Antibacterial Effects of the Essential Oils of CommonlyConsumed Medicinal Herbs Using an In Vitro Model.

The chemical composition and antibacterial activity of essential oils from 10 commonly consumed herbs: Citrus aurantium, C. limon, Lavandula angustifolia, Matricaria chamomilla, Mentha piperita, M. spicata, Ocimum basilicum, Origanum vulgare, Thymus vulgaris and Salvia officinalis have been determined. The antibacterial activity of these oils and their main components; i.e. camphor, carvacrol, 1,8-cineole, linalool, linalyl acetate, limonene, menthol, a-pinene, b-pinene, and thymol were assayed against the human pathogenic bacteria Bacillus subtilis, Enterobacter cloacae, Escherichia coli O157:H7, Micrococcus flavus, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella enteritidis, S. epidermidis, S. typhimurium, and Staphylococcus aureus. The highest and broadest activity was shown by O. vulgare oil. Carvacrol had the highest antibacterial activity among the tested components

Timing of antiretroviral therapy in Cambodian hospital after diagnosis of tuberculosis: impact of revised WHO guidelines

OBJECTIVE: To determine if implementation of 2010 World Health Organization (WHO) guidelines on antiretroviral therapy (ART) initiation reduced delay from tuberculosis diagnosis to initiation of ART in a Cambodian urban hospital. METHODS: A retrospective cohort study was conducted in a nongovernmental hospital in Phnom Penh that followed new WHO guidelines in patients with human immunodeficiency virus (HIV) and tuberculosis. All ART-naïve, HIV-positive patients initiated on antituberculosis treatment over the 18 months before and after guideline implementation were included. A competing risk regression model was used. FINDINGS: After implementation of the 2010 WHO guidelines, 190 HIV-positive patients with tuberculosis were identified: 53% males; median age, 38 years; median baseline CD4+ T-lymphocyte (CD4+ cell) count, 43 cells/µL. Before implementation, 262 patients were identified; 56% males; median age, 36 years; median baseline CD4+ cell count, 59 cells/µL. With baseline CD4+ cell counts ≤ 50 cells/µL, median delay to ART declined from 5.8 weeks (interquartile range, IQR: 3.7–9.0) before to 3.0 weeks (IQR: 2.1–4.4) after implementation (P < 0.001); with baseline CD4+ cell counts > 50 cells/µL, delay dropped from 7.0 (IQR: 5.3–11.3) to 3.6 (IQR: 2.9–5.3) weeks (P < 0.001). The probability of ART initiation within 4 and 8 weeks after tuberculosis diagnosis rose from 23% and 65%, respectively, before implementation, to 62% and 90% after implementation. A non-significant increase in 6-month retention and antiretroviral substitution was seen after implementation. CONCLUSION: Implementation of 2010 WHO recommendations in a routine clinical setting shortens delay to ART. Larger studies with longer follow-up are needed to assess impact on patient outcomes

Efficacy of Convalescent Plasma in Relation to Dose of Ebola Virus Antibodies

International audienc

Ecohydrological characterization of the Nyando wetland, Lake Victoria, Kenya: A State of System (SoS) analysis

Lake Victoria floodplain wetlands have a complex hydrological setting characterized by transition from a terrestrial to an aquatic environment. A state-of-system (SoS) analysis was carried out in a papyrus dominated wetland in the Nyando River Delta, on the eastern shores of Lake Victoria, Kenya, to characterize and provide data for detailed ecohydrological studies. The objectives of the study were to: (1) determine the spatio- temporal changes in the wetland evolution and (2) analyze the main hydrological factors that have influenced wetland evolution. Multi-temporal dry-season Landsat MSS, Landsat TM and Landsat ETM+ imagery covering Nyando Wetland and its surrounding area were processed and analyzed to generate time series polygon and polyline maps of the wetland and river. Results show that the wetland increased in size from 5,925 ha in 1950 to 9,925 ha in 1973, and declined to 4,527 ha in 2008. In the last 60 years, Nyando River has migrated in a general eastward direction. Time series hydrological data (1950-2009) were statistically tested for homogeneity  using the Spearman’s rank test for linear trends, Pettit test and Standard Normal Homogeneity test (SNHT) for change point analysis, and split-record tests performed for variance (F-test) and mean (t-test). In addition, data were analyzed using descriptive statistics and frequency analyses. Statistical test results show that the hydrological data series were homogeneous. Results of change point analyses indicate that total annual rainfall in Nyando declined in 1979, while the mean annual discharge for Nyando River and Lake Victoria levels had significant upward shifts in 1961. The decadal mean discharges varied significantly over time and increased by 80% from 11.45 m3/s observed in the 1950-1961 subset, reducing by 11.4 and 21.9% in the next two decadal sub-sets, before rising by 35.0% in 1990s and dropping by 24.0% in the last decade. The decadal mean annual lake levels increased from 1134.0 to 1135.43 m in the 1951-1961 and 1962-1972 and remained above the longterm mean of 1135.0 m for 43 years since 1962 before dropping drastically by 1.4 m to an average of 1134 m/year in 2005-2009. The highest recorded lake level at Kisumu Station was 1136.2 m in 1964 after increasing by 2.5 m from 1961. Discharge data exhibit trimodal seasonal patterns, while the lake levels had two peaks. The lake levels are more sensitive to direct lake rainfall. Changes in the Nyando wetland area are linked to the seasonal and episodic flood and drought events coupled with anthropogenic activities (regulation of lake levels, modification of river including cut-off meanders, river training and construction of dykes, drainage of wetland for cultivation, settlement and livestock grazing, abstraction of water for irrigation). A combination of these hydrological and human factors is the main cause of the Nyando Wetland evolution. If the land use trend continues unabated, then the increase in papyrus losses will pose a big challenge to the ecological functioning of the wetland and its support to sustaining community livelihoods.Key words: Nyando Wetland, River, ecohydrology, Lake Victoria

Morphometric and mechanical characterization, insulin loading and in-vivo biocompatibility of chitosan particles aggregated scaffolds for tissue engineering

In tissue engineering, scaffolds development presents, among others, 3 key requirements: adequate morphological characteristics, mechanical performance and in-vivo biocompatibility. The aim of the present study was to evaluate chitosan-based scaffolds produced by particle aggregation in these key issues. Furthermore, chitosan scaffolds were loaded with insulin to promote chondrogenic differentiation. Micro-Computed Tomography (m-CT) was carried out for accurate morphometric characterization quantifying porosity, interconnectivity, particles and pores size that shown to be adequate. Dynamical Mechanical Analysis (DMA) showed that scaffolds are mechanically stable in wet state with a storage modulus of 4.21 1.04 MPa at 1Hz frequency. Insulin-loaded scaffolds were characterized and studied with a pre-chondrogenic cell line (ATDC-5). The in-vitro release was carried out mimicking cell culture conditions quantified by micro-BCA. [...]info:eu-repo/semantics/publishedVersio

Liposomal amphotericin B for visceral leishmaniasis in human immunodeficiency virus-coinfected patients: 2-year treatment outcomes in Bihar, India

Reports on treatment outcomes of visceral leishmaniasis (VL)-human immunodeficiency virus (HIV) coinfection in India are lacking. To our knowledge, none have studied the efficacy of liposomal amphotericin B in VL-HIV coinfection. We report the 2-year treatment outcomes of VL-HIV-coinfected patients treated with liposomal amphotericin B followed by combination antiretroviral treatment (cART) in Bihar, India