Marine Low Clouds : Radiation, Turbulence, and Forecasting

High-resolution ship-based observations of a nearly uniform stratocumulus deck in the southeast Pacific during 17 days of the 2008 VOCALS regional experiment elucidate radiation and turbulence in the marine boundary layer (MBL). A new method for prescribing observations-based cloud properties to the Rapid Radiative Transfer Model is presented and applied to the VOCALS dataset. The simulated radiative fluxes compare well with surface observations and with simulations from several other commonly employed methods, particularly during times of high drizzle content and/or precipitation. Clear-sky and cloudy scenarios illustrate the radiative effect of clouds on the MBL. Turbulence estimates are made for the stratocumulus layer using the simulated radiative fluxes and vertical velocity observations from the NOAA W-band Doppler cloud radar. The averages and diurnal cycles of the terms in the turbulent kinetic energy (TKE) budget are presented. 10-minute vertical velocity statistics and estimates of TKE budget terms reveal a robust diurnal cycle of weak turbulence during daylight hours with a rapid increase near sundown followed by sustained strong turbulence at night. Buoyant production is driven by cloud-top longwave radiative cooling during the night, but is offset and dominated by absorption of solar radiation from ~0900-1600 LT. At night, dissipation increases toward cloud base while vertical velocity variance is nearly constant with height. This suggests larger-scale turbulence occurs near cloud-top while dissipation interacts with smaller-scale turbulence near cloud base. Cloud-top entrainment rates are estimated from the entrainment heat flux calculated as a residual from the heat budget. Entrainment, with an average value of 4.6 mm s-1, has a diurnal cycle similar to turbulence with larger values at night. The third part of this dissertation presents one of the first studies using reforecast data to produce downscaled low cloud probabilistic forecasts for aviation applications. Probability of low cloud ceiling and visibility are forecast using analogs chosen from an ensemble of reforecasts, on the basis of their agreement with thermodynamic soundings. Skillful forecasts are produced for up to 30-hour leads times using reforecasts of 1° resolution at major airports across the continental United States. These probabilistic forecasts outperform climatology at all lead times and trained forecasters at nine-hour and longer lead times. These results suggest that real-time forecasts based on analog reforecasts is a useful approach for aviation applications with potential to enhance safety and commerce

The Time Scales of Variability of Marine Low Clouds

Permission to use figures, tables, and brief excerpts from this work in scientific and educational works is hereby granted provided that the source is acknowledged. Any use of material in this work that is determined to be “fair use” under Section 107 of the U.S. Copyright Act or that satisfies the conditions specified in Section 108 of the U.S. Copyright Act (17 USC §108) does not require the AMS’s permission. Republication, systematic reproduction, posting in electronic form, such as on a website or in a searchable database, or other uses of this material, except as exempted by the above statement, requires written permission or a license from the AMS. All AMS journals and monograph publications are registered with the Copyright Clearance Center (http://www.copyright.com). Questions about permission to use materials for which AMS holds the copyright can also be directed to the AMS Permissions Officer at [email protected]. Additional details are provided in the AMS Copyright Policy statement, available on the AMS website (http://www.ametsoc.org/CopyrightInformation).Multidecade global regressions of inversion strength, vertical velocity, and sea surface temperature (SST) on low cloud amount, from subdaily to multiyear time scales, refute the dominance of seasonal inversion strength on marine low cloud variability. Multiday low cloud variance averaged over the eastern Pacific and Atlantic stratocumulus regions [5 × 10−2 (cloud amount)2] is twice the subdaily variance and 5 times larger than the multimonth variance. The broad multiday band contains most (60%) of the variance, despite strong seasonal (annual) and diurnal spectral peaks. Multiday low cloud amount over the eastern tropical and midlatitude oceans is positively correlated to inversion strength, with a slope of 2%–5% K−1. Anecdotes show multiday low cloud and inversion strength anomalies propagate equatorward from midlatitudes. Previously shown correlations of low clouds to strong inversions and cool SST on monthly and longer time scales in the stratocumulus regions imply positive cloud-radiative feedbacks, with e-folding time scales of 300 days for SST and 14 days for atmospheric boundary layer temperature. On multimonth time scales, removing the effect of SST on low clouds reduces the low cloud amount explained by inversion strength by a factor of 3, but SST has a small effect at other time scales. Contrary to their positive correlation in the stratocumulus cloud decks, low clouds are anticorrelated to inversion strength over most of the tropics on daily and subdaily time scales

Field validation of radar systems for monitoring bird migration

This is the author accepted manuscript. The final version is available from Wiley via the DOI in this record.Advances in information technology are increasing the use of radar as a tool to investigate and monitor bird migration movements. We set up a field campaign to compare and validate outputs from different radar systems. Here we compare the pattern of nocturnal bird migration movements recorded by four different radar systems at a site in southern Sweden. Within the range of the weather radar (WR) Ängelholm, we operated a "BirdScan" (BS) dedicated bird radar, a standard marine radar (MR), and a tracking radar (TR). The measures of nightly migration intensities, provided by three of the radars (WR, BS, MR), corresponded well with respect to the relative seasonal course of migration, while absolute migration intensity agreed reasonably only between WR and BS. Flight directions derived from WR, BS and TR corresponded very well, despite very different sample sizes. Estimated mean ground speeds differed among all four systems. The correspondence among systems was highest under clear sky conditions and at high altitudes. Synthesis and applications. While different radar systems can provide useful information on nocturnal bird migration, they have distinct strengths and weaknesses, and all require supporting data to allow for species level inference. Weather radars continuously detect avian biomass flows across a wide altitude band, making them a useful tool for monitoring and predictive applications at regional to continental scales that do not rely on resolving individuals. BirdScan and marine radar's strengths are in local and low altitude applications, such as collision risks with man-made structures and airport safety, although marine radars should not be trusted for absolute intensities of movement. In quantifying flight behaviour of individuals, TR is the most informative.We acknowledge the support provided by COST – European Cooperation in Science and Technology through the Action ES1305 ‘European Network for the Radar Surveillance of Animal Movement’ (ENRAM) in facilitating this collaboration. The study received financial support from Gyllenstierna Krapperup ́s Foundation and the Centre for Animal Movement Research (CAnMove) financed by a Linnaeus grant (349-2007-8690) from the Swedish Research Council and Lund University

Dendritic cell vaccination as postremission treatment to prevent or delay relapse in acute myeloid leukemia

Relapse is a major problem in acute myeloid leukemia (AML) and adversely impacts survival. In this phase II study, we investigated the effect of vaccination with dendritic cells (DCs) electroporated with Wilms’ tumor 1 (WT1) mRNA as post-remission treatment in 30 AML patients at very high risk of relapse. There was a demonstrable anti-leukemic response in 13 patients. Nine patients achieved molecular remission as demonstrated by normalization of WT1 transcript levels, 5 of which are sustained after a median follow-up of 109.4 months. Disease stabilization was achieved in 4 other patients. Five-year overall survival (OS) was higher in responders than in non-responders (53.8% vs. 25.0%; P=0.01). In patients receiving DCs in first complete remission (CR1), there was a vaccine-induced relapse reduction rate of 25% and the 5-year relapse-free survival was higher in responders than in non-responders (50% vs. 7.7%; P65 years who received DCs in CR1, 5-year OS was 69.2% and 30.8% respectively, as compared to 51.7% and 18% in the Swedish Acute Leukemia Registry (SALR). Long-term clinical response was correlated with increased circulating frequencies of poly-epitope WT1-specific CD8+ T-cells. Long-term OS was correlated with interferon-γ+ and tumor necrosis factor-α+ WT1-specific responses in delayed type hypersensitivity-infiltrating CD8+ T-lymphocytes. In conclusion, vaccination of AML patients with WT1 mRNA-electroporated DCs can be an effective strategy to prevent or delay relapse after standard chemotherapy, translating into improved OS rates, which are correlated with the induction of WT1-specific CD8+ T-cell response. This trial was registered at www.clinicaltrials.gov as #NCT00965224

Retinoic acid induces HL-60 cell differentiation via the upregulation of miR-663

<p>Abstract</p> <p>Background</p> <p>Differentiation of the acute myeloid leukemia (AML) cell line HL-60 can be induced by all trans-retinoic acid (ATRA); however, the mechanism regulating this process has not been fully characterized.</p> <p>Methods</p> <p>Using bioinformatics and <it>in vitro </it>experiments, we identified the microRNA gene expression profile of HL-60 cells during ATRA induced granulocytic differentiation.</p> <p>Results</p> <p>Six microRNAs were upregulated by ATRA treatment, miR-663, miR-494, miR-145, miR-22, miR-363* and miR-223; and three microRNAs were downregulated, miR-10a, miR-181 and miR-612. Additionally, miR-663 expression was regulated by ATRA. We used a lentivirus (LV) backbone incorporating the spleen focus forming virus (SFFV-F) promoter to drive miR-663 expression, as the CMV (Cytomegalovirus) promoter is ineffective in some lymphocyte cells. Transfection of LV-miR-663 induced significant HL-60 cell differentiation <it>in vitro</it>.</p> <p>Conclusions</p> <p>Our results show miR-663 may play an important role in ATRA induced HL-60 cell differentiation. Lentivirus delivery of miR-663 could potentially be used directly as an anticancer treatment in hematological malignancies</p

Transesterification of PHA to Oligomers Covalently Bonded with (Bio)Active Compounds Containing Either Carboxyl or Hydroxyl Functionalities

© 2015 The Authors. Published by Public Library of Science. This is an open access article available under a Creative Commons licence. The published version can be accessed at the following link on the publisher’s website: https://doi.org/10.1371/journal.pone.0120149This manuscript presents the synthesis and structural characterisation of novel biodegradable polymeric controlled-release systems of pesticides with potentially higher resistance to weather conditions in comparison to conventional forms of pesticides. Two methods for the preparation of pesticide-oligomer conjugates using the transesterification reaction were developed. The first method of obtaining conjugates, which consist of bioactive compounds with the carboxyl group and polyhydroxyalkanoates (PHAs) oligomers, is "one-pot" transesterification. In the second method, conjugates of bioactive compounds with hydroxyl group and polyhydroxyalkanoates oligomers were obtained in two-step method, through cyclic poly(3-hydroxybutyrate) oligomers. The obtained pesticide-PHA conjugates were comprehensively characterised using GPC, 1H NMR and mass spectrometry techniques. The structural characterisation of the obtained products at the molecular level with the aid of mass spectrometry confirmed that both of the synthetic strategies employed led to the formation of conjugates in which selected pesticides were covalently bonded to PHA oligomers via a hydrolysable ester bond