Questionnaire assessment of the welfare of pigs

A questionnaire study was performed involving 76 farms with a total of 380,207 pigs to assess the welfare of pigs kept in Hungary based on the animal welfare legislation of the European Union. Most significant deficiencies were found in the fulfilment of the provisions relating to space requirements, the stall microclimate and the behavioural needs of the animals

The Extreme Energy Events HECR array: status and perspectives

The Extreme Energy Events Project is a synchronous sparse array of 52 tracking detectors for studying High Energy Cosmic Rays (HECR) and Cosmic Rays-related phenomena. The observatory is also meant to address Long Distance Correlation (LDC) phenomena: the network is deployed over a broad area covering 10 degrees in latitude and 11 in longitude. An overview of a set of preliminary results is given, extending from the study of local muon flux dependance on solar activity to the investigation of the upward-going component of muon flux traversing the EEE stations; from the search for anisotropies at the sub-TeV scale to the hints for observations of km-scale Extensive Air Shower (EAS).Comment: XXV ECRS 2016 Proceedings - eConf C16-09-04.

A molecular cascade modulates MAP1B and confers resistance to mTOR inhibition in human glioblastoma.

BackgroundClinical trials of therapies directed against nodes of the signaling axis of phosphatidylinositol-3 kinase/Akt/mammalian target of rapamycin (mTOR) in glioblastoma (GBM) have had disappointing results. Resistance to mTOR inhibitors limits their efficacy.MethodsTo determine mechanisms of resistance to chronic mTOR inhibition, we performed tandem screens on patient-derived GBM cultures.ResultsAn unbiased phosphoproteomic screen quantified phosphorylation changes associated with chronic exposure to the mTOR inhibitor rapamycin, and our analysis implicated a role for glycogen synthase kinase (GSK)3B attenuation in mediating resistance that was confirmed by functional studies. A targeted short hairpin RNA screen and further functional studies both in vitro and in vivo demonstrated that microtubule-associated protein (MAP)1B, previously associated predominantly with neurons, is a downstream effector of GSK3B-mediated resistance. Furthermore, we provide evidence that chronic rapamycin induces microtubule stability in a MAP1B-dependent manner in GBM cells. Additional experiments explicate a signaling pathway wherein combinatorial extracellular signal-regulated kinase (ERK)/mTOR targeting abrogates inhibitory phosphorylation of GSK3B, leads to phosphorylation of MAP1B, and confers sensitization.ConclusionsThese data portray a compensatory molecular signaling network that imparts resistance to chronic mTOR inhibition in primary, human GBM cell cultures and points toward new therapeutic strategies

Serum-free culture success of glial tumors is related to specific molecular profiles and expression of extracellular matrix–associated gene modules

BACKGROUND: Recent molecular characterization studies have identified clinically relevant molecular subtypes to coexist within the same histological entities of glioma. Comparative studies between serum-supplemented and serum-free (SF) culture conditions have demonstrated that SF conditions select for glioma stem-like cells, which superiorly conserve genomic alterations. However, neither the representation of molecular subtypes within SF culture assays nor the molecular distinctions between successful and nonsuccessful attempts have been elucidated. METHODS: A cohort of 261 glioma samples from varying histological grades was documented for SF culture success and clinical outcome. Gene expression and single nucleotide polymorphism arrays were interrogated on a panel of tumors for comparative analysis of SF+ (successful cultures) and SF− (unsuccessful cultures). RESULTS: SF culture outcome was correlated with tumor grade, while no relation was found between SF+ and patient overall survival. Copy number–based hierarchical clustering revealed an absolute separation between SF+ and SF− parental tumors. All SF+ cultures are derived from tumors that are isocitrate dehydrogenase 1 (IDH1) wild type, chromosome 7 amplified, and chromosome 10q deleted. SF− cultures derived from IDH1 mutant tumors demonstrated a fade-out of mutated cells during the first passages. SF+ tumors were enriched for The Cancer Genome Atlas Classical subtype and intrinsic glioma subtype-18. Comparative gene ontology analysis between SF+ and SF− tumors demonstrated enrichment for modules associated with extracellular matrix composition, Hox-gene signaling, and inflammation. CONCLUSION: SF cultures are derived from a subset of parental tumors with a shared molecular background including enrichment for extracellular matrix–associated gene modules. These results provide leads to develop enhanced culture protocols for glioma samples not propagatable under current SF conditions

Recent results and performance of the multi-gap resistive plate chambers network for the EEE Project

The Extreme Energy Events (EEE) Project is devoted to the study of Extensive Atmospheric Showers through a network of muon telescopes, installed in High Schools, with the further aim of introducing young students to particle and astroparticle physics. Each telescope is a tracking detector composed of three Multi-gap Resistive Plate Chambers (MRPC) with an active area of 1.60 × 0.80 m(2). Their characteristics are similar to the ones built for the Time Of Flight array of the ALICE Experimentat LHC . The EEE Project started with a few pilot towns, where the telescopes have been taking data since 2008, and it has been constantly extended, reaching at present more than 50 MRPCs telescopes. They are spread across Italy with two additional stations at CERN, covering an area of around 3 × 10(5) km(2), with a total surface area for all the MRPCs of 190 m(2). A comprehensive description of the MRPCs network is reported here: efficiency, time and spatial resolution measured using cosmic rays hitting the telescopes. The most recent results on the detector and physics performance from a series of coordinated data acquisition periods are also presented

The EEE Project: a sparse array of telescopes for the measurement of cosmic ray muons

The Extreme Energy Events (EEE) Project is meant to be the most extensive experiment to detect secondary cosmic particles in Italy. To this aim, more than 50 telescopes have been built at CERN and installed in high schools distributed all over the Italian territory. Each EEE telescope comprises three large area Multigap Resistive Plate Chambers (MRPCs) and is capable of reconstructing the trajectories of the charged particles traversing it with a good angular resolution. The excellent performance of the EEE telescopes allows a large variety of studies, from measuring the local muon flux in a single telescope, to detecting extensive air showers producing time correlations in the same metropolitan area, to searching for large-scale correlations between showers detected in telescopes tens, hundreds or thousands of kilometers apart. In addition to its scientific goal, the EEE Project also has an educational and outreach objective, its aim being to motivate young people by involving them directly in a real experiment. High school students and teachers are involved in the construction, testing and start-up of the EEE telescope in their school, then in its maintenance and data-acquisition, and later in the analysis of the data. During the last couple of years a great boost has been given to the EEE Project through the organization of simultaneous and centralized data taking with the whole telescope array. The raw data from all telescopes are transferred to CNAF (Bologna), where they are reconstructed and stored. The data are currently being analyzed, looking at various topics: variation of the rate of cosmic muons with time, upward going muons, muon lifetime, search for anisotropies in the muon angular distribution and for time coincidences between stations. In this paper an overall description of the experiment is given, including the design, construction and performance of the telescopes. The operation of the whole array is also presented by showing the most recent physics results