Cromoglycate and Nedocromil: Influence on Airway Reactivity

Although basic mechanisms of bronchial hyper-responsiveness (BHR) are still incompletely understood, inflammation of airways is likely to play a fundamental role in modulating BHR in patients with asthma. The involvement of several inflammatory cells (eosinophils, mast cells, lymphocytes, neutrophils, macrophages and platelets) and of bioactive mediators secreted by these cells in the pathogenesis of asthma is well documented. Sodium cromoglycate and nedocromil sodium are two pharmacological agents which have anti-allergic and anti-inflammatory properties. Their clinical effectiveness in mild to moderate asthma, and the capacity to reduce BHR under different natural and experimental conditions, make them valuable drugs for maintenance therapy in patients with asthma

Impact of environmental factors on stilbene biosynthesis

Stilbenes are a small family of polyphenolic secondary metabolites that can be found in several distantly related plant species. These compounds act as phytoalexins, playing a crucial role in plant defense against phytopathogens, as well as being involved in the adaptation of plants to abiotic environmental factors. Among stilbenes, trans-resveratrol is certainly the most popular and extensively studied for its health properties. In recent years, an increasing number of stilbene compounds were subjected to investigations concerning their bioactivity. This review presents the most updated knowledge of the stilbene biosynthetic pathway, also focusing on the role of several environmental factors in eliciting stilbenes biosynthesis. The effects of ultraviolet radiation, visible light, ultrasonication, mechanical stress, salt stress, drought, temperature, ozone, and biotic stress are reviewed in the context of enhancing stilbene biosynthesis, both in planta and in plant cell and organ cultures. This knowledge may shed some light on stilbene biological roles and represents a useful tool to increase the accumulation of these valuable compounds

Multivariate calibration approach for quantitative determination of cell-line cross contamination by intact cell mass spectrometry and artificial neural networks

Cross-contamination of eukaryotic cell lines used in biomedical research represents a highly relevant problem. Analysis of repetitive DNA sequences, such as Short Tandem Repeats (STR), or Simple Sequence Repeats (SSR), is a widely accepted, simple, and commercially available technique to authenticate cell lines. However, it provides only qualitative information that depends on the extent of reference databases for interpretation. In this work, we developed and validated a rapid and routinely applicable method for evaluation of cell culture cross-contamination levels based on mass spectrometric fingerprints of intact mammalian cells coupled with artificial neural networks (ANNs). We used human embryonic stem cells (hESCs) contaminated by either mouse embryonic stem cells (mESCs) or mouse embryonic fibroblasts (MEFs) as a model. We determined the contamination level using a mass spectra database of known calibration mixtures that served as training input for an ANN. The ANN was then capable of correct quantification of the level of contamination of hESCs by mESCs or MEFs. We demonstrate that MS analysis, when linked to proper mathematical instruments, is a tangible tool for unraveling and quantifying heterogeneity in cell cultures. The analysis is applicable in routine scenarios for cell authentication and/or cell phenotyping in general

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR): A critical overview on the most promising applications of molecular scissors in oral medicine

The scientific community is continuously working to translate the novel biomedical techniques into effective medical treatments. CRISPR-Cas9 system (Clustered Regularly Interspaced Short Palindromic Repeats-9), commonly known as the “molecular scissor”, represents a recently developed biotechnology able to improve the quality and the efficacy of traditional treatments, related to several human diseases, such as chronic diseases, neurodegenerative pathologies and, interestingly, oral diseases. Of course, dental medicine has notably increased the use of biotechnologies to ensure modern and conservative approaches: in this landscape, the use of CRISPR-Cas9 system may speed and personalize the traditional therapies, ensuring a good predictability of clinical results. The aim of this critical overview is to provide evidence on CRISPR efficacy, taking into specific account its applications in oral medicine

Pennsylvania Folklife Vol. 33, No. 1

• Ritual and Folklore in Pennsylvania\u27s Wyoming Region: Old to New World Wonder • Ordinary Architecture of the Pennsylvania Germans: The Turnpike Houses • Set thy House in Order : Inheritance Patterns of the Colonial Pennsylvania Germans • The Literature on Fences, Walls and Hedges as Cultural Landscape Features • Aldes un Neieshttps://digitalcommons.ursinus.edu/pafolklifemag/1101/thumbnail.jp

Origin, structure and geochemistry of a rock glacier near Don Juan Pond, Wright Valley, Antarctica

The South Fork of Wright Valley contains one of the largest rock glaciers in the McMurdo Dry Valleys, Antarctica, stretching 7 km from the eastern boundary of the Labyrinth and terminating at Don Juan Pond (DJP). Here, we use results from ground-penetrating radar (GPR), qualitative field observations, soil leaching analyses and X-ray diffraction analyses to investigate rock glacier development. The absence of significant clean ice in GPR data, paired with observations of talus and interstitial ice influx from the valley walls, support rock glacier formation via talus accumulation. A quartz-dominated subsurface composition and discontinuous, well-developed desert pavements suggest initial rock glacier formation occurred before the late Quaternary. Major ion data from soil leaching analyses show higher salt concentrations in the rock glacier and talus samples that are close to hypersaline DJP. These observations suggest that DJP acts as a local salt source to the rock glacier, as well as the surrounding talus slopes that host water track systems that deliver solutes back into the lake, suggesting a local feedback system. Finally, the lack of lacustrine sedimentation on the rock glacier is inconsistent with the advance of a glacially dammed lake into South Fork during the Last Glacial Maximum

Superconductivity of a striped phase at the atomic limit

Abstract The resonant amplification of the superconducting critical temperature, the isotope effect, the change of the chemical potential in a particular 2D striped phase formed by superconducting stripes of width L alternated by separating stripes of width W with a period l at the atomic limit is studied. The critical temperature shows a 'shape resonance' by tuning the p charge density where the chemical potential m is in the range E -m -E q " v , where E is the bottom of the nth n n 0 n superlattice subband for n ) 2, and " v is the energy cutoff for the pairing interaction. The maximum critical 0 superconducting temperature is reached at the cross-over from 2D to 1D behavior. The particular properties of this electronic phase and its similarities with the normal and superconducting properties of doped cuprate perovskites are discussed. q 1998 Elsevier Science B.V