Nitrogen enrichment suppresses other environmental drivers and homogenizes salt marsh leaf microbiome

Microbial community assembly is affected by a combination of forces that act simultaneously, but the mechanisms underpinning their relative influences remain elusive. This gap strongly limits our ability to predict human impacts on microbial communities and the processes they regulate. Here, we experimentally demonstrate that increased salinity stress, food web alteration and nutrient loading interact to drive outcomes in salt marsh fungal leaf communities. Both salinity stress and food web alterations drove communities to deterministically diverge, resulting in distinct fungal communities. Increased nutrient loads, nevertheless, partially suppressed the influence of other factors as determinants of fungal assembly. Using a null model approach, we found that increased nutrient loads enhanced the relative importance of stochastic over deterministic divergent processes; without increased nutrient loads, samples from different treatments showed a relatively (deterministic) divergent community assembly whereas increased nutrient loads drove the system to more stochastic assemblies, suppressing the effect of other treatments. These results demonstrate that common anthropogenic modifications can interact to control fungal community assembly. Furthermore, our results suggest that when the environmental conditions are spatially heterogeneous (as in our case, caused by specific combinations of experimental treatments), increased stochasticity caused by greater nutrient inputs can reduce the importance of deterministic filters that otherwise caused divergence, thus driving to microbial community homogenization.Fil: Daleo, Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Alberti, Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Jumpponen, Ari. Kansas State University; Estados UnidosFil: Veach, Allison. Kansas State University; Estados UnidosFil: Ialonardi, Florencia Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Iribarne, Oscar Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Silliman, Brian Red. University Of Duke. Nicholas School Of Environment. Marine Science And Conservation División; Estados Unido

Measurements of the Temperature and E-Mode Polarization of the CMB from 500 Square Degrees of SPTpol Data

We present measurements of the $E$-mode polarization angular auto-power spectrum ($EE$) and temperature-$E$-mode cross-power spectrum ($TE$) of the cosmic microwave background (CMB) using 150 GHz data from three seasons of SPTpol observations. We report the power spectra over the spherical harmonic multipole range $50 < \ell \leq 8000$, and detect nine acoustic peaks in the $EE$ spectrum with high signal-to-noise ratio. These measurements are the most sensitive to date of the $EE$ and $TE$ power spectra at $\ell > 1050$ and $\ell > 1475$, respectively. The observations cover 500 deg$^2$, a fivefold increase in area compared to previous SPTpol analyses, which increases our sensitivity to the photon diffusion damping tail of the CMB power spectra enabling tighter constraints on \LCDM model extensions. After masking all sources with unpolarized flux $>50$ mJy we place a 95% confidence upper limit on residual polarized point-source power of $D_\ell = \ell(\ell+1)C_\ell/2\pi <0.107\,\mu{\rm K}^2$ at $\ell=3000$, suggesting that the $EE$ damping tail dominates foregrounds to at least $\ell = 4050$ with modest source masking. We find that the SPTpol dataset is in mild tension with the $\Lambda CDM$ model ($2.1\,\sigma$), and different data splits prefer parameter values that differ at the $\sim 1\,\sigma$ level. When fitting SPTpol data at $\ell < 1000$ we find cosmological parameter constraints consistent with those for $Planck$ temperature. Including SPTpol data at $\ell > 1000$ results in a preference for a higher value of the expansion rate (H_0 = 71.3 \pm 2.1\,\mbox{km}\,s^{-1}\mbox{Mpc}^{-1} ) and a lower value for present-day density fluctuations ($\sigma_8 = 0.77 \pm 0.02$).Comment: Updated to match version accepted to ApJ. 34 pages, 17 figures, 6 table

The Balloon Experimental Twin Telescope for Infrared Interferometry (BETTII): towards the first flight

The Balloon Experimental Twin Telescope for Infrared Interferometry (BETTII) is a balloon-borne, far-infrared direct detection interferometer with a baseline of 8 m and two collectors of 50 cm. It is designed to study galactic clustered star formation by providing spatially-resolved spectroscopy of nearby star clusters. It is being assembled and tested at NASA Goddard Space Flight Center for a first flight in Fall 2016. We report on recent progress concerning the pointing control system and discuss the overall status of the project as it gets ready forits commissioning flight

Fourier Analysis of Correlated Monte Carlo Importance Sampling

International audienceFourier analysis is gaining popularity in image synthesis, as a tool for the analysis of error in Monte Carlo (MC) integration. Still, existing tools are only able to analyze convergence under simplifying assumptions (such as randomized shifts) which are not applied in practice during rendering. We reformulate the expressions for bias and variance of sampling-based integrators to unify non-uniform sample distributions (importance sampling) as well as correlations between samples while respecting finite sampling domains. Our unified formulation hints at fundamental limitations of Fourier-based tools in performing variance analysis for MC integration. This non-trivial exercise also provides exciting insight into the effects of importance sampling on the convergence rate of estimators because of the introduction or removal of discontinuities. Specifically, we demonstrate that the convergence of multiple importance sampling (MIS) is determined by the strategy that converges slowest. We propose two simple and practical approaches to limit the impact of discontinuities on the convergence rate of estimators: The first one involves mirroring the integrand to cancel out the effect of boundary discontinuities. This is followed by two novel mirror sampling techniques for MC estimation in this mirrored domain. The second approach improves direct illumination light sampling by smoothing out discontinuities within the domain at the cost of introducing a small amount of bias. Our approaches are simple, practical and can be easily incorporated in production renderers

The Balloon Experimental Twin Telescope for Infrared Interferometry (BETTII): First Flight

The Balloon Experimental Twin Telescope for Infrared Interferometry (BETTII) is an 8-meter far-infrared (30-100 m) double-Fourier Michelson interferometer designed to fly on a high altitude scientific balloon. The project began in 2011, and the payload was declared ready for flight in September 2016. Due to bad weather, the first flight was postponed until June 2017; BETTII was successfully launched on June 8, 2017 for an engineering flight. Over the course of the one night flight, BETTII acquired a large amount of technical data that we are using to characterize the payload. Unfortunately, the flight ended with an anomaly that resulted in destruction of the payload. In this paper, we will discuss the path to BETTII flight, the results of the first flight, and some of the plans for the future

TGF-beta(2)- and H2O2-Induced Biological Changes in Optic Nerve Head Astrocytes Are Reduced by the Antioxidant Alpha-Lipoic Acid

Background/Aims: The goal of the present study was to determine whether transforming growth factor-beta(2) (TGF-beta(2))- and oxidative stress-induced cellular changes in cultured human optic nerve head (ONH) astrocytes could be reduced by pretreatment with the antioxidant alpha-lipoic acid (LA). Methods: Cultured ONH astrocytes were treated with 1.0 ng/ml TGF-beta(2) for 24 h or 200 mu M hydrogen peroxide (H2O2) for 1 h. Lipid peroxidation was measured by a decrease in cis-pari-naric acid fluorescence. Additionally, cells were pretreated with different concentrations of LA before TGF-beta 2 or H2O2 exposure. Expressions of the heat shock protein (Hsp) alpha B-crystallin and Hsp27, the extracellular matrix (ECM) component fibronectin and the ECM-modulating protein connective tissue growth factor (CTGF) were examined with immunohistochemistry and real-time PCR analysis. Results: Both TGF-beta(2) and H2O2 increased lipid peroxidation. Treatment of astrocytes with TGF-beta(2) and H2O2 upregulated the expression of alpha B-crystallin, Hsp27, fibronectin and CTGF. Pretreatment with different concentrations of LA reduced the TGF-beta(2)- and H2O2-stimulated gene expressions. Conclusion: We showed that TGF-beta(2)- and H2O2-stimulated gene expressions could be prevented by pretreatment with the antioxidant LA in cultured human ONH astrocytes. Therefore, it is tempting to speculate that the use of antioxidants could have protective effects in glaucomatous optic neuropathy. Copyright (C) 2012 S. Karger AG, Base

Constraints on cosmological parameters from the 500 deg2 SPTPOL lensing power spectrum

We present cosmological constraints based on the cosmic microwave background (CMB) lensing potential power spectrum measurement from the recent 500 deg2 SPTpol survey, the most precise CMB lensing measurement from the ground to date. We fit a flat ΛCDM model to the reconstructed lensing power spectrum alone and in addition with other data sets: baryon acoustic oscillations (BAO), as well as primary CMB spectra from Planck and SPTpol. The cosmological constraints based on SPTpol and Planck lensing band powers are in good agreement when analyzed alone and in combination with Planck full-sky primary CMB data. With weak priors on the baryon density and other parameters, the SPTpol CMB lensing data alone provide a 4% constraint on ${\sigma }_{8}{{\rm{\Omega }}}_{m}^{0.25}=0.593\pm 0.025$. Jointly fitting with BAO data, we find ${\sigma }_{8}=0.779\pm 0.023$, ${{\rm{\Omega }}}_{m}={0.368}_{-0.037}^{+0.032}$, and ${H}_{0}={72.0}_{-2.5}^{+2.1}\,\mathrm{km}\,{{\rm{s}}}^{-1}\,{\mathrm{Mpc}}^{-1}$, up to $2\sigma$ away from the central values preferred by Planck lensing + BAO. However, we recover good agreement between SPTpol and Planck when restricting the analysis to similar scales. We also consider single-parameter extensions to the flat ΛCDM model. The SPTpol lensing spectrum constrains the spatial curvature to be ${{\rm{\Omega }}}_{K}=-0.0007\pm 0.0025$ and the sum of the neutrino masses to be $\sum {m}_{\nu }\lt 0.23$ eV at 95% C.L. (with Planck primary CMB and BAO data), in good agreement with the Planck lensing results. With the differences in the signal-to-noise ratio of the lensing modes and the angular scales covered in the lensing spectra, this analysis represents an important independent check on the full-sky Planck lensing measurement