Effects of Liposomes Contained in Thermosensitive Hydrogels as Biomaterials Useful in Neural Tissue Engineering

Indexación: Scopus.Advances in the generation of suitable thermosensitive hydrogels for the delivery of cells in neural tissue engineering demonstrate a delicate relationship between physical properties and capabilities to promote cell proliferation and differentiation. To improve the properties of these materials, it is possible to add liposomes for the controlled release of bioactive elements, which in turn can affect the physical and biological properties of the hydrogels. In the present investigation, different hydrogels based on Pluronic F127 have been formulated with the incorporation of chitosan and two types of liposomes of two different sizes. The rheological and thermal properties and their relation with the neurite proliferation and growth of the PC12 cell line were evaluated. Our results show that the incorporation of liposomes modifies the properties of the hydrogels dependent on the concentration of chitosan and the lipid type in the liposomes, which directly affect the capabilities of the hydrogels to promote the viability and differentiation of PC12 cells. © 2017 by the authors.http://www.mdpi.com/1996-1944/10/10/112

Application of biological based biomaterials, bioactive molecules and tissue engineering in periodontal plastic surgery: A review

Indexación: Web of Science; ScieloObjetivo: Describir, clasificar y discutir las indicaciones de los biomateriales de base biológica, moléculas bioactivas e ingeniería de tejidos que se están usando para el manejo de recesiones y aumento de encía en cirugía plástica periodontal. En esta revisión de la literatura, se utilizó una combinación de los términos de búsqueda específicos que consideraran los materiales en revisión, para el aumento de encía adherida, y el recubrimiento radicular. Materiales y Métodos: Se usaron las siguientes fuentes: Medline, Biblioteca Cochrane, y búsqueda manual de revistas específicas como el Journal of Periodontology, International Journal of Periodontics and Restorative Dentistry y Journal of Clinical Periodontology entre años 1985 y 2011. Se revisaron un total de 117 artículos y se seleccionaron 74 entre estudios clínicos controlados, estudios clínicos randomizados, reportes de casos y estudios en animales. Los artículos fueron revisados por los autores y aceptados por consenso para su discusión. Conclusiones: 1) Existe una serie de materiales que presentan gran potencial y podrían ser una alternativa viable a los injertos autógenos, pero se requiere más estudios a largo plazo. 2) Existe necesidad de estudios con la investigación de estos procedimientos en relación a resultados orientados a la estabilidad, seguridad y efectividad de los diferentes materiales existentes.Objective: To describe, classify and discuss the clinical applications of biologically based biomaterials, bioactive molecules and tissue engineering being utilized in gingival recession therapy and gingival augmentation procedures in plastic periodontal surgery. In this literature review, a combination of specific search key words were used, including materials being reviewed, indicated for gingival augmentation and root coverage procedures. Materials and Methods: The following sources were consulted: Medline, Cochrane Library and manual search of specific scientific journals such as Journal of Periodontology, International Journal of Periodontics and Restorative Dentistry and Journal of Clinical Periodontology between the years 1985 and 2011. A total of 117 articles were reviewed with 74 being selected unanimously by the authors for discussion in the manuscript. These articles included controlled clinical studies, randomized clinical studies, case reports and animal studies. The selected articles were reviewed by the authors and accepted by consensus. Conclusions: 1) There is a cohort of materials that exhibit great potential which could be a viable alternative to autografts but are in need of further long term studies. 2) There is a need of research of these materials in relation to stability, safety and efficacy.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0719-01072012000300009&nrm=is

Structure-activity relationships based on 3D-QSAR CoMFA/CoMSIA and design of aryloxypropanol-amine agonists with selectivity for the human β3-adrenergic receptor and anti-obesity and anti-diabetic profiles

Indexación: Scopus.Acknowledgments: This work was supported by FONDECYT No. 11130701. We would also like to thank fDoTr CthLeafbr efeora vthaeil afrbeilei tayvoafiltahbeilsitoyf towfa trheer seoqfutwireadret orecqaulciureladt etothcealAcuDla(thet ttph:e/ A/dDt c(lhatbt.pw:/e/dbstc.cloabm.w/seobfst.wcoamre/-stoofotlws aarned-tools and http://teqip.jdvu.ac.in/QSAR_Tools/). SDG. Conflicts of Interest: The authors declare no conflict of interest. Conflicts of Interest: The authors declare no conflict of interest.The wide tissue distribution of the adrenergic β3 receptor makes it a potential target for the treatment of multiple pathologies such as diabetes, obesity, depression, overactive bladder (OAB), and cancer. Currently, there is only one drug on the market, mirabegron, approved for the treatment of OAB. In the present study, we have carried out an extensive structure-activity relationship analysis of a series of 41 aryloxypropanolamine compounds based on three-dimensional quantitative structure-activity relationship (3D-QSAR) techniques. This is the first combined comparative molecular field analysis (CoMFA) and comparative molecular similarity index analysis (CoMSIA) study in a series of selective aryloxypropanolamines displaying anti-diabetes and anti-obesity pharmacological profiles. The best CoMFA and CoMSIA models presented values of r2 ncv = 0.993 and 0.984 and values of r2 test = 0.865 and 0.918, respectively. The results obtained were subjected to extensive external validation (q2, r2, r2 m, etc.) and a final series of compounds was designed and their biological activity was predicted (best pEC50 = 8.561). © 2018 by the authors.https://www.mdpi.com/1420-3049/23/5/119

White-tailed deer S96 prion protein does not support stable in vitro propagation of most common CWD strains

PrPC variation at residue 96 (G/S) plays an important role in the epidemiology of chronic wasting disease (CWD) in exposed white-tailed deer populations. In vivo studies have demonstrated the protective effect of serine at codon 96, which hinders the propagation of common CWD strains when expressed in homozygosis and increases the survival period of S96/wt heterozygous deer after challenge with CWD. Previous in vitro studies of the transmission barrier suggested that following a single amplification step, wt and S96 PrPC were equally susceptible to misfolding when seeded with various CWD prions. When we performed serial prion amplification in vitro using S96-PrPC, we observed a reduction in the efficiency of propagation with the Wisc-1 or CWD2 strains, suggesting these strains cannot stably template their conformations on this PrPC once the primary sequence has changed after the first round of replication. Our data shows the S96-PrPC polymorphism is detrimental to prion conversion of some CWD strains. These data suggests that deer homozygous for S96-PrPC may not sustain prion transmission as compared to a deer expressing G96-PrPC

Chronic wasting disease: a cervid prion infection looming to spillover

The spread of chronic wasting disease (CWD) during the last six decades has resulted in cervid populations of North America where CWD has become enzootic. This insidious disease has also been reported in wild and captive cervids from other continents, threatening ecosystems, livestock and public health. These CWD “hot zones” are particularly complex given the interplay between cervid PRNP genetics, the infection biology, the strain diversity of infectious prions and the long-term environmental persistence of infectivity, which hinder eradication efforts. Here, we review different aspects of CWD including transmission mechanisms, pathogenesis, epidemiology and assessment of interspecies infection. Further understanding of these aspects could help identify “control points” that could help reduce exposure for humans and livestock and decrease CWD spread between cervids

Determinantes del precio de la vivienda nueva No VIS en Medellín: un modelo estructural

Esta investigación a diferencia de otras realizadas en el sector busca a través de la utilización de un modelo de ecuaciones estructurales, en el cual se utilizan variables observables y no observables, identificar los determinantes del precio de la vivienda nueva No VIS en Medellín, entre 2009-2014, con el fin de explicar la formación de precios desde la organización industrial del sector. Los resultados indican que el empleo, el crecimiento económico y las políticas públicas (subsidios) influyen directamente –la inflación tiene injerencia negativa– sobre las condiciones del mercado de vivienda (CMV); simultáneamente, las CMV afectan la oferta y la demanda, fuerzas que, a su vez, inciden en la formación de los precios de mercado. Los principales hallazgos de la investigación se centran en la consolidación de un esquema que permite integrar la interdependencia entre variables para la explicación de la formación de precios y la identificación de la incidencia de las políticas públicas en el aumento de los precios.We employ a multi-equation structural model of the housing market to analyze the determinants of the price of new housing in Medellin during 2009-2014. The model includes observed variables and unobserved variables, such as costs that are not included in the housing cost index but that still affect prices. In the analysis we employ an industrial organization framework to examine the interaction between microeconomic and macroeconomic variables. The results indicate that employment, economic growth, and public policies (subsidies) affect real housing prices positively, while inflation affects real prices negatively. In addition to the variables typically included in the analysis in prior research, we include several determinants of housing supply, including construction costs, and two market interest rates

Obtention of Antimicrobial Fibers Type Core/Shell PLA/PVOH-LAE by Coaxial Electrospinning

Coaxial electrospinning (EC) is a technology that allows the encapsulation of active compounds, as ethyl lauroyl arginate (LAE), in shell/core structures, in order to develop new antimicrobial food packaging materials with slowdown of active compound´s release with the purpose of extending food shelf life. For this reason, the objective of this study consisted in developing antimicrobial fibers shell/core type by EC. Two polymers with different hydrophilic character as poly (lactic acid) (PLA) to the shell and poly (vinyl alcohol) (PVOH) and LAE to the core were used to obtain PLA/PVOH-LAE fibers, and slowdown the release of antimicrobial compound. The morphology of fibers was evaluated by optical microscopy and the thermal properties through thermogravimetric analyses (TGA). Release studies were carried out in fatty (ETOH 95%) food simulant and was compared with the minimum inhibitory concentration (MIC) values of LAE against Listeria innnocua. The optical micrographs evidenced the obtention of shell/core structure with an average diameter of 0,6 µm approximately, and the TGA analyses demonstrated the thermal protection of LAE by shell of fibers. Released LAE reached the equilibrium state in ETOH 95% during the first 3 hours, maintaining a higher concentration than MIC value obtained in L. innnocua (10 ppm). The results demonstrate that new packaging materials with antimicrobial activity as PLA/PVOH-LAE polymeric fibers with shell/core structure can be obtained through coaxial electrospinning technique. Keywords: coaxial electrospinning, ethyl lauroyl arginate, fibers, shell/core, food packaging. Resumen El electrohilado coaxial (EC) es una tecnología que permite encapsular compuestos activos como el etil lauroil arginato (LAE) en estructuras tipo pared/núcleo con la finalidad de desarrollar nuevos materiales antimicrobianos para el envasado de alimentos que ralenticen la liberación del compuesto y extiendan la vida útil del alimento. Por tal razón, el objetivo de este estudio consistió en desarrollar fibras antimicrobianas tipo pared/núcleo mediante EC. Dos polímeros con distinto carácter hidrofílico como el poli (ácido láctico) (PLA) para la pared y poli (alcohol vinílico) (PVOH) y LAE para el núcleo se utilizaron para obtener las fibras PLA/PVOH-LAE, y ralentizar la liberación del compuesto antimicrobiano. La morfología de las fibras se evaluó mediante microscopía óptica y sus propiedades térmicas mediante análisis termogravimétricos (TGA). Se realizaron estudios de liberación del LAE en un simulante de alimentos graso (ETOH 95%), y se comparó con la concentración mínima inhibitoria (MIC) hacia una bacteria Gram positiva Listeria innnocua. Las micrografías ópticas evidenciaron la obtención de la estructura pared/núcleo con un diámetro promedio de 0,6 µm aproximadamente, y el análisis TGA demostró la protección térmica del LAE por la pared de las fibras. El LAE liberado alcanzó el estado de equilibrio en ETOH 95% durante las 3 hr iniciales, manteniendo una concentración superior a la MIC obtenida para L. innnocua (10 ppm). Los resultados demuestran que nuevos materiales de envase con actividad antimicrobiana como fibras poliméricas PLA/PVOH-LAE con estructura pared/núcleo pueden ser obtenidos mediante la técnica de electrohilado coaxial. Palabras clave: electrohilado coaxial, etil lauroil arginato, fibras, pared/núcleo, envase de alimentos

Obtention of Antimicrobial Fibers Type Core/Shell Pla/Pvoh-Lae By Coaxial Electrospinning

Coaxial electrospinning (EC) is a technology that allows the encapsulation of active compounds, such as ethyl lauroyl arginate (LAE), in shell/core structures, in order to develop new antimicrobial materials for food packaging that slow down the release of active compounds and extend the food’s shelf life. For this reason, the objective of this study was to develop antimicrobial fibers shell/core type by EC. Two polymers with different hydrophilic character, polylactic acid (PLA) for the shell and polyvinyl alcohol (PVOH) and LAE for the core, were used to obtain PLA/PVOH-LAE fibers and slow the release of the antimicrobial compound. The morphology of fibers was evaluated by optical microscopy and their thermal properties through thermogravimetric analyses (TGA). LAE release studies were carried out in a fatty food simulant (ETOH 95%), and was compared with the minimum inhibitory concentration (MIC) values of LAE against a gram-positive bacteria, Listeria innnocua. The optical micrographs showed the obtaining of the shell/core structure with an average diameter of approximately 0.6 µm, and the TGA analyses demonstrated the thermal protection of LAE by the shell of the fibers. Released LAE reached the equilibrium state in ETOH 95% during the first 3 hours, maintaining a higher concentration than the MIC value obtained in L. innnocua (10 ppm). The results demonstrate that new packaging materials with antimicrobial activity such as PLA/PVOH-LAE polymeric fibers with a shell/core structure can be obtained through the coaxial electrospinning technique. Keywords: coaxial electrospinning, ethyl lauroyl arginate, fibers, shell/core, food packaging. Resumen El electrohilado coaxial (EC) es una tecnología que permite encapsular compuestos activos como el etil lauroil arginato (LAE) en estructuras tipo pared/núcleo con la finalidad de desarrollar nuevos materiales antimicrobianos para el envasado de alimentos que ralenticen la liberación del compuesto y extiendan la vida útil del alimento. Por tal razón, el objetivo de este estudio consistió en desarrollar fibras antimicrobianas tipo pared/núcleo mediante EC. Dos polímeros con distinto carácter hidrofílico como el poli (ácido láctico) (PLA) para la pared y poli (alcohol vinílico) (PVOH) y LAE para el núcleo se utilizaron para obtener las fibras PLA/PVOH-LAE, y ralentizar la liberación del compuesto antimicrobiano. La morfología de las fibras se evaluó mediante microscopía óptica y sus propiedades térmicas mediante análisis termogravimétricos (TGA). Se realizaron estudios de liberación del LAE en un simulante de alimentos graso (ETOH 95%), y se comparó con la concentración mínima inhibitoria (MIC) hacia una bacteria Gram positiva Listeria innnocua. Las micrografías ópticas evidenciaron la obtención de la estructura pared/núcleo con un diámetro promedio de 0,6 µm aproximadamente, y el análisis TGA demostró la protección térmica del LAE por la pared de las fibras. El LAE liberado alcanzó el estado de equilibrio en ETOH 95% durante las 3 hr iniciales, manteniendo una concentración superior a la MIC obtenida para L. innnocua (10 ppm). Los resultados demuestran que nuevos materiales de envase con actividad antimicrobiana como fibras poliméricas PLA/PVOH-LAE con estructura pared/núcleo pueden ser obtenidos mediante la técnica de electrohilado coaxial. Palabras clave: electrohilado coaxial, etil lauroil arginato, fibras, pared/núcleo, envase de alimentos

Exact inflationary solutions

We present a new class of exact inflationary solutions for the evolution of a universe with spatial curvature, filled with a perfect fluid, a scalar field with potential $V_{\pm}(\phi)=\lambda(\phi^2\pm\delta^2)^2$ and a cosmological constant $\Lambda$. With the $V_+(\phi)$ potential and a negative cosmological constant, the scale factor experiments a graceful exit. We give a brief discussion about the physical meaning of the solutions.Comment: 10 pages, revtex file, 6 figures included with epsf. To be published in IJMP-

Effects of discontinued endurance methods on VO2max in judokas

Este estudio tuvo como objetivo investigar el efecto de dos diferentes planes de entrenamiento de resistencia basados en la metodología discontinua (intermitente e interválica), sobre el VO2Máx de atletas Antioqueños de Judo de rendimiento. Para ello, 21 Judokas de la Selección Antioqueña pertenecientes a la categoría Senior (20,43±4,18 años), fueron distribuidos aleatoriamente en tres grupos, dos grupos experimentales y un grupo control. Ambos grupos experimentales entrenaron la resistencia durante 4 semanas con 4 estímulos por semana, uno de los grupos experimentales basó su entrenamiento en el método interválico y el otro en el intermitente. Por su parte, el grupo control no realizó ningún tipo de entrenamiento de la resistencia. No se hallaron diferencias significativas entre pretest y postest de ninguno de los grupos ni experimentales ni el de grupo control (p>0,05). Se concluyó que el entrenamiento de la resistencia basado en métodos discontinuos no produjo diferencias significativas en el VO2Máx