Geoheritage of Golija and Pešter

Sprovedena istraživanja u ovoj studiji obuhvatila su izdvajanje, opis, evaluaciju i valorizaciju objekata geonasleđa u cilju razvoja istraživane oblasti sa stanovišta geonauka, geoturizma i geoekologije. Na izučavanom području objekti geonasleđa su evidentirani, interpretirani, vrednovani i kategorisani, dat je predlog promocije geoobjekata, i na kraju predložena dopuna inventara objekata geonasleđa Srbije. U protekle četiri godine, na kompleksu geotopa Golije i Peštera istražena su geološka svojstva stena (tip stene, starost, fosilni sadržaj, morfološke i hidrogeološke karakteristike itd.), na osnovu čega je bilo omogućeno sistematsko i kompleksno rangiranje objekata u smislu geonasleđa. Izdvojeno je i predloženo 13 potencijalnih objekata geonasleđa (1 objekat lokalnog značaja, 8 objekata regionalnog značaja i 3 objekta nacionalnog značaja). Na Inventar listi geonasleđa Srbije nalazi se 12 objekata geonasleđa sa ovog područja, koji su svrstani u 3 kategorije (7 objekata regionalnog značaja, 4 objekta nacionalnog značaja i 1 objekat međunarodnog značaja). Osnovni motiv za izradu ove doktorske disertacije je da se Golija - ekološki krov Srbije i Pešter - najveća visoravan na Balkanu, sa izuzetnim prirodnim vrednostim,a kao i kulturno - istorijskim, predstavi široj stručnoj, kulturnoj i turističkoj javnosti kao veliki prirodni, ekološko-turistički i privredni potencijal, ne samo nacionalnog, već i međunarodnog značaja.Investigations carried out during production of this thesis included selection, description, evaluation and valorization of geosites in order to support development of the studied area in the fields of geosciences, geotourism and geoecology. The geosites were registered, interpreted, evaluated and categorized, recommendations about promotion of the geosites were given and, finally, suggestions about updating the inventory of Serbian geoheritage sites were made. Geological properties (rock type, age, fossil content, morphological and hydrogeological characteristics, etc.) of rocksat different localities on Golija Mt. and Pešter plateau were studied during the last four years, which enabled systematic and complex classification of geosites in terms of geoheritage. Thirteen potential geosites were selected (1 geosite of local importance, 8 geosites of regional importance and 3 geosites of national importance). The Inventory of Serbian geoheritage sites already includes 12 geosites from this area, which are classified into 3 categories (7 geosites of regional importance, 4 geosites of national importance and 1 geosite of international importance). The basic motif for production of this PhD thesis was to present outstanding natural and cultural-historic values of Golija Mt. –ecological roof of Serbia and Pešter–the largest plateau on the Balkan Peninsula, to a broader scientific, cultural and historical public, as well as to show its great natural, ecotouristic and economic potentials of not only national, but also of international significance

Supplementary data for the article: Pavlović, A. V.; Papetti, A.; Zagorac, D. T. D.; Gašić, U. M.; Mišić, D. M.; Tešić, T. L.; Natić, M. M. Phenolics Composition of Leaf Extracts of Raspberry and Blackberry Cultivars Grown in Serbia. Industrial Crops and Products 2016, 87, 304–314. https://doi.org/10.1016/j.indcrop.2016.04.052

Supplementary material for: [https://doi.org/10.1016/j.indcrop.2016.04.052]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2257

Chemical markers for the authentication of unifloral Salvia officinalis L. honey

The objective of the present study was to trace phytochemicals that characterize unifloral Common sage (Salvia officinalis L.) honey originating from the Croatian North Adriatic coast. The polyphenolic profiles and total phenolic contents (TPC), the compositions of minerals, sugars and sugar alcohols, and the radical scavenging activities (RSA) of 18 unifloral S. officinalis honey samples were investigated. The quantitative data on the targeted compounds (25 phenolic compounds, 14 carbohydrates and 25 minerals) together with the TPC and RSA data served as a pool of variables for multivariate analysis, which provided useful information for the accurate authentication of unifloral sage honey and its discrimination from other unifloral types of honey. The proposed markers, together with chemometrics, could further contribute, as a powerful tool, to the quality control of Croatian unifloral S. officinalis honey and thus, possibly certify its commercial value.This is the peer-reviewed version of the following article: Gašić, U. M.; Natić, M. M.; Mišić, D. M.; Lušić, D. V.; Milojković-Opsenica, D. M.; Tešić, T. L.; Lušić, D. Chemical Markers for the Authentication of Unifloral Salvia Officinalis L. Honey. Journal of Food Composition and Analysis 2015, 44, 128–138. [https://doi.org/10.1016/j.jfca.2015.08.008]Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3353

Supplementary data for the article: Pavlović, A. V.; Papetti, A.; Zagorac, D. T. D.; Gašić, U. M.; Mišić, D. M.; Tešić, T. L.; Natić, M. M. Phenolics Composition of Leaf Extracts of Raspberry and Blackberry Cultivars Grown in Serbia. Industrial Crops and Products 2016, 87, 304–314. https://doi.org/10.1016/j.indcrop.2016.04.052

Supplementary material for: [https://doi.org/10.1016/j.indcrop.2016.04.052]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2257

Supplementary data for article: Gašić, U. M.; Natić, M. M.; Mišić, D. M.; Lušić, D. V.; Milojković-Opsenica, D. M.; Tešić, T. L.; Lušić, D. Chemical Markers for the Authentication of Unifloral Salvia Officinalis L. Honey. Journal of Food Composition and Analysis 2015, 44, 128–138. https://doi.org/10.1016/j.jfca.2015.08.008

Supplementary material for: [https://doi.org/10.1016/j.jfca.2015.08.008]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1983]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3352

Supplementary data for article: Pavlovic, A. V.; Dabić-Zagorac, D.; Momirović, N. M.; Dojčinović, B. P.; Milojković-Opsenica, D.; Tešić, Ž. L.; Natić, M. Chemical Composition of Two Different Extracts of Berries Harvested in Serbia. Journal of Agricultural and Food Chemistry 2013, 61 (17), 4188–4194. https://doi.org/10.1021/jf400607f

Supporting information for: [https://doi.org/10.1021/jf400607f]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1640

Cytotoxicity of Glass Ionomer Cement on Human Exfoliated Deciduous Teeth Stem Cells Correlates with Released Fluoride, Strontium and Aluminum Ion Concentrations

Stem cells from human exfoliated deciduous teeth (SHED) can be used as a cell-based therapy in regenerative medicine and in immunomodulation. Pulp from human deciduous teeth can be stored as a source of SHED. Glass ionomer cements (GICs) are commonly used in restorative dentistry and in cavity lining. GICs have lower biocompatibility and are cytotoxic for dental pulp cells. In this study, seven commonly used GICs were tested for their cytotoxic effects on SHED, for their potential to arrest mitosis in cells and induce chromosome aberrations, and were compared with the effects of composite. Fuji II, Fuji VIII, Fuji IX, Fuji plus and Vitrebond had significantly higher cytotoxic effects on SHED than composite. Only SHEDs that have been treated with Fuji I, Fuji IX, Fuji plus and composite recovered the potential for proliferation, but no chromosome aberrations were found after treatment with GICs. The cytotoxic effects of GICs on SHEDs were in strong correlation with combined concentrations of released fluoride, aluminum and strontium ions. Fuji I exhibited the lowest activity towards SHEDs; it did not interrupt mitosis and did not induce chromosome aberrations, and was accompanied by the lowest levels of released F, Al and Sr ions

Effect of soybean/proso millet intercropping combined with bio-fertilizer on accumulation of essential elements in grain

Intercropping, as sustainable agricultural practice effectively uses environmental resources, affecting the nutritive quality of crops by modifying the concentration of macro- and microelements in grain. In order to improve the availability of nutrients from the soil, bio-fertilizers could also be used successfully in elements management. A field experiment was conducted with soybean (var. Selena) and proso millet (var. Biserka) to examine the integrated influence of intercropping and bio-fertilizer on Ca, Mg, S, Fe and Zn accumulation in grain. Mono-crops (T1 - soybean, T2 - millet), as well as tree planting patterns of intercrop, including alternating rows of soybean and proso millet (T3), alternating strips of two rows of soybean and two rows of millet (T4) and alternating strips of two rows of soybean and four rows of millet (T5), were set up in 2018 and 2020. The same combinations, as subplots, were treated with bio-fertilizer Coveron (C) (containing mycorrhizal fungi, Trichoderma and plant growth-promoting rhizobacteria). After harvest, grains were dried and milled and preparation of samples was performed using wet digestion procedure with HClO4 + HNO3. The concentration of Ca, Mg and S were determined by ICP-OES, while Fe and Zn concentration was measured on ICP-MS. Results showed that Coveron reduced S concentration in soybean grain, while the status of other elements mainly remained unchanged. In terms of intercropping, soybean from T5 combination had a significantly higher level of Fe in comparison to T1 and T3, and higher but not significantly than T4, emphasizing alternating strips as more effective for accumulation of this element in grain. Combination T3 + C provided the highest accumulation of Ca, while T5 + C significantly raised Fe concentration. In terms of proso millet, bio-fertilizer expressed a greater impact on Ca, Fe and Zn by increasing their concentration. On the other side, all intercrop combinations expressed a positive impact on Fe concentration and simultaneously reduced Zn concentration. When mutual influence is considered, T4 + C significantly increased Ca and Fe values, while T2 + C increased Zn value, pointing positive effect of integrated and sustainable agricultural practices on an accumulation of essential elements in a grain of proso millet. In conclusion, different intercrop combinations combined with bio-fertilizer could be successfully used to improve the nutritional quality of soybean and millet grains in terms of sustainable food production, but further investigations are required to highlight other aspects of potentially enhanced nutritional quality

Supplementary data for article: Gašić, U. M.; Natić, M. M.; Mišić, D. M.; Lušić, D. V.; Milojković-Opsenica, D. M.; Tešić, T. L.; Lušić, D. Chemical Markers for the Authentication of Unifloral Salvia Officinalis L. Honey. Journal of Food Composition and Analysis 2015, 44, 128–138. https://doi.org/10.1016/j.jfca.2015.08.008

Supplementary material for: [https://doi.org/10.1016/j.jfca.2015.08.008]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1983]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3352

Supplementary data for article: Pavlovic, A. V.; Dabić-Zagorac, D.; Momirović, N. M.; Dojčinović, B. P.; Milojković-Opsenica, D.; Tešić, Ž. L.; Natić, M. Chemical Composition of Two Different Extracts of Berries Harvested in Serbia. Journal of Agricultural and Food Chemistry 2013, 61 (17), 4188–4194. https://doi.org/10.1021/jf400607f

Supporting information for: [https://doi.org/10.1021/jf400607f]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1640