Micro-machining of diamond, sapphire and fused silica glass using a pulsed nano-second Nd:YVO4 laser

Optically transparent materials are being found in an ever-increasing array of technological applications within industries, such as automotive and communications. These industries are beginning to realize the importance of implementing surface engineering techniques to enhance the surface properties of materials. On account of the importance of surface engineering, this paper details the use of a relatively inexpensive diode-pumped solid state (DPSS) Nd:YVO4 laser to modify the surfaces of fused silica glass, diamond, and sapphire on a micrometre scale. Using threshold fluence analysis, it was identified that, for this particular laser system, the threshold fluence for diamond and sapphire ranged between 10 Jcm−2 and 35 Jcm−2 for a laser wavelength of 355 nm, dependent on the cumulative effects arising from the number of incident pulses. Through optical microscopy and scanning electron microscopy, it was found that the quality of processing resulting from the Nd:YVO4 laser varied with each of the materials. For fused silica glass, considerable cracking and deformation occurred. For sapphire, good quality features were produced, albeit with the formation of debris, indicating the requirement for post-processing to remove the observed debris. The diamond material gave rise to the best quality results, with extremely well defined micrometre features and minimal debris formation, comparative to alternative techniques such as femtosecond laser surface engineering

Sustainable futures : Christchurch and beyond

This thesis examines sustainable futures at various scales from international to local, focussing on processes occurring within Christchurch, New Zealand. In order for a sustainable future to occur, the integration of environmental, economic and social sustainability is required. However, very few examples exhibited this equal mix with some choosing to address issues in isolation but achieving positive change in the realisation of their pre-set goal. The most successful examples were those which incorporated the public into the consultation and implementation process. The Wetlands and Waterways Unit of the Christchurch City Council and the Christchurch Safer Community Council have achieved this but the Canterbury Dialogues process has not due to it being a top down process with limited public input. One result of the failure to achieve success has been the need to create or enhance an image, promoting icons or local characteristics; for instance, Christchurch-The Garden City. The result of this imagineering is that the image and reality of place become separated, particularly concerning social issues. The everyday importance of social aspects was illustrated in a survey asking Christchurch residents what were the. most important issues to them regarding a sustainable future. Large disparities were found to exist between the image and the reality of crime, education, health and air pollution issues. Closing the gap between image and reality was achieved in part by the use of indicators to simplify and illustrate each current situation. Failure to close this gap creates a polarised society where 'haves' and 'have nots' exist. When this occurs, harmonious relationships between environment, economy and society are compromised and prospects for a sustainable future appear bleak

Bystander Behavior: Understanding Undergraduate Male Involvement in Dangerous Drinking Situations

Problem drinking leaves a troubling wake on college campuses throughout the United States. This dissertation explores to what extent male college student bystanders involved in dangerous drinking situations intervene and to what extent information and training in specific bystander skills improves their likelihood to be effective, engaged bystanders. Many germane themes emerge in this study, including: a priority for undergraduate males on social activities, a lack of undergraduate male empathy for male peers, and absent sense of undergraduate male responsibility for self and others. Further, the study presents a new typology for the disengaged male, undergraduate bystander, including recommendations for campus educators in supporting this population

The role of specialist nurses in multiple sclerosis : a rapid and systematic review

Background Multiple sclerosis (MS) is a disease of the central nervous system. The cause is unknown. There are about 80–160 people with MS per 100,000 population, with twice as many women affected as men. The management of individuals with MS includes treatment of acute relapses and chronic symptoms. The care of MS patients is provided by various healthcare professionals, such as general practitioners (GPs), neurologists, physiotherapists, occupational therapists and nurses. Some MS patients have access to an MS specialist nurse, although this provision varies geographically. Objectives The aim of this report is to assess the effectiveness and relative cost-effectiveness of MS specialist nurses in improving care and outcomes for patients with MS. Methods A systematic review of the literature, involving a range of databases, was performed. Full details are described in the main report. Results Only one study was identified that tried to evaluate the benefit of MS specialist nurses. The study concluded that MS patients and their carers found the MS specialist nurse to be helpful, particularly in improving their knowledge of MS, ability to cope, mood and confidence about the future. GPs also reported finding the nurse to be helpful with their MS patients, and 40% of the GPs stated they would purchase the services of an MS specialist nurse if their practices became fundholding. However, there were considerable methodological weaknesses inherent in the study design, and it was unclear whether the results of the study could be extrapolated to other settings or to other MS patient groups. Ongoing research There are two ongoing research studies regarding MS specialist nurses. One of these studies involves the provision of MS nurses to several areas, but also has two control populations to allow evaluation of the health benefits of the nurses to MS patients and their carers. This study will help to fill the evidence gap. Costs The costs of providing MS specialist nurses consist of their yearly salary (usually NHS grade G), as well as additional costs for travelling, administration, computer and telephone use, a pension scheme, National Insurance and study leave. The MS Society of Great Britain and Northern Ireland allows a generous total yearly cost to the employer of £40,000. Conclusions The present evidence does not make it possible to comment with any certainty on the value of specialist nurses in MS. The best evidence available to the authors is specialist opinion from neurologists and nurses, and comments from patients with MS; this opinion supports the provision of MS specialist nurses. Recommendations for research Further research is needed before it will be feasible to make firm recommendations on the value of MS specialist nurses relative to other possible uses of funds

Blowing in the Wind: Using a Consumer Drone for the Collection of Humpback Whale (Megaptera novaeangliae) Blow Samples during the Arctic Polar Nights

Abstract Analysis of cetacean blow offers a unique potential for non-invasive assessments of their health. In recent years, the use of uncrewed aerial vehicles (UAVs) has revolutionized the way these samples are collected. However, the high cost and expertise associated with purpose-built waterproof UAVs, paired with the challenges of operating during difficult meteorological conditions, can be prohibitive for their standardized use worldwide. A pilot study was conducted in a Northern Norwegian fjord during winter, to assess the feasibility of using a minimally modified and affordable consumer drone to collect blow samples even during the polar nights’ challenging weather conditions. For each flight, six petri dishes were attached with velcro to a DJI Mavic 2 Pro. The flights were conducted under temperatures ranging from -1 to -18 degrees Celsius, wind speeds ranging from 9 to 31 km/h, and with the absence of the sun. During the 6-day-long boat survey, 16 blow samples were successfully collected from 11 distinct groups of humpback whales (Megaptera novaeangliae). With this study, we further validated the use of a consumer drone as a practical, affordable, and simplified tool for blow collection, functional under harsh meteorological conditions

Blowing in the Wind: Using a Consumer Drone for the Collection of Humpback Whale (Megaptera novaeangliae) Blow Samples during the Arctic Polar Nights

publishedVersio

High-efficiency endovascular gene delivery via therapeutic ultrasound

AbstractOBJECTIVESWe studied enhancement of local gene delivery to the arterial wall by using an endovascular catheter ultrasound (US).BACKGROUNDUltrasound exposure is standard for enhancement of in vitro gene delivery. We postulate that in vivo endovascular applications can be safely developed.METHODSWe used a rabbit model of arterial mechanical overdilation injury. After arterial overdilation, US catheters were introduced in bilateral rabbit femoral arteries and perfused with plasmid- or adenovirus-expressing blue fluorescent protein (BFP) or phosphate buffered saline. One side received endovascular US (2 MHz, 50 W/cm2, 16 min), and the contralateral artery did not.RESULTSRelative to controls, US exposure enhanced BFP expression measured via fluorescence 12-fold for plasmid (1,502.1 ± 927.3 vs. 18,053.9 ± 11,612 μm2, p < 0.05) and 19-fold for adenovirus (877.1 ± 577.7 vs. 17,213.15 ± 3,892 μm2, p < 0.05) while increasing cell death for the adenovirus group only (26 ± 5.78% vs. 13 ± 2.55%, p < 0.012).CONCLUSIONSEndovascular US enhanced vascular gene delivery and increased the efficiency of nonviral platforms to levels previously attained only by adenoviral strategies

X-ray structures of checkpoint kinase 2 in complex with inhibitors that target its gatekeeper-dependent hydrophobic pocket

AbstractThe serine/threonine checkpoint kinase 2 (Chk2) is an attractive molecular target for the development of small molecule inhibitors to treat cancer. Here, we report the rational design of Chk2 inhibitors that target the gatekeeper-dependent hydrophobic pocket located behind the adenine-binding region of the ATP-binding site. These compounds exhibit IC50 values in the low nanomolar range and are highly selective for Chk2 over Chk1. X-ray crystallography was used to determine the structures of the inhibitors in complex with the catalytic kinase domain of Chk2 to verify their modes of binding

Childhood overeating is associated with adverse cardiometabolic and inflammatory profiles in adolescence

Childhood eating behaviour contributes to the rise of obesity and related noncommunicable disease worldwide. However, we lack a deep understanding of biochemical alterations that can arise from aberrant eating behaviour. In this study, we prospectively associate longitudinal trajectories of childhood overeating, undereating, and fussy eating with metabolic markers at age 16 years to explore adolescent metabolic alterations related to specific eating patterns in the first 10 years of life. Data are from the Avon Longitudinal Study of Parents and Children (n = 3104). We measure 158 metabolic markers with a high-throughput (1H) NMR metabolomics platform. Increasing childhood overeating is prospectively associated with an adverse cardiometabolic profile (i.e., hyperlipidemia, hypercholesterolemia, hyperlipoproteinemia) in adolescence; whereas undereating and fussy eating are associated with lower concentrations of the amino acids glutamine and valine, suggesting a potential lack of micronutrients. Here, we show associations between early behavioural indicators of eating and metabolic markers