Polarisation correlation in the two-photon decay of metastable atomic deuterium and a test of Bell's inequality

A 1-keV metastable atomic deuterium [D(2S)] beam of density about 104 atoms cm-3 has been produced by charge-exchange, in caesium vapour, of deuterons extracted from a radio-frequency ion source. The linear and circular polarisation correlation of the two photons emitted spontaneously by metastable atomic deuterium in a true second order radiative process have been measured for the first time. While the circular polarisation results confirm the conservation of angular momentum along the common axis of detection, the correlation in linear polarisation has been applied in a test of Bell’s Inequality which allows a quantitative distinction to be made between the predictions of Quantum Mechanics and local realistic ("hidden variable") theories. The results are in agreement with Quantum Mechanics and violate Bell’s Inequality by almost two standard deviations

Anaesthetists’ knowledge of surgical antibiotic prophylaxis: a prospective descriptive study

Background: Surgical site infection (SSI) is the second most common hospital-acquired infection and results in increased morbidity and mortality and a longer hospital stay. Surgical antibiotic prophylaxis (SAP) is one component of broader strategies to reduce rates of SSI. Adherence to SAP guidelines is largely sub-optimal globally, with knowledge of appropriate SAP being an important factor that affects this. The study’s objective was to describe awareness amongst anaesthetists at university-affiliated hospitals of available SAP guidelines and to describe their knowledge on the subject. Comparisons between senior and junior anaesthetists were to be made.Methodology: A prospective descriptive study design using a self-administered questionnaire was employed. The study population was the anaesthetists in a university-affiliated Department of Anaesthesiology in Johannesburg, South Africa.Results: The analysis included 135 completed questionnaires from the department’s anaesthetists. A total of 15.6% of participants followed a specific guideline in their practice, 28% for senior anaesthetists vs. 4.2% for junior anaesthetists. The overall mean score for knowledge was 56.2%, 59.3% for senior anaesthetists vs. 53.6% for junior anaesthetists, which was statistically significant (p-value < 0.001). Overall knowledge was found to be poor and, specifically, knowledge regarding indication for prophylaxis, antibiotic re-dosing interval and duration of prophylaxis was poor.Conclusion: The anaesthetists had poor knowledge regarding SAP. While the difference in knowledge between senior and junior anaesthetists was statistically significant, is it likely that this difference would not be substantial enough to have a clinical impact. The authors recommend interventions to improve the knowledge of the anaesthetists regarding SAP as well as the development of local SAP guidelines.Keywords: anaesthetist, antibiotics, knowledge, perioperative, surgical prophylaxis, surgical site infectio

Ultrafast laser parallel microdrilling using multiple annular beams generated by a spatial light modulator

Ultrafast laser parallel microdrilling using diffractive multiple annular beam patterns is demonstrated in this paper. The annular beam was generated by diffractive axicon computer generated holograms (CGHs) using a spatial light modulator. The diameter of the annular beam can be easily adjusted by varying the radius of the smallest ring in the axicon. Multiple annular beams with arbitrary arrangement and multiple annular beam arrays were generated by superimposing an axicon CGH onto a grating and lenses algorithm calculated multi-beam CGH and a binary Dammann grating CGH, respectively. Microholes were drilled through a 0.03 mm thick stainless steel foil using the multiple annular beams. By avoiding huge laser output attenuation and mechanical annular scanning, the processing is ~200 times faster than the normal single beam processing

Scenario changes in the climatology of winter midlatitude cyclone activity over eastern North America and the Northwest Atlantic

The present study explores how midlatitude winter cyclone activity can be modified under warming-induced conditions due to enhanced greenhouse gas concentrations. We performed simulations with the Canadian Regional Climate Model (CRCM version 3.5) implemented on a domain that covers the Northwest Atlantic and eastern North America. These simulations are driven by control conditions (1975–1994) and high-CO2 scenario conditions (2040–2059) suggested by the Canadian Climate Centre model, CGCM2 (Second Generation Coupled Global Climate Model), following the IPCC IS92a scenario. Comparisons between model simulations for the control period (1975–1994) and North America Regional analysis (NARR) suggest that both CGCM2 and CRCM reliably reproduce the overall NARR patterns of sea level pressure, tropospheric baroclinicity and Atlantic storm tracks. However, compared to CGCM2 results, CRCM offers an improvement in simulations of the most intense cyclones. Although both models underestimate the track density of intense cyclones, the CGCM2 underestimates are larger than those of CRCM. Under the high-CO2 climate change scenario, the CRCM and CGCM2 model simulations show similar changes in sea level pressure, surface temperature, and total track density of midlatitude winter cyclones. Although we can see the northwest shift of the dominant Atlantic storm track, it is not statistically significant. Moreover, simulations from both models show a decrease in the total cyclone track density along the Canadian east coast; the decrease is more robust in CRCM simulations than in CGCM2 results. For intense cyclones, CRCM simulations show a slight decrease in the track density, while no such change is found in CGCM2 simulations

Correlating liposomal adjuvant characteristics to in-vivo cell-mediated immunity using a novel Mycobacterium tuberculosis fusion protein : a multivariate analysis study

OBJECTIVE: In this study, we have used a chemometrics-based method to correlate key liposomal adjuvant attributes with in-vivo immune responses based on multivariate analysis. METHODS: The liposomal adjuvant composed of the cationic lipid dimethyldioctadecylammonium bromide (DDA) and trehalose 6,6-dibehenate (TDB) was modified with 1,2-distearoyl-sn-glycero-3-phosphocholine at a range of mol% ratios, and the main liposomal characteristics (liposome size and zeta potential) was measured along with their immunological performance as an adjuvant for the novel, postexposure fusion tuberculosis vaccine, Ag85B-ESAT-6-Rv2660c (H56 vaccine). Partial least square regression analysis was applied to correlate and cluster liposomal adjuvants particle characteristics with in-vivo derived immunological performances (IgG, IgG1, IgG2b, spleen proliferation, IL-2, IL-5, IL-6, IL-10, IFN-γ). KEY FINDINGS: While a range of factors varied in the formulations, decreasing the 1,2-distearoyl-sn-glycero-3-phosphocholine content (and subsequent zeta potential) together built the strongest variables in the model. Enhanced DDA and TDB content (and subsequent zeta potential) stimulated a response skewed towards a cell mediated immunity, with the model identifying correlations with IFN-γ, IL-2 and IL-6. CONCLUSION: This study demonstrates the application of chemometrics-based correlations and clustering, which can inform liposomal adjuvant design

Polarization correlations in the two--photon decay of hydrogen--like ions

Polarization properties of the photons emitted in the two-photon decay of hydrogen-like ions are studied within the framework of the density matrix and second-order perturbation theory. In particular, we derive the polarization correlation function that gives the probability of the (two-photon) coincidence measurement performed by polarization-sensitive detectors. Detailed calculations of this function are performed for the $2s_{1/2} \to 1s_{1/2}$ transition in neutral hydrogen as well as Xe$^{53+}$ and U$^{91+}$ ions. The obtained results allow us to understand the influence of relativistic and non-dipole effects on the polarization correlations in the bound-bound two-photon transitions in heavy ions

Th1 immune responses can be modulated by varying dimethyldioctadecylammonium and distearoyl-sn-glycero-3-phosphocholine content in liposomal adjuvants

OBJECTIVES: Cationic liposomes of dimethyldioctadecylammonium bromide (DDA) combined with trehalose 6,6'-dibehenate (TDB) elicit strong cell-mediated and antibody immune responses; DDA facilitates antigen adsorption and presentation while TDB potentiates the immune response. To further investigate the role of DDA, DDA was replaced with the neutral lipid of distearoyl-sn-glycero-3-phosphocholine (DSPC) over a series of concentrations and these systems investigated as adjuvants for the delivery of Ag85B-ESAT-6-Rv2660c, a multistage tuberculosis vaccine. METHODS: Liposomal were prepared at a 5 : 1 DDA-TDB weight ratio and DDA content incrementally replaced with DSPC. The physicochemical characteristics were assessed (vesicle size, zeta potential and antigen loading), and the ability of these systems to act as adjuvants was considered. KEY FINDINGS: As DDA was replaced with DSPC within the liposomal formulation, the cationic nature of the vesicles decreases as does electrostatically binding of the anionic H56 antigen (Hybrid56; Ag85B-ESAT6-Rv2660c); however, only when DDA was completed replaced with DSPC did vesicle size increase significantly. T-helper 1 (Th1)-type cell-mediated immune responses reduced. This reduction in responses was attributed to the replacement of DDA with DSPC rather than the reduction in DDA dose concentration within the formulation. CONCLUSION: These results suggest Th1 responses can be controlled by tailoring the DDA/DSPC ratio within the liposomal adjuvant system

Th1 immune responses can be modulated by varying dimethyldioctadecylammonium and distearoyl-sn-glycero-3-phosphocholine content in liposomal adjuvants

Objectives Cationic liposomes of dimethyldioctadecylammonium bromide (DDA) combined with trehalose 6,6′-dibehenate (TDB) elicit strong cell-mediated and antibody immune responses; DDA facilitates antigen adsorption and presentation while TDB potentiates the immune response. To further investigate the role of DDA, DDA was replaced with the neutral lipid of distearoyl-sn- glycero-3-phosphocholine (DSPC) over a series of concentrations and these systems investigated as adjuvants for the delivery of Ag85B-ESAT-6-Rv2660c, a multistage tuberculosis vaccine. Methods Liposomal were prepared at a 5: 1 DDA-TDB weight ratio and DDA content incrementally replaced with DSPC. The physicochemical characteristics were assessed (vesicle size, zeta potential and antigen loading), and the ability of these systems to act as adjuvants was considered. Key findings As DDA was replaced with DSPC within the liposomal formulation, the cationic nature of the vesicles decreases as does electrostatically binding of the anionic H56 antigen (Hybrid56; Ag85B-ESAT6-Rv2660c); however, only when DDA was completed replaced with DSPC did vesicle size increase significantly. T-helper 1 (Th1)-type cell-mediated immune responses reduced. This reduction in responses was attributed to the replacement of DDA with DSPC rather than the reduction in DDA dose concentration within the formulation. Conclusion These results suggest Th1 responses can be controlled by tailoring the DDA/DSPC ratio within the liposomal adjuvant system. © 2013 Royal Pharmaceutical Society

Advances in Ultra Short Pulse Laser based Parallel Processing using a Spatial Light Modulator

AbstractPresented here are latest advances in ultra short pulse laser based parallel processing using a spatial light modulator (SLM), which has the potential for use in high throughput precision patterning of photovoltaic and other device layers. Ultra short laser pulses allow selective material removal with minimal energy density, while here a computer- generated hologram driven reflective SLM is used to transform a single beam into multiple beamlets for increased process throughput. Based on this technique, the precision patterning of silicon, titanium, thin film ITO and metal on flexible and glass substrates is demonstrated and the benefits and current limitations discussed

Developing solid particulate vaccine adjuvants:surface bound antigen favouring a humoural response, whereas entrapped antigen shows a tendency for cell mediated immunity

This present study compares the efficacy of microsphere formulations, and their method of antigen presentation, for the delivery of the TB sub-unit vaccine antigen, Ag85B-ESAT-6. Microspheres based on poly(lactide-co-glycolide) (PLGA) and chitosan incorporating dimethyldioctadecylammonium bromide (DDA) were prepared by either the w/o/w double emulsion method (entrapped antigen) or the o/w single emulsion method (surface bound antigen), and characterised for their physico-chemical properties and their ability to promote an immune response to Ag85B-ESAT-6. The method of preparation, and hence method of antigen association, had a pronounced effect on the type of immune response achieved from the microsphere formulations, with surface bound antigen favouring a humoural response, whereas entrapped antigen favoured a cellular response