Influence of the level of neurological deficit on the efficacy of cell therapy in a model of severe traumatic brain injury

Objetivo: Determinar si el nivel de déficit neurológico influye en la eficacia de la terapia celular con células madre mesenquimales (CMM) de la médula ósea en un modelo experimental crónico de lesión cerebral traumática. Material y métodos: Se sometió a ratas Wistar adultas a un modelo de lesión cerebral traumática. Dos meses después, se clasificaron en función de su nivel de déficit neurológico mediante dos tests funcionales: Escala de valoración sensitivo-motora y Tiempo de permanencia en la zona interior (Video-Tracking-Box test, VTB test). Se inyectó suero salino solo o CMM en suero salino en el tejido cerebral dañado de los animales que obtuvieron la clasificación neurológica de lesión moderada o grave según el nivel permanente de su déficit funcional. Todos los animales se evaluaron durante los dos meses siguientes para determinar la posible eficacia de la administración de las CMM. Al finalizar el estudio, los animales se eutanasiaron y se estudiaron sus cerebros. Resultados: Se constata una recuperación funcional significativa en los animales con lesión moderada que recibieron las CMM, pero no en los animales con lesión grave cuando se compara con los controles. Conclusiones: Las conclusiones obtenidas sugieren que la gravedad de la lesión neurológica puede influir en la potencial eficacia de la terapia celular cuando es aplicada en una lesión cerebral traumática crónicaObjective: To study if the level of neurological deficit influences the efficacy of cell therapy with bone marrow stromal cells (BMSC), in an experimental model of chronic traumatic brain injury (TBI). Material and methods: Adult Wistar rats were subjected to a model of traumatic brain injury. Two months later they were classified according to their level of neurological deficit. For that, two functional tests: Scale of sensory- motor assessment and time spent in the inner zone in the Video-Tracking-Box test, were used. Saline alone or BMSC in saline was injected into the damaged brain tissue of animals suffering a permanent level of functional deficit classified as moderate or severe. All experimental groups were evaluated during the next two months to determine the potential effectiveness of the intracerebral administration of BMSC. At the end of the study animals were euthanized and their brains were studied. Results: The results show a significant functional recovery in animals with moderate injury who received BMSC, but there was no significant recovery in animals with severe traumatic brain injury when compared with controls. Conclusion: The severity of neurologic injury may influence the potential efficacy of cell therapy applied to chronic TBIEsta investigación ha sido financiada por FUNDACIÓN MAPFR

Endogenous neurogenesis after traumatic brain injury and its modulation by cell therapy

Objetivo: Estudiar la neurogénesis inducida por una lesión traumática cerebral y su modulación mediante terapia celular. Material y métodos: Se realizó un modelo de lesión cerebral traumática en ratas Wistar adultas causando un daño cerebral grave. Transcurridos dos meses de la lesión, se administraron intralesionalmente células madre estromales (CME) alogénicas obtenidas de médula ósea, o suero fisiológico. Se estudió histológicamente la zona subventricular (ZSV) de cada animal al objeto de valorar la neurogénesis endógena espontánea tras la lesión y su posible modificación por la administración intracerebral de CME. Igualmente, se valoró la modificación de los déficit funcionales tras el tratamiento. Resultados: Se detectó un aumento en la neurogénesis endógena en el grupo tratado con CME respecto del control. Este hallazgo estuvo asociado a una progresiva mejoría en la respuesta motora y sensorial en el grupo de animales que recibieron CME comparado con el grupo de animales control. Conclusiones: La eficacia de la terapia celular para revertir los déficit funcionales secundarios a una lesión traumática cerebral parece correlacionarse con un incremento de la neurogénesis endógena a nivel de la SVZObjective: To study the neurogenesis induced by traumatic brain injury and its modulation by cell therapy. Material and methods: We performed a model of traumatic brain injury in adult Wistar rats, causing severe brain damage. After 2 months of injury, allogeneic bone marrow stromal cells (BMSC) or saline were administered intralesionally. We studied histologically the subventricular zone (SVZ) of each animal, in order to assess endogenous neurogenesis after traumatic brain injury and its possible modulation by intracerebral administration of BMSC. Furthermore, we studied the modification of neurological deficits after BMSC administration. Results: We detected a rise in endogenous neurogenesis in the group treated with BMSC with respect to the control. This finding was associated with a progressive improvement in motor and sensory response in the group of animals that received BMSC compared with control animals. Conclusion: The efficacy of cell therapy using BMSC appears to correlate with an increase of endogenous neurogenesis at SVZ levelEsta investigación ha sido financiada por FUNDACIÓN MAPFR

Relationship between olive oil consumption and ankle-brachial pressure index in a population at high cardiovascular risk

The aim of this study was to ascertain the association between the consumption of different categories of edible olive oils (virgin olive oils and olive oil) and olive pomace oil and ankle-brachial pressure index (ABI) in participants in the PREDIMED-Plus study, a trial of lifestyle modification for weight and cardiovascular event reduction in individuals with overweight/obesity harboring the metabolic syndrome. Methods: We performed a cross-sectional analysis of the PREDIMED-Plus trial. Consumption of any category of olive oil and olive pomace oil was assessed through a validated food-frequency questionnaire. Multivariable linear regression models were fitted to assess associations between olive oil consumption and ABI. Additionally, ABI ≤1 was considered as the outcome in logistic models with different categories of olive oil and olive pomace oil as exposure. Results: Among 4330 participants, the highest quintile of total olive oil consumption (sum of all categories of olive oil and olive pomace oil) was associated with higher mean values of ABI (beta coefficient: 0.014, 95% confidence interval [CI]: 0.002, 0.027) (p for trend = 0.010). Logistic models comparing the consumption of different categories of olive oils, olive pomace oil and ABI ≤1 values revealed an inverse association between virgin olive oils consumption and the likelihood of a low ABI (odds ratio [OR] 0.73, 95% CI [0.56, 0.97]), while consumption of olive pomace oil was positively associated with a low ABI (OR 1.22 95% CI [1.00, 1.48]). Conclusions: In a Mediterranean population at high cardiovascular risk, total olive oil consumption was associated with a higher mean ABI. These results suggest that olive oil consumption may be beneficial for peripheral artery disease prevention, but longitudinal studies are needed

Predictive Power of the "Trigger Tool" for the detection of adverse events in general surgery: a multicenter observational validation study

Background In spite of the global implementation of standardized surgical safety checklists and evidence-based practices, general surgery remains associated with a high residual risk of preventable perioperative complications and adverse events. This study was designed to validate the hypothesis that a new “Trigger Tool” represents a sensitive predictor of adverse events in general surgery. Methods An observational multicenter validation study was performed among 31 hospitals in Spain. The previously described “Trigger Tool” based on 40 specific triggers was applied to validate the predictive power of predicting adverse events in the perioperative care of surgical patients. A prediction model was used by means of a binary logistic regression analysis. Results The prevalence of adverse events among a total of 1,132 surgical cases included in this study was 31.53%. The “Trigger Tool” had a sensitivity and specificity of 86.27% and 79.55% respectively for predicting these adverse events. A total of 12 selected triggers of overall 40 triggers were identified for optimizing the predictive power of the “Trigger Tool”. Conclusions The “Trigger Tool” has a high predictive capacity for predicting adverse events in surgical procedures. We recommend a revision of the original 40 triggers to 12 selected triggers to optimize the predictive power of this tool, which will have to be validated in future studies

Psicothema

Resumen tomado de la publicaciónImpacto psicológico de la pandemia de la COVID-19 en España: un estudio longitudinal. Antecedentes: el objetivo es evaluar el impacto psicológico de la pandemia generada por la COVID-19 en la población general española longitudinalmente en cuatro momentos: tras dos semanas del inicio del confinamiento, al mes, a los dos meses y al año. Método: las evaluaciones se realizaron mediante una encuesta online, se siguió a una muestra de 3.480 personas en la primera recogida de datos y de 1.041, 569 y 550 personas en los sucesivos momentos de evaluación. Se evaluó la presencia de síntomas depresivos (PHQ-2), de ansiedad (GAD-2), de estrés postraumático (PCL-C-2), el apoyo social (EMAS), la soledad (UCLA-3) y la discriminación (InDI-D). Resultados: se han producido cambios significativos en las variables de depresión y ansiedad con una presencia mayor de dicha sintomatología al año (p < .01), así como en la variable de apoyo social, que muestra una reducción significativa un año después (p < .001), y en la discriminación interseccional, con una mayor discriminación (p < .001). Los modelos temporales no muestran diferencias significativas en cuanto a sintomatología postraumática (p = .12) ni soledad (p = .19). Conclusiones: la pandemia ha tenido un impacto negativo en la salud mental y estos efectos son todavía peores un año después.ES

Relationship between olive oil consumption and ankle-brachial pressure index in a population at high cardiovascular risk

Background and aims: The aim of this study was to ascertain the association between the consumption of different categories of edible olive oils (virgin olive oils and olive oil) and olive pomace oil and ankle-brachial pressure index (ABI) in participants in the PREDIMED-Plus study, a trial of lifestyle modification for weight and cardiovascular event reduction in individuals with overweight/obesity harboring the metabolic syndrome. Methods: We performed a cross-sectional analysis of the PREDIMED-Plus trial. Consumption of any category of olive oil and olive pomace oil was assessed through a validated food-frequency questionnaire. Multivariable linear regression models were fitted to assess associations between olive oil consumption and ABI. Additionally, ABI ≤1 was considered as the outcome in logistic models with different categories of olive oil and olive pomace oil as exposure. Results: Among 4330 participants, the highest quintile of total olive oil consumption (sum of all categories of olive oil and olive pomace oil) was associated with higher mean values of ABI (beta coefficient: 0.014, 95% confidence interval [CI]: 0.002, 0.027) (p for trend = 0.010). Logistic models comparing the consumption of different categories of olive oils, olive pomace oil and ABI ≤1 values revealed an inverse association between virgin olive oils consumption and the likelihood of a low ABI (odds ratio [OR] 0.73, 95% CI [0.56, 0.97]), while consumption of olive pomace oil was positively associated with a low ABI (OR 1.22 95% CI [1.00, 1.48]). Conclusions: In a Mediterranean population at high cardiovascular risk, total olive oil consumption was associated with a higher mean ABI. These results suggest that olive oil consumption may be beneficial for peripheral artery disease prevention, but longitudinal studies are needed

Relationship between olive oil consumption and ankle-brachial pressure index in a population at high cardiovascular risk

The aim of this study was to ascertain the association between the consumption of different categories of edible olive oils (virgin olive oils and olive oil) and olive pomace oil and ankle-brachial pressure index (ABI) in participants in the PREDIMED-Plus study, a trial of lifestyle modification for weight and cardiovascular event reduction in individuals with overweight/obesity harboring the metabolic syndrome. Methods: We performed a cross-sectional analysis of the PREDIMED-Plus trial. Consumption of any category of olive oil and olive pomace oil was assessed through a validated food-frequency questionnaire. Multivariable linear regression models were fitted to assess associations between olive oil consumption and ABI. Additionally, ABI ≤1 was considered as the outcome in logistic models with different categories of olive oil and olive pomace oil as exposure. Results: Among 4330 participants, the highest quintile of total olive oil consumption (sum of all categories of olive oil and olive pomace oil) was associated with higher mean values of ABI (beta coefficient: 0.014, 95% confidence interval [CI]: 0.002, 0.027) (p for trend = 0.010). Logistic models comparing the consumption of different categories of olive oils, olive pomace oil and ABI ≤1 values revealed an inverse association between virgin olive oils consumption and the likelihood of a low ABI (odds ratio [OR] 0.73, 95% CI [0.56, 0.97]), while consumption of olive pomace oil was positively associated with a low ABI (OR 1.22 95% CI [1.00, 1.48]). Conclusions: In a Mediterranean population at high cardiovascular risk, total olive oil consumption was associated with a higher mean ABI. These results suggest that olive oil consumption may be beneficial for peripheral artery disease prevention, but longitudinal studies are needed