743 research outputs found

    Plants of the Colonet Region, Baja California, Mexico, and a Vegetation Map of Colonet Mesa

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    The Colonet region is located at the southern end of the California Floristic Province, in an area known to have the highest plant diversity in Baja California. A preliminary list of vouchered specimens is developed for the area, and a vegetation map for Colonet Mesa is presented. The Colonet region has at least 435 vascular plant taxa, of which 383 are native to Baja California, and 52 are endemic or nearly endemic. This list includes five local endemic taxa known only from the Colonet region, 18 taxa on the California Native Plant Society List 1B of taxa that are rare, threatened, or endangered in California and elsewhere, and three on the Mexican NOM 059 list of protected taxa. The Mexican federal government has proposed to build a major port and a new city at Colonet. To understand the potential impact of the port on the regional biodiversity, we examine three areas---a Footprint area which will presumably be highly affected by the port, the Mesa area which contains all the known vernal pools, and a Buffer area surrounding the other two areas. The large Buffer shows the greatest number of native and endemic taxa, followed by the Mesa. The Footprint has two species of high conservation concern that are not represented elsewhere in the study area. The vegetation map of Colonet Mesa confirms the presence of large vernal pools and shows extensive maritime chaparral, neither of which has been reported from elsewhere in northwest Baja California. This report documents the high plant biodiversity of the region and highlights the rare and unique species and vegetation types of Colonet Mesa

    Удосконалення технології варених ковбас з використанням горохової пасти

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    Використання білкових препаратів рослинногота тваринного походження при виготовленні м’ясопродуктів в теперішній час є дуже актуальним. Оскільки не один білок рослинного чи тваринного походження не відповідає повністю формулі збалансованого харчування, створити більш повноцінні продукти харчування можна завдяки комбінації білків, лімітованим по різним амінокислота

    Targeted disruption of the orphan receptor Gpr151 does not alter pain-related behaviour despite a strong induction in dorsal root ganglion expression in a model of neuropathic pain

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    BACKGROUND: Gpr151 is an orphan GPCR whose function is unknown. The restricted pattern of neuronal expression in the habenula, dorsal horn of the spinal cord and dorsal root ganglion plus homology with the galanin family of receptors imply a role in nociception. RESULTS: Real-time quantitative RT-PCR demonstrated a 49.9 ± 2.9 fold highly significant (P < 0.001) increase in Gpr151 mRNA expression in the dorsal root ganglion 7 days after the spared nerve injury model of neuropathic pain. Measures of acute, inflammatory and neuropathic pain behaviours were not significantly different using separate groups of Gpr151 loss-of-function mutant mice and wild-type controls. Galanin at concentrations between 100 nM and 10 μM did not induce calcium signalling responses in ND7/23 cells transfected with Gpr151. CONCLUSIONS: Our results indicate that despite the very large upregulation in the DRG after a nerve injury model of neuropathic pain, the Gpr151 orphan receptor does not appear to be involved in the modulation of pain-related behaviours. Further, galanin is unlikely to be an endogenous ligand for Gpr151

    Using the language laboratory to develop the listening ability of adult learners of English by means of practice in the perception of stress

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    This thesis is concerned with specific aspects of two general problems : firstly, the inability of teachers and students to utilize the potential of the language laboratory to maximum effect, and secondly, the inability of many learners of English to acquire a confident understanding of spoken English. The language laboratory was designed as a class teaching aid according to certain principles of language learning and its use is limited and defined by the facilities it provides and the conditions upon its use. Since supplementary use of the language laboratory may lead to under-exploitation of facilities, there needs to be investigation into areas of language training, suggested by current research in related fields, in which the language laboratory can play a fully integrated training role. Recent work on speech perception and child language development suggests that stress and rhythm, as prosodic features, are important perceptual factors in the rapid and efficient understanding of connected English speech, and, consequently, that an absence of accurate stress perception may reduce the listener's decoding ability. An attempt is made in this thesis to develop materials for training in stress perception, and to test techniques for their exploitation which are especially suited to use in the language laboratory. The thesis begins with a consideration of problems and aims, and than continues in Chapter Two with a review of the main issues regarding language laboratory use found in the literature. There then follows a discussion on the exploitation of specific facilities offered by the language laboratory, and the conditions upon their successful use. In Chapter Three, teacher and student use of the language laboratory is observed and assessed in live sessions in four language schools in Britain. The following two chapters, Chapter Four and Chapter Five, are concerned with the perception of stress and rhythm, and its role in understanding connected English speech. After a brief review of the literature and research on models of speech perception and understanding, and on child language development, in which their relevance to second language learning is discussed, recent research is presented, which indicates the importance of stress and rhythm in the accurate decoding of connected speech. Its bearing on second language training is considered, together with the problems of testing listening comprehension. There follow the reports of a series of experiments in which the ability of native speakers and learners of English to perceive and produce different spacing and pacing patterns of stress was tested, as well as the effect of varying the spacing and pacing of stress on the understanding of connected speech. The next two chapters, Chapter Six and Chapter Seven, link language laboratory use and stress perception in the design of a battery of materials, the purpose of which is to train learners in the perception of stress at word and utterance levels. The battery, in the form of a complete course, is then tested in order to assess its value in improving listening comprehension ability in learners of English, and the degree to which it exploits language laboratory facilities. The final chapter, Chapter Eight, attempts to relate the conclusions formed to the larger process of receptive and productive language development within the language laboratory context, and to the place of the stress perception materials in a complete language course

    The generation of knock-in mice expressing fluorescently tagged galanin receptors 1 and 2

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    The neuropeptide galanin has diverse roles in the central and peripheral nervous systems, by activating the G protein-coupled receptors Gal(1), Gal(2) and the less studied Gal(3) (GalR1–3 gene products). There is a wealth of data on expression of Gal(1–3) at the mRNA level, but not at the protein level due to the lack of specificity of currently available antibodies. Here we report the generation of knock-in mice expressing Gal(1) or Gal(2) receptor fluorescently tagged at the C-terminus with, respectively, mCherry or hrGFP (humanized Renilla green fluorescent protein). In dorsal root ganglia (DRG) neurons expressing the highest levels of Gal(1)-mCherry, localization to the somatic cell membrane was detected by live-cell fluorescence and immunohistochemistry, and that fluorescence decreased upon addition of galanin. In spinal cord, abundant Gal(1)-mCherry immunoreactive processes were detected in the superficial layers of the dorsal horn, and highly expressing intrinsic neurons of the lamina III/IV border showed both somatic cell membrane localization and outward transport of receptor from the cell body, detected as puncta within cell processes. In brain, high levels of Gal(1)-mCherry immunofluorescence were detected within thalamus, hypothalamus and amygdala, with a high density of nerve endings in the external zone of the median eminence, and regions with lesser immunoreactivity included the dorsal raphe nucleus. Gal(2)-hrGFP mRNA was detected in DRG, but live-cell fluorescence was at the limits of detection, drawing attention to both the much lower mRNA expression than to Gal(1) in mice and the previously unrecognized potential for translational control by upstream open reading frames (uORFs)

    Plant communities affect arbuscular mycorrhizal fungal diversity and community composition in grassland microcosms

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    The diversity of arbuscular mycorrhizal (AM) fungi was investigated in an unfertilized limestone grassland soil supporting different synthesized vascular plant assemblages that had developed for 3 yr. The experimental treatments comprised: bare soil; monocultures of the nonmycotrophic sedge Carex flacca; monocultures of the mycotrophic grass Festuca ovina; and a species-rich mixture of four forbs, four grasses and four sedges. The diversity of AM fungi was analysed in roots of Plantago lanceolata bioassay seedlings using terminal-restriction fragment length polymorphism (T-RFLP). The extent of AM colonization, shoot biomass and nitrogen and phosphorus concentrations were also measured. The AM diversity was affected significantly by the floristic composition of the microcosms and shoot phosphorus concentration was positively correlated with AM diversity. The diversity of AM fungi in P. lanceolata decreased in the order: bare soil > C. flacca > 12 species > F. ovina. The unexpectedly high diversity in the bare soil and sedge monoculture likely reflects differences in the modes of colonization and sources of inoculum in these treatments compared with the assemblages containing established AM-compatible plants
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