Condition of the liver’s liver of rats, received a high-fat diet: biochemical indicators

Aim: To determine the liver condition of rats fed with fats of different fatty acid compositions Materials and methods: We used the following dietary fats: refined sunflower oil, refined olive oil, palm oil, milk fat (butter). Feeding of rats was carried out for 41 days using 15 % of the studied oils in the food. The liver condition was evaluated by biochemical markers of liver (elastase, MDA, alkaline phosphatase (ALP), lysozyme, catalase) and blood serum markers (glucose, cholesterol, elastase, ALT, ALP). Results: The increase in the weight gain was more pronounced when feeding with palm oil; the increase of inflammatory markers levels (MDA and elastase) was greater when feeding with palm oil and butter. Conversely, the lysozyme activity in the liver was significantly reduced at fat feeding, particularly for palm oil and butter. In the serum of all animals fed with fat there was the significant increase of glucose and cholesterol levels, as well as the increase of the elastase activity (after palm oil). Conclusion: A high-fat diet causes the development of an initial phase of inflammatory and degenerative processes in liver, which is more pronounced for fats high in palmitinic acid (palm oil and butter)

Профилактика ожирения и неалкогольного стеатогепатита у крыс антидисбиотическим средством = Prevention and treatment effect of antidysbiotic formulation on obesity and nonalcoholic steatohepatitis in rats

Levitsky A. P., Tkachuk V. V., Velichko V. I., Vasyuk V. L. Профилактика ожирения и неалкогольного стеатогепатита у крыс антидисбиотическим средством = Prevention and treatment effect of antidysbiotic formulation on obesity and nonalcoholic steatohepatitis in rats. Journal of Education, Health and Sport. 2016;6(4):281-286. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.50200 http://ojs.ukw.edu.pl/index.php/johs/article/view/3471 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015). 755 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Author (s) 2016; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 20.03.2016. Revised 17.04.2016. Accepted: 17.04.2016. УДК 517.112:612.8+615.462 ПРОФИЛАКТИКА ОЖИРЕНИЯ И НЕАЛКОГОЛЬНОГО СТЕАТОГЕПАТИТА У КРЫС АНТИДИСБИОТИЧЕСКИМ СРЕДСТВОМ А. П. Левицкий1, В. В. Ткачук2, В. И. Величко2, В. Л. Васюк3 1ГУ «Институт стоматологии НАМН Украины» (г. Одесса, Украина) e-mail: [email protected] 2Одесский национальный медицинский университет (Украина) 3Буковинский государственный медицинский университет (г. Черновцы, Украина) Резюме Сочетание высокожирового рациона и кишечного дисбиоза вызывает у крыс развитие ожирения и неалкогольного стеатогепатита (НАСГ). Введение антидисбиотического средства «Квертулин» (кверцетин + инулин + цитрат кальция) предотвращает развитие ожирения и НАСГ. Ключевые слова: ожирение, неалкогольный стеатогепатит, липиды, высокожировое питание, дисбиоз, антидисбиотическое средство. PREVENTION AND TREATMENT EFFECT OF ANTIDYSBIOTIC FORMULATION ON OBESITY AND NONALCOHOLIC STEATOHEPATITIS IN RATS A. P. Levitsky1, V. V. Tkachuk2, V. I. Velichko2, V. L. Vasyuk3 1SE«The Institute of Stomatology of the National academie of medical science of Ukraine» (Odessa, Ukraine) e-mail: [email protected] 2Odessa National Medical University (Ukraine) 3Bukovina State Medical University (Chernovtsy, Ukraine) Abstract Aim: To determine of prevention and treatment effect of antidysbiotic formulation on obesity and NASH in rats. Methods: The experimental obesity and NASH were induced by feeding rats with a high fat diet (HFD) (fodder, plus 15 % sunflower oil – 40 days) and driaking water with lincomycin at a dose of 60 mg/kg day for 5 days. The duration of experiment was 41 days. One group of rats received HFD and lincomycin, received antidysbiotic formulation Quertulin (quercetine + inuline + Ca-citrate) in dose 300 mg/kg 40 days. Determined morphometric parameters like body mass, body mass index, content of triglyceride (TG) and cholesterine (Ch) of liver and serum cjntent of bilirubine and activity of ALT and in serum, alkaline phosphatase (AP). Results: At obesity and NASH body mass, body mass index, content of TR, Ch, in liver and serum were increased; in serum content to bilirubine and activity to ALT and AP were increased. Taking quertulin normalized morphometric and biochemic indexis in liver and serum. Conclusion: Antidysbiotic formulation quertulin made of prevention and treatment effect on obesity and NASH. Key words: obesity, nonalcoholic steatohepatitis, lipids, high fat nutrition, dysbiosis, antidysbiotic formulation

Oxidation resistance of nano-reinforced PC-refractories modified with phenol formaldehyde resin. Part 4. Thermodynamic evaluation of phase formation within Mg–O–C–Al, Mg–O–C–Ni and МgO‒Al₂O₃‒NiO‒SiO₂ systems using SiC + Al + Ni (NiO) complex antioxidant

Results are given for the synthesis and co-existence of phases formed from components of complex organic- inorganic antioxidant formed during modification of phenol-formaldehyde resin (PFR) and graphite with silica alkoxide and inorganic or organic nickel precursors. Thermodynamic analysis is given for the Mg–Al–C and Mg–O–Ni–C systems. It is shown that the periclase and carbon can coexist with aluminum and nickel, and also that oxidized antioxidants Al₂O₃ and NiO can interact respectively with the periclase and with the synthesized SiC formed during modification of PFR with silica. In considering the NiO‒MgO‒Al₂O₃‒SiO₂ system it is established that during service noble spinel will be synthesized from the complex antioxidant components, facilitating an increase in PC-refractory durability in service

Quantitative determination of 4-((5-(decylthio)-4-methyl-4-h-1,2,4-triazol-3-yl)methyl)morpholine in the soft medicinal form by spectrophotometric method

Synthetic biologically active compounds are the active pharmaceutical ingredients of various dosage forms that are widely used in both human and veterinary medicine. Nowadays the development of new effective antimicrobial and antifungal drugs remains a actual problem. This fact has a reasoned explanation in connection with the increase in infectious diseases of microbial and fungal etiology and the ever-growing resistance of microorganisms and fungi to various drugs. The aim of this research was to develop and validate a new spectrophotometric method for the quantitative determination of 4-((5-(decylthio)-4-methyl-4-H-1,2,4-triazol-3-yl)methyl)morpholine in 7% ointment by intrinsic absorption. At the stage of development of the quantitative determination method, the solvent was selected based on the solubility of the active substance. According to the data provided, 4-((5-(decylthio)-4-methyl-4-H-1,2,4-triazol-3-yl)methyl)morpholine is insoluble in water, but soluble in ethanol, methanol, isopropanol, dioxane, and dimethyl sulfoxide. Ethanol was chosen as the most accessible and less toxic solvent for the development of this method. Studied 4-((5-(decylthio)-4-methyl-4-H-1,2,4-triazol-3-yl)methyl)morpholine dissolved in ethanol is introduced into the ointment base as a solution. The quantitative content of substances subject to chemical control in soft dosage forms should be between 90% and 110% of the nominal content. On this basis, the maximum permitted analytical uncertainty can be determined, which is 3.20. During the procedure the main validation characteristics were determined, such as: linearity, precision, accuracy and robustness. Thus, a new sensitive, economical and easy-to-use spectrophotometric method for the quantitative determination of 4-((5-(decylthio)-4-methyl-4-H-1,2,4-triazol-3-yl)methyl)morpholine in a soft dosage form for external use (7% ointment) by the intrinsic absorption method was developed. According to the main validation characteristics (linearity, precision, accuracy and robustness), the proposed method is valid and meets the requirements of the State Pharmacopoeia of Ukraine

ОПРЕДЕЛЕНИЕ КОНЦЕПЦИИ КАЧЕСТВА ФИЗИЧЕСКОГО СОВЕРШЕНСТВОВАНИЯ ЧЕЛОВЕКА В КОНТЕКСТЕ КАЧЕСТВА ЖИЗНИ

The urgency of definition of the concept of quality of physical perfection in a context of quality of life is described. Necessity of application to the decision of the given problem of system approaches is shown. The structure of developed model of system of quality management of granting of service as complex of the interconnected systems is defined. Questions of maintenance of a complex of means and methods of an estimation of a condition of the client and definition of a technique of formation of the correct typical program of physical preparation of the client are allocated. Описана актуальность определения концепции качества физического совершенствования в контексте качества жизни. Показана необходимость применения к решению данной проблемы системных подходов. Определена структура разрабатываемой модели системы менеджмента качества предоставления услуги как комплекса взаимосвязанных систем. Выделены вопросы обеспечения комплекса средств и методов оценки состояния клиента и определения методики формирования корректной типовой программы физической подготовки клиента.

ПОРІВНЯЛЬНА ХАРАКТЕРИСТИКА НАКІСТКОВОГО ОСТЕОСИНТЕЗУ ПРИ ПЕРЕЛОМАХ ПЛЕЧОВОЇ КІСТКИ

The authors adduce the results of a clinical case follow-up over 61 patients with fractures of the humerus who have been treated, using external fixation. It has been demonstrated that patients operated by means of nail-plate fixation with angular stability were characterized by such higher parameters of the postoperative condition: the evidence of strength arm flexing, the ability to abduct the arm, external and internal rotation, evidencing of better indices of the quality of life in this particular cohort of patients and the advantages of the technology in question.Приведены результаты клинического наблюдения за 61 больным с переломами плечевой кости, которых пролечено с применением накостного остеосинтеза. У пациентов, прооперированных с использованием остеосинтеза пластинами с угловой стабильностью, выше оказались следующие показатели послеоперационного состояния: выраженность силы, сгибание руки, способность отведения руки, наружная и внутренняя ротация, что свидетельствует о лучших показателях качества жизни больных и преимущества указанной технологии.Наведені результати динамічного клінічного спостереження за 61 хворим з переломами плечової кістки, яких проліковано із застосуванням накісткового остеосинтезу. Показано, що в пацієнтів, прооперованих з використанням остеосинтезу пластинами з кутовою стабільністю, вищими виявилися такі показники післяопераційного стану: вираженість сили, згинання руки, здатність відводити руку, зовнішня та внутрішня ротація, що свідчить про кращі показники якості життя у даної категорії хворих та переваги вказаної технології

PREDICTIVE VALUE OF ECHOCARDIOGRAPHY IN POST MYOCARDIAL INFARCTION SETTING. PART 2

Echocardiography is a useful tool for risk stratification and prognosis assessment after myocardial infarction. It was shown, that for prediction related data acquisition, it is possible to apply multiple echocardiographic parameters, such as the volumes and ejection fraction of the left ventricle, wall motion index, left atrium volume, and existence of atrial regurgitation. Development of the method of tissue Doppler and “speckle-tracking” led to invention of novel prediction parameters, as deformation, deformation velocity, dissynchrony of the left ventricle. Method of contrast echocardiography makes it to evaluate myocardial perfusion and safety of microvascularity, gives valuable data on myocardial viability, which is closely related to prognosis. Stress echocardiography makes it to assess myocardial ischemia and find viable myocardium, and the Doppler of coronary arteries — to evaluate coronary flow reserve. Finally, 3D echo makes possible the gathering of optimal data on the volumes, functioning and sphericity of the left ventricle, which are significant parameters of long term prognosis

Використання 2,3-дихлор-1,4-нафтохінону для спектрофотометричного визначення ацетилцистеїну в лікарських препаратах

The aim of research was the development and validation ofspectrophotometric method foracetylcysteine assay in pharmaceutical formulations.Тhe proposed method is based on the reaction with 2,3-dichloro-1,4-naphthoquinone and the formation of colored products that exhibit absorption maxima at 425 nm.IntroductionMany analytical methods have been published for acetylcysteine assay in pharmaceutical formulations as high-performance liquid chromatography (HPLC), fluorimetry and chemiluminescence. Some of these methods are time consuming or require expensive equipment. Other published methods suffer from lack of selectivity and sensitivity. Spectrophotometry is the most widely used technique in pharmaceutical analysis because it is simple, economic, and easily available to most quality control laboratories.In the present work, we propose a simple and accurate spectrophotometric method for acetylcysteine assay in pharmaceutical formulations.Materials and MethodsReagents: Reference standard acetylcysteinesubstance; 2,3-dichloro-1,4-naphthoquinone. All chemicals and solvents were of analytical grade.DMFA was used as a solvent.Pharmaceutical preparations:powder for oral solution «ACC 200» 200 mgseries number50026151 (Salutas Pharma CmbH, Germany); effervescent tablets  «Fluimucil» 600 mg (Zambon S.P.A., Italy) and «ACC LONG» 600 mg (Salutas Pharma CmbH, Germany) series numbers 321284 and DH2740; solution for injections «Fluimucil» 100 mg/ml (Zambon S.P.A., Italy)series number28002492.Solutions:Acetylcysteine stock solution (0,16%);DMFAsolution of 2,3-dichloro-1,4-naphthoquinone (4%).EquipmentAnalytical balance (ABT-120-5DM); UV-VIS spectrophotometer (Specord 200); water bath (MemmertWNB 7-45);quartz cells.ResultsAcetylcysteine was determined using a spectrophotometric method based on the reaction with 2,3-dichloro-1,4-naphthoquinone to form yellow colored reaction products with absorption maxima at 425 nm. The effect of reaction time and temperature was studied. It was found that the reaction requires heating at 95°С for 10 min.The proposed method is valid according to the validation requirements of Ukrainian Pharmacopeia. The following parameters were considered: range of application, linearity, accuracy, robustness, precision of the results.ConclusionThe proposed spectrophotometric method for quantitative determination of acetylcysteine in pharmaceutical formulations was found to be simple, precise, accurate, linear, robust and rugged during validation. Further this method can be recommended for routine quantitative determination of the studied drugs in quality control laboratories.Актуальной проблемой современного фармацевтического анализа является создание эффективных и экономичных методик количественного определения лекарственных веществ. Цель работы – разработка и валидация новой спектрофотометрической методики количественного определения ацетилцистеина в лекарственных формах. Установлено, что ацетилцистеин реагирует с 2,3-дихлор-1,4-нафтохиноном с образованием окрашенного продукта реакции с максимумом абсорбции при 425 нм. Предложенная методика отвечает требованиям Государственной фармакопеи Украины, предъявляемым к методикам количественного анализа лекарственных веществ. Результаты исследования показывают, что методика является высокочувствительной, точной, простой в выполнении и пригодной для использования в лабораториях Государственной инспекции по контролю качества лекарственных веществ, а также отделах технического контроля химико-фармацевтических предприятий.Актуальною проблемою сучасного фармацевтичного аналізу є створення ефективних та економічних методик кількісного визначення лікарських речовин. Мета роботи полягала в розробці та валідації нової спектрофотометричної методики кількісного визначення ацетилцистеїну в лікарських формах. Встановили, що ацетилцистеїн реагує з 2,3-дихлор-1,4-нафтохіноном з утворенням забарвленого продукту реакції з максимумом абсорбції при 425 нм. Запропонована методика відповідає вимогам Державної фармакопеї України щодо методик кількісного аналізу лікарських речовин. Результати дослідження свідчать, що методика є високочутливою, точною, простою у виконанні та придатною для використання в лабораторіях Державної інспекції з контролю якості лікарських речовин, а також відділах технічного контролю хіміко-фармацевтичних підприємств