Novel submerged photocatalytic membrane reactor for treatment of olive mill wastewaters

POCI-01-0145-FEDER-007265A new hybrid photocatalytic membrane reactor that can easily be scaled-up was designed, assembled and used to test photocatalytic membranes developed using the sol–gel technique. Extremely high removals of total suspended solids, chemical oxygen demand, total organic carbon, phenolic and volatile compounds were obtained when the hybrid photocatalytic membrane reactor was used to treat olive mill wastewaters. The submerged photocatalytic membrane reactor proposed and the modified membranes represent a step forward towards the development of new advanced treatment technology able to cope with several water and wastewater contaminants.publishersversionpublishe

Solvent-free process for the development of photocatalytic membranes

PTDC/EAM-AMB/30989/2017.This work described a new sustainable method for the fabrication of ceramic membranes with high photocatalytic activity, through a simple sol-gel route. The photocatalytic surfaces, prepared at low temperature and under solvent-free conditions, exhibited a narrow pore size distribution and homogeneity without cracks. These surfaces have shown a highly efficient and reproducible behavior for the degradation of methylene blue. Given their characterization results, the microfiltration photocatalytic membranes produced in this study using solvent-free conditions are expected to effectively retain microorganisms, such as bacteria and fungi that could then be inactivated by photocatalysis.publishe

Stability of polymeric membranes to UV exposure before and after coating with TiO2 nanoparticles

Acknowledgments: Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, Nº 2019/ 04319-9), Associate Laboratory for Green Chemistry—LAQV, Unidade de Tecnologia de Células Animais do iBET e à Paula Alves, iNOVA4Health—UIDB/Multi/04462/2020, a program financially supported by Fundação para a Ciência e Tecnologia/Ministério da Educação e Ciência, through national funds is acknowledged. Funding from INTERFACE Program, through the Innovation, Technology and Circular Economy Fund (FITEC), is also gratefully acknowledged. Funding Information: Funding: Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, Nº 2019/04319-9), Associate Laboratory for Green Chemistry—LAQV (through projects UIDB/50006/2020 and UIDP/ 50006/2020), Fundação para a Ciência e a Tecnologia through the project PTDC/EAM-AMB/30989/2017, Unidade de Tecnologia de Células Animais do iBET, iNOVA4Health—UIDB/Multi/04462/2020. Funding Information: Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo (FAPESP, N? 2019/04319-9), Associate Laboratory for Green Chemistry?LAQV (through projects UIDB/50006/2020 and UIDP/ 50006/2020), Funda??o para a Ci?ncia e a Tecnologia through the project PTDC/EAM-AMB/30989/2017, Unidade de Tecnologia de C?lulas Animais do iBET, iNOVA4Health?UIDB/Multi/04462/2020. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.The combination of photocatalysis and membrane filtration in a single reactor has been proposed, since the photocatalytic treatment may degrade the pollutants retained by the membrane and reduce fouling. However, polymeric membranes can be susceptible to degradation by UV radiation and free radicals. In the present study, five commercial polymeric membranes were exposed to ultraviolet (UV) radiation before and after applying a sol–gel coating with TiO2 nanoparticles. Membrane stability was characterized by changes in hydrophilicity as well as analysis of soluble substances and nanoparticles detached into the aqueous medium, and by Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), and energy-dispersive X-ray spectrometry (EDS) for structural, morphological, and elemental distribution analysis, respectively. The TiO2 coating conferred photocatalytic properties to the membranes and protected them during 6 h of UV radiation exposures, reducing or eliminating chemical and morphological changes, and in some cases, improving their mechanical resistance. A selected commercial nanofiltration membrane was coated with TiO2 and used in a hybrid reactor with a low-pressure UV lamp, promoting photocatalysis coupled with cross-flow filtration in order to remove 17α-ethinylestradiol spiked into an aqueous matrix, achieving an efficiency close to 100% after 180 min of combined filtration and photocatalysis, and almost 80% after 90 min.publishersversionpublishe

Reference values for lysosomal enzymes activities using dried blood spots samples - a Brazilian experience

Background: Lysosomal storage diseases (LSD) are inherited disorders caused by deficiency of lysosomal enzymes in which early diagnosis is essential to provide timely treatment. This study reports interval values for the activity of lysosomal enzymes that are deficient in Mucopolysaccharidosis type I, Fabry, Gaucher and Pompe disease, using dried blood spots on filter paper (DBS) samples in a Brazilian population.Results: Reference activity values were obtained from healthy volunteers samples for alpha-galactosidase A (4.57 +/- 1.37 umol/L/h), beta-glucosidase (3.06 +/- 0.99 umol/L/h), alpha-glucosidase (ratio: 13.19 +/- 4.26; % inhibition: 70.66 +/- 7.60), alpha-iduronidase (3.45 +/- 1.21 umol/L/h) and beta-galactosidase (14.09 +/- 4.36 umol/L/h).Conclusion: Reference values of five lysosomal enzymes were determined for a Brazilian population sample. However, as our results differ from other laboratories, it highlights the importance of establishing specific reference values for each center

Degradation of neonicotinoids and caffeine from surface water by photolysis

Funding Information: Publicly available datasets were analyzed in this study. This data can be obtained by contacting the corresponding author of the work at: [email protected] Acknowledgments: Financial support from Fundação para a Ciência e a Tecnologia through the project PTDC/EAM-AMB/30989/2017 is gratefully acknowledged. iNOVA4Health— UID/Multi/04462/2013, a program financially supported by Fundação para a Ciência e Tecnolo-gia/Ministério da Educação e Ciência through national funds and co-funded by FEDER under the PT2020 Partnership Agreement is gratefully acknowledged. This work is also supported by the Associate Laboratory for Green Chemistry—LAQV, which is financed by national funds from FCT/MCTES (UIDB/50006/2020). Funding from the INTERFACE Programme, through the Innovation, Technology and Circular Economy Fund (FITEC), is gratefully acknowledged.Along with rapid social development, the use of insecticides and caffeine-containing products increases, a trend that is also reflected in the composition of surface waters. This study is focused on the phototreatment of a surface water containing three neonicotinoids (imidacloprid, thi-amethoxam, and clothianidin) and caffeine. Firstly, the radiation absorption of the target pollutants and the effect of the water matrix components were evaluated. It was observed that the maximum absorption peaks appear at wavelengths ranging from 246 to 274 nm, and that the water matrix did not affect the efficiency of the removal of the target pollutants. It was found that the insecticides were efficiently removed after a very short exposure to UV irradiation, while the addition of hydrogen peroxide was needed for an efficient caffeine depletion. The electrical energy per order was estimated, being the lowest energy required (9.5 kWh m−3 order−1) for the depletion of thiamethoxan by indirect photolysis, and a concentration of hydrogen peroxide of 5 mg dm−3. Finally, a prelimi-nary evaluation on the formation of by-products reveals that these compounds play a key role in the evolution of the ecotoxicity of the samples, and that the application of direct photolysis reduces the concentration of these intermediates.publishersversionpublishe

The effect of legislation on the treatment practices and role of naturopaths in South Africa

In South Africa naturopaths have been practising for over half a century. Over this period, changes in legislation have resulted in different levels of training and registration processes - which has impacted on the profession in various ways. This paper explores the effect of legislation on the treatment practices and role of naturopaths in South Africa. Methods: This was a qualitative study which used an exploratory approach. Participants were sampled from the list of naturopaths registered with the Allied Health Professions Council of South Africa (AHPCSA). A set of 15 openended survey questions were emailed to 59 naturopaths. Twenty one naturopaths participated: 13 responded via email and eight were interviewed. Responses were coded and thematically analysed. Results: It was found that despite differences in training and years of practice experience, four core treatment practices of diet therapy, lifestyle medicine, supplementation and physical therapies were common to all participants with the older, more experienced naturopaths using a wider range of treatment practices

Computational Approaches Drive Developments in Immune-Oncology Therapies for PD-1/PD-L1 Immune Checkpoint Inhibitors

Funding Information: This research was funded by Fundação para a Ciência e Tecnologia (FCT) Portugal, grant number UIDB/50006/2020 (LAQV-REQUIMTE), UIDP/04378/2020 and UIDB/04378/2020 (UCIBIO) and LA/P/0140/2020 (i4HB), the European Commission GLYCOTwinning (GA 101079417), the EJPRD ProDGNE (EJPRD/0001/2020 EU 825575) and SI I&DT, DCMatters (AVISO Nº 17/SI/2019) REF 47212. F.P. gratefully acknowledges FCT for an Assistant Research Position (CEECIND/01649/2021). Publisher Copyright: © 2023 by the authors.Computational approaches in immune-oncology therapies focus on using data-driven methods to identify potential immune targets and develop novel drug candidates. In particular, the search for PD-1/PD-L1 immune checkpoint inhibitors (ICIs) has enlivened the field, leveraging the use of cheminformatics and bioinformatics tools to analyze large datasets of molecules, gene expression and protein–protein interactions. Up to now, there is still an unmet clinical need for improved ICIs and reliable predictive biomarkers. In this review, we highlight the computational methodologies applied to discovering and developing PD-1/PD-L1 ICIs for improved cancer immunotherapies with a greater focus in the last five years. The use of computer-aided drug design structure- and ligand-based virtual screening processes, molecular docking, homology modeling and molecular dynamics simulations methodologies essential for successful drug discovery campaigns focusing on antibodies, peptides or small-molecule ICIs are addressed. A list of recent databases and web tools used in the context of cancer and immunotherapy has been compilated and made available, namely regarding a general scope, cancer and immunology. In summary, computational approaches have become valuable tools for discovering and developing ICIs. Despite significant progress, there is still a need for improved ICIs and biomarkers, and recent databases and web tools have been compiled to aid in this pursuit.publishersversionpublishe

Occurrence, nanofiltration treatment and toxicity screening

Funding Information: This work was also funded by Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior ( FCT / MCTES , Portugal) through national funds to iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020) and the Associate Laboratory LS4FUTURE (LA/P/0087/2020). Teresa I.A. Gouveia and Vanessa Jorge Pereira would like to thank the Portuguese Foundation for Science and Technology ( FCT ) for Ph.D. ( SFRH / BD /147301/2019) and CEECIND/02919/2018 grants, respectively. Funding Information: This research was financially supported by: (i) Project POCI-01-0145-FEDER-031297 (CytoStraTech)—funded by FEDER funds through COMPETE2020—Programa Operacional Competitividade e Internacionalização (POCI) and by national funds (PIDDAC) through FCT /MCTES; (ii) NORTE-01-0145-FEDER-000069 (Healthy Waters) co-funded by European Regional Development Fund ( ERDF ), through North Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement; (iii) UIDB/04750/2020 (EPIUnit) and LA/P/0064/2020 (ITR), funded by national funds through the FCT - Foundation for Science and Technology, I.P.; (iv) LA/P/0045/2020 (ALiCE), Base Fundings UIDB/00511/2020 and UIDP/00511/2020 (LEPABE) and UIDB/50020/2020 and UIDP/50020/2020 (LSRE- LCM ), funded by national funds through FCT / MCTES (PIDDAC). Publisher Copyright: © 2023 The AuthorsAntineoplastic drugs are pharmaceuticals that have been raising concerns among the scientific community due to: (i) their increasing prescription in the fight against the disease of the twentieth century (cancer); (ii) their recalcitrance to conventional wastewater treatments; (iii) their poor environmental biodegradability; and (iv) their potential risk to any eukaryotic organism. This emerges the urgency in finding solutions to mitigate the entrance and accumulation of these hazardous chemicals in the environment. Advanced oxidation processes (AOPs) have been taken into consideration to improve the degradation of antineoplastic drugs in wastewater treatment plants (WWTPs), but the formation of by-products that are more toxic or exhibit a different toxicity profile than the parent drug is frequently reported. This work evaluates the performance of a nanofiltration pilot unit, equipped with a Desal 5DK membrane, in the treatment of real WWTP effluents contaminated (without spiking) with eleven pharmaceuticals, five of which were never studied before. Average removals of 68 ± 23% were achieved for the eleven compounds, with decreasing risks from feed to permeate for aquatic organisms from receiving waterbodies (with the exception of cyclophosphamide, for which a high risk was estimated in the permeate). Aditionally, no significative impact on the growth and germination of three different seeds (Lepidium sativum, Sinapis alba, and Sorghum saccharatum) were determined for permeate matrix in comparison to the control.publishersversionpublishe

Fullerenes In Asphaltenes And Other Carbonaceous Materials: Natural Constituents Or Laser Artifacts.

The presence of fullerenes as natural constituents of carbonaceous materials or their formation as laser artifacts during laser desorption ionization (LDI) mass spectrometry (MS) analysis is reinvestigated and reviewed. The results using asphaltene samples with varying composition as well as standard polycyclic aromatic hydrocarbons (PAH) and fullerene samples as models have demonstrated that indeed Cn ring fullerenes are not natural constituents but they are formed as common and often as predominant artifacts upon laser radiation, and a series of incorrect assignments based on LDI-MS data of several carbonaceous materials seems unfortunately to have been made. When the present results are evaluated also in the light of the vast literature on LDI-MS of carbonaceous materials, the formation of fullerene artifacts seems particularly common for LDI-MS analysis of asphaltenes and other carbonaceous samples with considerably high levels of PAH and varies according to the type of laser used, and the intensity of the laser beam.14

Chemical Composition and Fermentation Profile of Perennial Peanut and Marandu Grass Mixed Silages

Perennial peanut has high quality, evidenced by the improvement of animal production in grazing, due to good contents of crude protein and digestibility, which makes it one of the best alternatives for low cost feeding (Paganella and Valls 2002). Grass ensilage associated with legumes is considered an alternative to meet the protein demand of cattle in the livestock. However, due to limited information on the techniques of grass ensilage with tropical legumes, this research aimed to evaluate the chemical composition and the fermentation profile of perennial peanut and Marandu mixed grass silages, treated or not with bacterial inoculant