The cyanobacterial endosymbiont of the unicellular algae Rhopalodia gibba shows reductive genome evolution

<p>Abstract</p> <p>Background</p> <p>Bacteria occur in facultative association and intracellular symbiosis with a diversity of eukaryotic hosts. Recently, we have helped to characterise an intracellular nitrogen fixing bacterium, the so-called spheroid body, located within the diatom <it>Rhopalodia gibba</it>. Spheroid bodies are of cyanobacterial origin and exhibit features that suggest physiological adaptation to their intracellular life style. To investigate the genome modifications that have accompanied the process of endosymbiosis, here we compare gene structure, content and organisation in spheroid body and cyanobacterial genomes.</p> <p>Results</p> <p>Comparison of the spheroid body's genome sequence with corresponding regions of near free-living relatives indicates that multiple modifications have occurred in the endosymbiont's genome. These include localised changes that have led to elimination of some genes. This gene loss has been accompanied either by deletion of the respective DNA region or replacement with non-coding DNA that is AT rich in composition. In addition, genome modifications have led to the fusion and truncation of genes. We also report that in the spheroid body's genome there is an accumulation of deleterious mutations in genes for cell wall biosynthesis and processes controlled by transposases. Interestingly, the formation of pseudogenes in the spheroid body has occurred in the presence of intact, and presumably functional, <it>rec</it>A and <it>rec</it>F genes. This is in contrast to the situation in most investigated obligate intracellular bacterium-eukaryote symbioses, where at least either <it>rec</it>A or <it>rec</it>F has been eliminated.</p> <p>Conclusion</p> <p>Our analyses suggest highly specific targeting/loss of individual genes during the process of genome reduction and establishment of a cyanobacterial endosymbiont inside a eukaryotic cell. Our findings confirm, at the genome level, earlier speculation on the obligate intracellular status of the spheroid body in <it>Rhopalodia gibba</it>. This association is the first example of an obligate cyanobacterial symbiosis involving nitrogen fixation for which genomic data are available. It represents a new model system to study molecular adaptations of genome evolution that accompany a switch from free-living to intracellular existence.</p

EXPANSIN A1-mediated radial swelling of pericycle cells positions anticlinal cell divisions during lateral root initiation

In plants, postembryonic formation of new organs helps shape the adult organism. This requires the tight regulation of when and where a new organ is formed and a coordination of the underlying cell divisions. To build a root system, new lateral roots are continuously developing, and this process requires the tight coordination of asymmetric cell division in adjacent pericycle cells. We identified EXPANSIN A1 (EXPA1) as a cell wall modifying enzyme controlling the divisions marking lateral root initiation. Loss of EXPA1 leads to defects in the first asymmetric pericycle cell divisions and the radial swelling of the pericycle during auxin-driven lateral root formation. We conclude that a localized radial expansion of adjacent pericycle cells is required to position the asymmetric cell divisions and generate a core of small daughter cells, which is a prerequisite for lateral root organogenesis

Stable nitrogen isotopes in coastal macroalgae: geographic and anthropogenic variability

Proyectos ANILE (CTM2009-08396 and CTM2010-08804-E) del Plan Nacional de I+D+i y RADIALES del Instituto Español de Oceanografía (IEO). I.G.V. recibió un contrato FPI del Ministerio de Economía y CompetividadGrowing human population add to the natural nitrogen loads to coastal waters. As the excess nitrogen is readily incorporated in new biomass anthropogenic and natural nitrogen sources may be traced by the measurement of stable nitrogen isotopes (δ15N). In this study δ15N was determined in two species of macroalgae (Ascophyllum nodosum and Fucus vesiculosus), and in nitrate and ammonium to determine the relative importance of anthropogenic versus natural sources of nitrogen along the coast of NW Spain. Both algal species and nitrogen sources showed similar isotopic enrichment for a given site, but algal δ15N was not related to either inorganic nitrogen concentrations or δ15N in the water samples. The latter suggests that inorganic nitrogen inputs are variable and do not always leave an isotopic trace in macroalgae. However, a significant linear decrease in macroalgal δ15N along the coast is consistent with the differential effect of upwelling. Besides this geographic variability, the influence of anthropogenic nitrogen sources is evidenced by higher δ15N in macroalgae from rias and estuaries compared to those from open coastal areas and in areas with more than 15x103 inhabitants in the watershed. These results indicate that, in contrast with other studies, macroalgal δ15Nis not simply related to either inorganic nitrogen concentrations or human population size but depends on other factors as the upwelling or the efficiency of local waste treatment systems.Plan nacional I+D+i, IEOPreprint3,258

Drought at the global scale for the 2nd part of the 20th Century (1963-2001)

The large impacts of drought on society, economy and environment urge for a thorough investigation. A good knowledge of past drought events is important for both understanding of the processes causing drought, as well as to provide reliability assessments for drought projections for the future. Preferably, the investigation of historic drought events should rely on observations. Unfortunately, for a global scale these detailed observations are often not available. Therefore, the outcome of global hydrological models (GHMs) and o-line land surface models (LSMs) is used to assess droughts. In this study we have investigated to what extent simulated gridded time series from these large-scale models capture historic hydrological drought events. Results of ten dierent models, both GHMs and LSMs, made available by the WATCH project, were compared. All models are run on a global 0.5 grid for the period 1963-2000 with the same meteorological forcing data (WATCH forcing data). To identify hydrological drought events, the monthly aggregated total runo values were used. Dierent methods were developed to identify spatio-temporal drought characteristics. General drought characteristics for each grid cell, as for example the average drought duration, were compared. These characteristics show that when comparing absolute values the models give substantially dierent results, whereas relative values lead to more or less the same drought pattern. Next to the general drought characteristics, some documented major historical drought events (one for each continent) were selected and described in more detail. For each drought event, the simulated drought clusters (spatial events) and their characteristics are given for one month during the event. It can be concluded that most major drought events are captured by all models. However, the spatial extent of the drought events dier substantially between the models. In general the models show a fast reaction to rainfall and therefore also capture drought events caused by large rainfall anomalies. More research is still needed, since here we only looked at a few selected number of documented drought events spread over the globe. To assess more in detail if these large-scale models are able to capture drought, additional quantitative analyses are needed together with a more elaborated comparison against observed drought event