Uma Leitura de A Origem da Obra de Arte de Heidegger

Tomando como referência o texto A Origem da Obra de Arte de Heidegger, o objectivo desta dissertação é a análise da questão da obra de arte como verdade poética. Considerando a experiência do conhecimento como a origem da criação, por via do que o mundo revela, e considerando tanto a produção artística como outras formas de produção pela mão do homem enquanto atitudes de apresentação da verdade encontrada, ela reflecte acerca daquilo que a obra de arte tem e que a distingue das demais criações do home

Contributos para a adaptação da entrevista clínica geracional à população portuguesa

O presente estudo teve como objetivo contribuir para a adaptação e validação da Intervista Clinica Generazionale (ICG) de Cigoli e Tamanza (2009), anteriormente submetida a um processo de adaptação transcultural para a língua portuguesa por Raguso, Facchin, Molgora e Gonçalves (2010) e intitulada como Entrevista Clínica Geracional (ECG). A ECG é um instrumento clínico para a avaliação e investigação das relações familiares centrado em particular no conceito de Generatividade das famílias. A ECG é composta por diferentes dimensões a saber pelo Eixo I - As Origens, pelo Eixo II - O Casal e o pelo Eixo III - Passagem Generativa. Foi no presente estudo aplicada a 10 casais portugueses visando o teste das propriedades de medida, especificamente a validade de constructo, através do uso do teste da validade convergente (Freire & Almeida, 2001). Para estudar a validade convergente utilizou-se a análise de comparação usando o teste de Fisher para testar a associação entre os Eixos da ECG (Eixo I - As Origens, Eixo II - O Casal e o Eixo III - Passagem Generativa) com outros instrumentos: o Family Environment Scale (FES) e o Family Adaptability and Cohesion Evaluation Scales (FACES IV); a Escala de Avaliação da Satisfação em Áreas da Vida Conjugal (EASAVIC); e, por último, a Loyola Generativity Scale (LGS), respetivamente; que avaliam constructos teoricamente relacionados (e.g., coesão familiar, expressividade, comunicação, etc) e calculou-se, subsequentemente, as suas correlações (coeficiente de correlação não-paramétrica de Spearman). As análises de correlação entre Eixos I (Q1, Q6, Q7), II (Q5,6,8.1/8.2), III (Q3.1/3.2) da ECG com os scores das escalas/subescalas da FES (‘Ambiente familiar’, ‘Expressividade’) e FACES IV (‘Coesão equilibrada’, ‘Flexibilidade’), EASAVIC (‘Próprio’, ‘outro’, total’) e LGS mostraram, respetivamente, no mesmo sentido da análise de Fisher, que existem coeficientes de correlação positiva estatisticamente significativos, indicando validade convergente para as respetivas dimensões. De salientar que a validade convergente entre Eixo I (Q1) e a subescalas da FES (‘Ambiente familiar’) apenas foram verificadas para os cônjuges do género feminino, assim como entre o Eixos I (Q7) e a subescalas da FES (‘Expressividade’), assim como, entre Eixo I (Q6,7) e a subescalas da FACES IV (‘Coesão equilibrada’, ‘Flexibilidade’) apenas ocorreram para os cônjuges do género masculino. Em suma, os presentes resultados mostram que a ECG, na sua versão portuguesa, possui boa qualidade psicométrica e validade, e aponta para a sua utilidade na avaliação da dinâmica familiar para as respetivas dimensões onde foram encontradas validade convergente.This research project aims to contribute to the adaptation and validation of the Interview Clinic Generazionale (ICG) of Cigoli and Tamanza (2009), previously subjected to a process of cultural adaptation to Portuguese by Raguso, Facchin, Molgora and Gonçalves (2010), entitled ‘The Clinical Generational Interview’ (CGI). The CGI is a clinical tool for the evaluation and research of family relationships focused in particular on the concept of Family Generativity. The CGI is composed by different dimensions namely the Axis I - The family of origin, the Axis II - The couple and the Axis III - The generational passage. In this study the CGI was applied to 10 Portuguese couples in order to analyze their measurement properties , in particular the construct validity through the use of convergent validity procedures (Freire & Almeida,2001). To study the convergent validity was used the comparison analysis using the Fisher's exact test and the coefficient of non-parametric Spearman correlation to explore the association/correlation between the axes of the CGI (Axis I - The family of origin, Axis II - The couple and Axis III - The generational passage) with other instruments, such as: the Family Environment Scale (FES) and the Family Adaptability and Cohesion Evaluation Scales (FACES IV); the Scale of Assessment of Satisfaction in Areas of Conjugal Life (EASAVIC) and, lastly, the Generativity Loyola Scale (LGS), respectively; since these instruments assess theoretical constructs related to those covered by the CGI (e.g., family cohesion, expressiveness, communication, etc.). The correlation analysis between Axis I (Q1, Q6, Q7), II (Q5,6,8.1 / 8.2), III (Q3.1 / 3.2) of the CGI with the scores of the subscales of FES ( 'Family environment’ and 'Expressive') and of FACES IV ( 'Balanced Cohesion' and 'Flexibility'), EASAVIC ( 'Self', 'Other' and total scores ') and LGS showed, respectively, in the same sense of Fisher's analysis, statistically significant positive relationships, indicating convergent validity for the respective dimensions. To point out that the convergent validity between Axis I (Q1) and the subscales of the FES ('Family environment’') were only founded for the female gender spouses group, as well as the association between the Axis I (Q7) and the subscales of the FES ( 'expressiveness ') and the Axis I (Q6,7) and subscales faces IR (' Balanced cohesion, '' Flexibility ') only was observed for the males spouses group. In short, the present results show that the CGI in its Portuguese version, has good psychometric quality and validity, and points to its usefulness in the assessment of family dynamics to the respective dimensions which were found convergent validity

O papel dos cuidados continuados na reconstrução da autonomia

info:eu-repo/semantics/publishedVersio

Quanto tempo dura um instante?: da dualidade do efémero e da permanência do serviço educativo

Relatório de Estágio submetido à Escola Superior de Teatro e Cinema para cumprimento dos requisitos necessários à obtenção do grau de Mestre em Teatro – Especialização em ProduçãoOs serviços educativos em Portugal começam a ganhar notoriedade dentro do funcionamento das entidades culturais. São encarados como um serviço promissor que possibilita a variedade de programações e, por isso, uma captação e fidelização de públicos mais vasta. É com a percepção do fenómeno dos serviços educativos e da importância que lhes deve ser prestada no sentido de se projectar e influenciar as práticas culturais que surge o objecto de estudo deste relatório. O capítulo I é dedicado à teorização do conceito de serviço educativo, no que diz respeito aos seus objectivos, missões e conceitos que o sustentam. O capítulo II é apresentado como um relatório de estágio, desenvolvido no CCB Fábrica das Artes com a duração de seis meses, de Abril a Outubro de 2010, sendo descritas determinadas actividades programadas, as funções e as aquisições.ABSTRACT - Educational services in Portugal are beginning to gain notoriety within the action of cultural entities. They are seen as a promising service that enables the variety of programming and therefore, a wider capture and retention of public. It is with the perception of the phenomenon of educational services and the importance that they should be given, in order to project and induce cultural practices, that arises the object of study in this report. Chapter I is devoted to theorizing the concept of educational services, concerning its objectives, tasks and concepts that support it. The chapter II is presented as a probation report, developed at CCB – Arts Factory, with a duration of six months, from April to October 2010, describing the share of scheduled activities, functions and acquisitions

The defensive functions of plant inhibitors are not restricted to insect enzyme inhibition

Three plant proteinase inhibitors BbKI (kallikrein inhibitor) and BbCI (cruzipain inhibitor) from Bauhinia bouhinioides, and a BrTI (trypsin inhibitor) from B. rufa, were examined for other effects in Callosobruchus maculatus development; of these only BrTI affected bruchid emergence. BrTI and BbKI share 81% identities in their primary sequences and the major differences between them are the regions comprising the RGD and RGE motifs in BrTI. These sequences were shown to be essential for BrTI insecticidal activity, since a modified BbKI [that is a recombinant form (BbKIm) with some amino acid residues replaced by those found in BrTI sequence] also strongly inhibited insect development. By using synthetic peptides related to the BrTI sequence, YLEAPVARGDGGLA-NH(2) (RGE) and IVYYPDRGETGL-NH(2) (RGE), it was found that the peptide with an RGE sequence was able to block normal development of C. maculatus larvae (ED(50) 0.16% and LD(50) 0.09%), this being even more effective than the native protein. (C) 2009 Elsevier B.V. All rights reserved.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAP/FADA (UNIFESP)Universidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04044020 São Paulo, BrazilUniv Fed Mato Grosso, Dept Nat Sci, BR-79603011 Tres Lagoas, MS, BrazilUniv Estadual Fluminense Darcy Ribeiro, Lab Prot & Peptide Biochem, CBB, BR-28015620 Campos Dos Goytacazes, RJ, BrazilUniversidade Federal de São Paulo, Dept Biophys, BR-04044020 São Paulo, BrazilUniv São Paulo, Inst Fis Sao Carlos, Sao Carlos, SP, BrazilUniv Estadual Oeste Parana, Ctr Engn & Ciencias Exatas, BR-85903000 Toledo, PR, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, BR-04044020 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biophys, BR-04044020 São Paulo, BrazilWeb of Scienc

Exuberant Hand-Foot-Mouth Disease: An Immunocompetent Adult with Atypical Findings

Non-polio enteroviruses are ubiquitous viruses responsible for a wide spectrum of disease in people of all ages, although infection and illness disproportionately affect infants and young children. Hand-foot-mouth disease (HFMD) is an enteroviral clinical syndrome most frequently caused by coxsackievirus-A16 and enterovirus-A71. Since 2008, a novel coxsackievirus-A6 genotype has been associated with more severe HFMD in both children and adults, presenting with a unique constellation of findings, and whose prevalence has been increasing over the last few years. In this case report, an atypical clinical picture of confirmed enterovirus HFMD is described in an immunocompetent adult, with exuberant clinical findings, clinically consistent with coxsackievirus-A6 infection. This case report highlights the importance of awareness of the clinical presentation of this increasingly common infection in adult

Antimicrobial effect of essential oils of Laurus nobilis L. and Rosmarinus officinallis L. on shelf-life of minced “Maronesa¿? beef stored under different packaging conditions

The aim of this study was to evaluate the effect of essential oils (EOs) of plants naturally occurring in northern Portugal on the spoilage of fresh Maronesa beef burgers stored at 2 and 8 C under different packaging conditions. EOs were obtained from dried leaves of laurel(Laurus Nobilis L.) and rosemary (Rosmarinus officinallis L.) by hydro-distillation using a Clevenger-type apparatus. Analysis of volatile composition of essential oils of rosemary and laurel was achieved by Gas Chromatography–Mass Spectrometry (GC–MS) and Gas Chromatography-Thermal Conductivity Detection (GC-TCD) resulting in the detection of 95.8% and 89.4% of its compounds, respectively. Fresh beef (semitendinosus and semimembranosus) of DOP-Maronesa breed (males; n = 4) were obtained from local market and transported to the laboratory. Samples were stored at 2 and 8 C in two different conditions: aerobiosis (A) and vacuum (V) and analyzed at 0, 1, 2, 3, 5, 7, 10, 14, 21 and 28 days for Lactic acid bacteria (LAB), Enterobacteriaceae,Pseudomonas spp., Fungi, Total mesophilic (TM) and psychrotrophic (TP), color (L*a*b*) and pH. Laurel was the most effective EO keeping pH from increasing. Coordinates L* and a* were higher on samples containing laurel EO for both A and V packaging. Laurel also showed better effect in reducing microbiologic counts in samples packed in A at both 2 and 8 C and packed in V at 8 C. Rosemary was effective in reducing microbial counts on all V samples stored at 2 C. This study allows to conclude that Laurel EO has significant effect in shelf-life, maintaining fresh beef colo

Purification and characterization of a new trypsin inhibitor from Dimorphandra mollis seeds

A second trypsin inhibitor (DMTI-II) was purified from the seed of Dimorphandra mollis (Leguminosae-Mimosoideae) by ammonium sulfate precipitation (30-60%), gel filtration, and ion-exchange and affinity chromatography. A molecular weight of 23 kDa was estimated by gel filtration on a Superdex 75 column SDS-PAGE under reduced conditions showed that DMTI-II consisted of a single polypeptide chain, although isoelectric focusing revealed the presence of three isoforms. the dissociation constant of 1.7 x 10(-9) M with bovine trypsin indicated a high affinity between the inhibitor and this enzyme. the inhibitory activity was stable over a wide pH range and in the presence of DTT. the N-terminal sequence of DMTI-II showed a high degree of homology with other Kunitz-type inhibitors.Univ Fed Mato Grosso do Sul, Dept Ciencias Nat Tres Lagoas, CEUL, BR-79603011 Tres Lagoas, MS, BrazilUniv Estadual Campinas, Dept Bioquim, Inst Biol, Campinas, SP, BrazilEscola Paulista Med, Dept Bioquim, BR-04023 São Paulo, BrazilUniv Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Campo Dos Goytacazes, RJ, BrazilEscola Paulista Med, Dept Bioquim, BR-04023 São Paulo, BrazilWeb of Scienc

Enterolobium contortisiliquum Trypsin Inhibitor (EcTI), a Plant Proteinase Inhibitor, Decreases in Vitro Cell Adhesion and Invasion by Inhibition of Src Protein-Focal Adhesion Kinase (FAK) Signaling Pathways

Tumor cell invasion is vital for cancer progression and metastasis. Adhesion, migration, and degradation of the extracellular matrix are important events involved in the establishment of cancer cells at a new site, and therefore molecular targets are sought to inhibit such processes. the effect of a plant proteinase inhibitor, Enterolobium contortisiliquum trypsin inhibitor (EcTI), on the adhesion, migration, and invasion of gastric cancer cells was the focus of this study. EcTI showed no effect on the proliferation of gastric cancer cells or fibroblasts but inhibited the adhesion, migration, and cell invasion of gastric cancer cells; however, EcTI had no effect upon the adhesion of fibroblasts. EcTI was shown to decrease the expression and disrupt the cellular organization of molecules involved in the formation and maturation of invadopodia, such as integrin beta 1, cortactin, neuronal Wiskott-Aldrich syndrome protein, membrane type 1 metalloprotease, and metalloproteinase-2. Moreover, gastric cancer cells treated with EcTI presented a significant decrease in intracellular phosphorylated Src and focal adhesion kinase, integrin-dependent cell signaling components. Together, these results indicate that EcTI inhibits the invasion of gastric cancer cells through alterations in integrin-dependent cell signaling pathways.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de São Paulo, Escola Paulista Med, Dept Biochem & Mol Biol, BR-04044020 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04044020 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biochem & Mol Biol, BR-04044020 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04044020 São Paulo, BrazilFAPESP: 2009/53766-5Web of Scienc