Palabras llenas de fronteras: Arnaldo Calveyra y su Diario francés (1959-1960). Vivir a través de cristal

En el libro se lee: “Palabras llenas de fronteras […] difícilmente encastran en la experiencia”, y resultan pertinentes para comenzar, por dos motivos; uno, porque las de Calveyra están siempre entre la frontera del verso y la prosa, el drama y la comedia, el francés y el castellano; y, dos, porque Diario francés (1959 -1960) difícilmente encastre en la experiencia de los anteriores diarios escritos por el mismo autor o en la forma clásica de los diarios de bitácora. Por esta última razón el ..

Nodulation competitiveness and diversification of symbiosis genes in common beans from the American centers of domestication

Phaseolus vulgaris (common bean), having a proposed Mexican origin within the Americas, comprises three centers of diversification: Mesoamerica, the southern Andes, and the Amotape-Huancabamba Depression in Peru-Ecuador. Rhizobium etli is the predominant rhizobium found symbiotically associated with beans in the Americasalthough closely related Rhizobium phylotypes have also been detected. To investigate if symbiosis between bean varieties and rhizobia evolved affinity, firstly nodulation competitiveness was studied after inoculation with a mixture of sympatric and allopatric rhizobial strains isolated from the respective geographical regions. Rhizobia strains harboring nodC types α and γ , which were found predominant in Mexico and Ecuador, were comparable in nodule occupancy at 50% of each in beans from the Mesoamerican and Andean gene pools, but it is one of those two nodC types which clearly predominated in Ecuadorian-Peruvian beans as well as in Andean beans nodC type γ predominated the sympatric nodC type δ. The results indicated that those beans from Ecuador-Peru and Andean region, respectively exhibited no affinity for nodulation by the sympatric rhizobial lineages that were found to be predominant in bean nodules formed in those respective areas. Unlike the strains isolated from Ecuador, Rhizobium etli isolated from Mexico as well from the southern Andes was highly competitive for nodulation in beans from Ecuador-Peru, and quite similarly competitive in Mesoamerican and Andean beans. Finally, five gene products associated with symbiosis were examined to analyze variations that could be correlated with nodulation competitiveness. A small GTPase RabA2, transcriptional factors NIN and ASTRAY, and nodulation factor receptors NFR1 and NFR5- indicated high conservation but NIN, NFR1 and NFR5 of beans representative of the Ecuador-Peru genetic pool clustered separated from the Mesoamerican and Andean showing diversification and possible different interaction. These results indicated that both host and bacterial genetics are important for mutual affinity, and that symbiosis is another trait of legumes that could be sensitive to evolutionary influences and local adaptation.Fil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Collavino, Mónica Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Mancini Villagra, Ulises Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentin

In-service teachers and attention to students with physical disability: training, concerns and needs

The purpose of the study was to delve into the training of an in-service Physical Education Teachers (PET) in schools where students with a physical disability attend, their concerns and needs, as well as the relationship with other professionals. The participants were 57 in-service teachers and 3 physiotherapists (PT). The data were gathered from questionnaires from all the teachers and an interview of 8 PET and 3 PT. The results indicate they need to improve their training, and, in varying degrees, in such areas as certain sports, learning tasks and curriculum adaptations. The study reveals PT ensure that exercises are conducted which benefit students with disability and not those types which are designed for non-disabled students. Furthermore, teachers’ perception of a lack of training results in feeling unable to deal with students with disability, and becoming dependent on the PT

COORDENAÇÃO ENTRE PROFESSORES DE EDUCAÇÃO FÍSICA E FISIOTERAPEUTA DURANTE AULAS DE EDUCAÇÃO FÍSICA: O CASO DA COMUNIDADE AUTÔNOMA DE MADRID NA ESPANHA

The purpose of this study was to determine and analyse Physical Education (PE) teachers’ perceptions about inclusion of students with physical disabilities in regular PE classes and how such inclusion could be improved by the contribution of other professionals. The information was obtained through a questionnaire administered to 56 PE teachers and an interview with nine PE teachers and four physical therapists. The results reveal PE teachers’ concern and uncertainty about providing quality attention to students with physical disabilities in their classes and their demand for specific training and support in class. They see the need for support from professionals such as Physical Therapists’ when working with students with motor disabilities. We suggest further examination of the barriers faced by both professionals, their views on PE teachers and the physical therapists’ role, as well as their willingness to collaborate with each other. El objetivo del estudio fue determinar y analizar las percepciones del profesorado de educación física que imparte clases al alumnado con discapacidad en las clases inclusivas de Educación Física y cómo mejorar esa inclusión cuando se colabora con el fisioterapeuta. Se obtuvo la información mediante un cuestionario administrado a 56 profesores, que se completó con entrevistas a nueve de ellos y a cuatro fisioterapeutas. Los resultados muestran la preocupación e incertidumbre del profesorado sobre cómo atender al alumnado con discapacidad para poder ofrecer una educación inclusiva de calidad y la demanda de capacitación y apoyos específicos en sus clases. El profesorado considera necesario el apoyo del fisioterapeuta como profesional adecuado para ayudar al alumnado con discapacidad motriz. Sugerimos seguir examinando las barreras de ambos profesionales, sus concepciones de EF y el papel del fisioterapeuta, así como su disposición a colaborar entre ellos. O objetivo do estudo foi determinar e analisar as percepções dos professores que lecionam Educação Física (EF) a alunos com deficiência nas classes inclusivas de EF e como melhorar essa inclusão ao colaborar com o fisioterapeuta. A informação foi obtida através de um questionário administrado a 56 professores, que foi completado com entrevistas com nove deles e quatro fisioterapeutas. Os resultados mostram a preocupação e a incerteza dos professores sobre como servir os alunos com deficiência, a fim de oferecer uma educação de qualidade inclusiva e treinamento de demanda e suporte específico em suas aulas. Professores consideram necessário receber apoio de profissionais, como o fisioterapeuta, para que possam ajudar os alunos com deficiência motora. Sugerimos continuar a examinar as barreiras de ambos os profissionais, suas concepções de professores de EF e o papel do fisioterapeuta, bem como a sua vontade de colaborar entre si.

Complete sequence of the genome of the human isolate of Andes virus CHI-7913: comparative sequence and protein structure analysis

Indexación: ScieloWe report here the complete genomic sequence of the Chilean human isolate of Andes virus CHI-7913. The S, M, and L genome segment sequences of this isolate are 1,802, 3,641 and 6,466 bases in length, with an overall GC content of 38.7%. These genome segments code for a nucleocapsid protein of 428 amino acids, a glycoprotein precursor protein of 1,138 amino acids and a RNA-dependent RNA polymerase of 2,152 amino acids. In addition, the genome also has other ORFs coding for putative proteins of 34 to 103 amino acids. The encoded proteins have greater than 98% overall similarity with the proteins of Andes virus isolates AH-1 and Chile R123. Among other sequenced Hantavirus, CHI-7913 is more closely related to Sin Nombre virus, with an overall protein similarity of 92%. The characteristics of the encoded proteins of this isolate, such as hydrophobic domains, glycosylation sites, and conserved amino acid motifs shared with other Hantavirus and other members of the Bunyaviridae family, are identified and discussed

Modified Intracellular-Associated Phenotypes in a Recombinant Salmonella Typhi Expressing S. Typhimurium SPI-3 Sequences

A bioinformatics comparison of Salmonella Pathogenicity Island 3 sequences from S. Typhi and S. Typhimurium serovars showed that ten genes are highly conserved. However three of them are pseudogenes in S. Typhi. Our aim was to understand what functions are lost in S. Typhi due to pseudogenes by constructing a S. Typhi genetic hybrid carrying the SPI-3 region of S. Typhimurium instead of its own SPI-3. We observed that under stressful conditions the hybrid strain showed a clear impairment in resistance to hydrogen peroxide and decreased survival within U937 culture monocytes. We hypothesized that the marT-fidL operon, encoded in SPI-3, was responsible for the new phenotypes because marT is a pseudogen in S. Typhi and has a demonstrated role as a transcriptional regulator in S. Typhimurium. Therefore we cloned and transferred the S. Typhimurium marT-fidL operon into S. Typhi and confirmed that invasion of monocytes was dramatically decreased. Finally, our findings suggest that the genomic and functional differences between SPI-3 sequences have implications in the host specificity of Typhi and Typhimurium serovars

Repair algorithms and penalty functions to handling constraints in an evolutionary scheduling

Ecosystems are directly and negatively affected by many industrial risky activities such as the oil transportation and exploitation. It is therefore important that oil companies carry out a correct maintenance of their oil fields. In this work we implement in our previously proposed evolutionary optimization tool (called PAE) a set of constraint-handling techniques to solve a constrained version of the maintenance problem. The results and comparisons demonstrated that the proposed repair algorithm required less computational effort (evaluations) with the same quality of solutions for the set of instances used.Presentado en XI Workshop Agentes y Sistemas Inteligentes (WASI)Red de Universidades con Carreras en Informática (RedUNCI

Hibridización con búsqueda local de un algoritmo de estimación de distribución para la resolución del problema de secuenciamiento de Flow Shop

Los algoritmos de Estimación de Distribuciones, conocidos como EDAs (Estimation of Distribution Algorithms) son una clase de algoritmos basados en el paradigma de Computación Evolutiva. Los EDAs, sustituyen los mecanismos de variación (cruce y mutación) utilizados tradicionalmente por los Algoritmos Evolutivos (AEs) por la generación de individuos obtenidos por simulación de una distribución de probabilidad. La distribución es estimada a partir del proceso iterativo de competencia de los individuos seleccionados en la generación anterior. Por otra parte, el problema de secuenciamiento de Flow Shop y conocido como FSSP (Flow Shop Secuencing Problem) ha convocado la atención de muchos investigadores en los últimos años. En el caso de makespan, se trata de minimizar el tiempo de salida de la última tarea en la última máquina. Para máquinas mayores e iguales a tres el problema se transforma en NP-hard, conforme se incrementa el número de tareas. Este trabajo, propone aplicar a la resolución del FSSP dos versiones de EDAs, para ser comparados con dos hibridaciones de EDA aplicándole métodos de búsqueda local. Detalles de los algoritmos, experimentos y resultados son presentados en las secciones subsiguientes.Presentado en el X Workshop Agentes y Sistemas InteligentesRed de Universidades con Carreras en Informática (RedUNCI

Hibridización con búsqueda local de un algoritmo de estimación de distribución para la resolución del problema de secuenciamiento de Flow Shop

Los algoritmos de Estimación de Distribuciones, conocidos como EDAs (Estimation of Distribution Algorithms) son una clase de algoritmos basados en el paradigma de Computación Evolutiva. Los EDAs, sustituyen los mecanismos de variación (cruce y mutación) utilizados tradicionalmente por los Algoritmos Evolutivos (AEs) por la generación de individuos obtenidos por simulación de una distribución de probabilidad. La distribución es estimada a partir del proceso iterativo de competencia de los individuos seleccionados en la generación anterior. Por otra parte, el problema de secuenciamiento de Flow Shop y conocido como FSSP (Flow Shop Secuencing Problem) ha convocado la atención de muchos investigadores en los últimos años. En el caso de makespan, se trata de minimizar el tiempo de salida de la última tarea en la última máquina. Para máquinas mayores e iguales a tres el problema se transforma en NP-hard, conforme se incrementa el número de tareas. Este trabajo, propone aplicar a la resolución del FSSP dos versiones de EDAs, para ser comparados con dos hibridaciones de EDA aplicándole métodos de búsqueda local. Detalles de los algoritmos, experimentos y resultados son presentados en las secciones subsiguientes.Presentado en el X Workshop Agentes y Sistemas InteligentesRed de Universidades con Carreras en Informática (RedUNCI

Correlatos de Educación Física medidos objetivamentes en adolescentes con síndrome de Down : Estudio UP & DOWN

Introduction: correlates of physical activity (PA) have not been explored in adolescents with Down syndrome (DS). Understanding correlates of PA could provide information to develop strategies to increase levels of PA in this target population. Objective: the aim of this study was to identify potential correlates of PA in adolescents with DS. Method: information about levels of PA and their potential correlates was collected in 98 adolescents with DS (63 males, aged 11-20 years) using accelerometers and proxy-reported questionnaires. Analysis of covariance and multiple linear regression analyses were conducted to examine correlates of PA. Result: our findings showed that participant’s age and socioeconomic status were associated with levels of PA as non-modifiable correlates. Also, parental support, father PA, television-viewing time with siblings and with friends were associated with levels of PA as modifiable correlates. Discussion and conclusion: both modifiable and non-modifiable factors are associated with levels of PA in adolescents with DS. Therefore, a better understanding of correlates of PA could contribute to develop strategies on PA promotion in adolescents with DS.Introducción: los correlatos de actividad física (AF) no han sido estudiados en adolescentes con síndrome de Down (SD). Entendiendo los correlatos de AF se podría aportar información para desarrollar estrategias para incrementar los niveles de AF en esta población diana. Objetivo: el objetivo de este estudio fue identificar correlatos de AF en adolescentes con SD. Métodos: la información de los niveles de AF y sus potenciales correlatos fue recogida en 98 adolescentes con SD (63 hombres, con edades comprendidas entre 11-20 años), usando acelerómetros y cuestionarios proxy-reportados. Se utilizó análisis de covarianza y análisis de regresión lineal múltiple para examinar los correlatos de AF. Resultados: nuestros resultados muestran que la edad y el estatus socioeconómico de los participantes fue asociado con niveles de AF como correlatos no modificables. Además, el apoyo de los padres, la AF del padre y el tiempo dedicado a ver la televisión con hermanos y amigos fueron asociados con niveles de AF como correlatos modificables. Discusión y conclusión: ambos factores modificables y no modificables se asocian con niveles de AF en adolescentes con SD. Por lo tanto, una mejor comprensión de los correlatos de AF podría contribuir a desarrollar estrategias de promoción de la AF en adolescentes con SD