Contaminação de Uniformes Privativos Utilizados por Profissionais que Atuam nas Unidades de Terapia Intensiva

Background and Objectives: The study aimed to verify the presence of pathogens in private uniform professionals with access to the Intensive Care Unit (ICU) and analyze the sensitivity profile of the samples positive for Staphylococcus aureus. Methods: Samples were collected before and after the work day and later performed microbiological analyzes. Results: There was a percentage change in the total growth of 154%, with a predominance of coagulase-negative Staphylococcus (72.8%) followed by Staphylococcus aureus (38.4%). The latter showed considerable resistance to some specific antibiotic used for treatment. Conclusions: In this study it was possible to identify the presence of various potentially pathogenic micro-organisms in private uniform used by professionals in the ICU, verifying that the colonization of these bacteria has a progressive increase over the use of time. Strains of Staphylococcus aureus tested against the antimicrobial had a high profile susceptibility to drugs such as vancomycin and medium to oxacillin. With the large number of infections caused by bacteria resistant to different antibiotics, one should not rule out any possible means of dissemination of these microorganisms. However, more studies should be developed so that there is a confirmation that the bacteria found on the uniforms are the same that cause IRHs. KEYWORDS: Contamination. Protective clothing. Cross Infection. Intensive care unit.Antecedentes y objetivos: Los uniformes utilizados para acceder a Unidades de Terapia Intensiva (UTI) pueden contener bacterias resistentes a diferentes drogas. Por ello, resulta sumamente importante verificar si los mismos acarrean microorganismos potencialmente patogénicos para los pacientes y demás profesionales actuantes en tales unidades. El objetivo del estudio consistió en identificar, mediante análisis microbioló- gico, los microorganismos presentes en los uniformes individuales de profesionales actuantes en UTI, realizar un análisis comparativo de crecimiento bacteriano en las muestras recolectadas al inicio y final del trabajo y verificar el perfil de sensibilidad de las muestras positivas a Staphylococcus aureus. Métodos: Estudio transversal realizado a partir de recolección de muestras de los uniformes utilizados por profesionales que se desempeñan en las tres UTI de un hospital público de gran envergadura. Las recolecciones fueron efectuadas antes y después de la utilización de los uniformes en el ámbito laboral. Fueron realizados cultivo y test de sensibilidad a antimicrobianos. Resultados: Luego de los análisis microbiológicos fue posible observar un aumento del número de colonias del 154% al comparar las muestras finales con las iniciales. Entre los organismos encontrados, predominó el Staphylococcus coagulasa negativo (72,8%), seguido del Staphylococcus aureus (38,4%). Estos últimos mostraron sensibilidad a oxacilina del 56% en la UTI adultos, 80% en la UTI neonatal y 71% en la UTI pediátrica. El 100% de las muestras testeadas resultó sensible a vancomicina. Conclusión: Los uniformes individuales contenían un número significativo de microorganismos, pudiendo estos constituirse en posible vehículo para contaminaciones cruzadas. Palabras Clave: Contaminação. Roupa de Proteção. Infecção Hospitalar. Unidade de Terapia Intensiva.Os uniformes utilizados para o acesso as Unidades de Terapia Intensiva (UTI) podem conter bactérias resistentes a diferentes drogas, devido isso é de grande importância verificar se estes estão carreando micro-organismos possivelmente patogênicos aos pacientes e demais profissionais que trabalham nestas unidades. O estudo objetivou identificar, por meio da análise microbiológica, todos os micro-organismos presentes nos uniformes privativos de profissionais que atuam nas UTI, realizar uma análise comparativa de crescimento bacteriano nas amostras coletadas no início e fim de trabalho e verificar o perfil de sensibilidade das amostras positivas para Staphylococcus aureus. Foram coletadas amostras antes e após a jornada de trabalho, depois das análises microbiológicas foi possível observar um aumento no número de colônias de 154% quando comparada a colonização das amostras iniciais com as amostras finais, dentre os micro-organismos encontrados, predominou os Staphylococcus coagulase negativo (72,8%) seguido por Staphylococcus aureus (38,4%). Estes últimos apresentaram considerável resistência a alguns antimicrobianos específicos utilizados para tratamento. Concluiu-se que os uniformes privativos continham um número significativo de micro-organismos, podendo estes consistirem um possível veículo para contaminações cruzadas. DESCRITORES: Contaminação. Roupa de Proteção. Infecção Hospitalar. Unidade de Terapia Intensiva

NEOTROPICAL CARNIVORES: a data set on carnivore distribution in the Neotropics

Mammalian carnivores are considered a key group in maintaining ecological health and can indicate potential ecological integrity in landscapes where they occur. Carnivores also hold high conservation value and their habitat requirements can guide management and conservation plans. The order Carnivora has 84 species from 8 families in the Neotropical region: Canidae; Felidae; Mephitidae; Mustelidae; Otariidae; Phocidae; Procyonidae; and Ursidae. Herein, we include published and unpublished data on native terrestrial Neotropical carnivores (Canidae; Felidae; Mephitidae; Mustelidae; Procyonidae; and Ursidae). NEOTROPICAL CARNIVORES is a publicly available data set that includes 99,605 data entries from 35,511 unique georeferenced coordinates. Detection/non-detection and quantitative data were obtained from 1818 to 2018 by researchers, governmental agencies, non-governmental organizations, and private consultants. Data were collected using several methods including camera trapping, museum collections, roadkill, line transect, and opportunistic records. Literature (peer-reviewed and grey literature) from Portuguese, Spanish and English were incorporated in this compilation. Most of the data set consists of detection data entries (n = 79,343; 79.7%) but also includes non-detection data (n = 20,262; 20.3%). Of those, 43.3% also include count data (n = 43,151). The information available in NEOTROPICAL CARNIVORES will contribute to macroecological, ecological, and conservation questions in multiple spatio-temporal perspectives. As carnivores play key roles in trophic interactions, a better understanding of their distribution and habitat requirements are essential to establish conservation management plans and safeguard the future ecological health of Neotropical ecosystems. Our data paper, combined with other large-scale data sets, has great potential to clarify species distribution and related ecological processes within the Neotropics. There are no copyright restrictions and no restriction for using data from this data paper, as long as the data paper is cited as the source of the information used. We also request that users inform us of how they intend to use the data