Analysis of Rhodamin B on Lipstick, Blush On and Eye Shadow in Pekalongan Regency With Uv-Vis Spectrophotometer

Rhodamine B is a synthetic dye that is often misused in cosmetic products. These dyes can cause irritation to the respiratory tract, are carcinogenic and cause liver damage in high concentrations. This study aims to determine the content of rhodamine B in lipstick, blush on, and eye shadow in the Pekalongan Regency. The analysis used in this research is qualitative and quantitative analysis. Qualitative study using Thin Layer Chromatography (TLC) with silica gel F254 as stationary phase and mobile phase n-butanol, ethyl acetate, ammonia (5.5 : 2: 2.5) and then detected on UV lamps at 254 nm and 366 nm. Quantitative analysis using UV-Vis spectrophotometer at a maximum wavelength of 557 nm. The results of the qualitative analysis showed that three samples of lipstick (sample A, D and E), two samples of blush on (sample B and C), and three samples of eye shadow (sample A, B and E) contained rhodamine B. Rhodamine B levels in the sample lipstick A 0.00244%, lipstick D 0.00052%, lipstick E 0.00065%, blush on B 0.00239, blush on C 0.01091%, eye shadow A 0.00057%, eye shadow B 0.00140, and eye shadow E 0.00047%

Antioxidant activities of extracts, methanol, and n-hexane partitions of cayenne pepper (Capsicum frutescens L.)

Antioxidants are substances that can neutralize and/or stabilize free radicals. Cayenne pepper (Capsicum frutescens L.) is a natural source of antioxidants because it contains carotenoids, flavonoids, and vitamin C. This study aims to examine antioxidant activities of n-hexane, methanol, and extract of cayenne pepper (Capsicum frutescens L.) using the ferric reducing antioxidant power (FRAP) method. The extraction method was done by maceration, and the fractionation method was done by partitioning using unmixed solvents: methanol and n-hexane. The phytochemical screening test on extracts and partitions has discovered positive content of alkaloids, flavonoids, phenols, and terpenoids. Meanwhile, the TLC test used F254 silica gel as a stationary phase with chloroform: ethyl acetate as a mobile phase (6:4). The test discovered positive results containing flavonoids and phenols. Furthermore, the antioxidant activities were tested using the FRAP method with a UV-Vis spectrophotometer instrument at a maximum value of 598.05 nm and positive control of vitamin C. The results of measurement show that antioxidant activities are found in 505.67 ± 0.56 mol Fe2+/g of partition extract, 447.67 ± 16.2 mol Fe2+/g of methanol fraction, and 353± 5.29 mol Fe2+/g of n-hexane fraction

Bioaktivitas Ekstrak Kulit Buah Nanas (Ananas comosus (L.) Merr.) dalam Sabun Cuci Piring sebagai Antibakteri terhadap Bakteri Staphylococcus aureus

Kulit buah nanas (Ananas comosus (L.) Merr.) mengandung vitamin C, karotenoid, flavonoid, dan enzim bromelain yang dapat digunakan sebagai antibakteri. Enzim bromelain berfungsi sebagai antiseptik. Tujuan penelitian ini adalah untuk mengetahui bioaktivitas ekstrak kulit buah nanas pada sabun cuci piring sebagai antibakteri terhadap Staphylococcus aureus. Sabun cuci piring ekstrak kulit buah nanas diformulasikan dengan konsentrasi ekstrak yang berbeda yaitu pada 10% (FI), 20% (FII), dan 30% (FIII). Metode yang digunakan untuk uji antibakteri adalah metode difusi sumuran. Hasil pengamatan pada inkubasi selama 24 jam diperoleh rata rata zona hambat terbesar pada FIII yaitu sebesar 19,3 mm, sedangkan pada FII dan FI masing-masing diperoleh zona hambat sebesar 15 mm dan 13 mm. Kontrol positif (sabun cuci piring merk SL) menunjukkan adanya zona bening dengan rata-rata diameter zona hambat yaitu 25,3 mm. Formula yang memiliki zona hambat bakteri terbesar adalah FIII. Hasil dari penelitian ini menunjukkan adanya kemampuan kulit buah nanas untuk menghambat pertumbuhan Staphylococcus aureus sehingga dapat digunakan sebagai sabun cuci piring

Efek Ramuan Tradisional Kaliputih terhadap Kadar Glukosa Darah Puasa, SOD, HbA1c, dan Histopatologi Pankreas pada Tikus Diabetes yang Diinduksi Streptozotocin)

Prevention of DM by controlling sugar and lipid levels is the main therapy. The traditional medicine diabetes herb from Kaliputih Batur, Banjarnegara, Central Java consists of 11 kinds of medicinal plants that are empirically proven to be efficacious for diabetes mellitus. The potion is prepared by the infundation method. Three control groups (normal, positive, and negative) and three test groups with different doses (18, 36, and 54 mL/kg BW) that had previously been induced by streptozotocin were created using Sprague Dawley (SD) albino rats. Using HE staining, observations were obtained on fasting blood glucose (FBG), superoxide dismutase (SOD), HbA1c, and pancreatic organs. At doses of 36 and 54 mL/kg bw, results analysis of biochemical parameters of FBG levels revealed a substantial decline (P<0.05). At all dosages of the herb, there was a significant change in SOD activity (P<0.05), and at a dose of 18 mL/kg BW compared to negative control, there was a significant difference in HbA1c levels (P<0.05). HE staining results revealed no distinctpathological alterations in pancreatic organ at the test dose compared to negative control with necrosis in the Langerhans insula. Herbal medicine for diabetes traditional medicine from Kaliputih Batur, Banjarnegara, Central Java has decreased blood glucose.Pencegahan diabetes mellitus (DM) dengan mengontrol kadar gula dan lipid adalah terapi utama. Ramuantradisional dari Kaliputih Batur, Banjarnegara, Jawa Tengah terdiri dari 11 macam tanaman obat terbukti secara empiris berkhasiat untuk penyakit diabetes melitus. Tujuan penelitian untuk menentukan efek ramuan tradisional Kaliputih pada kadar gula darah, SOD, HbA1c, dan gambaran histopatologi pankreas pada tikus. Ramuan disiapkan dengan metode infundasi. Tikus jantan albino Sprague Dawley (SD) dibagi menjadi 3 kelompok kontrol (normal, positif dan negatif) dan 3 kelompok uji dengan 3 dosis berbeda (18, 36, dan 54 mL/kg BB) yang sebelumnya diinduksi streptozotocin. Pengamatan dilakukan terhadap kadar glukosa darah puasa (GDP), superoksida dismutase (SOD), HbA1c, dan organ pankreas secara histopatologi dengan pewarnaan HE. Hasil pemeriksaan parameter biokimia kadar GDP menunjukkan adanya penurunan yang bermakna (P<0,05) pada dosis 36 dan 54 mL/kg BB. Aktivitas SOD terdapat perbedaan yang bermakna (P<0,05) pada semua dosis ramuan, dan kadar HbA1c menunjukan perbedaan bermakna (P<0,05) pada dosis 18 mL/kg BB dibandingkan kontrol negatif. Hasil pewarnaan HE menunjukkan tidak ada perubahan patologi yang spesifik pada organ pankreas dosis uji dibandingkan dengan kontrol negatif yang mengalami nekrosis di insula Langerhans. Ramuan tradisional untuk diabetes dari Kaliputih Batur, Banjarnegara, Jawa Tengah mampu menurunkan kadar gula darah tikus diabetes

Antioxidant activities of extracts, methanol, and n-hexane partitions of cayenne pepper (Capsicum frutescens L.)

Antioxidants are substances that can neutralize and/or stabilize free radicals. Cayenne pepper (Capsicum frutescens L.) is a natural source of antioxidants because it contains carotenoids, flavonoids, and vitamin C. This study aims to examine antioxidant activities of n-hexane, methanol, and extract of cayenne pepper (Capsicum frutescens L.) using the ferric reducing antioxidant power (FRAP) method. The extraction method was done by maceration, and the fractionation method was done by partitioning using unmixed solvents: methanol and n-hexane. The phytochemical screening test on extracts and partitions has discovered positive content of alkaloids, flavonoids, phenols, and terpenoids. Meanwhile, the TLC test used F254 silica gel as a stationary phase with chloroform:ethyl acetate as a mobile phase (6:4). The test discovered positive results containing flavonoids and phenols. Furthermore, the antioxidant activities were tested using the FRAP method with a UV-Vis spectrophotometer instrument at a maximum value of 598.05 nm and positive control of vitamin C. The results of measurement show that antioxidant activities are found in 505.67 ± 0.56 mol Fe2+/g of partition extract, 447.67 ± 16.2 mol Fe2+/g of methanol fraction, and 353 ± 5.29 mol Fe2+/g of n-hexane fraction

Antioxidant activities of extracts, methanol, and n-hexane partitions of cayenne pepper (Capsicum frutescens L.)

Antioxidants are substances that can neutralize and/or stabilize free radicals. Cayenne pepper (Capsicum frutescens L.) is a natural source of antioxidants because it contains carotenoids, flavonoids, and vitamin C. This study aims to examine antioxidant activities of n-hexane, methanol, and extract of cayenne pepper (Capsicum frutescens L.) using the ferric reducing antioxidant power (FRAP) method. The extraction method was done by maceration, and the fractionation method was done by partitioning using unmixed solvents: methanol and n-hexane. The phytochemical screening test on extracts and partitions has discovered positive content of alkaloids, flavonoids, phenols, and terpenoids. Meanwhile, the TLC test used F254 silica gel as a stationary phase with chloroform:ethyl acetate as a mobile phase (6:4). The test discovered positive results containing flavonoids and phenols. Furthermore, the antioxidant activities were tested using the FRAP method with a UV-Vis spectrophotometer instrument at a maximum value of 598.05 nm and positive control of vitamin C. The results of measurement show that antioxidant activities are found in 505.67 ± 0.56 mol Fe2+/g of partition extract, 447.67 ± 16.2 mol Fe2+/g of methanol fraction, and 353 ± 5.29 mol Fe2+/g of n-hexane fraction

Hasil Review artikel" Efek Ramuan Obat Tradisional (Batra) Kaliputih Jawa Tengah, Terhadap Kolesterol, Trigliserida Dan Hdl Tikus Sprague Dawley Yang Diinduksi Streptozotosin"

Ramuan Batra asli Kaliputih adalah ramuan tradisional yang secara empiris berkhasiat untuk mengobati penyakit diabetes dengan komplikasi gagal ginjal yang terdiri dari tumbuhan obat yaitu, daun keji beling, daun kumis kucing, biji kopi arabika mentah, tunas bambu kuning, cokol tebu ireng, ganyong merah, rimpang temulawak, rimpang kunir putih, rimpang kunyit, buah belimbing wuluh dan srintil hutan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian Ramuan Batra terhadap kadar kolesterol, trigliserid dan HDL. Penelitian ini menggunakan 30 ekor tikus jantan galur Sprague Dawley yang diinduksi dengan Streptozotosin (dosis 40 mg/dl) secara intra peritonial dengan dosis tunggal. Pemberian ramuan dilakukan selama 15 hari sesuai dengan terapi yang dilakukan oleh Batra kepada pasien. Parameter yang diamati meliputi kadar kolesterol, trigiserid dan HDL menggunakan metode enzimatik–kalorimetri secara CHOD– PAP. Hasil Pengukuran kadar kolesterol dan trigliserida menunjukan penurunan dibandingkan kontrol negatif pada dosis 36 mL/kg BB dan dosis 54 mL/kg BB untuk kolesterol dan dosis 18 mL/kg BB, 36 mL/kg BB 54 mL/kg BB untuk trigliserida.Sedangkan pemeriksaan terhadap kadar HDL menunjukkan kenaikan dibandingkan kontrol negatif pada dosis 18 mL/kg BB, 36 mL/kg BB , 54 mL/kg BB. Sehingga ramuan Batra pada ketiga dosis,mampu menurunkan kadar kolesterol, trigliserida dan meningkatkan HDL