99 research outputs found
Theory of excitons in cubic III-V semiconductor GaAs, InAs and GaN quantum dots: fine structure and spin relaxation
Exciton fine structures in cubic III-V semiconductor GaAs, InAs and GaN
quantum dots are investigated systematically and the exciton spin relaxation in
GaN quantum dots is calculated by first setting up the effective exciton
Hamiltonian. The electron-hole exchange interaction Hamiltonian, which consists
of the long- and short-range parts, is derived within the effective-mass
approximation by taking into account the conduction, heavy- and light-hole
bands, and especially the split-off band. The scheme applied in this work
allows the description of excitons in both the strong and weak confinement
regimes. The importance of treating the direct electron-hole Coulomb
interaction unperturbatively is demonstrated. We show in our calculation that
the light-hole and split-off bands are negligible when considering the exciton
fine structure, even for GaN quantum dots, and the short-range exchange
interaction is irrelevant when considering the optically active doublet
splitting. We point out that the long-range exchange interaction, which is
neglected in many previous works, contributes to the energy splitting between
the bright and dark states, together with the short-range exchange interaction.
Strong dependence of the optically active doublet splitting on the anisotropy
of dot shape is reported. Large doublet splittings up to 600 eV, and even
up to several meV for small dot size with large anisotropy, is shown in GaN
quantum dots. The spin relaxation between the lowest two optically active
exciton states in GaN quantum dots is calculated, showing a strong dependence
on the dot anisotropy. Long exciton spin relaxation time is reported in GaN
quantum dots. These findings are in good agreement with the experimental
results.Comment: 22+ pages, 16 figures, several typos in the published paper are
corrected in re
Plant extracts in cell-based anti-inflammatory assaysâPitfalls and considerations related to removal of activity masking bulk components
Plants used in traditional medicine represent an important source of new lead compounds. However, cell-based in vitro screening assays with plant material are hampered by the complex nature of plant extracts as mixtures of active and inactive components. Bulk constituents, such as chlorophyll and polyphenols were previously shown to interfere with several biological in vitro assays. Their influence on anti-inflammatory cell-based testing systems has not been thoroughly investigated. Hence, the present study was aimed at comparing different procedures for the removal of bulk constituents from plant extracts and examining the influence of their elimination on selected cell-based anti-inflammatory assays.
Malva sp. and Glechoma hederacea L., two plants used in traditional European medicine for the treatment of inflammatory disorders, were subjected to three different methods for the removal of chlorophyll and polyphenols, respectively. Removal of bulk constituents was confirmed by HPLC and mass spectrometry. Extracts were tested before and after the purification procedure, to determine their potential to inhibit the activation of the transcription factor NF-ÎșB in reporter gene assay and to interfere with the secretion of the chemokine IL-8 after stimulation of endothelial cells with tumor necrosis factor (TNF-α) or lipopolysaccharide (LPS). Removal of chlorophyll from tested extracts led to a strong decrease in the anti-inflammatory activities, due to loss of bioactive constituents. In contrast, the effect of the polyphenol-free extracts was either not changed or significantly increased, depending on the purification method used. The study concluded that clearance of bulk compounds represents a valuable strategy for cell-based in vitro anti-inflammatory evaluation of plant extracts. Liquidâliquid partitioning was identified as the optimal method for the elimination of both chlorophyll and polyphenols. It is recommended that removal of chlorophyll from extracts always be accompanied by HPLC profiling to detect a possible loss of active constituents
Association of polygenic score and the involvement of cholinergic and glutamatergic pathways with lithium treatment response in patients with bipolar disorder
Lithium is regarded as the first-line treatment for bipolar disorder (BD), a severe and disabling mental health disorder that affects about 1% of the population worldwide. Nevertheless, lithium is not consistently effective, with only 30% of patients showing a favorable response to treatment. To provide personalized treatment options for bipolar patients, it is essential to identify prediction biomarkers such as polygenic scores. In this study, we developed a polygenic score for lithium treatment response (Li+PGS) in patients with BD. To gain further insights into lithiumâs possible molecular mechanism of action, we performed a genome-wide gene-based analysis. Using polygenic score modeling, via methods incorporating Bayesian regression and continuous shrinkage priors, Li+PGS was developed in the International Consortium of Lithium Genetics cohort (ConLi+Gen: N = 2367) and replicated in the combined PsyCourse (N = 89) and BipoLife (N = 102) studies. The associations of Li+PGS and lithium treatment response â defined in a continuous ALDA scale and a categorical outcome (good response vs. poor response) were tested using regression models, each adjusted for the covariates: age, sex, and the first four genetic principal components. Statistical significance was determined at P < 0.05. Li+PGS was positively associated with lithium treatment response in the ConLi+Gen cohort, in both the categorical (P = 9.8 Ă 10â12, R2 = 1.9%) and continuous (P = 6.4 Ă 10â9, R2 = 2.6%) outcomes. Compared to bipolar patients in the 1st decile of the risk distribution, individuals in the 10th decile had 3.47-fold (95%CI: 2.22â5.47) higher odds of responding favorably to lithium. The results were replicated in the independent cohorts for the categorical treatment outcome (P = 3.9 Ă 10â4, R2 = 0.9%), but not for the continuous outcome (P = 0.13). Gene-based analyses revealed 36 candidate genes that are enriched in biological pathways controlled by glutamate and acetylcholine. Li+PGS may be useful in the development of pharmacogenomic testing strategies by enabling a classification of bipolar patients according to their response to treatment
Gut mucosal DAMPs in IBD: From mechanisms to therapeutic implications
Endogenous damage-associated molecular patterns (DAMPs) are released during tissue damage and have increasingly recognized roles in the etiology of many human diseases. The inflammatory bowel diseases (IBD), ulcerative colitis (UC) and Crohnâs disease (CD), are immune-mediated conditions where high levels of DAMPs are observed. DAMPs such as calprotectin (S100A8/9) have an established clinical role as a biomarker in IBD. In this review, we use IBD as an archetypal common chronic inflammatory disease to focus on the conceptual and evidential importance of DAMPs in pathogenesis and why DAMPs represent an entirely new class of targets for clinical translation. </p
LEGEND-1000 Preconceptual Design Report
We propose the construction of LEGEND-1000, the ton-scale Large Enriched Germanium Experiment for Neutrinoless Decay. This international experiment is designed to answer one of the highest priority questions in fundamental physics. It consists of 1000 kg of Ge detectors enriched to more than 90% in the Ge isotope operated in a liquid argon active shield at a deep underground laboratory. By combining the lowest background levels with the best energy resolution in the field, LEGEND-1000 will perform a quasi-background-free search and can make an unambiguous discovery of neutrinoless double-beta decay with just a handful of counts at the decay value. The experiment is designed to probe this decay with a 99.7%-CL discovery sensitivity in the Ge half-life of years, corresponding to an effective Majorana mass upper limit in the range of 9-21 meV, to cover the inverted-ordering neutrino mass scale with 10 yr of live time
True real-time cardiac MRI in free breathing without ECG synchronization using a novel sequence with radial k-space sampling and balanced SSFP contrast mode.
We investigated a novel sequence with radial k-space sampling, gridding and sliding window reconstruction with bSSFP contrast that allows for true real-time functional cardiac evaluation independent from respiration and ECG triggering. 12 healthy volunteers underwent 1.5 T cardiac MRI. Single-shot short axis views were acquired with a) standard retrospectively ECG-gated segmented breath-hold (bh) bSSFP and with the real-time radial bSSFP sequence with a nominal temporal resolution of b) 16 fps (frames per second) and c) 40 fps. Radial bSSFP were acquired during free breathing without ECG synchronization. Left ventricular functional parameters (EDV, ESV, SV and EF) were compared and quality of wall motion depiction was assessed. Contrast-to-noise-ratio (CNR) of myocardium/blood pool in the left ventricle was calculated. EF showed excellent correlation (Bland-Altman r = 0.99; Lin rho = 0.91) between bh-bSSFP (65 %) and 40 fps radial (64 %) and moderate correlation (r = 0.84, rho = 0.20) with 16 fps radial bSSFP (56 %). While EDV was in good agreement for all three sequences, ESV was significantly overestimated with 16 fps radial bSSFP. Despite lower CNR, image quality for wall motion assessment was rated significantly better for 40 fps compared to 16 fps radial bSSFP due to the faster temporal resolution. Left ventricular functional analysis with fast true real-time radial bSSFP is in good agreement with standard ECG-gated bh-bSSFP. The independency from ECG synchronization and breathing promises a robust method for patients with impaired cardiopulmonary status in whom breath-hold and good quality ECG cannot be achieved
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