Chromosome Oscillations in Mitosis

Successful cell division requires a tight regulation of chromosome motion via the activity of molecular motors. Many of the key players at the origin of the forces generating the movement have been identified, but their spatial and temporal organization remains elusive. The protein complex Kinetochore on the chromosome associates with microtubules emanating from one of the spindle poles and drives the chromosome toward the pole. Chromokinesin motors on the chromosome arms also interact with microtubules, ejecting the chromosome away from the pole. In animal cells, a monooriented chromosome (associated to a single pole) periodically switches between phases of poleward and away from the pole movement[, a behavior tentatively explained so far by the existence of a complex switching mechanism within the kinetochore itself. Here we show that the interplay between the morphology of the mitotic spindle and the collective kinetics of chromokinesins can account for the highly non-linear periodic chromosome motion. Our analysis provides a natural explanation for the origin of chromosome directional instability and for the mechanism by which chromosomes feel their position in space.Comment: http://hogarth.pct.espci.fr/~pierre

Roles for Gcn5p and Ada2p in transcription and nucleotide excision repair at the Saccharomyces cerevisiae MET16 gene

Chromatin structure, transcription and repair of cyclobutane pyrimidine dimers at the MET16 gene of wild type, gcn5Δ and ada2Δ Saccharomyces cerevisiae cells were studied under repressing or derepressing conditions. These two components of the SAGA/ADA chromatin remodelling complexes are expendable for the basal transcription of MET16 but are mandatory for its full transcription induction. Despite their influence on transcription neither protein induces major changes in MET16 chromatin structure, but some minor ones occur. Repair at the coding region of the transcribed strand is faster than repair at non-transcribed regions in all strains and either growth condition. Moreover, the more MET16 is transcribed the faster the repair. The data show that by changing the transcription extent the rate of repair at each DNA strand is altered in a different way, confirming that repair at this locus is strongly modulated by its chromatin structure and transcription level. Deletion of GCN5 or ADA2 reduces repair at MET16. The results are discussed in light of the current understanding of Gcn5p and Ada2p functions, and they are the first to report a role for Ada2p in the nucleotide excision repair of the regulatory and transcribed regions of a gene

Potential applications of digital, visible, and infrared data from geostationary environmental satellites

An hourly, digital data base from the Visible/Infrared Spin-Scan Radiometer (VISSR) instrument on the GOES-1 and SMS-2 geostationary satellites is described. Several examples of developmental applications of these quantitative digital data are presented. These include a review of recent attempts to develop products that are of use to meteorologists who provide services to aviation, agriculture, forestry, hydrology, oceanography, and climatology. The sample products include high resolution thermal gradients of land and ocean surfaces, thermal change analyses, fruit frost/freeze application, cloud-top altitude analysis, analysis of hurricane characteristics, and analyses of solar insolation

Infrared High-Resolution Spectroscopy of Post-AGB Circumstellar Disks. I. HR 4049 - The Winnowing Flow Observed?

High-resolution infrared spectroscopy in the 2.3-4.6 micron region is reported for the peculiar A supergiant, single-lined spectroscopic binary HR 4049. Lines from the CO fundamental and first overtone, OH fundamental, and several H2O vibration-rotation transitions have been observed in the near-infrared spectrum. The spectrum of HR 4049 appears principally in emission through the 3 and 4.6 micron region and in absorption in the 2 micron region. The 4.6 micron spectrum shows a rich 'forest' of emission lines. All the spectral lines observed in the 2.3-4.6 micron spectrum are shown to be circumbinary in origin. The presence of OH and H2O lines confirm the oxygen-rich nature of the circumbinary gas which is in contrast to the previously detected carbon-rich material. The emission and absorption line profiles show that the circumbinary gas is located in a thin, rotating layer near the dust disk. The properties of the dust and gas circumbinary disk and the spectroscopic orbit yield masses for the individual stars, M_AI~0.58 Msolar and M_MV~0.34 Msolar. Gas in the disk also has an outward flow with a velocity of $\gtrsim$ 1 km/s. The severe depletion of refractory elements but near-solar abundances of volatile elements observed in HR 4049 results from abundance winnowing. The separation of the volatiles from the grains in the disk and the subsequent accretion by the star are discussed. Contrary to prior reports, the HR 4049 carbon and oxygen isotopic abundances are typical AGB values: 12C/13C=6^{+9}_{-4} and 16O/17O>200.Comment: 42 pages, 14 figures, Accepted by Ap

Ultraviolet HST Observations of the Jet in M87

We present new ultraviolet photometry of the jet in M87 obtained from HST WFPC2 imaging. We combine these ultraviolet data with previously published photometry for the knots of the jet in radio, optical, and X-ray, and fit three theoretical synchrotron models to the full data set. The synchrotron models consistently overpredict the flux in the ultraviolet when fit over the entire dataset. We show that if the fit is restricted to the radio through ultraviolet data, the synchrotron models can provide a good match to the data. The break frequencies of these fits are much lower than previous estimates. The implied synchrotron lifetimes for the bulk of the emitting population are longer than earlier work, but still much shorter than the estimated kinematic lifetimes of the knots. The observed X-ray flux cannot be successfully explained by the simple synchrotron models that fit the ultraviolet and optical fluxes. We discuss the possible implications of these results for the physical properties of the M87 jet. We also observe increased flux for the HST-1 knot that is consistent with previous results for flaring. This observation fills in a significant gap in the time coverage early in the history of the flare, and therefore sets constraints on the initial brightening of the flare.Comment: 14 pages, 2 figures, Accepted for publication in ApJ, changed lightcurve and caption in Figure

A Novel Taxonomy of Opportunities and Risks in Massively Multiplayer Online Role Playing Games

The explosion and rapid embrace of Massively Multiplayer Online Role Playing Games (MMORPG) has provided players with unique, fully immersive three dimensional environments in which they can express themselves in a myriad of ways. Players can develop unique skill sets, share knowledge, explore and experiment with various identities and network with like-minded individuals. Evidence suggests however, that these opportunities are accompanied with a number of risks including addiction, desensitisation and threats to the privacy of personal information. This paper presents a novel Taxonomy of opportunities and risks in the specific context of Massively Multiplayer Online Role Playing Games

The UARS microwave limb sounder version 5 data set: Theory, characterization, and validation

Nitric acid (HNO3) is a major player in processes controlling the springtime depletion of polar ozone. It is the main constituent of the Polar Stratospheric Clouds (PSCs) and a primary reservoir for reactive nitrogen. Potential variations in the stratospheric circulation and temperature may alter the extent and duration of PSCs activity, influencing the future ozone levels significantly. Monitoring HNO3 and its long-term variability, especially in polar region, is then crucial for better understanding issues related to ozone decline and expected recovery. In this study we present an intercomparison between ground based HNO3 measurements, carried out by means of the Ground-Based Millimeter-wave Spectrometer (GBMS), and two satellite data sets produced by the two NASA/JPL Microwave Limb Sounder (MLS) experiments. In particular, we compare UARS MLS measurements (1991-1999) with those carried out by the GBMS at South Pole, Antarctica (90°S), Fall of 1993 and 1995. A similar intercomparison is made between Aura MLS HNO3 observations (2004 - to date) and GBMS measurements obtained during the period February 2004 - March 2007, at the mid-latitudes/high altitudes station of Testa Grigia (45.9° N, 7.7° E, elev. 3500 m), and during polar winters 2008/09 and 2009/2010 at Thule Air Base (76.5°N 68.8°W), Greenland. We assess systematic differences between GBMS and both UARS and Aura HNO3 data sets at seven potential temperature levels (θ) spanning the range 465 – 960 K. The UARS data set advected to the South Pole shows a low bias, within 20% for all θ levels but the 960 K, with respect to GBMS measurements. A very good agreement, within 5%, is obtained between Aura and GBMS observations at Testa Grigia, while larger differences, possibly due to latitude dependent effects, are observed over Thule. These differences are under further investigations but a preliminary comparison over Thule among MLS v3, GBMS, and ACE-FTS measurements suggests that GBMS measurements carried out during winter 2009 might not be reliable. These comparisons have been performed in the framework of the NASA JPL GOZCARDS project, which is aimed at developing a long-term, global data record of the relevant stratospheric constituents in the context of ozone decline. GBMS has been selected in GOZCARDS since its HNO3 dataset, although sampling different latitudes in different years, is the only one spanning a sufficiently long time interval for cross-calibrating HNO3 measurements by the UARS and Aura MLS experiments

SCUBA sub-millimeter observations of gamma-ray bursters. I. GRB 970508, 971214, 980326, 980329, 980519, 980703

We discuss the first results of our ongoing program of Target of Opportunity observations of gamma-ray bursts (GRBs) using the SCUBA instrument on the James Clerk Maxwell Telescope. We present the results for GRB 970508, 971214, 980326, 980329, 980519, and 980703. Our most important result to date is the detection of a fading counterpart to GRB 980329 at 850 microns. Although it proved to be difficult to find the infrared counterpart to this burst, the sub-millimeter flux was relatively bright. This indicates that intrinsically the brightness of this counterpart was very similar to GRB 970508. The radio through sub-millimeter spectrum of GRB 980329 is well fit by a power law with index alpha = +0.9. However, we cannot exclude a nu^(1/3) power law attenuated by synchrotron self-absorption. An alpha = +1 VLA-SCUBA power law spectrum is definitely ruled out for GRB 980703, and possibly also for GRB 980519. We cannot rule out that part of the sub-millimeter flux from GRB 980329 comes from a dusty star-forming galaxy at high redshift, such as the ones recently discovered by SCUBA. Any quiescent dust contribution will be much larger at sub-millimeter than at radio wavelengths. Both a high redshift and large dust extinction would help explain the reddening of the counterpart to GRB 980329, and a redshift of z = 5 has been suggested. The large intensity of this burst might then indicate that beaming is important.Comment: 6 pages, 3 figures, submitted to Astronomy and Astrophysic