Laboratory study of the impact of repetitive electrical and mechanical stimulation on brown shrimp Crangon crangon

Pulse trawling is currently the best available alternative to beam trawling in the brown shrimp Crangon crangon and Sole Solea solea (also known as Solea vulgaris) fisheries. To evaluate the effect of repetitive exposure to electrical fields, brown shrimp were exposed to the commercial electrodes and pulse settings used to catch brown shrimp (shrimp startle pulse) or Sole (Sole cramp pulse) 20 times in 4 d and monitored for up to 14 d after the first exposure. Survival, egg loss, molting, and the degree of intranuclear bacilliform virus (IBV) infection were evaluated and compared with those in stressed but not electrically exposed (procedural control) and nonstressed, nonexposed (control) brown shrimp as well as brown shrimp exposed to mechanical stimuli. The lowest survival at 14 d (57.3%) occurred in the Sole cramp pulse treatment, and this was significantly lower than in the group with the highest survival, the procedural control (70.3%). No effect of electrical stimulation on the severity of IBV infection was found. The lowest percentage of molts occurred in the repetitive mechanical stimulation treatment (14.0%), and this was significantly lower than in the group with the highest percentage of molts, the procedural control (21.7%). Additionally, the mechanically stimulated brown shrimp that died during the experiment had a significantly larger size than the surviving individuals. Finally, no effect of the shrimp startle pulse was found. Therefore, it can be concluded that repetitive exposure to a cramp stimulus and mechanical stimulation may have negative effects on the growth and/or survival of brown shrimp. However, there is no evidence that electrical stimulation during electrotrawls would have a larger negative impact on brown shrimp stocks than mechanical stimulation during conventional beam trawling

Repositioning the Catalytic Triad Aspartic Acid of Haloalkane Dehalogenase: Effects on Stability, Kinetics, and Structure

Haloalkane dehalogenase (DhlA) catalyzes the hydrolysis of haloalkanes via an alkyl-enzyme intermediate. The covalent intermediate, which is formed by nucleophilic substitution with Asp124, is hydrolyzed by a water molecule that is activated by His289. The role of Asp260, which is the third member of the catalytic triad, was studied by site-directed mutagenesis. Mutation of Asp260 to asparagine resulted in a catalytically inactive D260N mutant, which demonstrates that the triad acid Asp260 is essential for dehalogenase activity. Furthermore, Asp260 has an important structural role, since the D260N enzyme accumulated mainly in inclusion bodies during expression, and neither substrate nor product could bind in the active-site cavity. Activity for brominated substrates was restored to D260N by replacing Asn148 with an aspartic or glutamic acid. Both double mutants D260N+N148D and D260N+N148E had a 10-fold reduced kcat and 40-fold higher Km values for 1,2-dibromoethane compared to the wild-type enzyme. Pre-steady-state kinetic analysis of the D260N+N148E double mutant showed that the decrease in kcat was mainly caused by a 220-fold reduction of the rate of carbon-bromine bond cleavage and a 10-fold decrease in the rate of hydrolysis of the alkyl-enzyme intermediate. On the other hand, bromide was released 12-fold faster and via a different pathway than in the wild-type enzyme. Molecular modeling of the mutant showed that Glu148 indeed could take over the interaction with His289 and that there was a change in charge distribution in the tunnel region that connects the active site with the solvent. On the basis of primary structure similarity between DhlA and other α/β-hydrolase fold dehalogenases, we propose that a conserved acidic residue at the equivalent position of Asn148 in DhlA is the third catalytic triad residue in the latter enzymes.

Variations in Stellar Clustering with Environment: Dispersed Star Formation and the Origin of Faint Fuzzies

The observed increase in star formation efficiency with average cloud density, from several percent in whole giant molecular clouds to ~30 or more in cluster-forming cores, can be understood as the result of hierarchical cloud structure if there is a characteristic density as which individual stars become well defined. Also in this case, the efficiency of star formation increases with the dispersion of the density probability distribution function (pdf). Models with log-normal pdf's illustrate these effects. The difference between star formation in bound clusters and star formation in loose groupings is attributed to a difference in cloud pressure, with higher pressures forming more tightly bound clusters. This correlation accounts for the observed increase in clustering fraction with star formation rate and with the observation of Scaled OB Associations in low pressure environments. ``Faint fuzzie'' star clusters, which are bound but have low densities, can form in regions with high Mach numbers and low background tidal forces. The proposal by Burkert, Brodie & Larsen (2005) that faint fuzzies form at large radii in galactic collisional rings, satisfies these constraints.Comment: 14 pages, 2 figures, ApJ, 672, January 10th 200

Dynamic Neuromuscular Control of the Lower Limbs in Response to Unexpected Single-Planar versus Multi-Planar Support Perturbations in Young, Active Adults.

PURPOSE: An anterior cruciate ligament (ACL) injury involves a multi-planar injury mechanism. Nevertheless, unexpected multi-planar perturbations have not been used to screen athletes in the context of ACL injury prevention yet could reveal those more at risk. The objective of this study was to compare neuromuscular responses to multi-planar (MPP) and single-planar perturbations (SPP) during a stepping-down task. These results might serve as a basis for future implementation of external perturbations in ACL injury screening programs. METHODS: Thirteen young adults performed a single leg stepping-down task in eight conditions (four MPP and four SPP with a specified amplitude and velocity). The amplitudes of vastus lateralis (VL), vastus medialis (VM), hamstrings lateralis (HL), hamstrings medialis (HM) EMG activity, medio-lateral and anterior-posterior centre of mass (COM) displacements, the peak knee flexion and abduction angles were compared between conditions using an one-way ANOVA. Number of stepping responses were monitored during all conditions. RESULTS: Significantly greater muscle activity levels were found in response to the more challenging MPP and SPP compared to the less challenging conditions (p < 0.05). No differences in neuromuscular activity were found between the MPP conditions and their equivalents in the SPP. Eighteen stepping responses were monitored in the SPP versus nine in the MPP indicating that the overall neuromuscular control was even more challenged during the SPP which was supported by greater COM displacements in the SPP. CONCLUSION: The more intense MPP and SPP evoked different neuromuscular responses resulting in greater muscle activity levels compared to small perturbations. Based on the results of COM displacements and based on the amount of stepping responses, dynamic neuromuscular control of the knee joint appeared less challenged during the MPP. Therefore, future work should investigate extensively if other neuromuscular differences (i.e. co-activation patterns and kinetics) exist between MPP and SPP. In addition, future work should examine the influence on the neuromuscular control of the magnitude of the perturbations and the magnitude of stepping height and stepping distance

Social Actors “to Go”:An Analytical Toolkit to Explore Agency in Business Discourse and Communication

We argue that language awareness and discourse analytical skills should be part of business communication curricula. To this end, we propose a three-step analytical model drawing on organizational and critical discourse studies, and approaches from systemic-functional linguistics, to explore agency and action in business communication. Focusing on language and discourse helps students to analyze texts more systematically, researchers to gain deeper insights into organizational discourse, and practitioners to reflect on communication processes and produce texts with more impact. We view discourse as central to organizational processes and render a specific approach accessible and easy to integrate into business communication curricula

The effect of the dynamical state of clusters on gas expulsion and infant mortality

The star formation efficiency (SFE) of a star cluster is thought to be the critical factor in determining if the cluster can survive for a significant (>50 Myr) time. There is an often quoted critical SFE of ~30 per cent for a cluster to survive gas expulsion. I reiterate that the SFE is not the critical factor, rather it is the dynamical state of the stars (as measured by their virial ratio) immediately before gas expulsion that is the critical factor. If the stars in a star cluster are born in an even slightly cold dynamical state then the survivability of a cluster can be greatly increased.Comment: 6 pages, 2 figures. Review talk given at the meeting on "Young massive star clusters - Initial conditions and environments", E. Perez, R. de Grijs, R. M. Gonzalez Delgado, eds., Granada (Spain), September 2007, Springer: Dordrecht. Replacement to correct mistake in a referenc

On the Rapid Collapse and Evolution of Molecular Clouds

Stars generally form faster than the ambipolar diffusion time, suggesting that several processes short circuit the delay and promote a rapid collapse. These processes are considered here, including turbulence compression in the outer parts of giant molecular cloud (GMC) cores and GMC envelopes, GMC core formation in an initially supercritical state, and compression-induced triggering in dispersing GMC envelopes. The classical issues related to star formation timescales are addressed: high molecular fractions, low efficiencies, long consumption times for CO and HCN, rapid GMC core disruption and the lack of a stable core, long absolute but short relative timescales with accelerated star formation, and the slow motions of protostars. We consider stimuli to collapse from changes in the density dependence of the ionization fraction, the cosmic ray ionization rate, and various dust properties at densities above ~10^5 cm^{-3}. We favor the standard model of subcritical GMC envelops and suggest they would be long lived if not for disruption by rapid star formation in GMC cores. The lifecycle of GMCs is illustrated by a spiral arm section in the Hubble Heritage image of M51, showing GMC formation, star formation, GMC disruption with lingering triggered star formation, and envelope dispersal. There is no delay between spiral arm dustlanes and star formation; the classical notion results from heavy extinction in the dust lane and triggered star formation during cloud dispersal. Differences in the IMF for the different modes of star formation are considered.Comment: 46 pages, 5 figures, scheduled for ApJ 668, October 20, 200