Biological activity of macromycetes I.

Vyletelka, Juraj. Biological activity of macromycetes I. Master Thesis 2012. Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology. Supervisor: Ing. Kateřina Macáková, PhD. Key words: Ascomycota, Basidiomycota, acetylcholinesterase, butyrylcholinesterase, alkaloids, antioxidant activity, DPPH, ABTS, total phenolics. Macromycetes are the source of bioactive substances that still have not been completely researched. Many of them are used in treatment and they are the object of a growing interest. This work focuses on the measurement of biological activity of 25 ethanolic extracts prepared from various mushroom species. Ellman's spectrophotometric method was used to determine the acetylcholinesterase and butyrylcholinesterase inhibitory activities. The aim of the thin layer chromatography was to detect alkaloids. This work also deals with the determination of antioxidant activity by DPPH and ABTS+ radical scavenging assays modified for use with sequential injection analysis. The content of total phenolic compounds in extracts was determined using the Folin-Ciocaulteu method. Following the results of the presented methods, one may state that the extracts did not exhibit any significant inhibitory activity towards human cholinesterases...

Risk for Major Bleeding in Patients Receiving Ticagrelor Compared With Aspirin After Transient Ischemic Attack or Acute Ischemic Stroke in the SOCRATES Study (Acute Stroke or Transient Ischemic Attack Treated With Aspirin or Ticagrelor and Patient Outcomes)

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