A standalone bioreactor system to deliver compressive load under perfusion flow to hBMSC-seeded 3D chitosan-graphene templates

The availability of engineered biological tissues holds great potential for both clinical applications and basic research in a life science laboratory. A prototype standalone perfusion/compression bioreactor system was proposed to address the osteogenic commitment of stem cells seeded onboard of 3D chitosan-graphene (CHT/G) templates. Testing involved the coordinated administration of a 1 mL/min medium flow rate together with dynamic compression (1% strain at 1 Hz; applied twice daily for 30 min) for one week. When compared to traditional static culture conditions, the application of perfusion and compression stimuli to human bone marrow stem cells using the 3D CHT/G template scaffold induced a sizable effect. After using the dynamic culture protocol, there was evidence of a larger number of viable cells within the inner core of the scaffold and of enhanced extracellular matrix mineralization. These observations show that our novel device would be suitable for addressing and investigating the osteogenic phenotype commitment of stem cells, for both potential clinical applications and basic research

The mitochondrial probe rhodamine 123 inhibits in isolated hepatocytes the degradation of short-lived proteins

AbstractThe fluorescent dye rhodamine 123 (R123) decreases the intracellular ATP levels and also inhibits the degradation of short-lived proteins in isolated hepatocytes. This inhibition affects lysosomal and, to some extent, non-lysosomal mechanisms. The degradation of short-lived proteins decreases more when ATP levels are less than 40% of those in control cells, in contrast to the reported linear correlation between ATP levels and degradation of long-lived proteins. R123 provides a powerful probe for clarifying the proteolytic mechanisms involved in degradation of short-lived proteins and the ATP requirements in protein degradation. Indeed, as illustrated, the results suggest different mechanisms for the degradation of short- and long-lived proteins. Moreover, they provide a warning for the clinical use of this reagent

Effects of concrete composition on transmission length of prestressing strands

The bond behaviour of prestressing strands in precast pretensioned concrete members, and its transmission length, depends on several factors. However, no consensus exists on the main parameters to be considered in the expressions to predict the transmission length. Usually when the concrete properties are considered, only the concrete compressive strength is included. This study analyzes the influence of concrete composition made up of different cement contents and water/cement ratios on the bond behaviour in transmission of seven-wire prestressing strands. The bond properties and the transmission lengths have been determined. The results show that the influence of the water/cement ratio is very small for concretes with lows cement contents, but the influence of the water/cement ratio on the transmission lengths is highly significant when cement content is high. The effect of cement content in the transmission lengths can reveal different tendencies based on the level of the water/cement ratioThe content of this article forms a part of research that the Universitat Politecnica de Valencia's Institute of Concrete Science and Technology (ICITECH) is presently conducting in conjunction with PREVALESA and ISOCRON. This study was funded by the Ministry of Education and Science and ERDF (Project BIA2006-05521). The authors wish to thank the above companies as well as the concrete structures laboratory technicians at the Universitat Politecnica de Valencia for their cooperation. Also, the authors wish to pay their respects to C.A. Arbelaez.Martí Vargas, JR.; Serna Ros, P.; Navarro Gregori, J.; Bonet Senach, JL. (2012). Effects of concrete composition on transmission length of prestressing strands. Construction and Building Materials. 27(1):350-356. https://doi.org/10.1016/j.conbuildmat.2011.07.038S35035627

Thermal performance of a solar hybrid dryer for Conilon coffee (Coffea canephora)

The study was aimed at design and development of an energy efficient hybrid solar dryer suitable for drying of organic Conilon coffee placed in the town of Seropédica, Rio de Janeiro, Brazil. The energy efficiency and the drying efficiency were the evaluation criteria for thermal performance of the hybrid solar dryer during the coffee drying. Temperature and relative humidity (RH) of the drying and ambient air, solar radiation intensity and coffee weight loss were monitored during the drying process. The process occurred over six consecutive days; the drying time was from 07:00 to 17:00 h, totalling 120 h of operation with an intermittent period (at night) of 14 h. During intermittence, the exhaust system kept off and solar collector and drying chamber sealed. The effective drying period took 60 h, with temperature and RH, respectively, of 38.3 °C and 60.6% outlet of the solar collector, 32.7 °C and 72.2% outlet drying chamber and 27.8 °C and 74.5% ambient air. The maximum temperature in the solar collector and drying chamber reached 54 and 47.7 °C, respectively, with an ambient air temperature of 32 °C at 12:00 h. These values showing temperature increase 22.2 °C in solar collector and 10 ºC drying chamber. The mean variation for the reduction in RH between the drying air inside the solar collector and the ambient air was 28%, while in the chamber obtained in a range of 10.5% at 13:00 h. The solar collector and dryer chamber efficiency were 29.1 and 40.8%, respectively, while the overall dryer efficiency 39.7%

Emissive Langmuir Probes in the Strong Emission Regime for the Determination of the Plasma Properties

The determination of the plasma potential Vpl of unmagnetized plasmas by using the floating potential of emissive Langmuir probes operated in the strong emission regime is investigated. The experiments evidence that, for most cases, the electron thermionic emission is orders of magnitude larger than the plasma thermal electron current. The temperature-dependent floating potentials of negatively biased Vpmenor queVpl emissive probes are in agreement with the predictions of a simple phenomenological model that considers, in addition to the plasma electrons, an ad-ditional electron group that contributes to the probe current. The latter would be constituted by a fraction of the repelled electron thermionic current, which might return back to the probe with a different energy spectrum. Its origin would be a plasma potential well formed in the plasma sheath around the probe, acting as a virtual cathode or by collisions and electron thermalization pro-cesses. These results suggest that, for probe bias voltages close to the plasma potential Vp?Vpl, two electron populations coexist, i.e., the electrons from the plasma with temperatureTeand a large group of returned thermionic electrons. These results question the theoretical possibility of measuring the electron temperature by using emissive probes biased to potentials Vp about lower equal than ?Vpl