74 research outputs found
Effective charge of the [pi]h11/2 orbital and the electric field gradient of Hg from the yrast structure of 206Hg
The Îł-ray decay of excited states of the two-proton hole nucleus, 206Hg, has been identified using Gammasphere and 208Pb+238U collisions. The yrast states found include a T1/2 = 92(8)ns 10+ isomer located above the known 5- isomer. The B(E2;10+â8+) strength is used to derive the quadrupole polarization charge induced by the h11/2 proton hole. Also, the implied quadrupole moment has been used to provide an absolute scale for the electric field gradient of Hg in Hg metal
Escherichia coli MazF Leads to the Simultaneous Selective Synthesis of Both âDeath Proteinsâ and âSurvival Proteinsâ
The Escherichia coli mazEF module is one of the most thoroughly studied toxinâantitoxin systems. mazF encodes a stable toxin, MazF, and mazE encodes a labile antitoxin, MazE, which prevents the lethal effect of MazF. MazF is an endoribonuclease that leads to the inhibition of protein synthesis by cleaving mRNAs at ACA sequences. Here, using 2D-gels, we show that in E. coli, although MazF induction leads to the inhibition of the synthesis of most proteins, the synthesis of an exclusive group of proteins, mostly smaller than about 20 kDa, is still permitted. We identified some of those small proteins by mass spectrometry. By deleting the genes encoding those proteins from the E. coli chromosome, we showed that they were required for the death of most of the cellular population. Under the same experimental conditions, which induce mazEF-mediated cell death, other such proteins were found to be required for the survival of a small sub-population of cells. Thus, MazF appears to be a regulator that induces downstream pathways leading to death of most of the population and the continued survival of a small sub-population, which will likely become the nucleus of a new population when growth conditions become less stressful
Immunomodulation with dendritic cells and donor lymphocyte infusion converge to induce graft vs neuroblastoma reactions without GVHD after allogeneic bone marrow transplantation
A Pilot Study of IL-2Rα Blockade during Lymphopenia Depletes Regulatory T-cells and Correlates with Enhanced Immunity in Patients with Glioblastoma
Preclinical studies in mice have demonstrated that the prophylactic depletion of immunosuppressive regulatory T-cells (T(Regs)) through targeting the high affinity interleukin-2 (IL-2) receptor (IL-2Rα/CD25) can enhance anti-tumor immunotherapy. However, therapeutic approaches are complicated by the inadvertent inhibition of IL-2Rα expressing anti-tumor effector T-cells.To determine if changes in the cytokine milieu during lymphopenia may engender differential signaling requirements that would enable unarmed anti-IL-2Rα monoclonal antibody (MAbs) to selectively deplete T(Regs) while permitting vaccine-stimulated immune responses.A randomized placebo-controlled pilot study was undertaken to examine the ability of the anti-IL-2Rα MAb daclizumab, given at the time of epidermal growth factor receptor variant III (EGFRvIII) targeted peptide vaccination, to safely and selectively deplete T(Regs) in patients with glioblastoma (GBM) treated with lymphodepleting temozolomide (TMZ).Daclizumab treatment (n = 3) was well-tolerated with no symptoms of autoimmune toxicity and resulted in a significant reduction in the frequency of circulating CD4+Foxp3+ TRegs in comparison to saline controls (n = 3)( p = 0.0464). A significant (p<0.0001) inverse correlation between the frequency of TRegs and the level of EGFRvIII specific humoral responses suggests the depletion of TRegs may be linked to increased vaccine-stimulated humoral immunity. These data suggest this approach deserves further study.ClinicalTrials.gov NCT00626015
Extended Thromboprophylaxis with Betrixaban in Acutely Ill Medical Patients
Background
Patients with acute medical illnesses are at prolonged risk for venous thrombosis. However, the appropriate duration of thromboprophylaxis remains unknown.
Methods
Patients who were hospitalized for acute medical illnesses were randomly assigned to receive subcutaneous enoxaparin (at a dose of 40 mg once daily) for 10±4 days plus oral betrixaban placebo for 35 to 42 days or subcutaneous enoxaparin placebo for 10±4 days plus oral betrixaban (at a dose of 80 mg once daily) for 35 to 42 days. We performed sequential analyses in three prespecified, progressively inclusive cohorts: patients with an elevated d-dimer level (cohort 1), patients with an elevated d-dimer level or an age of at least 75 years (cohort 2), and all the enrolled patients (overall population cohort). The statistical analysis plan specified that if the between-group difference in any analysis in this sequence was not significant, the other analyses would be considered exploratory. The primary efficacy outcome was a composite of asymptomatic proximal deep-vein thrombosis and symptomatic venous thromboembolism. The principal safety outcome was major bleeding.
Results
A total of 7513 patients underwent randomization. In cohort 1, the primary efficacy outcome occurred in 6.9% of patients receiving betrixaban and 8.5% receiving enoxaparin (relative risk in the betrixaban group, 0.81; 95% confidence interval [CI], 0.65 to 1.00; P=0.054). The rates were 5.6% and 7.1%, respectively (relative risk, 0.80; 95% CI, 0.66 to 0.98; P=0.03) in cohort 2 and 5.3% and 7.0% (relative risk, 0.76; 95% CI, 0.63 to 0.92; P=0.006) in the overall population. (The last two analyses were considered to be exploratory owing to the result in cohort 1.) In the overall population, major bleeding occurred in 0.7% of the betrixaban group and 0.6% of the enoxaparin group (relative risk, 1.19; 95% CI, 0.67 to 2.12; P=0.55).
Conclusions
Among acutely ill medical patients with an elevated d-dimer level, there was no significant difference between extended-duration betrixaban and a standard regimen of enoxaparin in the prespecified primary efficacy outcome. However, prespecified exploratory analyses provided evidence suggesting a benefit for betrixaban in the two larger cohorts. (Funded by Portola Pharmaceuticals; APEX ClinicalTrials.gov number, NCT01583218. opens in new tab.
NK-cell-dependent killing of colon carcinoma cells is mediated by natural cytotoxicity receptors (NCRs) and stimulated by parvovirus infection of target cells
Nicomache (Loxochona) lokii sp. nov. (Annelida: Polychaeta: Maldanidae) from the Lokiâs Castle vent field: an important structure builder in an Arctic vent system
Activation of the human immune system by chemotherapeutic or targeted agents combined with the oncolytic parvovirus H-1
AnĂĄlise do lĂquido cĂ©rebro-espinhal de trĂȘs doenças do sistema nervoso central de cĂŁes
RESUMO: Foi realizado um estudo retrospectivo do lĂquido cĂ©rebro-espinhal de cĂŁes (LCE), atendidos pelo Serviço de Neurologia do Hospital VeterinĂĄrio da Instituição, de 2004 a 2015, com o objetivo de analisar os resultados de cĂŁes com sinais neurolĂłgicos, comparar as alteraçÔes encontradas em dois locais de colheita no mesmo paciente e verificar se esse exame auxiliou o clĂnico em reforçar a suspeita clĂnica das principais doenças do sistema nervoso central. A pleocitose linfocĂtica esteve presente em 78,3% (29/37) das amostras de cĂŁes com cinomose e em 23,2% (10/43) de cĂŁes com DDIV. Houve dissociação albuminocitolĂłgica (DAC) em 73% (19/26) das amostras de cĂŁes com tumores IC e em 64,3% (9/14) de cĂŁes com tumores envolvendo a ME. Em cĂŁes com DDIV, houve significĂąncia estatĂstica (p<0,05) entre o grau de disfunção neurolĂłgica e o total de cĂ©lulas nucleadas (TCN) e total de proteĂnas (TP). Em 29 cĂŁes, houve a colheita do LCE da cisterna magna e da cisterna lombar e em 12 (41,4%) os resultados foram diferentes entre as duas amostras colhidas do mesmo cĂŁo, onde dois (6,9%) apresentaram alteração na amostra colhida cranial Ă lesĂŁo. Pode-se concluir que a pleocitose linfocĂtica foi a principal alteração encontrada no LCE de cĂŁes com cinomose e DDIV e DAC nas neoplasias, IC e ME, cĂŁes acometidos pela DDIV apresentaram sinais neurolĂłgicos mais severos conforme o TCN e o TP aumentaram e o LCE sofreu alteração, mesmo colhido cranial ao local da lesĂŁo e auxiliou o clĂnico em reforçar a suspeita clĂnica, mas nĂŁo confirmou, as principais doenças neurolĂłgicas em cĂŁes
- âŠ