On InChI and evaluating the quality of cross-reference links

BACKGROUND: There are many databases of small molecules focused on different aspects of research and its applications. Some tasks may require integration of information from various databases. However, determining which entries from different databases represent the same compound is not straightforward. Integration can be based, for example, on automatically generated cross-reference links between entries. Another approach is to use the manually curated links stored directly in databases. This study employs well-established InChI identifiers to measure the consistency and completeness of the manually curated links by comparing them with the automatically generated ones. RESULTS: We used two different tools to generate InChI identifiers and observed some ambiguities in their outputs. In part, these ambiguities were caused by indistinctness in interpretation of the structural data used. InChI identifiers were used successfully to find duplicate entries in databases. We found that the InChI inconsistencies in the manually curated links are very high (28.85% in the worst case). Even using a weaker definition of consistency, the measured values were very high in general. The completeness of the manually curated links was also very poor (only 93.8% in the best case) compared with that of the automatically generated links. CONCLUSIONS: We observed several problems with the InChI tools and the files used as their inputs. There are large gaps in the consistency and completeness of manually curated links if they are measured using InChI identifiers. However, inconsistency can be caused both by errors in manually curated links and the inherent limitations of the InChI method

SmSP2: A serine protease secreted by the blood fluke pathogen Schistosoma mansoni with anti-hemostatic properties.

BackgroundSerine proteases are important virulence factors for many pathogens. Recently, we discovered a group of trypsin-like serine proteases with domain organization unique to flatworm parasites and containing a thrombospondin type 1 repeat (TSR-1). These proteases are recognized as antigens during host infection and may prove useful as anthelminthic vaccines, however their molecular characteristics are under-studied. Here, we characterize the structural and proteolytic attributes of serine protease 2 (SmSP2) from Schistosoma mansoni, one of the major species responsible for the tropical infectious disease, schistosomiasis.Methodology/principal findingsSmSP2 comprises three domains: a histidine stretch, TSR-1 and a serine protease domain. The cleavage specificity of recombinant SmSP2 was determined using positional scanning and multiplex combinatorial libraries and the determinants of specificity were identified with 3D homology models, demonstrating a trypsin-like endopeptidase mode of action. SmSP2 displayed restricted proteolysis on protein substrates. It activated tissue plasminogen activator and plasminogen as key components of the fibrinolytic system, and released the vasoregulatory peptide, kinin, from kininogen. SmSP2 was detected in the surface tegument, esophageal glands and reproductive organs of the adult parasite by immunofluorescence microscopy, and in the excretory/secretory products by immunoblotting.Conclusions/significanceThe data suggest that SmSP2 is secreted, functions at the host-parasite interface and contributes to the survival of the parasite by manipulating host vasodilatation and fibrinolysis. SmSP2 may be, therefore, a potential target for anti-schistosomal therapy

New trends in sports training children in football

Bakalářská práce je v teoretické části zaměřena na charakteristiku komplexního biopsychosociálního a fotbalového vývoje dětí v období od 6 do 12 let. Další část práce je věnována změnám ve struktuře a organizaci soutěží dětí od roku 2005 do roku 2013, problematice koučinku a charakteristice nových trendů ve sportovní přípravě dětí ve fotbale.Katedra tělesné a sportovní výchovyObhájenoThe thesis is in the theoretical part focuses on the characteristics of complex biopsychosocial and football development of children in the 6 to 12 years. The next part is devoted to changes in the structure and organization of competitions for children from 2005 to 2013, the issue of coaching and characterization of new trends in sports training of children in football

The origin and development of football in the district Rokycany

Diplomová práce se zabývá vznikem a vývojem fotbalu na Rokycansku. V teoretická část se zaměřuje na obecný vznik fotbalu. Zabývá se následně také vývojem fotbalu na našem území v rámci České republiky a Československa, dále se zaměřuje na vývoj fotbalu na území Plzeňska a západnách Čech a jeho přesun na území okresu Rokycany. V diplomové práci se nachází historický vývoj a vznik jednotlivých klubů v rámci dnešního okresu Rokycany. Diplomová práce je v praktické části obohacena o informace získané pomocí vedení řízených rozhovorů s funkcionáři a pamětníky jednotlivých klubů.ObhájenoThe thesis deals with the formation and development of football in Rokycany. The theoretical part focuses on the general formation of football. It deals with consequently the development of football in our country in the Czech Republic and Czechoslovakia, focusing on the development of football in the Pilsen region and west Bohemia and moving it to the district Rokycany. The thesis is a historic development and the emergence of individual clubs within the district Rokycany. The thesis is the practical part enriched by information obtained through structured interviews with management officials and eyewitnesses individual club

Internal PR communication in telefónica czech republic

Department of Media StudiesKatedra mediálních studiíFakulta sociálních vědFaculty of Social Science

A comparison of approaches to accessing existing biological and chemical relational databases via SPARQL

Abstract Current biological and chemical research is increasingly dependent on the reusability of previously acquired data, which typically come from various sources. Consequently, there is a growing need for database systems and databases stored in them to be interoperable with each other. One of the possible solutions to address this issue is to use systems based on Semantic Web technologies, namely on the Resource Description Framework (RDF) to express data and on the SPARQL query language to retrieve the data. Many existing biological and chemical databases are stored in the form of a relational database (RDB). Converting a relational database into the RDF form and storing it in a native RDF database system may not be desirable in many cases. It may be necessary to preserve the original database form, and having two versions of the same data may not be convenient. A solution may be to use a system mapping the relational database to the RDF form. Such a system keeps data in their original relational form and translates incoming SPARQL queries to equivalent SQL queries, which are evaluated by a relational-database system. This review compares different RDB-to-RDF mapping systems with a primary focus on those that can be used free of charge. In addition, it compares different approaches to expressing RDB-to-RDF mappings. The review shows that these systems represent a viable method providing sufficient performance. Their real-life performance is demonstrated on data and queries coming from the neXtProt project

Metody strukturované analýzy a návrhu informačních systémů

Práce je zaměřena na seznámení s vývojem informačních systémů pomocí standardizovaných metodických postupů pro analýzu a návrh. V práci jsou dále představeny základní strukturované metodiky užívané pro vývoj informačních systémů ve světě a přiblížena situace v této oblasti panující v současné době v České republice. V závěru je provedeno srovnání metodik a postupů a ukázáno několik doporučení pro výběr toho nejvhodnějšího postupu

Metody strukturované analýzy a návrhu informačních systémů

Práce je zaměřena na seznámení s vývojem informačních systémů pomocí standardizovaných metodických postupů pro analýzu a návrh. V práci jsou dále představeny základní strukturované metodiky užívané pro vývoj informačních systémů ve světě a přiblížena situace v této oblasti panující v současné době v České republice. V závěru je provedeno srovnání metodik a postupů a ukázáno několik doporučení pro výběr toho nejvhodnějšího postupu