Effects of non-steroidal growth implant and dietary zilpaterol hydrochloride on growth and carcass characteristics of feedlot lambs

The effects of steroidal growth implants alone or in combination with β-adrenergic agonist feed additives have not been studied thoroughly in mutton sheep in South African feedlot conditions. This study investigated the effects of a non-steroidal growth implant zeranol (Ralgro®), alone or in combination with zilpaterol hydrochloride (Zilmax®), on growth performance, carcass characteristics, and selected meat quality traits in 160 SA Mutton Merino ram lambs fed in a commercial feedlot. The experimental design consisted of two Ralgro treatment groups x two Zilmax treatments x two Zilmax feeding periods x 20 animals per treatment group. Lambs were randomly allocated to eight treatment groups, of which one half were implanted with Ralgro after arrival, followed by feed supplementation with Zilmax at two Zilmax feeding periods during the finisher phase, compared with negative control treatments (e.g. no Ralgro or Zilmax; Ralgro + no Zilmax; or no Ralgro + Zilmax). All lambs were fed the grower ration traditionally used by the commercial feedlot, which contained 16.89% crude protein (CP) and 2.94 Mcal/kg dry matter (DM). Zilmax was supplemented in the feed at 40 mg per animal per day and fed for 18 days or 25 days, plus three days withdrawal. Lambs that did not receive the Zilmax treatment were fed the basal diet without Zilmax feed supplementation. Ralgro significantly increased average daily gains (ADG) and cold carcass mass (CCM) of lambs. Lambs supplemented with Zilmax during the finisher phase had higher ADG, greater CCM and increased dressing percentage by ca.1.1% compared with those in the control group. Lambs fed Zilmax for 25 days had higher CCM than those fed for 18 days. The duration of the Zilmax treatment also decreased hide weight, fat thickness and shear force values (tenderness). Zilmax increased ADG and CCM in lambs, particularly if combined with Ralgro implants during the growing phase. The results from this study indicate that the combination of Ralgro implants with Zilmax feed supplementation during the finisher phase had additive effects and increased ADG and CCM of feedlot lambs.Keywords: Growth performance, meat quality, South African Mutton Merino, sheep, Ralgro®, Zilmax

Secure referee selection for fair and responsive peer-to-peer gaming

Peer-to-Peer (P2P) architectures for Massively Multiplayer Online Games (MMOG) provide better scalability than Client/Server (C/S); however, they increase the possibility of cheating. Recently proposed P2P protocols use trusted referees that simulate/validate the game to provide security equivalent to C/S. When selecting referees from untrusted peers, selecting non-colluding referees becomes critical. Further, referees should be selected such that the range and length of delays to players is minimised (maximising game fairness and responsiveness). In this paper we formally define the referee selection problem and propose two secure referee selection algorithms, SRS-1 and SRS-2, to solve it. Both algorithms ensure the probability of corrupt referees controlling a zone/region is below a predefined limit, while attempting to maximise responsiveness and fairness. The trade-off between responsiveness and fairness is adjustable for both algorithms. Simulations of three different scenarios show the effectiveness of our algorithms

First direct measurements of hydraulic jumps in an active submarine density current

For almost half a century, it has been suspected that hydraulic jumps, which consist of a sudden decrease in downstream velocity and increase in flow thickness, are an important feature of submarine density currents such as turbidity currents and debris flows. Hydraulic jumps are implicated in major seafloor processes, including changes from channel erosion to fan deposition, flow transformations from debris flow to turbidity current, and large-scale seafloor scouring. We provide the first direct evidence of hydraulic jumps in a submarine density current and show that the observed hydraulic jumps are in phase with seafloor scours. Our measurements reveal strong vertical velocities across the jumps and smaller than predicted decreases in downstream velocity. Thus, we demonstrate that hydraulic jumps need not cause instantaneous and catastrophic deposition from the flow as previously suspected. Furthermore, our unique data set highlights problems in using depth-averaged velocities to calculate densimetric Froude numbers for gravity currents

Gastric cancer and Helicobacter pylori: a combined analysis of 12 case control studies nested within prospective cohorts

BACKGROUND: The magnitude of the association between Helicobacter pylori and incidence of gastric cancer is unclear. H pylori infection and the circulating antibody response can be lost with development of cancer; thus retrospective studies are subject to bias resulting from classifi- cation of cases as H pylori negative when they were infected in the past. AIMS: To combine data from all case control studies nested within prospective cohorts to assess more reliably the relative risk of gastric cancer associated with H pylori infection.To investigate variation in relative risk by age, sex, cancer type and subsite, and interval between blood sampling and cancer diagnosis. METHODS: Studies were eligible if blood samples for H pylori serology were collected before diagnosis of gastric cancer in cases. Identified published studies and two unpublished studies were included. Individual subject data were obtained for each. Matched odds ratios (ORs) and 95% confidence intervals (95% CI) were calculated for the association between H pylori and gastric cancer. RESULTS: Twelve studies with 1228 gastric cancer cases were considered. The association with H pylori was restricted to noncardia cancers (OR 3.0; 95% CI 2.3–3.8) and was stronger when blood samples for H pylori serology were collected 10+ years before cancer diagnosis (5.9; 3.4–10.3). H pylori infection was not associated with an altered overall risk of cardia cancer (1.0; 0.7–1.4). CONCLUSIONS: These results suggest that 5.9 is the best estimate of the relative risk of non-cardia cancer associated with H pylori infection and that H pylori does not increase the risk of cardia cancer. They also support the idea that when H pylori status is assessed close to cancer diagnosis, the magnitude of the non-cardia association may be underestimated

Attenuation of doxorubicin-induced cardiotoxicity in a human in vitro cardiac model by the induction of the NRF-2 pathway

Dose-dependent cardiotoxicity is the leading adverse reaction seen in cancer patients treated with doxorubicin. Currently, dexrazoxane is the only approved drug that can partially protect against this toxicity in patients, however, its administration is restricted to those patients receiving a high cumulative dose of anthracyclines. Investigations into the mechanisms of cardiotoxicity and efforts to improve cardioprotective strategies have been hindered by the limited availability of a phenotypically relevant in vitro adult human cardiac model system. Here, we adapted a readily reproducible, functional 3D human multi-cell type cardiac system to emulate patient responses seen with doxorubicin and dexrazoxane. We show that administration of two NRF2 gene inducers namely the semi-synthetic triterpenoid Bardoxolone methyl, and the isothiocyanate sulfurophane, result in cardioprotection against doxorubicin toxicity comparable to dexrazoxane as evidenced by an increase in cell viability and a decrease in the production of reactive oxygen species. We further show a synergistic attenuation of cardiotoxicity when the NRF2 inducers and dexrazoxane are used in tandem. Taken together, our data indicate that the 3D spheroid is a suitable model to investigate drug induced cardiotoxicity and we reveal an essential role of the NRF2 pathway in cardioprotection providing a novel pharmacological mechanism and intervention route towards the alleviation of doxorubicin-induced toxicity

A critical review of adverse effects to the kidney: mechanisms, data sources and in silico tools to assist prediction

Introduction: The kidney is a major target for toxicity elicited by pharmaceuticals and environmental pollutants. Standard testing which often does not investigate underlying mechanisms has proven not to be an adequate hazard assessment approach. As such, there is an opportunity for the application of computational approaches that utilise multi-scale data based on the Adverse Outcome Pathway (AOP) paradigm, coupled with an understanding of the chemistry underpinning the molecular initiating event (MIE) to provide a deep understanding of how structural fragments of molecules relate to specific mechanisms of nephrotoxicity. The aim of this investigation was to review the current scientific landscape related to computational methods, including mechanistic data, AOPs, publicly available knowledge bases and current in silico models, for the assessment of pharmaceuticals and other chemicals with regard to their potential to elicit nephrotoxicity. A list of over 250 nephrotoxicants enriched with, where possible, mechanistic and AOP-derived understanding was compiled. Expert opinion: Whilst little mechanistic evidence has been translated into AOPs, this review identified a number of data sources of in vitro, in vivo and human data that may assist in the development of in silico models which in turn may shed light on the inter-relationships between nephrotoxicity mechanisms

Improved Measurement of the dˉ/uˉ\bar d / \bar u Asymmetry in the Nucleon Sea

Measurements of the ratio of Drell-Yan yields from an 800 \rm{GeV/c} proton beam incident on liquid hydrogen and deuterium targets are reported. Approximately 360,000 Drell-Yan muon pairs remained after all cuts on the data. From these data, the ratio of anti-down ($\bar{d}$) to anti-up ($\bar{u}$) quark distributions in the proton sea is determined over a wide range in Bjorken-$x$. These results confirm previous measurements by E866 and extend them to lower $x$. From these data, $(\bar{d}-\bar{u})$ and $\int(\bar{d}-\bar{u})dx$ are evaluated for $0.015<x<0.35$. These results are compared with parameterizations of various parton distribution functions, models and experimental results from NA51, NMC, and HERMES.Comment: 17 pages, 15 figure