3,272 research outputs found
Epidemics on contact networks: a general stochastic approach
Dynamics on networks is considered from the perspective of Markov stochastic
processes. We partially describe the state of the system through network motifs
and infer any missing data using the available information. This versatile
approach is especially well adapted for modelling spreading processes and/or
population dynamics. In particular, the generality of our systematic framework
and the fact that its assumptions are explicitly stated suggests that it could
be used as a common ground for comparing existing epidemics models too complex
for direct comparison, such as agent-based computer simulations. We provide
many examples for the special cases of susceptible-infectious-susceptible (SIS)
and susceptible-infectious-removed (SIR) dynamics (e.g., epidemics propagation)
and we observe multiple situations where accurate results may be obtained at
low computational cost. Our perspective reveals a subtle balance between the
complex requirements of a realistic model and its basic assumptions.Comment: Main document: 16 pages, 7 figures. Electronic Supplementary Material
(included): 6 pages, 1 tabl
Neglect of Medical Evidence of Torture in Guantánamo Bay: A Case Series
Vincent Iacopino and Stephen Xenakis review case records of nine individuals
imprisoned at Guantánamo Bay which indicate that medical personnel assigned to
the US Department of Defense neglected and/or concealed medical evidence of
torture
Activation of Notch signalling by soluble Dll4 decreases vascular permeability via a cAMP/PKA-dependent pathway
© 2019 the American Physiological Society. The Notch ligand delta-like ligand 4 (Dll4), upregulated by VEGF, is a key regulator of vessel morphogenesis and function, controlling tip and stalk cell selection during sprouting angiogenesis. Inhibition of Dll4 results in hypersprouting, nonfunctional, poorly perfused vessels, suggesting a role for Dll4 in the formation of mature, reactive, functional vessels, with low permeability and able to restrict fluid and solute exchange. We tested the hypothesis that Dll4 controls transvascular fluid exchange. A recombinant protein expressing only the extracellular portion of Dll4 [soluble Dll4 (sDll4)] induced Notch signaling in endothelial cells (ECs), resulting in increased expression of vascular-endothelial cadherin, but not the tight junctional protein zonula occludens 1, at intercellular junctions. sDll4 decreased the permeability of FITC-labeled albumin across EC monolayers, and this effect was abrogated by coculture with the γ-secretase inhibitor N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester. One of the known molecular effectors responsible for strengthening EC-EC contacts is PKA, so we tested the effect of modulation of PKA on the sDll4-mediated reduction of permeability. Inhibition of PKA reversed the sDll4-mediated reduction in permeability and reduced expression of the Notch target gene Hey1. Knockdown of PKA reduced sDLL4-mediated vascular-endothelial cadherin junctional expression. sDll4 also caused a significant decrease in the hydraulic conductivity of rat mesenteric microvessels in vivo. This reduction was abolished upon coperfusion with the PKA inhibitor H89 dihydrochloride. These results indicate that Dll4 signaling through Notch activation acts through a cAMP/PKA pathway upon intercellular adherens junctions, but not tight junctions, to regulate endothelial barrier function. NEW & NOTEWORTHY Notch signaling reduces vascular permeability through stimulation of cAMP-dependent protein kinase A
Dark-adapted red flash ERGs in healthy adults
Purpose: The x-wave of the dark-adapted (DA) ERG to a red flash reflects DA cone function. This exploratory study of healthy adults aimed to investigate changes in the DA red ERG with flash strength and during dark adaptation to optimise visualisation and therefore quantification of the x-wave.
Methods: The effect of altering red flash strength was investigated in four subjects by recording ERGs after 20 minutes dark adaptation to red flashes (0.2–2.0 cd s m-2) using skin electrodes and natural pupils. The effect of dark adaptation duration was investigated in 16 subjects during 20 minutes in the dark, by recording DA 1.5 red ERGs at 1, 2, 3, 4, 5, 10, 15 and 20 minutes.
Results: For a dark adaption period of 20 minutes, the x-wave was more clearly visualised to weaker (< 0.6 cd s m-2) red flash strengths: to stronger flashes it became obscured by the b-wave. For red flashes of 1.5 cd s m-2, the x-wave was most prominent in ERGs recorded after 1–5 minutes of dark adaptation: with longer dark-adaptation, it was subsumed into the b-wave’s rising edge.
Conclusions: This small study suggests that x-wave visibility in healthy subjects after 20 minutes dark adaptation is improved by using flashes weaker than around 0.6 cd s m-2; for flash strengths of 1.5 cd s m-2, x-wave visibility is enhanced by recording after only around 5 minutes of dark adaptation. No evidence was found that interim red flash ERGs affecting the dark-adapted state of the normal retina
Recognition without identification, erroneous familiarity, and déjà vu
Déjà vu is characterized by the recognition of a situation concurrent with the awareness that this recognition is inappropriate. Although forms of déjà vu resolve in favor of the inappropriate recognition and therefore have behavioral consequences, typical déjà vu experiences resolve in favor of the awareness that the sensation of recognition is inappropriate. The resultant lack of behavioral modification associated with typical déjà vu means that clinicians and experimenters rely heavily on self-report when observing the experience. In this review, we focus on recent déjà vu research. We consider issues facing neuropsychological, neuroscientific, and cognitive experimental frameworks attempting to explore and experimentally generate the experience. In doing this, we suggest the need for more experimentation and amore cautious interpretation of research findings, particularly as many techniques being used to explore déjà vu are in the early stages of development.PostprintPeer reviewe
Segond's fracture: a biomechanical cadaveric study using navigation
Background Segond’s fracture is a well-recognised radiological
sign of an anterior cruciate ligament (ACL) tear.
While previous studies evaluated the role of the anterolateral
ligament (ALL) and complex injuries on rotational
stability of the knee, there are no studies on the biomechanical
effect of Segond’s fracture in an ACL deficient
knee. The aim of this study was to evaluate the effect of a
Segond’s fracture on knee rotation stability as evaluated by
a navigation system in an ACL deficient knee.
Materials and methods Three different conditions were
tested on seven knee specimens: intact knee, ACL deficient
knee and ACL deficient knee with Segond’s fracture. Static
and dynamic measurements of anterior tibial translation
(ATT) and axial tibial rotation (ATR) were recorded by the
navigation system (2.2 OrthoPilot ACL navigation system
B. Braun Aesculap, Tuttlingen, Germany).
Results Static measurements at 30 showed that the mean
ATT at 30 of knee flexion was 5.1 ± 2.7 mm in the ACL
intact condition, 14.3 ± 3.1 mm after ACL cut
(P = 0.005), and 15.2 ± 3.6 mm after Segond’s fracture
(P = 0.08). The mean ATR at 30 of knee flexion was
20.7 ± 4.8 in the ACL intact condition, 26.9 ± 4.1 in
the ACL deficient knee (P[0.05) and 30.9 ± 3.8 after
Segond’s fracture (P = 0.005). Dynamic measurements
during the pivot-shift showed that the mean ATT was
7.2 ± 2.7 mm in the intact knee, 9.1 ± 3.3 mm in the
ACL deficient knee(P = 0.04) and 9.7 ± 4.3 mm in the
ACL deficient knee with Segond’s fracture (P = 0.07).
The mean ATR was 9.6 ± 1.8 in the intact knee,
12.3 ± 2.3 in the ACL deficient knee (P[0.05) and
19.1 ± 3.1 in the ACL deficient knee with Segond’s
lesion (P = 0.016).
Conclusion An isolated lesion of the ACL only affects
ATT during static and dynamic measurements, while the
addition of Segond’s fracture has a significant effect on
ATR in both static and dynamic execution of the pivot-shift
test, as evaluated with the aid of navigation
Surface topography of hydroxyapatite affects ROS17/2.8 cells response
Hydroxyapatite (HA) has been used in orthopedic, dental, and maxillofacial surgery as a bone substitute.
The aim of this investigation was to study the effect of surface topography produced by the presence of microporosity on cell response, evaluating: cell attachment, cell morphology, cell proliferation, total protein content, and alkaline phosphatase (ALP) activity. HA discs with different percentages of microporosity (< 5%, 15%, and 30%) were confected by means of the combination of uniaxial powder pressing and different sintering conditions. ROS17/2.8 cells were cultured
on HA discs. For the evaluation of attachment, cells were cultured for two hours. Cell morphology was evaluated
after seven days. After seven and fourteen days, cell proliferation, total protein content, and ALP activity were measured. Data were compared by means of ANOVA and Duncan’s multiple range test, when appropriate. Cell attachment (p = 0.11) and total protein content (p = 0.31) were not affected by surface topography. Proliferation after 7 and 14 days (p = 0.0007 and p = 0.003, respectively), and ALP activity (p = 0.0007) were both significantly decreased by the most irregular surface (HA30). These results suggest that initial cell events were not affected by surface topography, while surfaces with more regular topography, as those present in HA with 15% or less of microporosity, favored intermediary and final events such as cell proliferation and ALP activity
Evaluation of Systematic Assessment of Asthma-Like Symptoms and Tobacco Smoke Exposure in Early Childhood by Well-Child Professionals: A Randomised Trial
Heavy quark flavour dependence of multiparticle production in QCD jets
After inserting the heavy quark mass dependence into QCD partonic evolution
equations, we determine the mean charged hadron multiplicity and second
multiplicity correlators of jets produced in high energy collisions. We thereby
extend the so-called dead cone effect to the phenomenology of multiparticle
production in QCD jets and find that the average multiplicity of heavy-quark
initiated jets decreases significantly as compared to the massless case, even
taking into account the weak decay products of the leading primary quark. We
emphasize the relevance of our study as a complementary check of -tagging
techniques at hadron colliders like the Tevatron and the LHC.Comment: Version revised, accepted for publication in JHEP, 21 pages and 7
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