neue strukturelle Einsichten in die Lösungsstruktur der Probleme. Im Graphenbereich betrachten wir Probleme im Zusammenhang mit dem Zählen von Homomorphismen zwischen Graphen. Im Besonderen betrachten wir das Problem #IndSub(), bei dem alle induzierten Subgraphen mit Knoten zu zählen sind, die die Eigenschaft haben. Basierend auf einer Theorie von Lovász, Curticapean, Dell, and Marx drücken wir #IndSub() als Linearkombination von Homomorphismen-Zahlen aus um #W[1]-Härte und fast scharfe konditionale untere Laufzeitschranken zu erhalten für , die monoton sind oder nur auf der Kantenanzahl der Graphen basieren. Somit beweisen wir eine Vermutung von Jerrum and Meeks. Weiterhin beschäftigen wir uns mit der Komplexität des Problems #Hom(ℋ → ) für Graphklassen ℋ und . Im Besonderen zeigen wir, dass es für jedes Problem in #W[1] Graphklassen ℋ_ und _ gibt, sodass äquivalent zu #Hom(ℋ_ → _ ) ist

The Effects of Using Word Sorts in Combination with iPad Spelling Applications on Spelling Acquisition of a Student with a Specific Learning Disability

This research studied the effects of using word sorts in combination with iPad spelling applications on the spelling acquisition of a third grade student with a Specific Learning Disability. The researcher measured the effects on spelling acquisition using pre- and post-assessments. The informal assessments used included a Words their Way Spelling assessment, a Nonsense word assessment from Teaching Phonics and Word Study in Intermediate Grades, and a questionnaire. The student engaged in word sorts prior to completing a spelling application on the iPad. The strategies implemented with gains in spelling skills and had a positive effect on the student\u27s attitude towards spelling. The research also showed positive effects in the student\u27s reading of nonsense words. More research applying these techniques to students with and without disabilities should be conducted to further explore these approaches