Experimental Study on a Laboratory Test Bench for Sea Wave Generation Systems

Abstract The paper presents a laboratory test bench specifically designed for sea wave generation systems. In particular a DC Micro Grid is realized to experimentally validate the energy performance of a PM Brushless ball screw actuator, during motor-regenerative operative conditions, which is representative of an oscillating body wave generation system. The proposed architecture is based on a DC bus, which features the integration of renewable energy sources and buffered storage systems, with the aim of smoothing the natural power fluctuations of wave energy generation systems. The wave generation is simulated in laboratory by controlling an electric motor, which is directly coupled with the PM brushless generator. The experimental validation phase is mainly devoted to verify the design criteria of the architecture scheme and the control strategies of the power fluxes related to power converters

Cellular-Automata model for dense-snow avalanches

This paper introduces a three-dimensional model for simulating dense-snow avalanches, based on the numerical method of cellular automata. This method allows one to study the complex behavior of the avalanche by dividing it into small elements, whose interaction is described by simple laws, obtaining a reduction of the computational power needed to perform a three-dimensional simulation. Similar models by several authors have been used to model rock avalanches, mud and lava flows, and debris avalanches. A peculiar aspect of avalanche dynamics, i.e., the mechanisms of erosion of the snowpack and deposition of material from the avalanche is taken into account in the model. The capability of the proposed approach has been illustrated by modeling three documented avalanches that occurred in Susa Valley (Western Italian Alps). Despite the qualitative observations used for calibration, the proposed method is able to reproduce the correct three-dimensional avalanche path, using a digital terrain model, and the order of magnitude of the avalanche deposit volume

NW Adriatic Sea variability in relation to chlorophyll-<I>a</I> dynamics in the last 20 years (1986?2005)

International audienceThis paper presents a long-term time series (1986?2005) of hydrological and biogeochemical data, both published and unpublished. Data were collected in the north-western area of the Adriatic Sea, at two stations that are considered hydrodynamically and trophically different. The time series have been statistically and graphically investigated on a monthly scale in order to find not only possible chlorophyll-a trends over time, but also links between the concentrations of chlorophyll-a and the variability in the environment, as well as trophic differences between the two areas. Basically, in both cases the statistical test results show no significant trends in either the average chlorophyll-a values or in dispersion of the data, in contrast with significant trends in temperature and salinity. The two areas have similar hydrological features, yet they present significant differences in the amount of nutrient inputs: these are in fact higher at the coastal site, which is characterized by a prevalence of surface blooms, while they are lower at the offshore station, which is mainly affected by intermediate blooms. Nonetheless, throughout the whole water column, chlorophyll-a concentrations are only slightly different. Both areas are affected by riverine discharge, though in the first case considered chlorophyll-a concentrations are also driven strongly by the seasonal cycle. Finally, the results show that the two stations are not trophically different, although some controlling factors, such as zooplankton grazing in one case and light attenuation in the other, may regulate the growth of phytoplankton

NW Adriatic Sea biogeochemical variability in the last 20 years (1986–2005)

International audience; This paper presents a long-term time series (1986?2005) of hydrological and biogeochemical data, both published and unpublished. Data were collected in the north-western area of the Adriatic Sea, at two stations that are considered hydrodynamically and trophically different. The time series have been statistically and graphically analysed on a monthly scale in order to study the annual climatologies, links between the concentrations of chlorophyll-a and the variability in the environment, trophic differences between the two areas and chlorophyll-a trends over time. Basically, the two areas have similar hydrological features, yet they present significant differences in the amount of nutrient inputs: these are in fact higher at the coastal site, which is characterized by a prevalence of surface blooms, while they are lower at the offshore station, which is mainly affected by blooms at intermediate depths. Nonetheless, throughout the whole water column, chlorophyll-a concentrations are only slightly different. Both areas are affected by riverine discharge, though chlorophyll-a concentrations are also driven strongly by the seasonal cycle at the station closer to the coast. Results show that the two stations are not trophically different, although some controlling factors, such as zooplankton grazing in one case and light attenuation in the other, may further regulate the growth of phytoplankton. In both cases no significant trends are detected in either the average chlorophyll-a values or in dispersion of the data, in contrast with significant trends in temperature and salinity

NW Adriatic Sea biogeochemical variability in the last 20 years (1986–2005)

This paper presents a long-term time series (1986–2005) of hydrological and biogeochemical data, both published and unpublished. Data were collected in the north-western area of the Adriatic Sea, at two stations that are considered hydrodynamically and trophically different. The time series have been statistically and graphically analysed on a monthly scale in order to study the annual climatologies, links between the concentrations of chlorophyll-a and the variability in the environment, trophic differences between the two areas and chlorophyll-a trends over time. Basically, the two areas have similar hydrological features, yet they present significant differences in the amount of nutrient inputs: these are in fact higher at the coastal site, which is characterized by a prevalence of surface blooms, while they are lower at the offshore station, which is mainly affected by blooms at intermediate depths. Nonetheless, throughout the whole water column, chlorophyll-a concentrations are only slightly different. Both areas are affected by riverine discharge, though chlorophyll-a concentrations are also driven strongly by the seasonal cycle at the station closer to the coast. Results show that the two stations are not trophically different, although some controlling factors, such as zooplankton grazing in one case and light attenuation in the other, may further regulate the growth of phytoplankton. In both cases no significant trends are detected in either the average chlorophyll-a values or in dispersion of the data, in contrast with significant trends in temperature and salinity

Wastewater surveillance of SARS-CoV-2 variants in October–November 2022 in Italy: detection of XBB.1, BA.2.75 and rapid spread of the BQ.1 lineage

: This study adds insight regarding the occurrence and spread of SARS-CoV-2 Variants of Concern (VOCs) and Variants of Interest (VOIs) in Italy in October and November 2022, by testing urban wastewater collected throughout the country. A total of 332 wastewater samples were collected from 20 Italian Regions/Autonomous Provinces (APs) within the framework of national SARS-CoV-2 environmental surveillance. Of these, 164 were collected in the first week of October and 168 in the first week of November. A ∼1600 bp fragment of the spike protein was sequenced by Sanger (for individual samples) and long-read nanopore sequencing (for pooled Region/AP samples). In October, mutations characteristic of Omicron BA.4/BA.5 were detected in the vast majority (91 %) of the samples amplified by Sanger sequencing. A fraction of these sequences (9 %) also displayed the R346T mutation. Despite the low prevalence documented in clinical cases at the time of sampling, amino acid substitutions characteristic of sublineages BQ.1 or BQ.1.1 were detected in 5 % of sequenced samples from four Regions/APs. A significantly higher variability of sequences and variants was documented in November 2022, when the rate of sequences harbouring mutations of lineages BQ.1 and BQ1.1 increased to 43 %, and the number of Regions/APs positive for the new Omicron subvariant more than tripled (n = 13) compared to October. Moreover, an increase in the number of sequences with the mutation package BA.4/BA.5 + R346T (18 %), as well as the detection of variants never observed before in wastewater in Italy, such as BA.2.75 and XBB.1 (the latter in a Region where no clinical cases associated with this variant had ever been documented) was recorded. The results suggest that, as predicted by the ECDC, BQ.1/BQ.1.1 is rapidly becoming dominant in late 2022. Environmental surveillance proves to be a powerful tool for tracking the spread of SARS-CoV-2 variants/subvariants in the population

Nine-year nationwide environmental surveillance of hepatitis E virus in urban wastewaters in Italy (2011–2019)

Hepatitis E virus (HEV) is an emerging causative agent of acute hepatitis worldwide. To provide insights into the epidemiology of HEV in Italy, a large-scale investigation was conducted into urban sewage over nine years (2011–2019), collecting 1374 sewage samples from 48 wastewater treatment plants located in all the 20 regions of Italy. Broadly reactive primers targeting the ORF1 and ORF2 regions were used for the detection and typing of HEV, followed by Sanger and next generation sequencing (NGS). Real-time RT-qPCR was also used to attempt quantification of positive samples. HEV RNA detection occurred in 74 urban sewage samples (5.4%), with a statistically significant higher frequency (7.1%) in central Italy. Fifty-six samples were characterized as G3 strains and 18 as G1. While the detection of G3 strains occurred in all the surveillance period, G1 strains were mainly detected in 2011–2012, and never in 2017–2019. Typing was achieved in 2 samples (3f subtype). Viral concentrations in quantifiable samples ranged from 1.2 × 103 g.c./L to 2.8 × 104 g.c./L. Our results suggest the considerable circulation of the virus in the Italian population, despite a relatively small number of notified cases, a higher occurrence in central Italy, and a noteworthy predominance of G3 strains

Wastewater surveillance of SARS-CoV-2 variants in October-November 2022 in Italy: detection of XBB.1, BA.2.75 and rapid spread of the BQ.1 lineage

This study adds insight regarding the occurrence and spread of SARS-CoV-2 Variants of Concern (VOCs) and Var-iants of Interest (VOIs) in Italy in October and November 2022, by testing urban wastewater collected through-out the country. A total of 332 wastewater samples were collected from 20 Italian Regions/Autonomous Provinces (APs) within the framework of national SARS-CoV-2 environmental surveillance. Of these, 164 were collected in the first week of October and 168 in the first week of November. A similar to 1600 bp fragment of the spike protein was sequenced by Sanger (for individual samples) and long-read nanopore sequencing (for pooled Region/AP samples).In October, mutations characteristic of Omicron BA.4/BA.5 were detected in the vast majority (91 %) of the samples amplified by Sanger sequencing. A fraction of these sequences (9 %) also displayed the R346T mutation. Despite the low prevalence documented in clinical cases at the time of sampling, amino acid substitutions characteristic of sublineages BQ.1 or BQ.1.1 were detected in 5 % of sequenced samples from four Regions/APs. A significantly higher variability of sequences and variants was documented in November 2022, when the rate of se-quences harbouring mutations of lineages BQ.1 and BQ1.1 increased to 43 %, and the number of Regions/APs positive for the new Omicron subvariant more than tripled (n = 13) compared to October. Moreover, an increase in the number of sequences with the mutation package BA.4/BA.5 + R346T (18 %), as well as the detection of variants never observed before in wastewater in Italy, such as BA.2.75 and XBB.1 (the latter in a Region where no clinical cases asso-ciated with this variant had ever been documented) was recorded.The results suggest that, as predicted by the ECDC, BQ.1/BQ.1.1 is rapidly becoming dominant in late 2022. Environ-mental surveillance proves to be a powerful tool for tracking the spread of SARS-CoV-2 variants/subvariants in the population

Blood smear, a key diagnostic tool in hematology: Lessons from two cases of acute hemolysis in previously undiagnosed g6pd deficiency

Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common worldwide distributed hereditary red cells enzymatic defect. The most common life-threatening clinical presentation of G6PD deficiency is acute hemolytic crisis triggered by exposition to oxidative agents such as fava beans, drugs, or infections. Management of acute hemolytic crisis in patients with no previous history of hereditary red cell disorders is particularly challenging for hematologists and emergency department (ED) physicians. We report two cases of acute hemolytic crisis in patients with previously unknown G6PD deficiency, where blood smear analysis played a key role in decision-making process. The trigger of acute hemolytic crisis was in both cases the recent intake of large amount of fava beans. Although Case 1 typically involved a male subject with unknown G6PD deficiency, Case 2 is of particular interest since the patient is a female with previously unknown G6PD deficiency. A recent study highlights the possible appearance of clinical signs for G6PD deficiency with aging in elder female population. Thus, hematologists should always take into account the possible lyonization effect on G6PD activity for G6PD deficiency inherited red cell disorder in the presence of unexplained acute hemolytic crisis in women with circulating hemi-ghosts