Tracing discourses of health and the body: exploring pre-service primary teachers\u27 constructions of `healthy\u27 bodies

Contemporary notions of childhood overweight and obesity have become increasingly influential in curriculum and pedagogy in school-based Health and Physical Education (HPE). Teachers\u27 delivery of HPE subject matter and related school practices are likely to have a considerable impact on the attitudes and beliefs of the children they teach, particularly in the primary school. It thus becomes important to consider the ways of thinking about and doing health (discourse positions on health) that teachers bring to their teaching of HPE. This paper examines pre-service teachers\u27 positions in relation to the health discourses to better understand what teachers, in this case beginning teachers, bring to their teaching of HPE and interactions with children in primary schools. It draws on a Foucauldian approach to discourse analysis to analyse pre-service teachers\u27 qualitative survey and interview responses to questions about meanings of health. Three key positions emerged, signifying Agreement, Disagreement and Negotiated positions in relation to the dominant discourses of health and the body

Robust detection of alternative splicing in a population of single cells

Single cell RNA-seq experiments provide valuable insight into cellular heterogeneity but suffer from low coverage, 3′ bias and technical noise. These unique properties of single cell RNA-seq data make study of alternative splicing difficult, and thus most single cell studies have restricted analysis of transcriptome variation to the gene level. To address these limitations, we developed SingleSplice, which uses a statistical model to detect genes whose isoform usage shows biological variation significantly exceeding technical noise in a population of single cells. Importantly, SingleSplice is tailored to the unique demands of single cell analysis, detecting isoform usage differences without attempting to infer expression levels for full-length transcripts. Using data from spike-in transcripts, we found that our approach detects variation in isoform usage among single cells with high sensitivity and specificity. We also applied SingleSplice to data from mouse embryonic stem cells and discovered a set of genes that show significant biological variation in isoform usage across the set of cells. A subset of these isoform differences are linked to cell cycle stage, suggesting a novel connection between alternative splicing and the cell cycle

SLICER: inferring branched, nonlinear cellular trajectories from single cell RNA-seq data

Accuracy of trajectory reconstruction using a subset of cells. (a) Graph showing how similar the SLICER trajectory is when computed using a random subset of lung cells. The blue bars show the similarity in cell ordering (units are percent sorted with respect to the trajectory constructed from all cells). The orange bars show the similarity in branch assignments (percentage of cells assigned to the same branch as the trajectory constructed from all cells). The values shown were obtained by averaging the results from five subsampled datasets for each percentage (80 %, 60 %, 40 %, and 20 %). (b) Order preservation and branch identity values computed as in panel (a), but for datasets sampled from the neural stem cell dataset. (PDF 106 kb

Experimental Investigation of Rotating Stall in a Research Multistage Axial Compressor

A collection of experimental data acquired in the NASA low-speed multistage axial compressor while operated in rotating stall is presented in this paper. The compressor was instrumented with high-response wall pressure modules and a static pressure disc probe for in-flow measurement, and a split-fiber probe for simultaneous measurements of velocity magnitude and flow direction. The data acquired to-date have indicated that a single fully developed stall cell rotates about the flow annulus at 50.6% of the rotor speed. The stall phenomenon is substantially periodic at a fixed frequency of 8.29 Hz. It was determined that the rotating stall cell extends throughout the entire compressor, primarily in the axial direction. Spanwise distributions of the instantaneous absolute flow angle, axial and tangential velocity components, and static pressure acquired behind the first rotor are presented in the form of contour plots to visualize different patterns in the outer (midspan to casing) and inner (hub to mid-span) flow annuli during rotating stall. In most of the cases observed, the rotating stall started with a single cell. On occasion, rotating stall started with two emerging stall cells. The root cause of the variable stall cell count is unknown, but is not attributed to operating procedures

The symbiosis of concurrency and verification: teaching and case studies

Concurrency is beginning to be accepted as a core knowledge area in the undergraduate CS curriculum—no longer isolated, for example, as a support mechanism in a module on operating systems or reserved as an advanced discipline for later study. Formal verification of system properties is often considered a difficult subject area, requiring significant mathematical knowledge and generally restricted to smaller systems employing sequential logic only. This paper presents materials, methods and experiences of teaching concurrency and verification as a unified subject, as early as possible in the curriculum, so that they become fundamental elements of our software engineering tool kit—to be used together every day as a matter of course. Concurrency and verification should live in symbiosis. Verification is essential for concurrent systems as testing becomes especially inadequate in the face of complex non-deterministic (and, therefore, hard to repeat) behaviours. Concurrency should simplify the expression of most scales and forms of computer system by reflecting the concurrency of the worlds in which they operate (and, therefore, have to model); simplified expression leads to simplified reasoning and, hence, verification. Our approach lets these skills be developed without requiring students to be trained in the underlying formal mathematics. Instead, we build on the work of those who have engineered that necessary mathematics into the concurrency models we use (CSP, ?-calculus), the model checker (FDR) that lets us explore and verify those systems, and the programming languages/libraries (occam-?, Go, JCSP, ProcessJ) that let us design and build efficient executable systems within these models. This paper introduces a workflow methodology for the development and verification of concurrent systems; it also presents and reflects on two open-ended case studies, using this workflow, developed at the authors’ two universities. Concerns analysed include safety (don’t do bad things), liveness (do good things) and low probability deadlock (that testing fails to discover). The necessary technical background is given to make this paper self-contained and its work simple to reproduce and extend

Modulation of aggression in male mice: influence of group size and cage size

Aggression in group-housed male mice is known to be influenced by both cage size and group size. However, the interdependency of these two parameters has not been studied yet. In this study, the level of aggression in groups of three, five, or eight male BALB/c mice housed in cages with a floor size of either 80 or 125 cm2/animal was estimated weekly after cage cleaning for a period of 14 weeks. Furthermore, urine corticosterone levels, food and water intake, body weight, and number of wounds were measured weekly. At the end of the experiment, tyrosine hydroxylase (TH) activity, testosterone levels, and weight of spleen, thymus, testes, and seminal vesicles were determined. Results indicate a moderate increase of intermale aggression in larger cages when compared to the smaller cages. Aggression in groups of eight animals was considerably higher than in groups of three animals. The increase of agonistic behavior was observed both in dominant and subordinate animals. Physiological parameters indicate differences in stress levels between dominant and subordinate animals. It is concluded that aggressive behavior in group-housed male BALB/c mice is best prevented by housing the animals in small groups of three to five animals, while decreasing floor size per animal may be used as a temporary solution to decrease high levels of aggression in an existing social group.

Nucleosome repositioning during differentiation of a human myeloid leukemia cell line

Cell differentiation is associated with changes in chromatin organization and gene expression. In this study, we examine chromatin structure following differentiation of the human myeloid leukemia cell line (HL-60/S4) into granulocytes with retinoic acid (RA) or into macrophage with phorbol ester (TPA). We performed ChIP-seq of histone H3 and its modifications, analyzing changes in nucleosome occupancy, nucleosome repeat length, eu-/heterochromatin redistribution and properties of epichromatin (surface chromatin adjacent to the nuclear envelope). Nucleosome positions changed genome-wide, exhibiting a specific class of alterations involving nucleosome loss in extended (∼1kb) regions, pronounced in enhancers and promoters. Genes that lost nucleosomes at their promoters showed a tendency to be upregulated. On the other hand, nucleosome gain did not show simple effects on transcript levels. The average genome-wide nucleosome repeat length (NRL) did not change significantly with differentiation. However, we detected an approximate 10 bp NRL decrease around the haematopoietic transcription factor (TF) PU.1 and the architectural protein CTCF, suggesting an effect on NRL proximal to TF binding sites. Nucleosome occupancy changed in regions associated with active promoters in differentiated cells, compared with untreated HL-60/S4 cells. Epichromatin regions revealed an increased GC content and high nucleosome density compared with surrounding chromatin. Epichromatin showed depletion of major histone modifications and revealed enrichment with PML body-associated genes. In general, chromatin changes during HL-60/S4 differentiation appeared to be more localized to regulatory regions, compared with genome-wide changes among diverse cell types studied elsewhere

Seminal fluid compromises visual perception in honeybee queens reducing their survival during additional mating flights

Queens of social insects make all mate-choice decisions on a single day, except in honeybees whose queens can conduct mating flights for several days even when already inseminated by a number of drones. Honeybees therefore appear to have a unique, evolutionarily derived form of sexual conflict: a queen's decision to pursue risky additional mating flights is driven by later-life fitness gains from genetically more diverse worker-offspring but reduces paternity shares of the drones she already mated with. We used artificial insemination, RNA-sequencing and electroretinography to show that seminal fluid induces a decline in queen vision by perturbing the phototransduction pathway within 24-48 hr. Follow up field trials revealed that queens receiving seminal fluid flew two days earlier than sister queens inseminated with saline, and failed more often to return. These findings are consistent with seminal fluid components manipulating queen eyesight to reduce queen promiscuity across mating flights