Variability of Fragments of Nuclear Brca1 Gene, Exon 11, and Mitochondrial Cox1 Gene in House Mice Mus musculus

To clarify genetic differences between subspecies of the house mouse Mus musculus, their distribution, and hybridization, we first conducted a comparative analysis of variability of nucleotide sequences of fragments of the nuclear gene Brca1, exon 11 (2331 bp), and mitochondrial gene Cox1 (1260 bp) in 40 house mice from West and East Europe, Transcaucasia, Siberia, and Central and South Asia. Brca1 genotypes were divided into five main groups, which differed in a number of fixed substitutions. Genotypes of each group are characteristic for the certain geographical region and the following subspecies: M. m. musculus, M. m. domesticus, M. m. castaneus, and M. m. wagneri together with M. m. gansuensis; a fifth group corresponds to an unidentified subspecies or a distinct genetic form of M. musculus from India (Sikkim State). Besides the homozygous specimens, we revealed mice, which were heterozygous for all diagnostic loci simultaneously; these specimens were determined as hybrid. Hybrid mice were mainly found in the zones of contact of subspecies, but in some cases, quite far from one of the parent subspecies (possibly, due to transportation). In two hybrid mice (from Bakhtiari Province of Iran and Transbaikalia of Russia), unique Brca1 haplotypes were detected. It cannot be ruled out that, at least partly, they may be characteristic of the M. m. bactrianus and M. m. gansuensis subspecies, respectively. Thus, the results of the study showed that the nuclear Brca1 gene is a promising molecular genetic marker for the analysis of variability, differentiation, and hybridization of house mice as well for subspecific identification of M. musculus specimens. Despite more rapid evolution of the Cox1 gene, it is not well suited for discrimination of M.m. musculus, M. m. wagneri, M. m. gansuensis specimens and Transcaucasian representatives of M.m. domesticus due to introgression and long-term maintenance of foreign mitochondrial DNA in populations. However, Cox1 gene analysis (along with the diagnostics of animals by nuclear DNA) may be useful for estimation of population differences in M. m. castaneus and M.m. domesticus subspecies

Participation of VNIIFTRI in international comparisons in thefield of physical-chemical measurements

This paper considers the participation of FSUE «VNIIFTRI» in international comparisons: in the field of physicalchemical measurements, particularly in pH-metry and ionometry (pX); in the sphere of measuring inorganic components in various matrices. Measurement and calibration capabilities are presented – CMC lines in the BIPM database

Epizooty of Tularemia, Detected in the Population of the Common Vole in the Natural Focus of Steppe Type in the South-East of the Rostov Region in 2020

The aim of study was to conduct epizootiological monitoring of natural tularemia foci of the steppe type and investigate epizootic activity in the south-east of the Rostov Region.Materials and methods. An epizootiological survey was carried out on the territory of Remontnensky, Sal’sky and Peschanokopsky districts of the Rostov Region in 2019–2021. To capture and collect mammals, Ixodidae ticks and to study the samples of field material conventional methods were used.Results and discussion. Habitation of 16 species of small mammals, 6 species of Ixodidae ticks has been found. Molecular-genetic analysis of the voles has revealed the presence of the species Microtus arvalis obscurus in the studied area of the region. The circulation of the tularemia agent has been established in the population of common and social voles, forest mouse, hare, rook, Dermacentor marginatus, Hyalomma marginatum, removed from rooks. In May 2020, a high increase in the number of the social vole in the Remontnensky district (up to 21 %), in July 2020 – the common vole in the agrocenoses of the Sal’sky district (up to 33 %) was observed. An extensive epizooty of tularemia was detected in the population of common vole in the south-east of the Rostov Region and in adjacent territories in the Republic of Kalmykia and the Stavropol territory. Two cultures of the pathogen were isolated from the fallen and captured social voles, and four cultures – from the common vole. The isolated strains belong to the Holarctic subspecies of Francisella tularensis EryR. The results obtained attest to the activation of the natural tularemia focus in the south-east of the Rostov Region and its high epizootic activity

Участие ФГУП «ВНИИФТРИ» в международных сличениях в области физико-химических измерений

This paper considers the participation of FSUE «VNIIFTRI» in international comparisons: in the field of physicalchemical measurements, particularly in pH-metry and ionometry (pX); in the sphere of measuring inorganic components in various matrices. Measurement and calibration capabilities are presented – CMC lines in the BIPM database.В статье рассмотрено участие ФГУП «ВНИИФТРИ» в международных сличениях в области физико-химических измерений (рН-метрия и ионометрия (рХ)), а также в области измерений неорганических компонентов в различных матрицах, представлены измерительные и калибровочные возможности – СМС-строки в базе данных МБМВ

Generation of subnanometric platinum with high stability during transformation of a 2D zeolite into 3D

[EN] Single metal atoms and metal clusters have attracted much attention thanks to their advantageous capabilities as heterogeneous catalysts. However, the generation of stable single atoms and clusters on a solid support is still challenging. Herein, we report a new strategy for the generation of single Pt atoms and Pt clusters with exceptionally high thermal stability, formed within purely siliceous MCM-22 during the growth of a two-dimensional zeolite into three dimensions. These subnanometric Pt species are stabilized by MCM-22, even after treatment in air up to 540 degrees C. Furthermore, these stable Pt species confined within internal framework cavities show size-selective catalysis for the hydrogenation of alkenes. High-temperature oxidation-reduction treatments result in the growth of encapsulated Pt species to small nanoparticles in the approximate size range of 1 to 2 nm. The stability and catalytic activity of encapsulated Pt species is also reflected in the dehydrogenation of propane to propylene.This work was funded by the Spanish Government (Consolider Ingenio 2010-MULTICAT (CSD2009-00050) and MAT2014-52085-C2-1-P) and by the Generalitat Valenciana (Prometeo). The Severo Ochoa Program (SEV-2012-0267) is gratefully acknowledged. L.L. thanks ITQ for a contract. The authors also thank the Microscopy Service of UPV for the TEM and STEM measurements. The HAADF-HRSTEM works were conducted in the Laboratorio de Microscopias Avanzadas (LMA) at the Instituto de Nanociencia de Aragon (INA)-Universidad de Zaragoza (Spain), a Spanish ICTS National Facility. Some of the research leading to these results has received funding from the European Union Seventh Framework Program under Grant Agreement 312483-ESTEEM2 (Integrated Infrastructure Initiative-I3). R.A. also acknowledges funding from the Spanish Ministerio de Economia y Competitividad (FIS2013-46159-C3-3-P) and the European Union Horizon 2020 research and innovation programme under the Marie Sldodowska-Curie grant agreement No. 642742.Liu, L.; Díaz Morales, UM.; Arenal, R.; Agostini, G.; Concepción Heydorn, P.; Corma Canós, A. (2017). Generation of subnanometric platinum with high stability during transformation of a 2D zeolite into 3D. Nature Materials. 16(1):132-138. https://doi.org/10.1038/NMAT4757S132138161Boronat, M., Leyva-Perez, A. & Corma, A. Theoretical and experimental insights into the origin of the catalytic activity of subnanometric gold clusters: attempts to predict reactivity with clusters and nanoparticles of gold. Acc. Chem. Res. 47, 834–844 (2014).Flytzani-Stephanopoulos, M. & Gates, B. C. Atomically dispersed supported metal catalysts. Ann. Rev. Chem. Bio. Eng. 3, 545–574 (2012).Gates, B. C. Supported metal clusters: synthesis, structure, and catalysis. Chem. 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CURRENT STATUS OF POPULATION AND ECOLOGICAL PECULIARITIES OF THE EURASIAN BEAVER CASTOR FIBER L. IN ROSTOV REGION

Aim. Are considered the issues of distribution, the current state, and some ecological peculiarities of the Eurasian beaver Castor fiber in the Rostov region in the south of their range. Methods. The materials presented in the study were collected predominantly in the spring-summer months, based on the information from hunting specialists. Was used the method of counting the number of inhabited settlements. Was also considered the confinement of the animal to certain stations, as well as to the width of the river bed. Results. Over the past two decades, this species has significantly expanded its distribution in the region and settled along the right bank of the Don River up to its mouth. Currently, the number of beavers in the Rostov region is estimated at over 2300 individuals. At the same time, the overwhelming majority of them inhabit the administrative regions located in the north of the region and are more connected with the rivers of Seversky Donets, Kalitva, Chir, the middle course of the Don River. Conclusion. In the Rostov region, the beaver demonstrates the main elements of occupying the territories, which is expressed in the prevalence of construction of burrows over the construction of huts, as well as the absence of dams and canals. In the near future, there is a probable increase in the density of the population of the beaver in the lower reaches of the Don, as well as the colonization of the rivers of the northern part of the Azov Sea

Lederutdanning innen helse- og omsorgssektoren

Behovet for kompetente ledere i helse- og omsorgstjenesten i kommunene er stort, det samme gjelder den fylkeskommunale tannhelsetjenesten. Gjennom arbeidsseminarer og kvalitative intervjuer med kommunalsjefer og enhetsledere har vi kartlagt det eksisterende utdanningstilbudet og behovet for lederutdanning med vekt på tema og organisering

Influence of modifying additives on formation of supported copper nanoparticles

The influence of modifying additives of Ce, Zr, La and Cs oxides on changes of electronic state of supported copper during the catalytic reaction of butane complete oxidation has been studied by the methods of IR-spectroscopy of adsorbed CO, XPS, EXAFS and XRD. The modifying additions of cerium and zirconium oxides stabilize the ionic state of copper, while lanthanum and cesium oxides decrease the effective charge of copper ions. The observed effects are caused by variation in metal dispersivity in the modified samples and by electron donor-acceptor interaction of the surface atoms and ions of copper with the modifier