Níveis de inclusão e tempo de exposição da cana-de-açúcar ao óxido de cálcio sobre parâmetros digestivos e o desempenho de novilhas Nelore

Avaliou-se nesta pesquisa o efeito da inclusão de óxido de cálcio (cal) e dos tempos de exposição da cana-de-açúcar à cal sobre o desempenho de novilhas Nelore, a digestibilidade dos componentes da dieta e a síntese de proteína microbiana ruminal (PBMic) e comparou-se o dióxido de titânio ao óxido crômico para estimar o consumo individual de concentrado. Utilizaram-se 30 fêmeas Nelore com aproximadamente 24 meses de idade e pesos médios de 285 ± 31 kg, alojadas em seis baias coletivas. As dietas foram arranjadas em esquema fatorial 3 × 2, com três níveis de cal (0; 0,5 ou 1% na matéria natural) e dois tempos de exposição (zero e três dias). Os tempos de exposição da cana-de-açúcar à cal não tiveram efeitos sobre as quantidades de nitrogênio microbiano, os consumos de matéria seca (MS) e orgânica (MO), proteína bruta (PB), total de nutrientes digestíveis e o ganho médio diário, porém influenciaram os consumos de extrato etéreo (EE), fibra em detergente neutro corrigida para cinzas e PB (FDNcp) e carboidratos não-fibrosos (CNF). Os níveis de cal influenciaram de forma linear decrescente os consumos de MO, FDNcp, CNF e NDT, e o GMD. Todavia, não foram observados efeitos dos indicadores (dióxido de titânio e óxido crômico) nem interações do tempo de exposição e do nível de cal sobre os consumos de MS, MO, PB, EE, FDNcp, CNF e NDT. Interações significativas do tempo de exposição e do nível de inclusão de cal foram observadas para as digestibilidades aparentes totais da MS, MO, PB e FDN e para o teor de NDT. Os indicadores dióxido de titânio e óxido crômico são igualmente efetivos para estimar os consumos individuais de concentrado de bovinos alimentados em grupo. O fornecimento de cana-de-açúcar armazenada durante três dias sem cal não altera o desempenho de novilhas em crescimento.It was evaluated in this work the effects of levels of inclusion and exposure times of sugarcane to calcium oxide on the performance of Nellore heifers, digestibility of dietary components and synthesis of ruminal microbial protein and it was compared titanium dioxide to chromium oxide to estimate the individual intake of concentrate. It was used 30 Nellore females at approximately 24 months of age and average body weight of 285 ± 31 kg and age of 24 months housed in six pens (50 m² approximately), each one of them with five animals (feeding group). The diets were allocated in a 3 × 2 factorial arrangement, containing three levels of calcium oxide (0; 0.5 and 1.0% of sugarcane fresh matter) and two levels of exposure time (0 and 3 days). There were no effects of calcium oxide exposure time on the intake of microbial nitrogen, dry matter (DM), organic matter (OM), crude protein (CP) and total digestible nutrients (TDN) and daily weight gain, but, it affected the intake of ether extract (EE), neutral detergent fiber corrected for ash and CP contamination (NDFcp), and nonfiber carbohydrate (NFC). The levels of whitewash decreased linearly the intake of OM, NDFap, NFC, and ADG. However, it was not observed effects of the markers (titanium dioxide and chromic oxide) nor interactions among exposure time and level of whitewash on intakes of DM, CP, EE, NDFap, NFC and TDN. Significant interactions between exposure time and level of inclusion of whitewash were observed for total aparent digestibility of DM, OM, and NDF and for content of TDN. Titanium oxide and chromic oxide are equally effective for estimating individual intakes of concentrate by bovine fed in group. Supply of sugar cane stored for three days without whitewash does not change performance of growing heifers

Beef quality traits of Nellore, F1 Simmental × Nellore and F1 Angus × Nellore steers fed at the maintenance level or ad libitum with two concentrate levels in the diet

This trial was conducted to evaluate some beef quality attributes of Nellore, F1 Simmental × Nellore and F1 Angus × Nellore steers finished on feedlot. The effects of feeding regime and genetic group on shear force, thawing losses, cooking (leak + evaporation) losses, total losses and muscle fiber type, as well as carcass pH and temperature during 24 h of chilling were evaluated. There was a genetic group effect on shear force, where the beef from F1 Simmental × Nellore and F1 Angus × Nellore animals had lower values than Nellore animals. Beef of the animals fed the diets with 1% and 2% of body weight on concentrated lost more liquid than the meat of the animals fed at maintenance during thawing and when considering total losses. During cooking there was a difference among the feeding regimes for drip losses which were greater on the animals fed the diet of 1% of body weight on concentrate, followed by the 2% diet and, finally, by the animals fed at maintenance. The muscle of the Nellore steers had larger proportion of intermediate fibers and lower proportion of oxidative fibers than the crossbred animals. The proportion of glycolytic fibers was not influenced by genetic group. The Nellore animals had larger proportion of fibers of fast contraction and smaller proportion of fibers of slow contraction when compared with the crossbred animals. Feeding regime did not influence the proportion of muscular fibers or shear force. Nellore cattle produce tougher beef than crossbred Simmental × Nellore or Angus × Nellore, although all of them have the potential to produce an acceptable beef when slaughtered at young age. Feed restriction up to 90 days is not enough to cause modification on muscle fiber frequencies, then not affecting beef quality

Seleção de progênies de nabo-forrageiro para germinação sob altas temperaturas

Due to the expansion of the wild crop to regions with higher temperatures is important to develop cultivars adapted to high heat. The aim was to select tests for evaluating seed physiological quality of wild radish to estimating genetic characters in order to select cultivars adapted to high temperature conditions. Hundred of half-brothers of wild radish were subjected to germination test and vigor (first count of germination, classification of seedling vigor, accelerated aging test, germination and testing of the first count at high temperature as well as seedling emergence in field. The germination test, first count test, accelerated aging and high germination test (20-35°C) can be used for the selection of wild radish crop populations adapted to germination and field emergence under high temperatures.Devido à expansão da cultura do nabo-forrageiro para regiões com temperaturas mais elevadas, torna-se de importância o desenvolvimento de cultivares adaptadas a altas temperaturas. Neste trabalho, o objetivo foi selecionar testes de avaliação da qualidade fisiológica de sementes nabo-forrageiro que estimam caracteres genéticos, visando a selecionar genótipos adaptados a condições de alta temperatura. Para isso, sementes de 100 progênies de meio-irmãos de nabo-forrageiro foram submetidas a teste de germinação e vigor (primeira contagem de germinação, classificação do vigor de plântulas, teste de envelhecimento acelerado, germinação e teste da primeira contagem a altas temperaturas), bem como emergência de plântulas em campo. O teste de primeira contagem, germinação, envelhecimento acelerado, primeira contagem e germinação em alta temperatura (20-35°C) são recomendados para delinear estratégias de seleção de populações de nabo-forrageiro adaptados à germinação e emergência de plântulas em campo, em condições de altas temperaturas.Universidade Federal de Goiás (UFG)Universidade Estadual Paulista (UNESP) Faculdade de Ciências Agrárias e Veterinárias (FCAV) Departamento de Produção VegetalUNESP Departamento de Produção VegetalUniversidade Estadual Paulista (UNESP) Faculdade de Ciências Agrárias e Veterinárias (FCAV) Departamento de Produção VegetalUNESP Departamento de Produção Vegeta

Punica granatum L. protects mice against hexavalent chromium-induced genotoxicity

This study investigated the chemoprotective effects of Punica granatum L. (Punicaceae) fruits alcoholic extract (PGE) on mice exposed to hexavalent chromium [Cr(VI)]. Animals were pretreated with PGE (25, 50 or 75 mg/kg/day) for 10 days and subsequently exposed to a sub-lethal dose of Cr(VI) (30 mg/kg). The frequency of micronucleated polychromatic erythrocytes in the bone marrow was investigated and the Cr(VI) levels were measured in the kidneys, liver and plasm. For the survival analysis, mice were previously treated with PGE for 10 days and exposed to a single lethal dose of Cr(VI) (50 mg/kg). Exposure to a sub-lethal dose of Cr(VI) induced a significant increase in the frequency of micronucleated cells. However, the prophylactic treatment with PGE led to a reduction of 44.5% (25 mg/kg), 86.3% (50 mg/kg) and 64.2% (75 mg/kg) in the incidence of micronuclei. In addition, the 50 mg/kg dose of PGE produced a higher chemoprotective effect, since the survival rate was 90%, when compared to that of the non-treated group. In these animals, reduced amounts of chromium were detected in the biological materials, in comparison with the other groups. Taken together, the results demonstrated that PGE exerts a protective effect against Cr(VI)-induced genotoxicity