5,680 research outputs found
Emergent behaviour in a chlorophenol-mineralising three-tiered microbial `food web'
Anaerobic digestion enables the water industry to treat wastewater as a
resource for generating energy and recovering valuable by-products. The
complexity of the anaerobic digestion process has motivated the development of
complex models. However, this complexity makes it intractable to pin-point
stability and emergent behaviour. Here, the widely used Anaerobic Digestion
Model No. 1 (ADM1) has been reduced to its very backbone, a syntrophic
two-tiered microbial food chain and a slightly more complex three-tiered
microbial food web, with their stability analysed as function of the inflowing
substrate concentration and dilution rate. Parameterised for phenol and
chlorophenol degradation, steady-states were always stable and non-oscillatory.
Low input concentrations of chlorophenol were sufficient to maintain
chlorophenol- and phenol-degrading populations but resulted in poor conversion
and a hydrogen flux that was too low to sustain hydrogenotrophic methanogens.
The addition of hydrogen and phenol boosted the populations of all three
organisms, resulting in the counterintuitive phenomena that (i) the phenol
degraders were stimulated by adding hydrogen, even though hydrogen inhibits
phenol degradation, and (ii) the dechlorinators indirectly benefitted from
measures that stimulated their hydrogenotrophic competitors; both phenomena
hint at emergent behaviour.Comment: 19 pages, 8 figure
Ground Anchors Stabilize Highway Bridge Abutments
Calgary\u27s Glenmore Causeway, constructed in 1962 across the Elbow River, consists of an earthfill embankment with a waterway spanned by a 43 m long bridge, the abutments of which are supported on spread footings founded in the embankment fill. Between the 25 m deep sand and gravel embankment fill and the sub-horizontal bedrock surface is a compressible clay layer up to 5 m thick. To improve abutment stability during peak river flows, 126 post-tensioned Dywidag ground anchors up to 60 m long were installed through the concrete slabs armouring the abutment slopes. Twenty-two anchors were terminated in the embankment fill and the rest were grouted into bedrock. After a series of lift-off tests and anchor retensioning to compensate for ground consolidation, a procedure for predicting the rate of anchor load relaxation was developed. It was concluded the anchors are performing satisfactorily although periodic re-tensioning will be required
Effects of sex chromosome dosage on corpus callosum morphology in supernumerary sex chromosome aneuploidies.
BackgroundSupernumerary sex chromosome aneuploidies (sSCA) are characterized by the presence of one or more additional sex chromosomes in an individual's karyotype; they affect around 1 in 400 individuals. Although there is high variability, each sSCA subtype has a characteristic set of cognitive and physical phenotypes. Here, we investigated the differences in the morphometry of the human corpus callosum (CC) between sex-matched controls 46,XY (N =99), 46,XX (N =93), and six unique sSCA karyotypes: 47,XYY (N =29), 47,XXY (N =58), 48,XXYY (N =20), 47,XXX (N =30), 48,XXXY (N =5), and 49,XXXXY (N =6).MethodsWe investigated CC morphometry using local and global area, local curvature of the CC boundary, and between-landmark distance analysis (BLDA). We hypothesized that CC morphometry would vary differentially along a proposed spectrum of Y:X chromosome ratio with supernumerary Y karyotypes having the largest CC areas and supernumerary X karyotypes having significantly smaller CC areas. To investigate this, we defined an sSCA spectrum based on a descending Y:X karyotype ratio: 47,XYY, 46,XY, 48,XXYY, 47,XXY, 48,XXXY, 49,XXXXY, 46,XX, 47,XXX. We similarly explored the effects of both X and Y chromosome numbers within sex. Results of shape-based metrics were analyzed using permutation tests consisting of 5,000 iterations.ResultsSeveral subregional areas, local curvature, and BLDs differed between groups. Moderate associations were found between area and curvature in relation to the spectrum and X and Y chromosome counts. BLD was strongly associated with X chromosome count in both male and female groups.ConclusionsOur results suggest that X- and Y-linked genes have differential effects on CC morphometry. To our knowledge, this is the first study to compare CC morphometry across these extremely rare groups
Of?p stars: a class of slowly rotating magnetic massive stars
Only 5 Of?p stars have been identified in the Galaxy. Of these, 3 have been
studied in detail, and within the past 5 years magnetic fields have been
detected in each of them. The observed magnetic and spectral characteristics
are indicative of organised magnetic fields, likely of fossil origin, confining
their supersonic stellar winds into dense, structured magnetospheres. The
systematic detection of magnetic fields in these stars strongly suggests that
the Of?p stars represent a general class of magnetic O-type stars.Comment: Proceedings of IAUS 272: Active OB star
Discovery of a strong magnetic field in the rapidly rotating B2Vn star HR 7355
We report the detection of a strong, organized magnetic field in the
helium-variable early B-type star HR 7355 using spectropolarimetric data
obtained with ESPaDOnS on the 3.6-m Canada-France-Hawaii Telescope within the
context of the Magnetism in Massive Stars (MiMeS) Large Program. HR 7355 is
both the most rapidly rotating known main-sequence magnetic star and the most
rapidly rotating helium-strong star, with = 300 15 km s
and a rotational period of 0.5214404 0.0000006 days. We have modeled our
eight longitudinal magnetic field measurements assuming an oblique dipole
magnetic field. Constraining the inclination of the rotation axis to be between
and , we find the magnetic obliquity angle to be
between and , and the polar strength of the magnetic
field at the stellar surface to be between 13-17 kG. The photometric light
curve constructed from HIPPARCOS archival data and new CTIO measurements shows
two minima separated by 0.5 in rotational phase and occurring 0.25 cycles
before/after the magnetic extrema. This photometric behavior coupled with
previously-reported variable emission of the H line (which we confirm)
strongly supports the proposal that HR 7355 harbors a structured magnetosphere
similar to that in the prototypical helium-strong star, Ori E.Comment: 6 pages, 3 figures. Accepted for publication in MNRAS Letter
Elasticity Theory Connection Rules for Epitaxial Interfaces
Elasticity theory provides an accurate description of the long-wavelength
vibrational dynamics of homogeneous crystalline solids, and with supplemental
boundary conditions on the displacement field can also be applied to abrupt
heterojunctions and interfaces. The conventional interface boundary conditions,
or connection rules, require that the displacement field and its associated
stress field be continuous through the interface. We argue, however, that these
boundary conditions are generally incorrect for epitaxial interfaces, and we
give the general procedure for deriving the correct conditions, which depend
essentially on the detailed microscopic structure of the interface. As a simple
application of our theory we analyze in detail a one-dimensional model of an
inhomogeneous crystal, a chain of harmonic oscillators with an abrupt change in
mass and spring stiffness parameters. Our results have implications for phonon
dynamics in nanostructures such as superlattices and nanoparticles, as well as
for the thermal boundary resistance at epitaxial interfaces.Comment: 7 pages, Revte
Ranking ligand affinity for the DNA minor groove by experiment and simulation
The structural and thermodynamic basis for the strength and selectivity of the interactions of minor-groove binders (MGBs) with DNA is not fully understood. In 2003 we reported the first example of a thiazole containing MGB that bound in a phase shifted pattern that spanned 6 base-pairs rather than the usual 4 (for tricyclic distamycin-like compounds). Since then, using DNA footprinting, nuclear magnetic resonance spectroscopy, isothermal titration calorimetry and molecular dynamics, we have established that the flanking bases around the central 4 being read by the ligand have subtle effects on recognition. We have investigated the effect of these flanking sequences on binding and the reasons for the differences and established a computational method to rank ligand affinity against varying DNA sequences
Studying the photometric and spectroscopic variability of the magnetic hot supergiant Orionis Aa
Massive stars play a significant role in the chemical and dynamical evolution
of galaxies. However, much of their variability, particularly during their
evolved supergiant stage, is poorly understood. To understand the variability
of evolved massive stars in more detail, we present a study of the O9.2Ib
supergiant Ori Aa, the only currently confirmed supergiant to host a
magnetic field. We have obtained two-color space-based BRIght Target Explorer
photometry (BRITE) for Ori Aa during two observing campaigns, as well
as simultaneous ground-based, high-resolution optical CHIRON spectroscopy. We
perform a detailed frequency analysis to detect and characterize the star's
periodic variability. We detect two significant, independent frequencies, their
higher harmonics, and combination frequencies: the stellar rotation period
d, most likely related to the presence of the
stable magnetic poles, and a variation with a period of d
attributed to circumstellar environment, also detected in the H and
several He I lines, yet absent in the purely photospheric lines. We confirm the
variability with /4, likely caused by surface
inhomogeneities, being the possible photospheric drivers of the discrete
absorption components. No stellar pulsations were detected in the data. The
level of circumstellar activity clearly differs between the two BRITE observing
campaigns. We demonstrate that Ori Aa is a highly variable star with
both periodic and non-periodic variations, as well as episodic events. The
rotation period we determined agrees well with the spectropolarimetric value
from the literature. The changing activity level observed with BRITE could
explain why the rotational modulation of the magnetic measurements was not
clearly detected at all epochs.Comment: 20 pages, 5 tables, 12 figures, accepted for publication in A&
Stimulation of shrimp (Penaeus monodon) hemocytes by lipopolysaccharide-like molecules derived from Novacq™
Immune stimulation through feed additives is a promising strategy that can help to combat disease in shrimp farming and reduce the use of antibiotics and other chemotherapeutics. The present study investigated the in vitro immunostimulatory effects of lipopolysaccharide (LPS)-like molecules isolated from the microbial based feed additive Novacq™ (N-LPS). The presence of LPS-like molecules was confirmed and quantified Novacq™ using a HEK-TLR4 reporter cell line. Primary hemocytes isolated from adult Penaeus monodon were used to measure the immunostimulatory of N-LPS compared with the control group that were treated with E. coli derived LPS (E-LPS). The N-LPS stimulated a rapid and significant induction of the phenoloxidase (PO) response in the hemocytes. The PO response increased with exposure time and LPS concentration and was significantly higher compared with an E. coli LPS (E-LPS) control. In addition, using gene expression data, we quantified the transcriptome response of the hemocytes at 15, 30 and 60 mins post stimulation. Compared with the controls, the N-LPS treated hemocytes had a significant up-regulation of genes involved in the immune system modulation and control at all time-points. Most noteworthy was the significant induction of transcripts that function as serine protease inhibitors (namely SERPINs), that regulate the overexpression of the PO system. Transcription factors from the Notch family which directly regulate the expression of many immune genes were also induced within the hemocytes. Additionally, we also saw a strong up-regulation of crustacean hyperglycemic hormone (CHH) transcripts, an important neuropeptide involved in immune function. Overall, the transcriptome profile of the hemocytes suggests that the LPS component of Novacq™ is highly immunostimulatory and generates a strong PO response in vitro. The subsequent transcriptional response appears to be directed towards preventing further activation of the PO system most likely in an attempt to limit cytoxicity to the host. Our study highlights the immunostimulatory ability of Novacq™ and provides further evidence of the positive health benefits this microbial based feed additive can have in shrimp.</p
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