Efeito do 2,4D na indução de calogênese in vitro de folhas de erva-mate.

Resumo

The "lessons" of the Australian "heroin shortage"

Heroin use causes considerable harm to individual users including dependence, fatal and nonfatal overdose, mental health problems, and blood borne virus transmission. It also adversely affects the community through drug dealing, property crime and reduced public amenity. During the mid to late 1990s in Australia the prevalence of heroin use increased as reflected in steeply rising overdose deaths. In January 2001, there were reports of an unpredicted and unprecedented reduction in heroin supply with an abrupt onset in all Australian jurisdictions. The shortage was most marked in New South Wales, the State with the largest heroin market, which saw increases in price, dramatic decreases in purity at the street level, and reductions in the ease with which injecting drug users reported being able to obtain the drug. The abrupt onset of the shortage and a subsequent dramatic reduction in overdose deaths prompted national debate about the causes of the shortage and later international debate about the policy significance of what has come to be called the "Australian heroin shortage". In this paper we summarise insights from four years' research into the causes, consequences and policy implications of the "heroin shortage"

Efeito do meio de cultura na calogênese in vitro a partir de folhas de erva-mate.

A organogênese é uma técnica pouco estudada na micropropagação de erva- mate. Este trabalho objetivou avaliar diferentes meios de cultura na calogênese in vitro e organogênese de Ilex paraguariensis St. Hil. Foram coletadas folhas em plantas em casa-de-vegetação. Segmentos foliares foram colocados em meios de cultura 1⁄4 MS, WPM ou JADS, contendo zeatina e 2,4-D. O meio MS foi mais eficiente na indução de calos. No meio WPM foi observada rizogênese. Nos meios testados não houve formação de brotações adventícias.Nota Científica

Role of the Maillard Reaction in Aging of Tissue Proteins: Advanced Glycation End Product-Dependent Increase in Imidazolium Cross-Links in Human Lens Proteins

Dicarbonyl compounds such as glyoxal and methylglyoxal are reactive dicarbonyl intermediates in the nonenzymatic browning and cross-linking of proteins during the Maillard reaction. We describe here the quantification of glyoxal and methylglyoxal-derived imidazolium cross-links in tissue proteins. The imidazolium salt cross-links, glyoxal-lysine dimer (GOLD) and methylglyoxal-lysine dimer (MOLD), were measured by liquid chromatography/mass spectrometry and were present in lens protein at concentrations of 0. 02-0.2 and 0.1-0.8 mmol/mol of lysine, respectively. The lens concentrations of GOLD and MOLD correlated significantly with one another and also increased with lens age. GOLD and MOLD were present at significantly higher concentrations than the fluorescent cross-links pentosidine and dityrosine, identifying them as major Maillard reaction cross-links in lens proteins. Like the N-carboxy-alkyllysines Nepsilon-(carboxymethyl)lysine and Nepsilon-(carboxyethyl)lysine, these cross-links were also detected at lower concentrations in human skin collagen and increased with age in collagen. The presence of GOLD and MOLD in tissue proteins implicates methylglyoxal and glyoxal, either free or protein-bound, as important precursors of protein cross-links formed during Maillard reactions in vivo during aging and in disease

Nova formulação de álcool gel (MicroCelol) com celulose microfibrilada (MFC): avaliação em Escherichia coli.

Durante epidemias ou mais perigosamente nas pandemias, existe uma preocupação da Organizaçao Mundial da Saúde (OMS) e dos profissionais da área de saúde, de uma forma geral, com relação à assepsia de superfícies em geral e das mãos das pessoas, como forma de conter a proliferação dos agentes patogênicos causadores de doenças e, consequentemente, a contaminação das pessoas. Um dos produtos mais usado para a assepsia rápida das mãos e braços é o álcool gel, que é regulamentado no Brasil, pela Agência Nacional de Vigilância Sanitária-Anvisa, a qual tem flexibilizado várias normas e regras para a produção de álcool gel, permitindo o emprego de outros gelificantes (Brasil, 2012, 2020; Anvisa, 2020).bitstream/item/212532/1/CT-444-1798-final-corrigido-1.pd